SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0528
         (570 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC2F12.15c |||palmitoyltransferase|Schizosaccharomyces pombe|c...    29   0.36 
SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizos...    27   2.6  
SPBC21D10.05c |ucp3|soc2|GTPase activating protein Ucp3 |Schizos...    26   3.4  
SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizos...    26   3.4  
SPBPB2B2.18 |||dubious|Schizosaccharomyces pombe|chr 2|||Manual        26   4.5  
SPCC1393.14 |||nuclear telomere cap complex subunit Ten1|Schizos...    25   5.9  
SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|...    25   5.9  
SPBC23G7.11 |||DNA-3-methyladenine glycosidase Mag2 |Schizosacch...    25   7.8  
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce...    25   7.8  
SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces po...    25   7.8  

>SPBC2F12.15c |||palmitoyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 329

 Score = 29.5 bits (63), Expect = 0.36
 Identities = 15/47 (31%), Positives = 20/47 (42%), Gaps = 2/47 (4%)
 Frame = -3

Query: 301 RLCER--DYRCQRIHQPMVCCYCCTN*HNCQ*NSPSHCSWLETCYHF 167
           R CE+  +Y+C R H    C  C    + C      HC W + C  F
Sbjct: 97  RFCEKCQEYKCDRSHH---CSQC----NKCILRMDHHCMWFKNCVGF 136


>SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase
           Ppk30|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 953

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 13/31 (41%), Positives = 16/31 (51%)
 Frame = +2

Query: 182 LQPATMRWRILLTIVSVCTAIAAYHWLMDPL 274
           LQ       IL  +  VC A+AA H+L  PL
Sbjct: 153 LQHRLTEGEILKILADVCDAVAAMHYLDPPL 183


>SPBC21D10.05c |ucp3|soc2|GTPase activating protein Ucp3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 601

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = +1

Query: 178 MSPTSYNEMENSTDNCVSLYSNSSIPLVDGSS 273
           MS  S NE   +  N  S Y N+S+P +D ++
Sbjct: 417 MSKLSLNEQPAAPSNSNSQYMNTSMPTLDSTN 448


>SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 732

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 15/47 (31%), Positives = 23/47 (48%)
 Frame = +1

Query: 181 SPTSYNEMENSTDNCVSLYSNSSIPLVDGSSDTCSLAHTVFVESSLF 321
           S +S  ++   T + +S  S S  P  DGS D+  +AH  +  S  F
Sbjct: 241 SHSSIGDLTTITQSSISSGSGSFKPSWDGSFDSSLMAHQSYGTSPAF 287


>SPBPB2B2.18 |||dubious|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 175

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 14/44 (31%), Positives = 19/44 (43%)
 Frame = +2

Query: 152 ERRLTEVIACLQPATMRWRILLTIVSVCTAIAAYHWLMDPLTPV 283
           +RRL   I         W  LL +V VC  +     L+ P+ PV
Sbjct: 10  QRRLVREINKFSSFESNWLFLLFLVRVCRQLKTVTVLIVPVLPV 53


>SPCC1393.14 |||nuclear telomere cap complex subunit
           Ten1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 102

 Score = 25.4 bits (53), Expect = 5.9
 Identities = 11/23 (47%), Positives = 14/23 (60%), Gaps = 1/23 (4%)
 Frame = +1

Query: 223 CVSLYSNSSIPLVDGSSD-TCSL 288
           CV  Y N  + L+DGSS  TC +
Sbjct: 26  CVQSYKNGILRLIDGSSSVTCDV 48


>SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit
           Alp4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 784

 Score = 25.4 bits (53), Expect = 5.9
 Identities = 12/33 (36%), Positives = 21/33 (63%)
 Frame = -3

Query: 172 HFS*TTLKSFQIITSSLFERHYLILKDKFVNVE 74
           +FS  TL+ + + TS    + YLIL+D  +++E
Sbjct: 180 NFSLQTLRLYTLPTSRSLRQVYLILRDLLLSME 212


>SPBC23G7.11 |||DNA-3-methyladenine glycosidase Mag2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 213

 Score = 25.0 bits (52), Expect = 7.8
 Identities = 10/29 (34%), Positives = 15/29 (51%)
 Frame = +2

Query: 362 GVHRKVVAPSIITARTRSILNDFNMSCDD 448
           G+ R + +  +  A T SI+N F   C D
Sbjct: 44  GIIRAITSQKLSDAATNSIINKFCTQCSD 72


>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1036

 Score = 25.0 bits (52), Expect = 7.8
 Identities = 16/48 (33%), Positives = 25/48 (52%)
 Frame = +1

Query: 175 SMSPTSYNEMENSTDNCVSLYSNSSIPLVDGSSDTCSLAHTVFVESSL 318
           S S TS + + +S+ N  +  S +S  L   S+ + SLA +    SSL
Sbjct: 82  SSSLTSSSSLASSSTNSTTSASPTSSSLTSSSATSSSLASSSTTSSSL 129


>SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1564

 Score = 25.0 bits (52), Expect = 7.8
 Identities = 13/26 (50%), Positives = 15/26 (57%)
 Frame = +1

Query: 190  SYNEMENSTDNCVSLYSNSSIPLVDG 267
            SY     S DNC+ L+S S   LVDG
Sbjct: 1387 SYINDSFSVDNCLRLFSWSHSLLVDG 1412


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,081,508
Number of Sequences: 5004
Number of extensions: 39851
Number of successful extensions: 116
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 116
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 242064240
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -