Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0549
(709 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g15955.1 68417.m02422 epoxide hydrolase-related similar to ep... 33 0.19
At1g13110.1 68414.m01520 cytochrome P450 71B7 (CYP71B7) identica... 33 0.25
At1g13090.1 68414.m01518 cytochrome P450 71B28, putative (CYP71B... 33 0.25
At2g26740.1 68415.m03207 epoxide hydrolase, soluble (sEH) identi... 32 0.32
At3g26210.1 68416.m03270 cytochrome P450 71B23, putative (CYP71B... 32 0.43
At1g13100.1 68414.m01519 cytochrome P450 71B29, putative (CYP71B... 31 0.57
At5g20930.1 68418.m02486 protein kinase, putative nearly identic... 31 0.75
At1g28060.1 68414.m03435 small nuclear ribonucleoprotein family ... 31 0.75
At3g05600.1 68416.m00622 epoxide hydrolase, putative similar to ... 31 0.99
At3g51000.1 68416.m05584 epoxide hydrolase, putative similar to ... 30 1.7
At4g36530.2 68417.m05187 hydrolase, alpha/beta fold family prote... 29 3.0
At4g36530.1 68417.m05186 hydrolase, alpha/beta fold family prote... 29 3.0
At4g18770.1 68417.m02773 myb family transcription factor (MYB98)... 29 3.0
At2g45340.1 68415.m05642 leucine-rich repeat transmembrane prote... 29 3.0
At1g48150.1 68414.m05374 MADS-box protein (AGL74 ) similar to GB... 29 3.0
At4g12830.1 68417.m02011 hydrolase, alpha/beta fold family prote... 29 4.0
At3g18870.1 68416.m02397 mitochondrial transcription termination... 29 4.0
At5g07060.1 68418.m00799 zinc finger (CCCH-type) family protein ... 28 7.0
At3g16330.1 68416.m02063 expressed protein 27 9.2
At2g26750.1 68415.m03208 epoxide hydrolase, putative strong simi... 27 9.2
>At4g15955.1 68417.m02422 epoxide hydrolase-related similar to
epoxide hydrolase GI:1109600 from [Arabidopsis thaliana]
Length = 178
Score = 33.1 bits (72), Expect = 0.19
Identities = 24/79 (30%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = +2
Query: 434 YPHFKTNIQGLNIHFMRITPKVPKGVEIVP--LLLLHGWPGSVREFYEAIPHLTAVSKDR 607
+ H + G+ +H +P V I P +L LHG+P +Y + A+S
Sbjct: 5 FDHSFVKVNGITMHVAEKSPSVAGNGAIRPPVILFLHGFP---ELWYTWRHQMVALS--- 58
Query: 608 NFALEIIAPSLPGYGFSDA 664
+ IAP L GYG +DA
Sbjct: 59 SLGYRTIAPDLRGYGDTDA 77
>At1g13110.1 68414.m01520 cytochrome P450 71B7 (CYP71B7) identical
to (SP:Q96514) cytochrome P450 71B7 [Arabidopsis
thaliana]; PF|00067 Cytochrome P450 family. ESTs
gb|T44875, gb|T04814, gb|R65111, gb|T44310 and gb|T04541
come from this gene; identical to cDNA cytochrome P450
GI:1523795, ATCYP71B7
Length = 504
Score = 32.7 bits (71), Expect = 0.25
Identities = 18/38 (47%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = -1
Query: 496 LWSNPHEVNIQT-LDISLEMRILVKELLPFSEWVFFCP 386
LW+NP E N LD S++ R L ELLPF CP
Sbjct: 410 LWTNPDEFNPDRFLDSSIDYRGLNFELLPFGSGRRICP 447
>At1g13090.1 68414.m01518 cytochrome P450 71B28, putative (CYP71B28)
Identical to Cytochrome P450 (SP:Q9SAE3) [Arabidopsis
