Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0543
(705 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g80310.1 68414.m09402 expressed protein 31 0.56
At2g06990.1 68415.m00800 HUA enhancer 2 (HEN2) / DExH-box RNA he... 31 0.74
At5g65090.1 68418.m08187 endonuclease/exonuclease/phosphatase fa... 29 2.3
At4g20770.1 68417.m03016 pentatricopeptide (PPR) repeat-containi... 29 4.0
At4g11460.1 68417.m01844 protein kinase family protein contains ... 28 5.2
At1g80630.1 68414.m09462 leucine-rich repeat family protein 28 5.2
At5g19090.2 68418.m02270 heavy-metal-associated domain-containin... 28 6.9
At5g19090.1 68418.m02269 heavy-metal-associated domain-containin... 28 6.9
At1g20760.1 68414.m02600 calcium-binding EF hand family protein ... 28 6.9
At5g09950.1 68418.m01150 pentatricopeptide (PPR) repeat-containi... 27 9.2
At4g05310.1 68417.m00809 ubiquitin family protein contains INTER... 27 9.2
At3g53240.1 68416.m05868 leucine-rich repeat family protein cont... 27 9.2
At1g55900.1 68414.m06411 NLI interacting factor (NIF) family pro... 27 9.2
>At1g80310.1 68414.m09402 expressed protein
Length = 464
Score = 31.5 bits (68), Expect = 0.56
Identities = 16/36 (44%), Positives = 22/36 (61%)
Frame = -2
Query: 683 DSVLMCCLRSSGMFVGRSSVLLYGCGLQLYILLSLR 576
DS +M + M G S+ L +GCG+ LY+LL LR
Sbjct: 402 DSFIMLVCAAVSM-TGSSAALGFGCGVVLYLLLKLR 436
>At2g06990.1 68415.m00800 HUA enhancer 2 (HEN2) / DExH-box RNA
helicase, putative nearly identical to HUA enhancer 2
[Arabidopsis thaliana] GI:16024936
Length = 995
Score = 31.1 bits (67), Expect = 0.74
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +1
Query: 349 KGSVLKWARVIDEFLNQYRKKGSGSGEA 432
+GS+++ AR +DEFLNQ R GE+
Sbjct: 943 EGSIIRSARRLDEFLNQLRAAAEAVGES 970
>At5g65090.1 68418.m08187 endonuclease/exonuclease/phosphatase
family protein similar to inositol polyphosphate
5-phosphatase I (GI:10444261) and II (GI:10444263)
[Arabidopsis thaliana]; contains Pfam profile PF03372:
Endonuclease/Exonuclease/phosphatase family
Length = 529
Score = 29.5 bits (63), Expect = 2.3
Identities = 14/44 (31%), Positives = 21/44 (47%)
Frame = +1
Query: 340 VNDKGSVLKWARVIDEFLNQYRKKGSGSGEAHGAENRVPCSPSS 471
V D KW +I + LN+ +++ S A+ A CS SS
Sbjct: 124 VEDNEPAAKWLALISQALNKPKQESVYSNAAYSASRTTTCSSSS 167
>At4g20770.1 68417.m03016 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 740
Score = 28.7 bits (61), Expect = 4.0
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -3
Query: 475 VRCWESMVRGFRHHEL 428
+ CW SM+ GFRH+ L
Sbjct: 449 IACWNSMISGFRHNML 464
>At4g11460.1 68417.m01844 protein kinase family protein contains
Pfam PF00069: Protein kinase domain
Length = 700
Score = 28.3 bits (60), Expect = 5.2
Identities = 19/70 (27%), Positives = 23/70 (32%)
Frame = +1
Query: 457 CSPSSGPLGEKQVCDVPVDDFNPCTPANQYNYEQAGPCVFLKLNKIYNWRPQPYNNTEDL 636
C+P P VDD+ C Q Y C+F W P+N DL
Sbjct: 204 CTPDVSPSNCNTCLKQSVDDYVGCCHGKQGGYVYRPSCIF-------RWDLYPFNGAFDL 256
Query: 637 PTNMPEDLKQ 666
T P Q
Sbjct: 257 LTLAPPPSSQ 266
>At1g80630.1 68414.m09462 leucine-rich repeat family protein
Length = 578
Score = 28.3 bits (60), Expect = 5.2
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = -2
Query: 326 RVLSTLAVSGIGLKPRPGLL 267
R L +LA++GIGL+PR LL
Sbjct: 215 RNLESLAINGIGLRPRESLL 234
>At5g19090.2 68418.m02270 heavy-metal-associated domain-containing
protein contains Pfam heavy-metal-associated domain
