BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0522 (697 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 31 0.16 SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 30 0.28 SPAC15E1.07c |moa1|mug159|meiotic cohesin complex associated pro... 29 0.64 SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo... 29 0.64 SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc... 29 0.64 SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces pom... 28 1.1 SPBP4H10.17c |||carboxyl methyl esterase|Schizosaccharomyces pom... 26 4.5 >SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 31.1 bits (67), Expect = 0.16 Identities = 14/36 (38%), Positives = 25/36 (69%) Frame = +1 Query: 568 ILSGDKKNIAHGALFLQDNLVKSDYGLSKENFNYFL 675 ILSGD+ +++ +F QDN+ +Y ++ +FN+FL Sbjct: 1475 ILSGDELSLSEEDVFFQDNI---NYASNRYSFNFFL 1507 >SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 30.3 bits (65), Expect = 0.28 Identities = 27/96 (28%), Positives = 43/96 (44%), Gaps = 2/96 (2%) Frame = +1 Query: 34 EACLDLDKKKQGHKTSVRYLINISNNRNQEAIAAEIGFFHPRLDKEVVIKSNA-VFKVPE 210 EA + +DK K K + +N+N ++GFF P KSNA + K+ + Sbjct: 160 EAVMQMDKSKSRFKGAETEYNRALDNKNTGDSQKKVGFFKP--------KSNAQLTKLED 211 Query: 211 PNRYILESSVSLCHSSL-GADRVSKLLLDVSPTKFV 315 R E++ S HS + A V K LL + ++ Sbjct: 212 EARLKAENAESDMHSKIENAQNVQKQLLCIHRPNYI 247 >SPAC15E1.07c |moa1|mug159|meiotic cohesin complex associated protein Moa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 172 Score = 29.1 bits (62), Expect = 0.64 Identities = 12/43 (27%), Positives = 23/43 (53%) Frame = +1 Query: 70 HKTSVRYLINISNNRNQEAIAAEIGFFHPRLDKEVVIKSNAVF 198 H R + NI N + +++I + ++H + E +IK+ A F Sbjct: 120 HVPDERLVSNIKNTQTKDSITRDSAYYHRKTMTESIIKTLAAF 162 >SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1588 Score = 29.1 bits (62), Expect = 0.64 Identities = 21/57 (36%), Positives = 27/57 (47%) Frame = -3 Query: 176 TTSLSSLGWKNPISAAIAS*FLLLEMLMRYLTEVLCPCFFLSRSRQASPLNIYGESL 6 T S S G++ IS + S LL L T C C F S + +SP + Y ESL Sbjct: 406 TRSTSGKGFETKISINLDSDIKLLNTLSPLETFFWC-CSFPSTASTSSPFSYYPESL 461 >SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 758 Score = 29.1 bits (62), Expect = 0.64 Identities = 17/55 (30%), Positives = 24/55 (43%) Frame = +1 Query: 442 QALAKDFQYFEFTTGYLEEADRKLSIVGHLVPEKRVDITADIILSGDKKNIAHGA 606 Q LA + EF Y DR+ IV + PE+ + ++ GD HGA Sbjct: 94 QVLALENNIKEFGLTYYGMNDRRQGIVHVIGPEQGFTLPGTTLVCGDSHTSTHGA 148 >SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 781 Score = 28.3 bits (60), Expect = 1.1 Identities = 13/35 (37%), Positives = 23/35 (65%), Gaps = 1/35 (2%) Frame = -3 Query: 404 NLACWVFALLCTSTVPSKSIT-FTNGVWAKKTNLV 303 N CW+F + P++S++ FT+G+ AK+ NL+ Sbjct: 215 NFVCWLFDNM-EKDAPTESVSNFTDGINAKQLNLM 248 >SPBP4H10.17c |||carboxyl methyl esterase|Schizosaccharomyces pombe|chr 2|||Manual Length = 341 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/33 (33%), Positives = 20/33 (60%) Frame = +1 Query: 430 EVSVQALAKDFQYFEFTTGYLEEADRKLSIVGH 528 ++S++ L+KDF + + E D K+ +VGH Sbjct: 120 DMSLETLSKDFTHAVSYVQRMFELDEKIILVGH 152 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.135 0.373 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,945,729 Number of Sequences: 5004 Number of extensions: 62456 Number of successful extensions: 190 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 190 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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