thaliana]; strong similarity to gb|X97864 cytochrome
P450 from Arabidopsis thaliana and is a member of the
PF|00067 Cytochrome P450 family. ESTs gb|N65665,
gb|T14112, gb|T76255, gb|T20906 and gb|AI100027 come
from this gene
Length = 490
Score = 32.7 bits (71), Expect = 0.25
Identities = 18/38 (47%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = -1
Query: 496 LWSNPHEVNIQT-LDISLEMRILVKELLPFSEWVFFCP 386
LW+NP E N LD S++ R L ELLPF CP
Sbjct: 404 LWTNPDEFNPDRFLDSSIDYRGLNFELLPFGSGRRICP 441
>At2g26740.1 68415.m03207 epoxide hydrolase, soluble (sEH) identical
to ATsEH [Arabidopsis thaliana] GI:1109600
Length = 321
Score = 32.3 bits (70), Expect = 0.32
Identities = 28/75 (37%), Positives = 36/75 (48%)
Frame = +2
Query: 440 HFKTNIQGLNIHFMRITPKVPKGVEIVPLLLLHGWPGSVREFYEAIPHLTAVSKDRNFAL 619
H K G++IH + + P IV LLLHG+P + IP L A R +
Sbjct: 3 HRKVRGNGIDIH---VAIQGPSDGPIV--LLLHGFPELWYSWRHQIPGLAA----RGY-- 51
Query: 620 EIIAPSLPGYGFSDA 664
+AP L GYG SDA
Sbjct: 52 RAVAPDLRGYGDSDA 66
>At3g26210.1 68416.m03270 cytochrome P450 71B23, putative (CYP71B23)
Identical to Cytochrome P450 71B23
(SP:Q9LTM0)[Arabidopsis thaliana];contains Pfam profile:
PF00067 cytochrome P450
Length = 501
Score = 31.9 bits (69), Expect = 0.43
Identities = 17/39 (43%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = -1
Query: 499 DLWSNPHEVNIQT-LDISLEMRILVKELLPFSEWVFFCP 386
DLW NP E + +D S++ R L ELLPF CP
Sbjct: 406 DLWENPEEFKPERFVDSSVDYRGLNFELLPFGSGRRICP 444
>At1g13100.1 68414.m01519 cytochrome P450 71B29, putative (CYP71B29)
strong similarity to gb|X97864 cytochrome P450 and
identical to Cytochrome P450 71B29
(SP:Q9SAE4)[Arabidopsis thaliana];PF|00067 Cytochrome
P450 family
Length = 490
Score = 31.5 bits (68), Expect = 0.57
Identities = 17/38 (44%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = -1
Query: 496 LWSNPHEVNIQT-LDISLEMRILVKELLPFSEWVFFCP 386
LW+NP E N LD S++ + L ELLPF CP
Sbjct: 404 LWTNPDEFNPDRFLDSSIDYKGLNFELLPFGSGRRICP 441
>At5g20930.1 68418.m02486 protein kinase, putative nearly identical
to protein kinase tousled gi|433052|gb|AAA32874
Length = 688
Score = 31.1 bits (67), Expect = 0.75
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = -3
Query: 248 RRV*CWYPVSV*VRAVPTTPPGLAEASAVDSSE 150
++V W S V+ V +TPPGL+E S DS +
Sbjct: 51 QQVSLWSSASAAVKVVTSTPPGLSETSISDSDD 83
>At1g28060.1 68414.m03435 small nuclear ribonucleoprotein family
protein / snRNP family protein contains similarity to
U4/U6 small nuclear ribonucleoprotein hPrp3 [Homo
sapiens] gi|2708307|gb|AAC51926
Length = 786
Score = 31.1 bits (67), Expect = 0.75
Identities = 19/58 (32%), Positives = 25/58 (43%)
Frame = +2
Query: 155 KSPPPMPQLDLEEWWGPPELKQKQDTSIKPFEITFSETMVKELKERIKKRRPFAPPLE 328
K P P D+E WW L + I IT S +++L I+ RP PP E
Sbjct: 483 KEKPKEPIPDVE-WWDANVLTNGEYGEITDGTITESHLKIEKLTHYIEHPRPIEPPAE 539
