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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0548
         (578 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g49360.1 68418.m06108 glycosyl hydrolase family 3 protein           33   0.10 
At1g06800.1 68414.m00724 lipase class 3 family protein similar t...    28   5.2  
At3g51470.1 68416.m05637 protein phosphatase 2C, putative / PP2C...    27   9.0  
At1g02650.1 68414.m00215 DNAJ heat shock N-terminal domain-conta...    27   9.0  

>At5g49360.1 68418.m06108 glycosyl hydrolase family 3 protein
          Length = 774

 Score = 33.5 bits (73), Expect = 0.10
 Identities = 28/97 (28%), Positives = 45/97 (46%), Gaps = 3/97 (3%)
 Frame = +2

Query: 14  KKIWSNHHISVLLFLAVTLVHSSEGHAKVFTRCQLSRELLR-YNFPRALIPTWVCLIEHM 190
           K +   + + V+L   + LVHSSE    +F  C  +  L R   F RA +P  V  ++ +
Sbjct: 6   KALLIGNKVVVILVFLLCLVHSSESLRPLFA-CDPANGLTRTLRFCRANVPIHV-RVQDL 63

Query: 191 ISRTT--EKITNHNNSYSSYGLFQINNKDWCKKGRKG 295
           + R T  EKI N  N+ ++     I   +W  +   G
Sbjct: 64  LGRLTLQEKIRNLVNNAAAVPRLGIGGYEWWSEALHG 100


>At1g06800.1 68414.m00724 lipase class 3 family protein similar to
           DEFECTIVE IN ANTHER DEHISCENCE1 [Arabidopsis thaliana]
           GI:16215706; contains Pfam profile PF01764: Lipase
          Length = 515

 Score = 27.9 bits (59), Expect = 5.2
 Identities = 15/43 (34%), Positives = 20/43 (46%)
 Frame = +3

Query: 354 DAQNGYTIESDSRPGRLLTPTASRRTYQTSPDAERLYQRYHHP 482
           DA  G    +D   GR + P   R   Q +PD  +L  + HHP
Sbjct: 472 DANKGMVRNTD---GRWIQPDRIRADDQHAPDIHQLLTQLHHP 511


>At3g51470.1 68416.m05637 protein phosphatase 2C, putative / PP2C,
           putative protein phosphatase-2C, Mesembryanthemum
           crystallinum, EMBL:AF075580
          Length = 361

 Score = 27.1 bits (57), Expect = 9.0
 Identities = 11/22 (50%), Positives = 12/22 (54%)
 Frame = +2

Query: 269 DWCKKGRKGGNCNMKCEDLLNE 334
           DW  KG KG  C + CE  L E
Sbjct: 239 DWHIKGTKGSLCPLSCEPELEE 260


>At1g02650.1 68414.m00215 DNAJ heat shock N-terminal
           domain-containing protein contains Pfam profile PF00226:
           DnaJ domain
          Length = 513

 Score = 27.1 bits (57), Expect = 9.0
 Identities = 13/37 (35%), Positives = 18/37 (48%)
 Frame = +2

Query: 134 RYNFPRALIPTWVCLIEHMISRTTEKITNHNNSYSSY 244
           R+N     IP  +C++   I +  EKITN  N    Y
Sbjct: 334 RHNVRYREIPGKLCVLTTNIKQMKEKITNRENGNEDY 370


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,025,396
Number of Sequences: 28952
Number of extensions: 239194
Number of successful extensions: 701
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 691
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 701
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1131744440
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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