BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0521 (656 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC14F5.05c |sam1||S-adenosylmethionine synthetase |Schizosacch... 191 7e-50 SPAC1B3.09c |||Noc2p-Noc3p complex subunit Noc2 family |Schizosa... 28 1.0 SPBC660.14 |mik1||mitotic inhibitor kinase Mik1|Schizosaccharomy... 25 7.3 SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|ch... 25 7.3 >SPBC14F5.05c |sam1||S-adenosylmethionine synthetase |Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 191 bits (466), Expect = 7e-50 Identities = 90/142 (63%), Positives = 105/142 (73%) Frame = +3 Query: 3 IGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFTKVDRSAAYAARWVAKSLVKAGLC 182 +GGPQ DAGLTGRKIIVDTY KD++KVDRSAAYAARW+AKSLV AGL Sbjct: 239 VGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYSKVDRSAAYAARWIAKSLVAAGLA 298 Query: 183 RRCMVQVAYAIGVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRAPIY 362 RRC+VQ++YAIGVAEPLSI V YGTS KT +L+ I++KNFDLRPG +VK L L+ P Y Sbjct: 299 RRCLVQLSYAIGVAEPLSIFVNTYGTSSKTSAELVEIIRKNFDLRPGVLVKSLKLQTPFY 358 Query: 363 QRTSTYGHFGRAGFPWESPKPL 428 T++YGHF PWE PK L Sbjct: 359 LSTASYGHFTDQSKPWEQPKEL 380 >SPAC1B3.09c |||Noc2p-Noc3p complex subunit Noc2 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 528 Score = 28.3 bits (60), Expect = 1.0 Identities = 14/32 (43%), Positives = 20/32 (62%) Frame = -2 Query: 529 SALDTMTHIHKCLLYTDGIFTLF*CSGFASHS 434 SALDT+ HI K + +G+ T C+ F +HS Sbjct: 178 SALDTILHIKKFSKFPNGLVTQL-CNIFVNHS 208 >SPBC660.14 |mik1||mitotic inhibitor kinase Mik1|Schizosaccharomyces pombe|chr 2|||Manual Length = 581 Score = 25.4 bits (53), Expect = 7.3 Identities = 12/44 (27%), Positives = 20/44 (45%), Gaps = 2/44 (4%) Frame = +2 Query: 323 QNRQRVEPQSTNLSENKYLWTLWKGRI--PLGESQAADCRMTCK 448 + ++ V ST L + W+LW+G + P E C+ K Sbjct: 123 KRKRAVTSLSTGLLSKREKWSLWEGNLTNPRSEQPHTPCKKGTK 166 >SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 752 Score = 25.4 bits (53), Expect = 7.3 Identities = 14/42 (33%), Positives = 22/42 (52%) Frame = +2 Query: 272 SATTALHRTKEFRFTTGQNRQRVEPQSTNLSENKYLWTLWKG 397 SA A + F G++ ++P S++L KY+ TL KG Sbjct: 255 SAIQAFESILQNTFFNGKSNPTIDPNSSSLLSEKYI-TLDKG 295 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,662,270 Number of Sequences: 5004 Number of extensions: 55473 Number of successful extensions: 118 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 117 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 118 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 297805304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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