>At3g05600.1 68416.m00622 epoxide hydrolase, putative similar to
epoxide hydrolase from [Glycine max] GI:2764806,
[Arabidopsis thaliana] GI:1109600; contains Pfam profile
PF00561: hydrolase, alpha/beta fold family
Length = 331
Score = 30.7 bits (66), Expect = 0.99
Identities = 24/75 (32%), Positives = 36/75 (48%)
Frame = +2
Query: 440 HFKTNIQGLNIHFMRITPKVPKGVEIVPLLLLHGWPGSVREFYEAIPHLTAVSKDRNFAL 619
H ++ G+ +H I K PK +V LLLHG+P + I L+++
Sbjct: 6 HRMVSVNGITMH---IAEKGPKEGPVV--LLLHGFPDLWYTWRHQISGLSSLG------Y 54
Query: 620 EIIAPSLPGYGFSDA 664
+AP L GYG SD+
Sbjct: 55 RAVAPDLRGYGDSDS 69
>At3g51000.1 68416.m05584 epoxide hydrolase, putative similar to
epoxide hydrolase [Glycine max] GI:2764806; contains
Pfam profile PF00561: hydrolase, alpha/beta fold family
Length = 323
Score = 29.9 bits (64), Expect = 1.7
Identities = 21/55 (38%), Positives = 29/55 (52%), Gaps = 1/55 (1%)
Frame = +2
Query: 503 KGVEIVPL-LLLHGWPGSVREFYEAIPHLTAVSKDRNFALEIIAPSLPGYGFSDA 664
KG E PL LLLHG+P + + I L++ ++AP L GYG SD+
Sbjct: 22 KGDEEGPLVLLLHGFPETWYSWRHQIDFLSS------HGYHVVAPDLRGYGDSDS 70
>At4g36530.2 68417.m05187 hydrolase, alpha/beta fold family protein
low similarity to hydrolase [Sphingomonas sp.]
GI:3426124; contains Pfam profile PF00561: hydrolase,
alpha/beta fold family
Length = 378
Score = 29.1 bits (62), Expect = 3.0
Identities = 18/50 (36%), Positives = 30/50 (60%)
Frame = +2
Query: 521 PLLLLHGWPGSVREFYEAIPHLTAVSKDRNFALEIIAPSLPGYGFSDAAV 670
PL+L+HG+ SV + IP L K + +AL+++ G+G+SD A+
Sbjct: 101 PLVLIHGFGASVFHWRYNIPEL--AKKYKVYALDLL-----GFGWSDKAL 143
>At4g36530.1 68417.m05186 hydrolase, alpha/beta fold family protein
low similarity to hydrolase [Sphingomonas sp.]
GI:3426124; contains Pfam profile PF00561: hydrolase,
alpha/beta fold family
Length = 321
Score = 29.1 bits (62), Expect = 3.0
Identities = 18/50 (36%), Positives = 30/50 (60%)
Frame = +2
Query: 521 PLLLLHGWPGSVREFYEAIPHLTAVSKDRNFALEIIAPSLPGYGFSDAAV 670
PL+L+HG+ SV + IP L K + +AL+++ G+G+SD A+
Sbjct: 44 PLVLIHGFGASVFHWRYNIPEL--AKKYKVYALDLL-----GFGWSDKAL 86
>At4g18770.1 68417.m02773 myb family transcription factor (MYB98)
identical to transcription factor (MYB98) GI:15375282
from [Arabidopsis thaliana]
Length = 427
Score = 29.1 bits (62), Expect = 3.0
Identities = 17/43 (39%), Positives = 24/43 (55%), Gaps = 3/43 (6%)
Frame = +3
Query: 528 CYSMDG---RALSGSSTKPFLISQLSAKTGTLLWKLSLQVYLV 647
C S D RA S + TKPFL +LS+ + + WK + + LV
Sbjct: 174 CVSADNDCYRATSFNKTKPFLTRKLSSSSSSSSWKETKKSTLV 216
>At2g45340.1 68415.m05642 leucine-rich repeat transmembrane protein
kinase, putative
Length = 691
Score = 29.1 bits (62), Expect = 3.0
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +2
Query: 449 TNIQGLNIHFMRITPKVPKGVEIVPLL 529
T++ GL +HF +T +PK + +PLL
Sbjct: 92 TSLTGLYLHFNSLTGHIPKDISNLPLL 118
