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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0521
         (656 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati...   144   5e-35
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2...   141   3e-34
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati...   141   3e-34
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1...   136   2e-32
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1...   136   2e-32
At5g32482.1 68418.m03835 zinc knuckle (CCHC-type) family protein...    31   0.51 
At4g14780.1 68417.m02273 protein kinase, putative similar to pro...    29   3.6  
At2g36380.1 68415.m04464 ABC transporter family protein related ...    27   8.3  

>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
           similar to S-adenosylmethionine synthetase 2 (Methionine
           adenosyltransferase 2, AdoMet synthetase 2)
           [Catharanthus roseus] SWISS-PROT:Q96552
          Length = 393

 Score =  144 bits (349), Expect = 5e-35
 Identities = 77/147 (52%), Positives = 94/147 (63%), Gaps = 5/147 (3%)
 Frame = +3

Query: 3   IGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFTKVDRSAAYAARWVAKSLVKAGLC 182
           IGGP  DAGLTGRKII+DTY             KD TKVDRS AY  R  AKS+V +GL 
Sbjct: 240 IGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLA 299

Query: 183 RRCMVQVAYAIGVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRA--- 353
           RR +VQV+YAIGV EPLS+ V  YGT     +++L IV+++FD RPG I   L+L+    
Sbjct: 300 RRVIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILEIVKESFDFRPGMISINLDLKRGGN 359

Query: 354 PIYQRTSTYGHFGR--AGFPWESPKPL 428
             + +T+ YGHFGR  A F WE  KPL
Sbjct: 360 GRFLKTAAYGHFGRDDADFTWEVVKPL 386


>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
           identical to S-adenosylmethionine synthetase 2
           (Methionine adenosyltransferase 2, AdoMet synthetase 2)
           [Arabidopsis thaliana] SWISS-PROT:P17562
          Length = 393

 Score =  141 bits (342), Expect = 3e-34
 Identities = 75/147 (51%), Positives = 92/147 (62%), Gaps = 5/147 (3%)
 Frame = +3

Query: 3   IGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFTKVDRSAAYAARWVAKSLVKAGLC 182
           IGGP  DAGLTGRKII+DTY             KD TKVDRS AY  R  AKS+V  G+ 
Sbjct: 240 IGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVANGMA 299

Query: 183 RRCMVQVAYAIGVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRA--- 353
           RR +VQV+YAIGV EPLS+ V  YGT     +++L IV++ FD RPG +   L+L+    
Sbjct: 300 RRALVQVSYAIGVPEPLSVFVDTYGTGLIPDKEILKIVKETFDFRPGMMTINLDLKRGGN 359

Query: 354 PIYQRTSTYGHFGR--AGFPWESPKPL 428
             +Q+T+ YGHFGR    F WE  KPL
Sbjct: 360 GRFQKTAAYGHFGRDDPDFTWEVVKPL 386


>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
           similar to S-adenosylmethionine synthetase 3 (Methionine
           adenosyltransferase 3, AdoMet synthetase 3)
           [Lycopersicon esculentum] SWISS-PROT:P43282
          Length = 390

 Score =  141 bits (342), Expect = 3e-34
 Identities = 75/147 (51%), Positives = 92/147 (62%), Gaps = 5/147 (3%)
 Frame = +3

Query: 3   IGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFTKVDRSAAYAARWVAKSLVKAGLC 182
           IGGP  DAGLTGRKII+DTY             KD TKVDRS AY  R  AKS+V AGL 
Sbjct: 240 IGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVAAGLA 299

Query: 183 RRCMVQVAYAIGVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRAP-- 356
           RRC+VQV+YAIGV EPLS+ V  Y T     + +L ++++ FD RPG +   L+L+    
Sbjct: 300 RRCIVQVSYAIGVPEPLSVFVDTYKTGTIPDKDILVLIKEAFDFRPGMMAINLDLKRGGN 359

Query: 357 -IYQRTSTYGHFGR--AGFPWESPKPL 428
             +Q+T+ YGHFGR    F WE  KPL
Sbjct: 360 FRFQKTAAYGHFGRDDPDFTWEVVKPL 386


>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
           identical to S-adenosylmethionine synthetase 1
           (Methionine adenosyltransferase 1, AdoMet synthetase 1)
           [Arabidopsis thaliana] SWISS-PROT:P23686
          Length = 393

