Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0527
(699 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g01280.1 68416.m00035 porin, putative similar to SP|P42055 34... 64 1e-10
At5g57490.1 68418.m07184 porin, putative similar to 36kDA porin ... 60 1e-09
At5g67500.1 68418.m08512 porin, putative similar to SP|P42055 34... 54 7e-08
At3g49920.1 68416.m05458 porin, putative similar to SP|P42055 34... 32 0.42
At2g36340.1 68415.m04460 DNA-binding storekeeper protein-related... 29 2.2
At3g13610.1 68416.m01713 oxidoreductase, 2OG-Fe(II) oxygenase fa... 29 3.0
At3g17420.1 68416.m02225 protein kinase family protein contains ... 29 3.9
At4g34040.1 68417.m04830 zinc finger (C3HC4-type RING finger) fa... 28 6.8
At4g30810.1 68417.m04365 serine carboxypeptidase S10 family prot... 28 6.8
At3g19840.1 68416.m02513 FF domain-containing protein / WW domai... 28 6.8
At4g25920.1 68417.m03727 expressed protein contains Pfam profile... 27 9.0
At2g04740.1 68415.m00484 ankyrin repeat family protein contains ... 27 9.0
>At3g01280.1 68416.m00035 porin, putative similar to SP|P42055 34
kDa outer mitochondrial membrane protein porin
(Voltage-dependent anion-selective channel protein)
(VDAC) {Solanum tuberosum}; contains Pfam profile
PF01459: Eukaryotic porin
Length = 276
Score = 63.7 bits (148), Expect = 1e-10
Identities = 51/199 (25%), Positives = 88/199 (44%)
Frame = +1
Query: 85 PPYYADLGKKANDVFSKGYHFGVFKLDLKTKSESGVEFTSGITSNQESGKVFGSLSSKFA 264
P Y ++GKKA D+ K H K + T S +GV TS T ++ + G ++ F
Sbjct: 5 PGLYTEIGKKARDLLYKD-HNSDQKFSITTFSPAGVAITS--TGTKKGDLLLGDVA--FQ 59
Query: 265 VKDYGLTFTEKWNTDNTLATDITIQDKIAAGLKVTLEGTFAPQTGTKTGKLKTSFTNDTV 444
+ +T K TD+T T+ D+ A GL+ Q +GK++ + ++
Sbjct: 60 SRRKNITTDLKVCTDSTFLITATV-DEAAPGLRSIFSFKVPDQN---SGKVELQYLHEYA 115
Query: 445 AVNTNLDLDLAGPVVDVAAVLNYQGWLAGVHTQFDTQKAKFSKNNFALSYQSGDFALHTN 624
++T++ L P V+ + V+ G FDT+ F+K N LS+ D
Sbjct: 116 GISTSMGLT-QNPTVNFSGVIGSNVLAVGTDVSFDTKSGNFTKINAGLSFTKEDLIASLT 174
Query: 625 VDNGKDFGGSIYQKVSDKL 681
V++ D + Y + + L
Sbjct: 175 VNDKGDLLNASYYHIVNPL 193
>At5g57490.1 68418.m07184 porin, putative similar to 36kDA porin II
[Solanum tuberosum] GI:515360; contains Pfam profile
PF01459: Eukaryotic porin
Length = 274
Score = 60.1 bits (139), Expect = 1e-09
Identities = 48/207 (23%), Positives = 95/207 (45%), Gaps = 1/207 (0%)
Frame = +1
Query: 82 APPYYADLGKKANDVFSKGYHFGVFKLDLKTKSESGVEFTSGITSNQESGKVFGSLSSKF 261
+P +AD+GKKA D+ +K Y F K L S +G EF + T ++ FG +S+ +
Sbjct: 4 SPAPFADIGKKAKDLLNKDYIFD-HKFTLTMLSATGTEFVA--TGLKKDDFFFGDISTLY 60
Query: 262 AVKDYGLTFTEKWNTDNTLATDITIQDKIAAGLKVTLEGTFAPQTGTKTGKLKTSFTNDT 441
K K ++ ++++T +T+++ + + K + K+GKL + +
Sbjct: 61 --KGQNTIVDLKIDSHSSVSTKVTLKNLLPSA-KAVISFKIPDH---KSGKLDVQYVHPH 114
Query: 442 VAVNTNLDLDLAGPVVDVAAVLNYQGWLAGVHTQFDTQKAKFSKNNFALSYQS-GDFALH 618
