Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0523
(739 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g27595.1 68417.m03964 protein transport protein-related low s... 35 0.049
At2g33793.1 68415.m04145 expressed protein 35 0.065
At5g42710.1 68418.m05202 hypothetical protein 32 0.35
At5g48610.1 68418.m06012 expressed protein ; expression supporte... 31 1.1
At1g51900.1 68414.m05850 hypothetical protein 31 1.1
At5g15920.1 68418.m01862 structural maintenance of chromosomes (... 30 1.4
At1g08730.1 68414.m00969 myosin heavy chain (PCR43) identical to... 29 2.4
At1g48360.1 68414.m05402 expressed protein ; expression supporte... 29 4.3
At4g36270.1 68417.m05158 ATP-binding region, ATPase-like domain-... 28 5.6
At2g35330.1 68415.m04332 zinc finger (C3HC4-type RING finger) pr... 28 5.6
At2g26870.1 68415.m03224 phosphoesterase family protein low simi... 28 5.6
At1g64820.1 68414.m07349 MATE efflux family protein similar to r... 28 5.6
At5g47240.1 68418.m05825 MutT/nudix family protein similar to SP... 28 7.4
At3g46200.1 68416.m05001 MutT/nudix family protein similar to he... 28 7.4
At1g68790.1 68414.m07863 expressed protein 28 7.4
At5g18770.1 68418.m02230 F-box family protein contains F-box dom... 27 9.8
At3g05740.1 68416.m00644 DNA helicase (RECQI1) identical to DNA ... 27 9.8
At1g16060.2 68414.m01927 ovule development protein, putative sim... 27 9.8
At1g16060.1 68414.m01926 ovule development protein, putative sim... 27 9.8
>At4g27595.1 68417.m03964 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 1212
Score = 35.1 bits (77), Expect = 0.049
Identities = 45/172 (26%), Positives = 75/172 (43%), Gaps = 16/172 (9%)
Frame = +1
Query: 115 RISALTKAVELSPLVKEATIGPNSPAKKELIYEEKQIPAKIEPLP---EDLV--EDKLRG 279
+I L+ A E S + KE + +EL E KIE L E LV E KL+
Sbjct: 683 KIEELSAAKE-SLVEKETKLLSTVQEAEELRRRELACLKKIEELSAVNERLVDKETKLQS 741
Query: 280 DLGYNLIMKECEA----QLHQLSVEFFR-----KSFEQMICKRAEIMNK--CFQKYSEQR 426
+ ++KE EA Q+ +LS+ R + ++ + E+ K +QK E+
Sbjct: 742 SIQEVEVLKEREAENIKQIEELSLSNERLVEKEAKLQTVVQENEELREKESAYQKKIEEL 801
Query: 427 ERRDMKYHKRKLQLQKDFHENDNLNVLKIAKLDEQNNSAMINRQTIDNMNRI 582
+ D + R+ +LQ EN+ L ++A L + A + +D N +
Sbjct: 802 SKVDEIFADREAKLQSSTQENEELREREVAYLKKIEELAKLQENLLDKENEL 853
>At2g33793.1 68415.m04145 expressed protein
Length = 212
Score = 34.7 bits (76), Expect = 0.065
Identities = 23/105 (21%), Positives = 47/105 (44%), Gaps = 2/105 (1%)
Frame = +1
Query: 220 QIPAKIEPLPEDLVEDKLRGDLGYNLIMKECEAQLHQLSVEF--FRKSFEQMICKRAEIM 393
++ +KI+ L L +++ + KECE L + +F K F + + +
Sbjct: 72 EVKSKIDELKSKLEKERQNFSKALSKSSKECENILKDEAAKFEELHKKFVKDKADHLQGL 131
Query: 394 NKCFQKYSEQRERRDMKYHKRKLQLQKDFHENDNLNVLKIAKLDE 528
K+ E +ER M+Y + + + + E + K+A+L+E
Sbjct: 132 KDTISKFEEDKERLYMRYEQLRKKEKTMITEQEKFCTEKLAQLEE 176
>At5g42710.1 68418.m05202 hypothetical protein
Length = 807
Score = 32.3 bits (70), Expect = 0.35
Identities = 30/124 (24%), Positives = 51/124 (41%), Gaps = 6/124 (4%)
Frame = +1
Query: 247 PEDLVEDKLRGDLGYNLIMK---ECEAQLHQLSVEFFRKSFEQMICKRAEIMNKCFQKYS 417
