Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0550
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g26830.1 68418.m03201 threonyl-tRNA synthetase / threonine--t... 198 3e-51
At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative / th... 149 1e-36
At2g04842.1 68415.m00498 threonyl-tRNA synthetase, putative / th... 40 0.002
At4g12740.1 68417.m02001 adenine-DNA glycosylase-related / MYH-r... 30 1.3
At5g04150.1 68418.m00403 basic helix-loop-helix (bHLH) family pr... 29 2.2
At1g10900.1 68414.m01252 phosphatidylinositol-4-phosphate 5-kina... 29 2.2
At5g65530.1 68418.m08245 protein kinase, putative contains prote... 29 3.9
At5g07650.1 68418.m00876 formin homology 2 domain-containing pro... 28 6.8
At1g59312.1 68414.m06676 hypothetical protein 28 6.8
At1g58936.1 68414.m06664 hypothetical protein 28 6.8
At1g58643.1 68414.m06656 hypothetical protein 28 6.8
At1g03300.1 68414.m00308 agenet domain-containing protein contai... 28 6.8
At5g57450.2 68418.m07178 DNA repair family protein contains simi... 27 9.0
At5g57450.1 68418.m07177 DNA repair family protein contains simi... 27 9.0
At2g16220.1 68415.m01859 F-box family protein 27 9.0
At1g67530.1 68414.m07694 armadillo/beta-catenin repeat family pr... 27 9.0
At1g27910.1 68414.m03420 U-box domain-containing protein contain... 27 9.0
>At5g26830.1 68418.m03201 threonyl-tRNA synthetase / threonine--tRNA
ligase (THRRS) identical to SP|O04630 Threonyl-tRNA
synthetase, mitochondrial precursor (EC 6.1.1.3)
(Threonine--tRNA ligase) (ThrRS) {Arabidopsis thaliana}
Length = 709
Score = 198 bits (483), Expect = 3e-51
Identities = 95/192 (49%), Positives = 129/192 (67%), Gaps = 2/192 (1%)
Frame = +1
Query: 118 IQKRITLWDKYKAQYTEQIASKPDISVVVTLPDGKTVEAKAWKTTPYDVAKGISQGLADA 297
I KRI L+++ +A E + S P + VTLPDG E K W+TTP D+A IS+GLA++
Sbjct: 49 IPKRIKLFEQIQANQLENLKSLPHDPIKVTLPDGNVKEGKKWETTPMDIAAQISKGLANS 108
Query: 298 TIIARVNNELWDLDRPL*GDCKLELLRWDNTDAQAVFWHSSAHMLGEAMERVYGGCLCYG 477
+I+ V++ LWD++RPL GDCKLEL ++D+ + WHSSAH+LG+A+E+ YG LC G
Sbjct: 109 ALISAVDDVLWDMNRPLEGDCKLELFKFDSDKGRDTLWHSSAHILGQALEQEYGCQLCIG 168
Query: 478 PPIE--EGFYYDMYYPEKGISSTDFNVLEGLIKKIAKEKQPFERLELTKEQLLEMFDYNP 651
P EGFYYD +Y E G+S F +E K AKE QPFER+E+TK+Q LEMF N
Sbjct: 169 PCTTRGEGFYYDGFYGELGLSDNHFPSIEAGAAKAAKEAQPFERIEVTKDQALEMFSENN 228
Query: 652 FKVRILNEESSD 687
FKV ++N +D
Sbjct: 229 FKVELINGLPAD 240
>At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative /
threonine--tRNA ligase, putative similar to SP|O04630
Threonyl-tRNA synthetase, mitochondrial precursor (EC
6.1.1.3) (Threonine--tRNA ligase) (ThrRS) {Arabidopsis
thaliana}; contains Pfam profiles PF00587: tRNA
synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain, PF02824: TGS domain
Length = 458
Score = 149 bits (362), Expect = 1e-36
Identities = 70/151 (46%), Positives = 104/151 (68%), Gaps = 4/151 (2%)
Frame = +1
Query: 118 IQKRITLWDKYKAQYTEQIASKPDISVVVTL-PDGKTVEAKAWKTTPYDVAKGISQGLAD 294
I KRI L+++ +A+ EQ+ S+P + +TL PDG E + W+T+P D+A IS+GLA
Sbjct: 17 IPKRIRLFEEIQAEQLEQLQSRPHDPIKITLLPDGIEKEGRRWETSPMDIAVQISKGLAK 76
Query: 295 ATIIARVNNELWDLDRPL*GDCKLELLRWDNTDAQAVFWHSSAHMLGEAMERVYGGCLCY 474
+ +++ VN+ LWD++RPL GDC LE+ +D+ + FWHSSAH+LG+A+E+ YG LC
Sbjct: 77 SALVSSVNHVLWDMNRPLEGDCSLEIFGFDSDQGRNTFWHSSAHILGQALEQEYGCKLCI 136
Query: 475 GP--PIEEGFYYD-MYYPEKGISSTDFNVLE 558
GP P +EGFYYD +YY E G++ F +E
Sbjct: 137 GPCEPRDEGFYYDSLYYGELGLNDNHFPNIE 167
>At2g04842.1 68415.m00498 threonyl-tRNA synthetase, putative /
threonine--tRNA ligase, putative similar to SP|P18256
Threonyl-tRNA synthetase 2 (EC 6.1.1.3) (Threonine--tRNA
ligase) (ThrRS) {Bacillus subtilis}; contains Pfam
profiles PF00587: tRNA synthetase class II core domain
(G, H, P, S and T), PF03129: Anticodon binding domain
Length = 650
Score = 39.9 bits (89), Expect = 0.002
Identities = 24/100 (24%), Positives = 53/100 (53%), Gaps = 3/100 (3%)
Frame = +1
Query: 382 DNTDAQAVFWHSSAHMLGEAMERVYGGC-LCYGPPIEEGFYYDMYYPEKGISSTDFNVLE 558
+++D H+ AH++ A+++++ + GP I+ GFYYD + + ++ D ++
Sbjct: 74 ESSDKLLKIRHTCAHVMAMAVQKLFPDAKVTIGPWIDNGFYYD--FDMEPLTDKDLKRIK 131
Query: 559 GLIKKIAKEKQPFERLELTKEQLLE--MFDYNPFKVRILN 672
+ +I P R E+++E+ + M P+K+ IL+
Sbjct: 132 KEMDRIISRNLPLLREEVSREEAKKRIMAINEPYKMEILD 171
>At4g12740.1 68417.m02001 adenine-DNA glycosylase-related /
MYH-related similar to MYH (GI:18845094) [Rattus
norvegicus]; similar to adenine-DNA glycosylase
(GI:12656850) [Mus musculus]; contains TIGRFAM profile
TIGR01084: A/G-specific adenine glycosylase (hits below
the trusted cutoff)
Length = 630
Score = 30.3 bits (65), Expect = 1.3
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = -3
Query: 377 LSSSNLQSPYRGRSRSHSSLLTLAIIVAS 291
L S ++ PYRG SH LLTL ++ S
Sbjct: 570 LMSHQIEQPYRGNENSHDLLLTLFFMLLS 598
>At5g04150.1 68418.m00403 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain; PMID: 12679534
Length = 240
Score = 29.5 bits (63), Expect = 2.2
Identities = 14/45 (31%), Positives = 25/45 (55%)
Frame = +1
Query: 562 LIKKIAKEKQPFERLELTKEQLLEMFDYNPFKVRILNEESSDSDD 696
++K I ++KQ +RL KE+LL+ + ++ N+ DS D
Sbjct: 110 VVKYIPEQKQELQRLSRRKEELLKRISRKTHQEQLRNKAMMDSID 154
>At1g10900.1 68414.m01252 phosphatidylinositol-4-phosphate 5-kinase
family protein similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 754
Score = 29.5 bits (63), Expect = 2.2
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +1
Query: 358 CKLELLRWDNTDAQAVFWHSSAHMLGEAMERVYGGCLCYG 477
C+ ++RW+N D FW + G + + GCL YG
Sbjct: 141 CERGVMRWENGDLYDGFW-LNGFRHGSGVYKFADGCLYYG 179
>At5g65530.1 68418.m08245 protein kinase, putative contains protein
kinase domain, Pfam:PF00069
Length = 456
Score = 28.7 bits (61), Expect = 3.9
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +1
Query: 208 LPDGKTVEAKAWKTTPYDVAKGISQGLADATIIARVNN 321
LPDG+TV K +V + +S L++ IIA VN+
Sbjct: 163 LPDGETVAIKKLTRHAKEVEERVSDFLSELGIIAHVNH 200
>At5g07650.1 68418.m00876 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02128
Length = 815
Score = 27.9 bits (59), Expect = 6.8
Identities = 22/73 (30%), Positives = 38/73 (52%), Gaps = 1/73 (1%)
Frame = +1
Query: 133 TLWDKYKAQYTE-QIASKPDISVVVTLPDGKTVEAKAWKTTPYDVAKGISQGLADATIIA 309
+LWD+ + ++ + Q A + DIS + TL VEAK K +D+ + + +
Sbjct: 77 SLWDELQRRHGDSQTAIELDISELETL---FFVEAKPEKIRLHDLRRASYRVFNVRSYYM 133
Query: 310 RVNNELWDLDRPL 348
R NN++ +L PL
Sbjct: 134 RANNKVINLSMPL 146
>At1g59312.1 68414.m06676 hypothetical protein
Length = 486
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/41 (29%), Positives = 25/41 (60%)
Frame = +1
Query: 529 ISSTDFNVLEGLIKKIAKEKQPFERLELTKEQLLEMFDYNP 651
+SS+ F E +IKK + ++ L+L + Q+ ++ +Y+P
Sbjct: 209 LSSSSFIAEENVIKKSISRFEMYQVLKLRENQISQISEYDP 249
>At1g58936.1 68414.m06664 hypothetical protein