+N+++ L+ P++D++A + Q G FDT + +K N + + + G A
Sbjct: 115 ATLNSSIGLN-PTPLLDLSATIGSQNVCLGGEVSFDTASSSLTKYNAGIGFNNQGVSAAL 173
Query: 619 TNVDNGKDFGGSIYQKVSDKLDCGVSM 699
D G+ + V+ G +
Sbjct: 174 ILEDKGESLRATYVHTVNPTTSFGAEL 200
>At5g67500.1 68418.m08512 porin, putative similar to SP|P42055 34
kDa outer mitochondrial membrane protein porin
(Voltage-dependent anion-selective channel protein)
(VDAC) {Solanum tuberosum}; contains Pfam profile
PF01459: Eukaryotic porin
Length = 276
Score = 54.4 bits (125), Expect = 7e-08
Identities = 46/177 (25%), Positives = 80/177 (45%)
Frame = +1
Query: 85 PPYYADLGKKANDVFSKGYHFGVFKLDLKTKSESGVEFTSGITSNQESGKVFGSLSSKFA 264
P + D+GKKA D+ ++ Y+ K + T S SGV TS T+ ++ G +++++
Sbjct: 5 PGLFTDIGKKAKDLLTRDYNSDQ-KFSISTYSASGVALTS--TALKKGGVHAADVATQYK 61
Query: 265 VKDYGLTFTEKWNTDNTLATDITIQDKIAAGLKVTLEGTFAPQTGTKTGKLKTSFTNDTV 444
K+ F K +TD+++ T +T+ + I K + P + KL+ + +D
Sbjct: 62 YKN--ALFDVKIDTDSSVLTTVTLTE-ILPSTKA-IASFKVPDYNS--AKLEVQYFHDHA 115
Query: 445 AVNTNLDLDLAGPVVDVAAVLNYQGWLAGVHTQFDTQKAKFSKNNFALSYQSGDFAL 615
V L P++D+ A L G +DT F+K N +S D L
Sbjct: 116 TVTAAAALK-QNPLIDITATLGSPVISFGAEAGYDTTSKTFTKYNAGISVTKPDACL 171
>At3g49920.1 68416.m05458 porin, putative similar to SP|P42055 34
kDa outer mitochondrial membrane protein porin
(Voltage-dependent anion-selective channel protein)
(VDAC) {Solanum tuberosum}; contains Pfam profile
PF01459: Eukaryotic porin
Length = 226
Score = 31.9 bits (69), Expect = 0.42
Identities = 17/45 (37%), Positives = 23/45 (51%)
Frame = +1
Query: 85 PPYYADLGKKANDVFSKGYHFGVFKLDLKTKSESGVEFTSGITSN 219
P +AD+GK A D+ ++ Y K + T S SGV TS N
Sbjct: 5 PGLFADIGKYAKDLLTRDYSTDQ-KFSISTNSVSGVALTSTALKN 48
>At2g36340.1 68415.m04460 DNA-binding storekeeper protein-related
contains similarity to storekeeper protein [Solanum
tuberosum] gi|14268476|emb|CAC39398; contains PF04504:
Protein of unknown function, DUF573
Length = 414
Score = 29.5 bits (63), Expect = 2.2
Identities = 26/94 (27%), Positives = 43/94 (45%), Gaps = 3/94 (3%)
Frame = +1
Query: 301 NTDNTLATDITIQDKIAAGLKVTLEGTFAPQTGTKTGKLKTSFTNDTVAVNTNLDLDLAG 480
N+D+TL + ++ + + L T T T+T L S + + + N +L + G
Sbjct: 14 NSDDTLTRNREVEVEAMLSRRKQLRTTTTRTTTTRTTPLSLSSSASKMNWSKNDELVILG 73
Query: 481 PVVDV--AAVLNYQG-WLAGVHTQFDTQKAKFSK 573
+VD L+Y+ W A D +AKFSK
Sbjct: 74 GIVDYENETKLSYRSDWDALYRYIKDCVEAKFSK 107
>At3g13610.1 68416.m01713 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to desacetoxyvindoline
4-hydroxylase [Catharanthus roseus][GI:1916643],
flavonol synthase 1 [SP|Q96330]; contains PF03171
2OG-Fe(II) oxygenase superfamily domain
Length = 361
Score = 29.1 bits (62), Expect = 3.0
Identities = 18/59 (30%), Positives = 27/59 (45%), Gaps = 1/59 (1%)
Frame = -2