P+D E ++ D G NL + E L +L + S + +I K + N+ K
Sbjct: 303 PQDEKETNIKHD-GENLTRRRVMEASENLVELKTQRKSTSLDLVIHKETQTANEINYKAK 361
Query: 418 EQ---RERRDMKYHKRKLQLQKDFHENDNLNVLKIAKLDEQNNSAMINRQTIDNMNRILE 588
Q RE+ D K KR K E NV+K +N ++ R ++++
Sbjct: 362 SQQKDREKDDSKSRKRSKASYKKDGETTTKNVIKRNPTPTENKHKVVARSQQKPLHKLSN 421
Query: 589 EQNK 600
++ K
Sbjct: 422 KKEK 425
>At5g48610.1 68418.m06012 expressed protein ; expression supported
by MPSS
Length = 470
Score = 30.7 bits (66), Expect = 1.1
Identities = 19/75 (25%), Positives = 33/75 (44%)
Frame = +1
Query: 376 KRAEIMNKCFQKYSEQRERRDMKYHKRKLQLQKDFHENDNLNVLKIAKLDEQNNSAMINR 555
KRAE+M K + + R+ + RK + K D V K D+ N
Sbjct: 182 KRAEVMGKPMENRDQVRQTESAEKSHRKENVTKSEKPRDQEGVKKTEAKDKDRNKEKKEE 241
Query: 556 QTIDNMNRILEEQNK 600
+T +++N+ +E+ K
Sbjct: 242 KT-ESINKTRQEKPK 255
>At1g51900.1 68414.m05850 hypothetical protein
Length = 774
Score = 30.7 bits (66), Expect = 1.1
Identities = 21/97 (21%), Positives = 46/97 (47%), Gaps = 4/97 (4%)
Frame = +1
Query: 250 EDLVEDKLRGDLGYNLIMKECEAQLHQLSV---EFFRKSFEQMICKRAEIMNKCFQKYSE 420
E+++ED R D+ ++ + H++ + E+++ E+ + ++ E Y
Sbjct: 309 EEIMEDDYREDMKCRMLDDIVKNSGHRVEISRPEYYKPEIEKQVYEKEEKKVMDPDIYIR 368
Query: 421 QRERRDMKYHKRKL-QLQKDFHENDNLNVLKIAKLDE 528
E + +K L L+++ END++ +K LDE
Sbjct: 369 SYEESPNEVYKFSLTDLEEEIMENDSIEGVKCRMLDE 405
>At5g15920.1 68418.m01862 structural maintenance of chromosomes
(SMC) family protein (MSS2) similar to SMC-related
protein MSS2 [Arabidopsis thaliana] GI:9965743; contains
Pfam profiles PF02483: SMC family C-terminal domain,
PF02463: RecF/RecN/SMC N terminal domain
Length = 1053
Score = 30.3 bits (65), Expect = 1.4
Identities = 22/75 (29%), Positives = 45/75 (60%), Gaps = 3/75 (4%)
Frame = +1
Query: 376 KRAEIMNKCFQKYSEQRERRDM--KYHKRKLQLQKDFHENDNLNVLKIAKLDEQNNSAMI 549
+R EI+N Y E+++RR++ +Y +RK +L+ + ++++ +AKL +Q + A
Sbjct: 661 EREEIVNV---SYLEKKKRRELESRYQQRKTKLE-SLEQEEDMDA-SVAKLIDQASRANA 715
Query: 550 NRQTID-NMNRILEE 591
+R T N+ ++L E
Sbjct: 716 DRYTYAINLKKLLVE 730
>At1g08730.1 68414.m00969 myosin heavy chain (PCR43) identical to
myosin heavy chain PCR43 (PIR:T00727) [Arabidopsis
thaliana]; similar to ESTs gb|R30087 and gb|AA394762
Length = 1538
Score = 29.5 bits (63), Expect = 2.4
Identities = 35/134 (26%), Positives = 54/134 (40%), Gaps = 1/134 (0%)
Frame = +1
Query: 37 EILNRSITSKMRDSSIDETLAGFGKLRISALTKAVELSPLVKEATIGPNSPAKKELIYEE 216
EIL + + +DET A K R +A A E P++KE I K EL+ EE
Sbjct: 930 EILKLKSSFEEMRKKVDETNALLLKEREAAKKAAEEAPPVIKETQILVEDTKKIELMTEE 989
Query: 217 KQIPAKIEPLPEDLVEDKLRGDLGYNLIMKECEA-QLHQLSVEFFRKSFEQMICKRAEIM 393
+E + L +K R D + E+ + + +E K +Q+ +
Sbjct: 990 ------LESVKVTLENEKQRADDAVRKFEEAQESLEDKKKKLEETEKKGQQLQESLTRME 1043
Query: 394 NKCFQKYSEQRERR 435
KC SE + R
Sbjct: 1044 EKCSNLESENKVLR 1057
>At1g48360.1 68414.m05402 expressed protein ; expression supported
by MPSS
Length = 688
Score = 28.7 bits (61), Expect = 4.3
Identities = 20/73 (27%), Positives = 32/73 (43%), Gaps = 1/73 (1%)
Frame = +1
