Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0533
(679 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 44 9e-05
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 43 2e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 41 7e-04
At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17... 39 0.003
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 37 0.011
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 37 0.014
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 34 0.075
At1g78910.1 68414.m09199 pseudouridine synthase family protein l... 29 2.8
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 3.7
At3g49540.1 68416.m05414 expressed protein 28 4.9
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 27 8.6
At1g34180.1 68414.m04239 no apical meristem (NAM) family protein... 27 8.6
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 44.0 bits (99), Expect = 9e-05
Identities = 32/93 (34%), Positives = 50/93 (53%), Gaps = 8/93 (8%)
Frame = +2
Query: 230 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 385
D+ EE+ V+ DG I+ + G+ +EEK D+ + R +FTRR+ LPE E
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 386 SVESRLSSDGVLSVIAPRKVPPAVEGERKIPIA 484
+++ + +GVLSV P KVP + I I+
Sbjct: 126 EIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 43.2 bits (97), Expect = 2e-04
Identities = 31/104 (29%), Positives = 56/104 (53%), Gaps = 8/104 (7%)
Frame = +2
Query: 197 KSDKDKFQVNLDVQHFAPEEISVKTAD-GYIVVEG---KHEEKK-DQHGYISR---QFTR 352
K + ++ LD+ +E+ ++ + G + V G + EEKK DQ + R +F R
Sbjct: 76 KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135
Query: 353 RYALPEGCTAESVESRLSSDGVLSVIAPRKVPPAVEGERKIPIA 484
++ LP+ ESV+++L +GVL++ + P V+G R + IA
Sbjct: 136 QFKLPDNVDMESVKAKL-ENGVLTINLTKLSPEKVKGPRVVNIA 178
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 41.1 bits (92), Expect = 7e-04
Identities = 28/78 (35%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +2
Query: 230 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 385
DV EE+ V+ DG I+ + G+ +EEK D + R +F RR+ LPE E
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 386 SVESRLSSDGVLSVIAPR 439
V++ + +GVLSV P+
Sbjct: 125 EVKASM-ENGVLSVTVPK 141
>At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17.6A
(HSP17.7-CII) identical to heat shock protein 17.6A
GI:3256075 from [Arabidopsis thaliana]
Length = 156
Score = 39.1 bits (87), Expect = 0.003
Identities = 34/126 (26%), Positives = 58/126 (46%), Gaps = 9/126 (7%)
Frame = +2
Query: 131 SREYYRPWRHLAAAARDLGSSIKSDKDKFQVNLDVQHFAPEEISVKTA-DGYIVVEGKHE 307
SR Y R + +AA D + D + +D+ +EI V+ + +VV GK +
Sbjct: 33 SRAYMRDAKAMAATPAD----VIEHPDAYVFAVDMPGIKGDEIQVQIENENVLVVSGKRQ 88
Query: 308 -EKKDQHGY----ISRQ---FTRRYALPEGCTAESVESRLSSDGVLSVIAPRKVPPAVEG 463
+ K+ G + R+ F R++ LP+ E + S +DGVL V P+ PP +
Sbjct: 89 RDNKENEGVKFVRMERRMGKFMRKFQLPDNADLEKI-SAACNDGVLKVTIPKLPPPEPKK 147
Query: 464 ERKIPI 481
+ I +
Sbjct: 148 PKTIQV 153
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 37.1 bits (82), Expect = 0.011
Identities = 24/78 (30%), Positives = 43/78 (55%), Gaps = 8/78 (10%)
Frame = +2
Query: 230 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 385
D+ EE+ V+ D ++ + G+ +EEK D+ + R +F RR+ LPE E
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 386 SVESRLSSDGVLSVIAPR 439
V++ + +GVL+V+ P+
Sbjct: 128 EVKATM-ENGVLTVVVPK 144
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 36.7 bits (81), Expect = 0.014
Identities = 25/78 (32%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +2
Query: 230 DVQHFAPEEISVKTA-DGYIVVEG-KHEEKKDQHGYISR------QFTRRYALPEGCTAE 385
D+ EE+ V+ D + + G +H EK+D++ R QFTRR+ LPE +
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 386 SVESRLSSDGVLSVIAPR 439
V++ + +GVL+V P+
Sbjct: 122 QVKAAM-ENGVLTVTVPK 138
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 34.3 bits (75), Expect = 0.075
Identities = 23/78 (29%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +2
Query: 230 DVQHFAPEEISVKTADGYIV-VEGKH----EEKKDQHGYISR---QFTRRYALPEGCTAE 385
D+ EE+ V+ D ++ + G+ EEK+D + R QF+R++ LPE +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 386 SVESRLSSDGVLSVIAPR 439
V++ + +GVL+V P+
Sbjct: 124 QVKASM-ENGVLTVTVPK 140
>At1g78910.1 68414.m09199 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 478
Score = 29.1 bits (62), Expect = 2.8
Identities = 23/59 (38%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = -3
Query: 395 TPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPR--CSRPPF*QRFLPGRNAGRPG 225
T I+ YS A I N A L P+ PR S PPF LPG+NAG G
Sbjct: 14 TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADG 72
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 28.7 bits (61), Expect = 3.7
Identities = 17/54 (31%), Positives = 23/54 (42%)
Frame = +2
Query: 251 EEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSD 412
+EI D Y EG EE+ ++ R + L E C E+VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101
>At3g49540.1 68416.m05414 expressed protein
Length = 166
Score = 28.3 bits (60), Expect = 4.9
Identities = 17/49 (34%), Positives = 28/49 (57%)
Frame = +2
Query: 368 EGCTAESVESRLSSDGVLSVIAPRKVPPAVEGERKIPIAQTGPVRKEVK 514
E E+ E+++ + ++V AP KV AVE E+K A+ PV+ E +
Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKK---AEAEPVKAEAE 142
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 27.5 bits (58), Expect = 8.6
Identities = 15/48 (31%), Positives = 20/48 (41%)
Frame = -3
Query: 416 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPRCSRP 273
HHL + ++ P S P S + H SS PRCS+P
Sbjct: 26 HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73
>At1g34180.1 68414.m04239 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
similar to NAM-like protein GI:8809651 from (Arabidopsis
thaliana)
Length = 564
Score = 27.5 bits (58), Expect = 8.6
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = +2
Query: 290 VEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSDGVLSVIAPR 439
+E E D G RQF +P+G +AE ++S L ++ I PR
Sbjct: 229 IEKLLNEIPDAPGVNQRQFDEFVGVPQGNSAEVIQSTLLNNSSGEYIDPR 278
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,199,039
Number of Sequences: 28952
Number of extensions: 344201
Number of successful extensions: 958
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 916
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 957
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1428369392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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