BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0523 (739 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 32 0.098 SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy... 31 0.13 SPCC569.01c |||DUF1773 family protein 5|Schizosaccharomyces pomb... 31 0.23 SPCP20C8.01c |||B13958 domain|Schizosaccharomyces pombe|chr 3|||... 31 0.23 SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66 |... 28 1.6 SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 27 3.7 SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce... 27 3.7 SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 27 3.7 SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 4.9 SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|ch... 26 4.9 SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch... 26 4.9 SPBC13E7.08c |||RNA polymerase II associated Paf1 complex |Schiz... 26 6.4 >SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces pombe|chr 2|||Manual Length = 2493 Score = 31.9 bits (69), Expect = 0.098 Identities = 27/98 (27%), Positives = 47/98 (47%) Frame = +1 Query: 4 SSYDSYNDRLGEILNRSITSKMRDSSIDETLAGFGKLRISALTKAVELSPLVKEATIGPN 183 SSY S DRL L+R +TS +R +SID +A L+ + + +++ L + E P+ Sbjct: 568 SSYTSLMDRLINNLSRGLTS-LRAASIDLIIALCKSLQKNEVLQSLSLVKKLSELPFDPS 626 Query: 184 SPAKKELIYEEKQIPAKIEPLPEDLVEDKLRGDLGYNL 297 + ++ + + AK L +D L LG + Sbjct: 627 TSRDASVLL--RNLSAKSSALEKDTRRVVLHALLGLTI 662 >SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 31.5 bits (68), Expect = 0.13 Identities = 47/190 (24%), Positives = 89/190 (46%), Gaps = 9/190 (4%) Frame = +1 Query: 49 RSITSKMRDSSIDETLAGFGKLRISALTKA-VELSPLVKEATIGPNSPAKKELIYEEKQI 225 R I SK RD D+ L F + A TK +K+ +G + A L++ K Sbjct: 405 RKIVSKDRDL-YDKGLRAFVS-HVRAYTKHHASFIFRIKDLDLGQLATAYA-LLHLPKMP 461 Query: 226 PAKIEPLPEDLVE--DKLRGDLGYNLIMKECEAQLHQLSVEFFRKSFEQMICKRAEIMNK 399 K + E++ + D + Y ++E +A+ +L VE K+ + + + A+I N+ Sbjct: 462 ELKDTEISENIFKKFDIDYATIPYRDQVRE-QARRRRLEVE---KTEPKKLARPAKIKNE 517 Query: 400 CFQKYSEQRERRDMKYHKRK-----LQLQKDFHEND-NLNVLKIAKLDEQNNSAMINRQT 561 + K E +E+R+ + KRK L+ QK+ N+ ++ A E N +I+ ++ Sbjct: 518 AWSKQKEVKEKRNTRREKRKSKKEFLKAQKNEASNNLKQEIVSKAGAQETENDDLIDEES 577 Query: 562 IDNMNRILEE 591 D ++ + E+ Sbjct: 578 -DALSELEED 586 >SPCC569.01c |||DUF1773 family protein 5|Schizosaccharomyces pombe|chr 3|||Manual Length = 323 Score = 30.7 bits (66), Expect = 0.23 Identities = 32/144 (22%), Positives = 68/144 (47%), Gaps = 12/144 (8%) Frame = +1 Query: 280 DLGYNLIMKECEAQLHQLSVEFFRKSFEQMICKRAEIMNKCFQKYSEQRERRDMKYH--- 450 D YNL A+ ++ ++ + +S + + + E + +KY E+R+R+ MKY Sbjct: 8 DNDYNLPPPNDSAEDLKIFIKRYERSVDSTLLEIDENKREALEKYIEERDRK-MKYEIEC 66 Query: 451 ------KRKLQLQKDFHENDNLNVLKIAKLDEQNNSAM--INRQTIDNMNRILEEQNKAT 606 +KL ++++ E + V +DE N+ + I + M+ + QN Sbjct: 67 NERLQGWKKLAIEREISEEQSGEVQFPRWIDEWANTKLGGIFERIFSKMDSM---QNDMN 123 Query: 607 ARFAAI-TDSHTKICICYNEITNV 675 +RF A+ + +++ NE+T++ Sbjct: 124 SRFDAMQNEMNSRFDAVQNEMTSM 147 >SPCP20C8.01c |||B13958 domain|Schizosaccharomyces pombe|chr 3|||Manual Length = 247 Score = 30.7 bits (66), Expect = 0.23 Identities = 32/144 (22%), Positives = 68/144 (47%), Gaps = 12/144 (8%) Frame = +1 Query: 280 DLGYNLIMKECEAQLHQLSVEFFRKSFEQMICKRAEIMNKCFQKYSEQRERRDMKYH--- 450 D YNL A+ ++ ++ + +S + + + E + +KY E+R+R+ MKY Sbjct: 8 DNDYNLPPPNDSAEDLKIFIKRYERSVDSTLLEIDENKREALEKYIEERDRK-MKYEIEC 66 Query: 451 ------KRKLQLQKDFHENDNLNVLKIAKLDEQNNSAM--INRQTIDNMNRILEEQNKAT 606 +KL ++++ E + V +DE N+ + I + M+ + QN Sbjct: 67 NERLQGWKKLAIEREISEEQSGEVQFPRWIDEWANTKLGGIFERIFSKMDSM---QNDMN 123 Query: 607 ARFAAI-TDSHTKICICYNEITNV 675 +RF A+ + +++ NE+T++ Sbjct: 124 SRFDAMQNEMNSRFDTVQNEMTSM 147 >SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66 |Schizosaccharomyces pombe|chr 1|||Manual Length = 649 Score = 27.9 bits (59), Expect = 1.6 Identities = 21/86 (24%), Positives = 40/86 (46%), Gaps = 1/86 (1%) Frame = +1 Query: 181 NSPAKKELIYEE-KQIPAKIEPLPEDLVEDKLRGDLGYNLIMKECEAQLHQLSVEFFRKS 357 NS A + + EE +I + P D+ E+K + +L + Q Q + ++ Sbjct: 4 NSGASESISIEETNRIRISLGLKPLDISEEKPQKELSDASVKSSYVDQEQQAYENWKKQE 63 Query: 358 FEQMICKRAEIMNKCFQKYSEQRERR 435 E++ K+ E + F+K ++ ERR Sbjct: 64 QEEINRKKEEELKSKFEKLRQKNERR 89 >SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1727 Score = 26.6 bits (56), Expect = 3.7 Identities = 51/253 (20%), Positives = 104/253 (41%), Gaps = 13/253 (5%) Frame = +1 Query: 16 SYNDRLGEILNRSI--TSKMRDSSID-ETLAGFGKLRISALTKAVELSPLVKEATIGPNS 186 S N L +I++R SK + + +LA K ++A +L +E I N Sbjct: 717 SINQNLQDIISRQDQRASKFAEELLHVNSLAERLKGELNASKGEKDLRKRTQERLISEND 776 Query: 187 PAKKELIYEEKQIPAKIEPLPEDLVEDKLRGDLGYNLIMKECEAQLHQLSVEFFRKSFEQ 366 +L+ E +++ + + L L + +L + E E + LS++ ++S E+ Sbjct: 777 ----KLLAERERLMSLVSDLQTFLNQQQLSD--AARKVKFESEKESLSLSLQKLKESNEK 830 Query: 367 MICKRAEIMNKCFQKYSEQRERRDMKYHKRKLQLQKDFHEN-DNLNVLKIAKLDEQNNSA 543 M + K +K + R K L +D + DN +++I KL E N Sbjct: 831 M-SNDLHSLQKSLEKSGIEYSSRIKTLMLEKQSLSEDNRKLLDNQQMMEI-KLQELNGVI 888 Query: 544 MINRQTIDNMN-RILEEQNKATARFAAITDS--------HTKICICYNEITNVIASEPLS 696 + +Q + + +++N + + A+ +S HT + YN I + Sbjct: 889 ELEKQRFSTLEAKFTQQKNTSYSEREALLESSLSDLQSKHTSLESQYNYSLRNIEQLQAA 948 Query: 697 QQVCDKYIPTIKT 735 ++ ++ + +KT Sbjct: 949 SKLAEEMVERVKT 961 >SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1210 Score = 26.6 bits (56), Expect = 3.7 Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 3/63 (4%) Frame = +1 Query: 1 DSSYDSYNDRLGEILNRSITSKMRDSSID---ETLAGFGKLRISALTKAVELSPLVKEAT 171 ++ +++ N +G + SI S MR D E L GF L S ++V LSP+ Sbjct: 140 