PF00403; glycine-rich protein GRP22, rape, PIR:S31415;
isoform contains a non-consensus TG-acceptor splice site
at intron 3
Length = 465
Score = 27.9 bits (59), Expect = 6.9
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
Frame = +3
Query: 57 REMGGVQDL--PVQLGDGPGPRAHRLKLGKDP 146
++M G QDL P QL GPGP + + K+P
Sbjct: 168 QQMKGFQDLKLPPQLKGGPGPGPGSVPMNKNP 199
>At5g19090.1 68418.m02269 heavy-metal-associated domain-containing
protein contains Pfam heavy-metal-associated domain
PF00403; glycine-rich protein GRP22, rape, PIR:S31415;
isoform contains a non-consensus TG-acceptor splice site
at intron 3
Length = 587
Score = 27.9 bits (59), Expect = 6.9
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
Frame = +3
Query: 57 REMGGVQDL--PVQLGDGPGPRAHRLKLGKDP 146
++M G QDL P QL GPGP + + K+P
Sbjct: 168 QQMKGFQDLKLPPQLKGGPGPGPGSVPMNKNP 199
>At1g20760.1 68414.m02600 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 1019
Score = 27.9 bits (59), Expect = 6.9
Identities = 14/43 (32%), Positives = 20/43 (46%)
Frame = +1
Query: 418 GSGEAHGAENRVPCSPSSGPLGEKQVCDVPVDDFNPCTPANQY 546
GSG+ G SPSS G ++ DD P TP +++
Sbjct: 833 GSGDDFGGNTARADSPSSRSFGAQRKSQFAFDDSVPSTPLSRF 875
>At5g09950.1 68418.m01150 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 995
Score = 27.5 bits (58), Expect = 9.2
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -3
Query: 505 VHHRLASRRVVRCWESMVRGFRHHEL 428
+ R+A RR W SM+ G+ H+EL
Sbjct: 574 IFSRMAERRDNVTWNSMISGYIHNEL 599
>At4g05310.1 68417.m00809 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 415
Score = 27.5 bits (58), Expect = 9.2
Identities = 16/63 (25%), Positives = 30/63 (47%), Gaps = 3/63 (4%)
Frame = +1
Query: 433 HGAENRVPCSPSSGPLGEKQVCDVPVDDFNPCTP---ANQYNYEQAGPCVFLKLNKIYNW 603
H ++ ++ SP P+ +P + +P P +Y EQ P ++ KIY++
Sbjct: 64 HDSQIQLEVSPDVNPIHNNDD-QIPQTEQSPPAPWISVEEYFAEQDWPLTEEEIRKIYSY 122
Query: 604 RPQ 612
RP+
Sbjct: 123 RPE 125
>At3g53240.1 68416.m05868 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 891
Score = 27.5 bits (58), Expect = 9.2
Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = -1
Query: 585 ELEEDARAGLFVIVLVGWSAGIEVIDWYITDLL-LAEWSAAGRAWYAVFGT 436
E E+D +GL IV++ WS G + + L+ L S RAW+ + T
Sbjct: 829 EYEDDDESGLLDIVVLWWSLGTTYVTVMMGFLVFLCFDSPWRRAWFCLVDT 879
>At1g55900.1 68414.m06411 NLI interacting factor (NIF) family
protein contains Pfam profile PF03031: NLI interacting
factor
Length = 376
Score = 27.5 bits (58), Expect = 9.2
Identities = 26/104 (25%), Positives = 42/104 (40%), Gaps = 2/104 (1%)
Frame = -1
Query: 531 SAGIEVIDWYITDLLLAEWSAAGRA--WYAVFGTMSFTTAAAFLTVLIQKFVNNPGPFEH 358
S+GI+VID Y T L A + + RA Y + F L +K + + P E
Sbjct: 129 SSGIDVIDKYQTKLYSAAMTGSARAIDKYLELREIVEEQVKGFTEPLSEKLLPDLHPAEQ 188
Query: 357 RSLIVDLVIDQSTFHVGSIWHWPEAQTWITADY*TIKLPLRHLG 226
+ L ++++ + W + W T + L HLG
Sbjct: 189 HVFTLVLDLNETLLYT----DWKRERGWRTFKRPGVDAFLEHLG 228
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.315 0.134 0.422
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,269,654
Number of Sequences: 28952
Number of extensions: 362331
Number of successful extensions: 773
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 753
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 773
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
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