Query: 460 LQLQKDFHENDNLNVLKIA-KLDEQNNSAMINRQTIDNMNRILEEQNKATARFAAITDSH 636
LQ KD ++ +K A +LD Q + + + IL NK +R + D
Sbjct: 152 LQALKDLTVRGFMSSVKDANELDNQKMKEITELLNVTELRDILS-MNKVFSRTSRKRDLI 210
Query: 637 TKICICYNEITNV 675
+C CYN+ T +
Sbjct: 211 NSLCSCYNDGTRI 223
>At4g36270.1 68417.m05158 ATP-binding region, ATPase-like
domain-containing protein low similarity to microrchidia
[Mus musculus] GI:5410255; contains Pfam profile
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein
Length = 625
Score = 28.3 bits (60), Expect = 5.6
Identities = 15/54 (27%), Positives = 23/54 (42%)
Frame = +1
Query: 472 KDFHENDNLNVLKIAKLDEQNNSAMINRQTIDNMNRILEEQNKATARFAAITDS 633
K HE N+ + +E QT+ N+ + LEE AR A + D+
Sbjct: 550 KSAHEISEENIQLFMRCEEYVKKETELEQTVSNLAKELEETKSKCARLALLVDA 603
>At2g35330.1 68415.m04332 zinc finger (C3HC4-type RING finger)
protein-related contains weak hit to Pfam profile:
PF00097 zinc finger, C3HC4 type (RING finger); weak
similarity to RING finger protein 8 (Swiss-Prot:O76064)
[Homo sapiens]
Length = 738
Score = 28.3 bits (60), Expect = 5.6
Identities = 27/121 (22%), Positives = 48/121 (39%), Gaps = 5/121 (4%)
Frame = +1
Query: 361 EQMICKRAEIMNKCFQKYSEQRERRDMKYHKRKLQLQKDFHENDNLNVLKIAKLDEQNNS 540
EQ + E NK +K R + ++ + + K LQ+ E LN Q+N+
Sbjct: 587 EQRSKEAIEASNK--RKVESLRLKIEIDFQRHKDDLQRLEQELSRLNKASSTDSSLQSNN 644
Query: 541 AMINRQTIDN-----MNRILEEQNKATARFAAITDSHTKICICYNEITNVIASEPLSQQV 705
+ D M+++LEE N+ + + + IC + +V+ Q V
Sbjct: 645 TSHTKVKSDKSKGETMSKLLEELNRLDGSYEKEANYDRECLICMKDEVSVVFLPCAHQVV 704
Query: 706 C 708
C
Sbjct: 705 C 705
>At2g26870.1 68415.m03224 phosphoesterase family protein low
similarity to SP|Q9RGS8 Non-hemolytic phospholipase C
precursor (EC 3.1.4.3) (Phosphatidylcholine
cholinephosphohydrolase) {Burkholderia pseudomallei};
contains Pfam profile PF04185: Phosphoesterase family
Length = 514
Score = 28.3 bits (60), Expect = 5.6
Identities = 20/56 (35%), Positives = 27/56 (48%), Gaps = 2/56 (3%)
Frame = +1
Query: 85 DETLAGFGKLRISALTKAVELSPLVKEATI--GPNSPAKKELIYEEKQIPAKIEPL 246
D L F +L I T AV SP +++ T+ GPN YE IPA ++ L
Sbjct: 332 DPFLFQFNRLGIRVPTIAV--SPWIEKGTVVHGPNGSPFPSSEYEHSSIPATVKKL 385
>At1g64820.1 68414.m07349 MATE efflux family protein similar to
ripening regulated protein DDTFR18 [Lycopersicon
esculentum] GI:12231296; contains Pfam profile PF01554:
Uncharacterized membrane protein family; contains 12
transmembrane domains, PMID: 11152613
Length = 502
Score = 28.3 bits (60), Expect = 5.6
Identities = 11/38 (28%), Positives = 22/38 (57%)
Frame = -1
Query: 577 YSYCQLSAC*SLHCCSVHLIWLFLIHLNCHFHENPSVA 464
Y+Y + C + C S+ ++W F+ L FH++P ++
Sbjct: 110 YTYSSM-LCLLVFCFSISIVWFFMDKLLEIFHQDPLIS 146
>At5g47240.1 68418.m05825 MutT/nudix family protein similar to
SP|P53370 Nucleoside diphosphate-linked moiety X motif 6
{Homo sapiens}; contains Pfam profile PF00293: NUDIX
domain
Length = 369
Score = 27.9 bits (59), Expect = 7.4
Identities = 15/47 (31%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = +1
