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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0522
         (697 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb...    31   0.16 
SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar...    30   0.28 
SPAC15E1.07c |moa1|mug159|meiotic cohesin complex associated pro...    29   0.64 
SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo...    29   0.64 
SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc...    29   0.64 
SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces pom...    28   1.1  
SPBP4H10.17c |||carboxyl methyl esterase|Schizosaccharomyces pom...    26   4.5  

>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1919

 Score = 31.1 bits (67), Expect = 0.16
 Identities = 14/36 (38%), Positives = 25/36 (69%)
 Frame = +1

Query: 568  ILSGDKKNIAHGALFLQDNLVKSDYGLSKENFNYFL 675
            ILSGD+ +++   +F QDN+   +Y  ++ +FN+FL
Sbjct: 1475 ILSGDELSLSEEDVFFQDNI---NYASNRYSFNFFL 1507


>SPBC23G7.08c |rga7||GTPase activating protein
           Rga7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 695

 Score = 30.3 bits (65), Expect = 0.28
 Identities = 27/96 (28%), Positives = 43/96 (44%), Gaps = 2/96 (2%)
 Frame = +1

Query: 34  EACLDLDKKKQGHKTSVRYLINISNNRNQEAIAAEIGFFHPRLDKEVVIKSNA-VFKVPE 210
           EA + +DK K   K +        +N+N      ++GFF P        KSNA + K+ +
Sbjct: 160 EAVMQMDKSKSRFKGAETEYNRALDNKNTGDSQKKVGFFKP--------KSNAQLTKLED 211

Query: 211 PNRYILESSVSLCHSSL-GADRVSKLLLDVSPTKFV 315
             R   E++ S  HS +  A  V K LL +    ++
Sbjct: 212 EARLKAENAESDMHSKIENAQNVQKQLLCIHRPNYI 247


>SPAC15E1.07c |moa1|mug159|meiotic cohesin complex associated
           protein Moa1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 172

 Score = 29.1 bits (62), Expect = 0.64
 Identities = 12/43 (27%), Positives = 23/43 (53%)
 Frame = +1

Query: 70  HKTSVRYLINISNNRNQEAIAAEIGFFHPRLDKEVVIKSNAVF 198
           H    R + NI N + +++I  +  ++H +   E +IK+ A F
Sbjct: 120 HVPDERLVSNIKNTQTKDSITRDSAYYHRKTMTESIIKTLAAF 162


>SPAC343.11c |msc1||multi-copy suppressor of Chk1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1588

 Score = 29.1 bits (62), Expect = 0.64
 Identities = 21/57 (36%), Positives = 27/57 (47%)
 Frame = -3

Query: 176 TTSLSSLGWKNPISAAIAS*FLLLEMLMRYLTEVLCPCFFLSRSRQASPLNIYGESL 6
           T S S  G++  IS  + S   LL  L    T   C C F S +  +SP + Y ESL
Sbjct: 406 TRSTSGKGFETKISINLDSDIKLLNTLSPLETFFWC-CSFPSTASTSSPFSYYPESL 461


>SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 758

 Score = 29.1 bits (62), Expect = 0.64
 Identities = 17/55 (30%), Positives = 24/55 (43%)
 Frame = +1

Query: 442 QALAKDFQYFEFTTGYLEEADRKLSIVGHLVPEKRVDITADIILSGDKKNIAHGA 606
           Q LA +    EF   Y    DR+  IV  + PE+   +    ++ GD     HGA
Sbjct: 94  QVLALENNIKEFGLTYYGMNDRRQGIVHVIGPEQGFTLPGTTLVCGDSHTSTHGA 148


>SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 781

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 13/35 (37%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
 Frame = -3

Query: 404 NLACWVFALLCTSTVPSKSIT-FTNGVWAKKTNLV 303
           N  CW+F  +     P++S++ FT+G+ AK+ NL+
Sbjct: 215 NFVCWLFDNM-EKDAPTESVSNFTDGINAKQLNLM 248


>SPBP4H10.17c |||carboxyl methyl esterase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 341

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 11/33 (33%), Positives = 20/33 (60%)
 Frame = +1

Query: 430 EVSVQALAKDFQYFEFTTGYLEEADRKLSIVGH 528
           ++S++ L+KDF +       + E D K+ +VGH
Sbjct: 120 DMSLETLSKDFTHAVSYVQRMFELDEKIILVGH 152


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.135    0.373 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,945,729
Number of Sequences: 5004
Number of extensions: 62456
Number of successful extensions: 190
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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