 Score =  136 bits (328), Expect = 2e-32
 Identities = 73/147 (49%), Positives = 91/147 (61%), Gaps = 5/147 (3%)
 Frame = +3

Query: 3   IGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFTKVDRSAAYAARWVAKSLVKAGLC 182
           IGGP  DAGLTGRKII+DTY             KD TKVDRS AY  R  AKS+V  G+ 
Sbjct: 240 IGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVANGMA 299

Query: 183 RRCMVQVAYAIGVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRA--- 353
           RR +VQV+YAIGV EPLS+ V  Y T     +++L IV+++FD RPG +   L+L+    
Sbjct: 300 RRALVQVSYAIGVPEPLSVFVDTYETGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGN 359

Query: 354 PIYQRTSTYGHFGR--AGFPWESPKPL 428
             + +T+ YGHFGR    F WE  KPL
Sbjct: 360 GRFLKTAAYGHFGRDDPDFTWEVVKPL 386


>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
           identical to S-adenosylmethionine synthetase 1
           (Methionine adenosyltransferase 1, AdoMet synthetase 1)
           [Arabidopsis thaliana] SWISS-PROT:P23686
          Length = 393

 Score =  136 bits (328), Expect = 2e-32
 Identities = 73/147 (49%), Positives = 91/147 (61%), Gaps = 5/147 (3%)
 Frame = +3

Query: 3   IGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFTKVDRSAAYAARWVAKSLVKAGLC 182
           IGGP  DAGLTGRKII+DTY             KD TKVDRS AY  R  AKS+V  G+ 
Sbjct: 240 IGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVANGMA 299

Query: 183 RRCMVQVAYAIGVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRA--- 353
           RR +VQV+YAIGV EPLS+ V  Y T     +++L IV+++FD RPG +   L+L+    
Sbjct: 300 RRALVQVSYAIGVPEPLSVFVDTYETGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGN 359

Query: 354 PIYQRTSTYGHFGR--AGFPWESPKPL 428
             + +T+ YGHFGR    F WE  KPL
Sbjct: 360 GRFLKTAAYGHFGRDDPDFTWEVVKPL 386


>At5g32482.1 68418.m03835 zinc knuckle (CCHC-type) family protein
           contains Pfam domain, PF00098: Zinc knuckle
          Length = 341

 Score = 31.5 bits (68), Expect = 0.51
 Identities = 23/62 (37%), Positives = 27/62 (43%)
 Frame = +2

Query: 35  GTQNHRGHVWRVGGARRGRFLWQGLHQGRPLRSIRRSMGRQVARQGGLVPALHGAGCLCY 214
           G  + RGH    GG  RG F  QG H G   R  R + GR   R  G+      A  +CY
Sbjct: 223 GNPHGRGHGRGRGGRGRGNFYGQGNHYGG--RG-RGNYGRSRGRGRGINKPRGKAKSVCY 279

Query: 215 WC 220
            C
Sbjct: 280 RC 281


>At4g14780.1 68417.m02273 protein kinase, putative similar to
           protein kinase ATMRK1 [Arabidopsis thaliana]
           gi|2351097|dbj|BAA22079
          Length = 364

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 12/41 (29%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
 Frame = +2

Query: 98  WQGLHQGRP-LRSIRRSM-GRQVARQGGLVPALHGAGCLCY 214
           W G  Q RP ++ + + + G   ++ GG++P     GC C+
Sbjct: 318 WDGNPQKRPEMKEVVKMLEGVDTSKGGGMIPEDQSRGCFCF 358


>At2g36380.1 68415.m04464 ABC transporter family protein related to
           multi drug resistance proteins and P-glycoproteins
          Length = 1453

 Score = 27.5 bits (58), Expect = 8.3
 Identities = 15/45 (33%), Positives = 21/45 (46%)
 Frame = -1

Query: 230 WLSYTNSISNLHHAAPAQARLDERLGDPSSGVCCGAVDLGEVLAR 96
           W  YT+ +     A      LDER G P++     A  +GEVL +
Sbjct: 718 WCYYTSPMMYGQTALVINEFLDERWGSPNNDTRINAKTVGEVLLK 762


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,055,049
Number of Sequences: 28952
Number of extensions: 299042
Number of successful extensions: 674
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 649
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 669
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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