BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0531 (732 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC8E11.04c |||phospholipase |Schizosaccharomyces pombe|chr 1||... 152 6e-38 SPAC9G1.08c |||phospholipase |Schizosaccharomyces pombe|chr 1|||... 32 0.097 SPAC607.06c |||metallopeptidase|Schizosaccharomyces pombe|chr 1|... 30 0.39 SPAC13G6.06c |||glycine cleavage complex subunit P|Schizosacchar... 27 3.6 SPBC17D1.03c |||exosome subunit Rrp43 |Schizosaccharomyces pombe... 27 3.6 SPAC25B8.03 |||phosphatidylserine decarboxylase|Schizosaccharomy... 26 6.4 SPAC6G10.03c |||abhydrolase family protein, unknown biological r... 26 6.4 >SPAC8E11.04c |||phospholipase |Schizosaccharomyces pombe|chr 1|||Manual Length = 224 Score = 152 bits (368), Expect = 6e-38 Identities = 83/221 (37%), Positives = 127/221 (57%), Gaps = 8/221 (3%) Frame = +3 Query: 6 NPVIIAAQARHTASLIFLHGLGDTGHGW---ASTIAGIRGPHVKVICPTASTMPVTLNNG 176 N VII HTA++IFLHGLGD+G GW A+T + + H+K I P A ++PVT+NNG Sbjct: 6 NSVIINPSVAHTATVIFLHGLGDSGQGWSFMANTWSNFK--HIKWIFPNAPSIPVTVNNG 63 Query: 177 FRMPSWFDLRTLDATAPEDEEGIERATDLVHGLIADEVKAGVPADKVLLGGFSQGGXXXX 356 +MP+W+D+ + EDE GI R+ +H LI E+ G+P+D++L+GGFSQG Sbjct: 64 MKMPAWYDIYSFADMKREDENGILRSAGQLHELIDAELALGIPSDRILIGGFSQGCMVSL 123 Query: 357 XXXXTYPERLAGVMSLSCWLPRHGYFPGGL-KAPVDLPIFQAHGDKDPVVSFKWGQMTAS 533 TYP+RLAG+M S +LP FP L + ++PI + +DP+V ++++ Sbjct: 124 YAGLTYPKRLAGIMGHSGFLPLASKFPSALSRVAKEIPILLTYMTEDPIVP---SVLSSA 180 Query: 534 CLKTFMKNVKFS----TYQGLAHSSSIAELKDMQEFIEKTL 644 K + N++ ++G AHS S M +F + + Sbjct: 181 SAKYLINNLQLKCLDRPFEGDAHSLSSESFMAMYKFTQTVI 221 >SPAC9G1.08c |||phospholipase |Schizosaccharomyces pombe|chr 1|||Manual Length = 241 Score = 31.9 bits (69), Expect = 0.097 Identities = 21/100 (21%), Positives = 42/100 (42%), Gaps = 2/100 (2%) Frame = +3 Query: 48 LIFLHGLGDTGHGWASTIAGIRGPHVKVICPTAS-TMPVTLNNGFRMPSWFDLRTLDATA 224 +I +HGLGD+ +A+ + P+ I +P+ N W + D Sbjct: 27 VILMHGLGDSHKSFANMAKNVPLPNTSYISLRGPYRLPLDFENPGGNWMWGEDVHFDQNG 86 Query: 225 P-EDEEGIERATDLVHGLIADEVKAGVPADKVLLGGFSQG 341 + E ++ ++ LI + + G+ + ++ GF QG Sbjct: 87 ELQSEADFSKSFTMISNLIGNLLSYGILSSRIFFFGFGQG 126 >SPAC607.06c |||metallopeptidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 612 Score = 29.9 bits (64), Expect = 0.39 Identities = 12/20 (60%), Positives = 13/20 (65%) Frame = -3 Query: 589 CASPWYVENLTFFINVLRHD 530 C SPW E LT IN+LR D Sbjct: 165 CLSPWGTERLTATINILRSD 184 >SPAC13G6.06c |||glycine cleavage complex subunit P|Schizosaccharomyces pombe|chr 1|||Manual Length = 1017 Score = 26.6 bits (56), Expect = 3.6 Identities = 13/44 (29%), Positives = 23/44 (52%) Frame = +1 Query: 490 RTQLYLSNGDK*RHHASRHL*KMLNFQHTKDSHIAHPLPNSKTC 621 RT LYL + R+H+ L + ++ +KD +AH + +C Sbjct: 530 RTTLYLQHPVFNRYHSETELMRYIHHLQSKDLSLAHAMTPLGSC 573 >SPBC17D1.03c |||exosome subunit Rrp43 |Schizosaccharomyces pombe|chr 2|||Manual Length = 270 Score = 26.6 bits (56), Expect = 3.6 Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 2/40 (5%) Frame = +3 Query: 126 KVICPTASTMPVTLNNGFRMPSW--FDLRTLDATAPEDEE 239 +VIC + T PV L+ R SW FD + L E+E+ Sbjct: 188 RVICASTLTRPVQLSTEVRSFSWSVFDDKLLADPTDEEED 227 >SPAC25B8.03 |||phosphatidylserine decarboxylase|Schizosaccharomyces pombe|chr 1|||Manual Length = 516 Score = 25.8 bits (54), Expect = 6.4 Identities = 12/35 (34%), Positives = 18/35 (51%) Frame = +3 Query: 513 WGQMTASCLKTFMKNVKFSTYQGLAHSSSIAELKD 617 WGQ + L TF++ F Y +++ELKD Sbjct: 91 WGQFNRAHLPTFLRTPGFKLY-AWVFGCNLSELKD 124 >SPAC6G10.03c |||abhydrolase family protein, unknown biological role|Schizosaccharomyces pombe|chr 1|||Manual Length = 428 Score = 25.8 bits (54), Expect = 6.4 Identities = 24/81 (29%), Positives = 36/81 (44%) Frame = +3 Query: 165 LNNGFRMPSWFDLRTLDATAPEDEEGIERATDLVHGLIADEVKAGVPADKVLLGGFSQGG 344 + N R P FD++ TA E E ER L + G+ +K++L G S GG Sbjct: 132 MGNSSRPP--FDIK--GQTASEKVEETERF--FTESLETWRIGHGI--EKMILVGHSMGG 183 Query: 345 XXXXXXXXTYPERLAGVMSLS 407 YPER+ ++ +S Sbjct: 184 YLSAVYAMQYPERVEKLLLVS 204 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,084,724 Number of Sequences: 5004 Number of extensions: 65517 Number of successful extensions: 163 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 156 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 162 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 345237368 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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