>At1g48150.1 68414.m05374 MADS-box protein (AGL74 ) similar to
GB:CAA73304 from [Arabidopsis thaliana] (Gene 215 (1),
11-17 (1998)); contains Pfam domain PF00319: SRF-type
transcription factor (DNA-binding and dimerisation
domain); PMID: 12837945
Length = 323
Score = 29.1 bits (62), Expect = 3.0
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = -2
Query: 405 NGYSSAQYLSQLSNCFELNPYLKPTPSNGGAKGLRFLMRSFSSF 274
N YSS + SQ++ CF+ NP S + R+L+ S F
Sbjct: 193 NSYSSLEPSSQVTTCFDQNPVFS-VGSESSSDQSRYLVNEDSGF 235
>At4g12830.1 68417.m02011 hydrolase, alpha/beta fold family protein
low similarity to haloalkane dehalogenase from
[Mycobacterium avium subsp. avium] GI:14422311,
[Pseudomonas pavonaceae] GI:6689030; contains Pfam
profile PF00561: hydrolase, alpha/beta fold family
Length = 393
Score = 28.7 bits (61), Expect = 4.0
Identities = 17/51 (33%), Positives = 28/51 (54%)
Frame = +2
Query: 509 VEIVPLLLLHGWPGSVREFYEAIPHLTAVSKDRNFALEIIAPSLPGYGFSD 661
V+ P++L+HG+P + + IP L+ +N+ IA G+GFSD
Sbjct: 131 VDSPPVILIHGFPSQAYSYRKTIPVLS-----KNY--RAIAFDWLGFGFSD 174
>At3g18870.1 68416.m02397 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 274
Score = 28.7 bits (61), Expect = 4.0
Identities = 10/26 (38%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
Frame = -1
Query: 412 FSEWVFFCP-IFEPTVQLFRIEPIFE 338
F VF CP +F PT + +++P+F+
Sbjct: 82 FPRLVFLCPQLFSPTFDISKLDPVFD 107
>At5g07060.1 68418.m00799 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 363
Score = 27.9 bits (59), Expect = 7.0
Identities = 14/45 (31%), Positives = 25/45 (55%)
Frame = -2
Query: 408 ANGYSSAQYLSQLSNCFELNPYLKPTPSNGGAKGLRFLMRSFSSF 274
A+G+ SA + +CF NPY++ T ++ K + R F++F
Sbjct: 8 ADGWESADFPITCESCFGDNPYMRMTRADYD-KECKICSRPFTAF 51
>At3g16330.1 68416.m02063 expressed protein
Length = 205
Score = 27.5 bits (58), Expect = 9.2
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +2
Query: 614 ALEIIAPSLPGYGFSDAAVRP 676
AL ++P LPG+G S ++VRP
Sbjct: 137 ALTALSPYLPGFGRSTSSVRP 157
>At2g26750.1 68415.m03208 epoxide hydrolase, putative strong
similarity to ATsEH [Arabidopsis thaliana] GI:1109600
Length = 320
Score = 27.5 bits (58), Expect = 9.2
Identities = 25/75 (33%), Positives = 35/75 (46%)
Frame = +2
Query: 440 HFKTNIQGLNIHFMRITPKVPKGVEIVPLLLLHGWPGSVREFYEAIPHLTAVSKDRNFAL 619
H G++IH + + P IV LLLHG+P E + + H + R +
Sbjct: 3 HRNVRGNGIDIH---VAIQGPSDGTIV--LLLHGFP----ELWYSWRHQISGLAARGY-- 51
Query: 620 EIIAPSLPGYGFSDA 664
+AP L GYG SDA
Sbjct: 52 RAVAPDLRGYGDSDA 66
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,846,289
Number of Sequences: 28952
Number of extensions: 340330
Number of successful extensions: 993
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 966
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 992
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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