Query: 356 PAAILSWIVMSVANVLSVFHFSVKVKP*SFTANLEERLPKTFPLSWLEVIPLVN-STPD 183
PA + ++V V + +K P + LEERL F E IP+++ S PD
Sbjct: 13 PAEVTDFVVYKGNGVKGLSETGIKALPEQYIQPLEERLINKFVNETDEAIPVIDMSNPD 71
>At3g17420.1 68416.m02225 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; contains
serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 467
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +1
Query: 568 SKNNFALSYQSGDFALHTNVDNGKDFGGSIYQK 666
S NN SYQ+ D +++NG F GS+ +K
Sbjct: 75 SSNNNGTSYQTLDEKFVEDIENGDKFSGSLEKK 107
>At4g34040.1 68417.m04830 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 666
Score = 27.9 bits (59), Expect = 6.8
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = -2
Query: 92 YGGAMSVFYKFTNWYYFTENSTQIGARDHL 3
Y + S + NW+ F E+S+++G DHL
Sbjct: 54 YASSSSHVVQNHNWWSFGESSSRLGPSDHL 83
>At4g30810.1 68417.m04365 serine carboxypeptidase S10 family protein
similar to serine-type carboxypeptidase (SP:P55748)
[Hordeum vulgare]
Length = 479
Score = 27.9 bits (59), Expect = 6.8
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = -1
Query: 285 SQAIVFHCKFGGKAAKNLSAFLVGGDSAGEFNTRLAL 175
S+AIV H + K + NL ++VG +F+ RL L
Sbjct: 196 SEAIVKHNQGSDKNSINLKGYMVGNGLMDDFHDRLGL 232
>At3g19840.1 68416.m02513 FF domain-containing protein / WW
domain-containing protein weak similarity to
transcription factor CA150b [Mus musculus] GI:6329166;
contains Pfam profiles PF01846: FF domain, PF00397: WW
domain
Length = 743
Score = 27.9 bits (59), Expect = 6.8
Identities = 20/56 (35%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Frame = +1
Query: 73 TDMAPPYYADLGKKANDVFSKGYHFGVFKLDLKTKS--ESGVEFTSGITSNQESGK 234
T ++ P ++ G+ A + K +FG LDL K +SG+ +S ITS SGK
Sbjct: 268 TSLSAPAISNGGRDAASL--KTTNFGSSALDLVKKKLHDSGMPVSSTITSEANSGK 321
>At4g25920.1 68417.m03727 expressed protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 390
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 88 PYYADLGKKANDVFSKGYHFGVFKLDLKT 174
P + G K N ++ GY+FGV+ L KT
Sbjct: 330 PASSSPGLKPNCIYFVGYNFGVYDLTTKT 358
>At2g04740.1 68415.m00484 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 578
Score = 27.5 bits (58), Expect = 9.0
Identities = 16/55 (29%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
Frame = -2
Query: 413 FPVLVPVCGAKVPSRVTLRPAAILSWIVMS-VANVLSVFHFSVKVKP*SFTANLE 252
FP+ V A +P T PA + W+V+S + VL + + + + +F A +E
Sbjct: 431 FPLKRAVADALLPHLETATPAELCQWLVLSDMYGVLKIREYCLDLVACNFEAFVE 485
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,010,244
Number of Sequences: 28952
Number of extensions: 309800
Number of successful extensions: 877
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 850
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 875
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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