Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0550
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC25H2.02 |ths1||threonine-tRNA ligase Ths1 |Schizosaccharomyc... 190 2e-49
SPBP23A10.13 |orc4|orp4|origin recognition complex subunit Orc4|... 27 3.4
SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein interm... 26 4.5
SPAC14C4.03 |mek1||Cds1/Rad53/Chk2 family protein kinase Mek1 |S... 26 4.5
SPAC23G3.12c |||serine protease |Schizosaccharomyces pombe|chr 1... 26 6.0
SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schi... 26 6.0
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 25 7.9
SPCC1223.04c |mug76||lysine methyltransferase |Schizosaccharomyc... 25 7.9
SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual 25 7.9
>SPBC25H2.02 |ths1||threonine-tRNA ligase Ths1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 703
Score = 190 bits (462), Expect = 2e-49
Identities = 88/192 (45%), Positives = 128/192 (66%), Gaps = 1/192 (0%)
Frame = +1
Query: 115 YIQKRITLWDKYKAQYTEQIASKPDISVVVTLPDGKTVEAKAWKTTPYDVAKGISQGLAD 294
+IQ R+ L+DK + +Y E +A+KP + +TLPDGK ++ +W+TTP +A IS+GLAD
Sbjct: 22 FIQHRLDLFDKLQKEYKESLATKPREEIDITLPDGKVIKGTSWETTPISIAASISKGLAD 81
Query: 295 ATIIARVNNELWDLDRPL*GDCKLELLRWDNTDAQAVFWHSSAHMLGEAMERVYGGCLCY 474
+A VN E WDL RPL C L+L +++ + + VFWHSSAH+LGEA E + LC
Sbjct: 82 RVTVAIVNGEPWDLTRPLEASCTLKLCDFNDPEGKRVFWHSSAHILGEATELSFHCHLCI 141
Query: 475 GPPIEEGFYYDMYYPE-KGISSTDFNVLEGLIKKIAKEKQPFERLELTKEQLLEMFDYNP 651
GPP +EGF+Y+M + I++ D++ +E K+ K+KQPFERL ++KE LLEMF YN
Sbjct: 142 GPPTDEGFFYEMGIDNGRVITNDDYSSIESYAKQAIKQKQPFERLVISKEGLLEMFRYNK 201
Query: 652 FKVRILNEESSD 687
+K I+ + D
Sbjct: 202 YKQYIIQTKIPD 213
>SPBP23A10.13 |orc4|orp4|origin recognition complex subunit
Orc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 972
Score = 26.6 bits (56), Expect = 3.4
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = -3
Query: 626 NCSFVSSKRSKGCFSFAIFFIRPSSTLKSVEDIPFSG*YMS 504
+CS KR K FS + +RP S+L E I S Y+S
Sbjct: 723 DCSESLEKRVKSRFSHMVIPMRPPSSLSEFEQILKSVLYVS 763
>SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein
intermediate chain Dic1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 544
Score = 26.2 bits (55), Expect = 4.5
Identities = 18/71 (25%), Positives = 31/71 (43%)
Frame = +1
Query: 130 ITLWDKYKAQYTEQIASKPDISVVVTLPDGKTVEAKAWKTTPYDVAKGISQGLADATIIA 309
+ LW ++ ++ D+ V + KT KA +DV +S+ A++ A
Sbjct: 428 VRLWQCSPSRNQHELVPSNDLDEQVIINSCKTFTHKAMV---FDVKWCVSEPCCFASVDA 484
Query: 310 RVNNELWDLDR 342
N LWDL +
Sbjct: 485 LGNLNLWDLQK 495
>SPAC14C4.03 |mek1||Cds1/Rad53/Chk2 family protein kinase Mek1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 445
Score = 26.2 bits (55), Expect = 4.5
Identities = 9/15 (60%), Positives = 13/15 (86%)
Frame = -1
Query: 361 YSLLIEADLDPTVHC 317
YS+LI+ D+DP V+C
Sbjct: 87 YSVLIDDDMDPLVYC 101
>SPAC23G3.12c |||serine protease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 996
Score = 25.8 bits (54), Expect = 6.0
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +1
Query: 166 EQIASKPDISVVVTLPDGKTVEAKAWKTTPYDVAKGI 276
E A+K +V+ P G E+K WK + V K +
Sbjct: 27 EHEATKSSETVIEMTPSGPIAESKKWKESIARVVKSV 63
>SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA
reductase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1053
Score = 25.8 bits (54), Expect = 6.0
Identities = 14/52 (26%), Positives = 26/52 (50%)
Frame = -2
Query: 504 IVESFFYGRPIA*TSSIYSFHSFSKHMSRRVPEDSLCVGIVPP*QLQLTVSL 349
++ + FYG ++ TS + + HSF + S + L IV L + V++
Sbjct: 493 LLPAVFYGAELSSTSFLSTIHSFINNWSHYISASFLSKWIVCALSLSIAVNV 544
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 25.4 bits (53), Expect = 7.9
Identities = 16/56 (28%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Frame = +1
Query: 496 FYYDMYYPEKGISSTDFNVLEGLIKKIAKEKQPFERLEL-TKEQLLEMFDYNPFKV 660
F+ D+Y P K I S + L ++ + +K P E L+ + L+ + D+N V
Sbjct: 1405 FFKDLYSPHKEIYSVAIDALRHVLSQ--NQKLPKELLQSGLRPILMNLSDHNKLSV 1458
>SPCC1223.04c |mug76||lysine methyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 381
Score = 25.4 bits (53), Expect = 7.9
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = +2
Query: 401 LSSGTLLLICLEKLWKE 451
+S G+ + IC E+LWK+
Sbjct: 351 ISDGSYVFICAEQLWKD 367
>SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1339
Score = 25.4 bits (53), Expect = 7.9
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +1
Query: 550 VLEGLIKKIAKEKQPFERLELTKEQLLEMFDYNPFKVRILNEESSDSD 693
+L GL K+A E +P E L L + +++ F Y+ + DSD
Sbjct: 934 ILRGL--KLASEGKPLEDLCLVQYDMMQQFCYSNSDINNQTSTFVDSD 979
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,801,085
Number of Sequences: 5004
Number of extensions: 58878
Number of successful extensions: 208
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 195
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 207
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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