Query: 187 PAKKELIYEEKQIPA-KIEPLPEDLVEDKLRGDLGYNLIMKECEAQL 324
P ++I + +I A K PL E + + +RGD + +++ CEA+L
Sbjct: 278 PLSDKIIIDALEIKAAKWMPLAEFVEQPMIRGDKMFKRVIEICEARL 324
>At3g46200.1 68416.m05001 MutT/nudix family protein similar to head
organizer protein P17F11 GI:17976973 from [Xenopus
laevis]; contains a NUDIX hydrolase domain IPR000086
Length = 311
Score = 27.9 bits (59), Expect = 7.4
Identities = 16/53 (30%), Positives = 29/53 (54%)
Frame = +1
Query: 22 NDRLGEILNRSITSKMRDSSIDETLAGFGKLRISALTKAVELSPLVKEATIGP 180
N + GE+LN+ +T +M DS I E + G + S+L+ + + +E + P
Sbjct: 185 NVQTGEVLNKKVTQEMFDSIICEVVEETG-IPASSLSSPLFIGISRRELNVRP 236
>At1g68790.1 68414.m07863 expressed protein
Length = 1085
Score = 27.9 bits (59), Expect = 7.4
Identities = 26/106 (24%), Positives = 50/106 (47%), Gaps = 1/106 (0%)
Frame = +1
Query: 295 LIMKECEAQLHQLSVEFFRKSFEQMICKRAEIMNKCFQKYSEQRERRDMKYHKRKLQLQK 474
L++KE E +L Q E F K +E + KRA I + + E + R+++ ++ ++
Sbjct: 517 LLLKERE-ELKQ-DKERFEKEWEALDKKRANITREQNEVAEENEKLRNLQISEKHRLKRE 574
Query: 475 DFHENDNL-NVLKIAKLDEQNNSAMINRQTIDNMNRILEEQNKATA 609
+ DNL L K+ +++ A + + N +E Q + A
Sbjct: 575 EMTSRDNLKRELDGVKMQKESFEADMEDLEMQKRNLDMEFQRQEEA 620
>At5g18770.1 68418.m02230 F-box family protein contains F-box domain
Pfam:PF00646
Length = 481
Score = 27.5 bits (58), Expect = 9.8
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = -1
Query: 562 LSAC*SLHCCSVHLIWL 512
LSAC +L C +HL+WL
Sbjct: 151 LSACQALVCLKLHLVWL 167
>At3g05740.1 68416.m00644 DNA helicase (RECQI1) identical to DNA
Helicase [Arabidopsis thaliana] GI:10944747
Length = 606
Score = 27.5 bits (58), Expect = 9.8
Identities = 11/25 (44%), Positives = 19/25 (76%)
Frame = +1
Query: 328 QLSVEFFRKSFEQMICKRAEIMNKC 402
Q+ +E+F +SF++MICK + +N C
Sbjct: 578 QMLLEYFGESFDRMICKSS--LNPC 600
>At1g16060.2 68414.m01927 ovule development protein, putative
similar to ovule development protein AINTEGUMENTA
(GI:1209099)[Arabidopsis thaliana]
Length = 275
Score = 27.5 bits (58), Expect = 9.8
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +1
Query: 130 TKAVELSPLVKEATIGPNSPAKKELIYEEKQIPAKIE 240
TK ++ SPLV E IGP++P E+ + P I+
Sbjct: 203 TKLLKSSPLVAEEVIGPSTP--PEIAPPRRSFPEDIQ 237
>At1g16060.1 68414.m01926 ovule development protein, putative
similar to ovule development protein AINTEGUMENTA
(GI:1209099)[Arabidopsis thaliana]
Length = 345
Score = 27.5 bits (58), Expect = 9.8
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +1
Query: 130 TKAVELSPLVKEATIGPNSPAKKELIYEEKQIPAKIE 240
TK ++ SPLV E IGP++P E+ + P I+
Sbjct: 273 TKLLKSSPLVAEEVIGPSTP--PEIAPPRRSFPEDIQ 307
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.316 0.131 0.363
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,569,300
Number of Sequences: 28952
Number of extensions: 291388
Number of successful extensions: 862
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 840
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 862
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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