Length = 486
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/41 (29%), Positives = 25/41 (60%)
Frame = +1
Query: 529 ISSTDFNVLEGLIKKIAKEKQPFERLELTKEQLLEMFDYNP 651
+SS+ F E +IKK + ++ L+L + Q+ ++ +Y+P
Sbjct: 209 LSSSSFIAEENVIKKSISRFEMYQVLKLRENQISQISEYDP 249
>At1g58643.1 68414.m06656 hypothetical protein
Length = 486
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/41 (29%), Positives = 25/41 (60%)
Frame = +1
Query: 529 ISSTDFNVLEGLIKKIAKEKQPFERLELTKEQLLEMFDYNP 651
+SS+ F E +IKK + ++ L+L + Q+ ++ +Y+P
Sbjct: 209 LSSSSFIAEENVIKKSISRFEMYQVLKLRENQISQISEYDP 249
>At1g03300.1 68414.m00308 agenet domain-containing protein contains
Pfam PF05641: Agenet domain
Length = 670
Score = 27.9 bits (59), Expect = 6.8
Identities = 15/36 (41%), Positives = 23/36 (63%), Gaps = 1/36 (2%)
Frame = +1
Query: 556 EGLIKKIAKEKQPFERLELTKEQL-LEMFDYNPFKV 660
+G++K + EKQ LE TK++L ++ D PFKV
Sbjct: 241 QGVVKGVHIEKQYTVTLEATKDKLVVKHSDLRPFKV 276
>At5g57450.2 68418.m07178 DNA repair family protein contains
similarity to Swiss-Prot:O43542 DNA-repair protein XRCC3
(X-ray repair cross-complementing protein 3) [Homo
sapiens]
Length = 304
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/39 (35%), Positives = 26/39 (66%)
Frame = +1
Query: 91 SEMNPWPEYIQKRITLWDKYKAQYTEQIASKPDISVVVT 207
SE + P ++KR +L+ K + +Q+ASK D+++V+T
Sbjct: 161 SEFDNTPSDLKKRSSLFFKISGKL-KQLASKFDLAIVIT 198
>At5g57450.1 68418.m07177 DNA repair family protein contains
similarity to Swiss-Prot:O43542 DNA-repair protein XRCC3
(X-ray repair cross-complementing protein 3) [Homo
sapiens]
Length = 304
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/39 (35%), Positives = 26/39 (66%)
Frame = +1
Query: 91 SEMNPWPEYIQKRITLWDKYKAQYTEQIASKPDISVVVT 207
SE + P ++KR +L+ K + +Q+ASK D+++V+T
Sbjct: 161 SEFDNTPSDLKKRSSLFFKISGKL-KQLASKFDLAIVIT 198
>At2g16220.1 68415.m01859 F-box family protein
Length = 407
Score = 27.5 bits (58), Expect = 9.0
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Frame = +1
Query: 82 NAVSEMNPWP-EYIQKRITLWDKYKAQYTEQIASKPDISVVVTLPDGKTV 228
+A+ EM+ W E ++K+ W KY +TE + +SVV G+ V
Sbjct: 274 DAIDEMHVWVLEDVEKKE--WSKYAYTWTEDKLYRSQVSVVGMTASGEIV 321
>At1g67530.1 68414.m07694 armadillo/beta-catenin repeat family
protein / U-box domain-containing family protein
contains Pfam domain, PF00514:
Armadillo/beta-catenin-like repeats and Pfam, PF04564:
U-box domain
Length = 782
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 1/46 (2%)
Frame = -3
Query: 254 GVVFQALASTVFPS-GNVTTTLISGLDAICSVYCALYLSHNVIRFC 120
GV+ + L+ +FPS SG+ A+CS++ AL + N+++ C
Sbjct: 28 GVLCKVLS--IFPSLEGARPRSKSGIQALCSLHIALEKAKNILQHC 71
>At1g27910.1 68414.m03420 U-box domain-containing protein contains
Pfam profile PF04564: U-box domain
Length = 768
Score = 27.5 bits (58), Expect = 9.0
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = -3
Query: 188 SGLDAICSVYCALYLSHNVIRFCIYSGHGFISET 87
SG+ A+CS++ L N++R C S +++ T
Sbjct: 49 SGIQALCSLHVVLEKVKNILRHCTESSKLYLAIT 82
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,363,816
Number of Sequences: 28952
Number of extensions: 300106
Number of successful extensions: 867
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 841
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 864
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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