ENIFNAPNIIIGPRSDFSINSVMRSILCDITAELLEGFSSLEFSIHARSVILSPITDMPH 199 Query: 172 IGP 180 + P Sbjct: 200 LAP 202 >SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1019 Score = 26.6 bits (56), Expect = 3.7 Identities = 29/121 (23%), Positives = 50/121 (41%) Frame = +1 Query: 340 EFFRKSFEQMICKRAEIMNKCFQKYSEQRERRDMKYHKRKLQLQKDFHENDNLNVLKIAK 519 EF KSF + + KRA +M K Q+ + E + +K +Q +K+ E+ A+ Sbjct: 404 EFNIKSFNRNLVKRAAVMAK-LQRNQLEEELKAKGIYKPTIQGEKEEEEDPLERARNDAE 462 Query: 520 LDEQNNSAMINRQTIDNMNRILEEQNKATARFAAITDSHTKICICYNEITNVIASEPLSQ 699 Q A N +N EE+ +++ + T I + I +EP + Sbjct: 463 KIRQLEKASGNASDEGELND--EEEVISSSNTPSTKAKTTNKVIISDVIIEATQAEPKRR 520 Query: 700 Q 702 Q Sbjct: 521 Q 521 >SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 775 Score = 26.2 bits (55), Expect = 4.9 Identities = 18/58 (31%), Positives = 30/58 (51%), Gaps = 2/58 (3%) Frame = +1 Query: 451 KRKLQLQKDFHENDNLNVLKIAKL-DEQNNSAMINRQTIDN-MNRILEEQNKATARFA 618 K + + K EN+NL V KIA++ +E + IN Q N ++ EE +++ A Sbjct: 107 KNLISISKKLSENENLPVSKIAEVTNELDTLVRINDQLSRNHISGTTEEMQSSSSLIA 164 >SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 775 Score = 26.2 bits (55), Expect = 4.9 Identities = 12/24 (50%), Positives = 18/24 (75%) Frame = -3 Query: 137 ALVRADILNLPNPAKVSSILESLI 66 ALV+ D+ L N +K+S++LES I Sbjct: 536 ALVKDDVETLCNSSKISALLESSI 559 >SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1208 Score = 26.2 bits (55), Expect = 4.9 Identities = 30/123 (24%), Positives = 51/123 (41%) Frame = +1 Query: 232 KIEPLPEDLVEDKLRGDLGYNLIMKECEAQLHQLSVEFFRKSFEQMICKRAEIMNKCFQK 411 +I+ L DL ++K N IM E EA + L+ + ++ + K E + + Sbjct: 360 RIQVLTADLEKEK------ENQIMHESEASIG-LTDSMQVHTLQEQLHKANEEIEFLHDQ 412 Query: 412 YSEQRERRDMKYHKRKLQLQKDFHENDNLNVLKIAKLDEQNNSAMINRQTIDNMNRILEE 591 S E + LQ + E D L K+ L++ NNS + ++ N L Sbjct: 413 ISRMNEE-GKNFEDIMLQFRSLEEERDVLES-KLQTLEDDNNSLRLMTSSLGNQIESLRT 470 Query: 592 QNK 600 QN+ Sbjct: 471 QNR 473 >SPBC13E7.08c |||RNA polymerase II associated Paf1 complex |Schizosaccharomyces pombe|chr 2|||Manual Length = 429 Score = 25.8 bits (54), Expect = 6.4 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = +1 Query: 361 EQMICKRAEIMNKCFQKYSEQRERRDMKYHKRK 459 E +I K E++ + +KY E+R R + KRK Sbjct: 236 EIIIEKDPELLKRQAEKYEEERSRARRRLEKRK 268 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.316 0.131 0.363 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,873,894 Number of Sequences: 5004 Number of extensions: 58964 Number of successful extensions: 214 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 205 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 214 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 349251756 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits)
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