BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0518 (713 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 69 7e-13 SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces po... 59 7e-10 SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual 51 2e-07 SPBC31F10.07 |||cortical component Lsb5 |Schizosaccharomyces pom... 48 1e-06 SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual 46 4e-06 SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 27 2.0 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 27 2.0 SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 2.0 SPAC630.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 2.7 SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom... 26 6.1 SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 26 6.1 SPAC4D7.08c |ade4|min13, aza1|amidophosphoribosyltransferase |Sc... 26 6.1 SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 25 8.1 SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe... 25 8.1 SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak... 25 8.1 >SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated membrane proteins, ESCRT 0 complex|Schizosaccharomyces pombe|chr 1|||Manual Length = 610 Score = 68.9 bits (161), Expect = 7e-13 Identities = 37/89 (41%), Positives = 53/89 (59%), Gaps = 2/89 (2%) Frame = +3 Query: 258 FGTSSPFDQDVERATSENNT--SEEWGLIMEICDRAGASSTSAKECLRAVMRRLAHPDPH 431 + ++S F D+E+ATSE SEE L +EI D+ + S K +R + R+ H +P+ Sbjct: 5 WNSNSQFASDIEKATSETLPAGSEEISLYLEISDQIRSKSVDPKFAMRILKSRIDHSNPN 64 Query: 432 VQVHAATLLDACVANCGRFFHLEVASRDF 518 VQ+ A L D CV N G F LE+ASR+F Sbjct: 65 VQIMALKLTDTCVKNGGSGFLLEIASREF 93 >SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces pombe|chr 2|||Manual Length = 373 Score = 58.8 bits (136), Expect = 7e-10 Identities = 36/129 (27%), Positives = 63/129 (48%), Gaps = 3/129 (2%) Frame = +3 Query: 261 GTSSPFDQDVERATSENNTSEEWGLIMEICDRAGASSTS-AKECLRAVMRRLAHPDPHVQ 437 G + + + +AT E NT E+W +IM+ CD+ ++S + ++ + +RL + ++Q Sbjct: 4 GKPNSIETLILQATDEKNTKEKWDVIMDACDQLSSTSGDVGRNSIKFLNKRLDTANANIQ 63 Query: 438 VHAATLLDACVANCGRFFHLEVASRDFETEFRRLL--SRAQPPVXXXXXXXXXKWAEGEF 611 + A TL DA V NC E++SR F ++ S V +WAE Sbjct: 64 LLALTLTDAIVKNCKTSIVREISSRTFTDSLLKIASDSTTHNRVRSRIAVLVNEWAE-IM 122 Query: 612 RDDPQLDLI 638 + DP + L+ Sbjct: 123 KKDPNMSLM 131 >SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual Length = 510 Score = 50.8 bits (116), Expect = 2e-07 Identities = 27/90 (30%), Positives = 44/90 (48%), Gaps = 1/90 (1%) Frame = +3 Query: 264 TSSPFDQDVERATSENNTSEEWGLIMEICDRAGASS-TSAKECLRAVMRRLAHPDPHVQV 440 +S + +++AT + N L +EI D + +E +++R+ +P V Sbjct: 3 SSQTLSKYIDKATDQFNLEPNLALNIEIADLINEKKGNTPREAALLILKRVNSANPTVSY 62 Query: 441 HAATLLDACVANCGRFFHLEVASRDFETEF 530 A LLD CV NCG FH ++AS +F F Sbjct: 63 LALHLLDICVKNCGYPFHFQIASEEFLNGF 92 >SPBC31F10.07 |||cortical component Lsb5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 304 Score = 48.0 bits (109), Expect = 1e-06 Identities = 32/143 (22%), Positives = 62/143 (43%), Gaps = 5/143 (3%) Frame = +3 Query: 249 MGIFGTSSPFDQD---VERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAVMRRLAH 419 MGIF + P ++R TS + E+ I+++ + + T +E R + ++L + Sbjct: 1 MGIFSETVPITAVTTYIDRLTSRDTDDEDLSGIVQLSEAVNLTVTGPREASRTLRKKLKY 60 Query: 420 PDPHVQVHAATLLDACVANCGRFFHLEVASRDFETEFRRLLSRAQ--PPVXXXXXXXXXK 593 PH QV A +L A + N G F + E + + ++ PV Sbjct: 61 STPHEQVRALVILQALIENAGSHFLQNFSDEKLEDRMLQCATNSEYSKPVRKRAIHMIKL 120 Query: 594 WAEGEFRDDPQLDLIPALHARLP 662 W ++ + ++ + +L +RLP Sbjct: 121 W-HNDYSNVRGMESMSSLVSRLP 142 >SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual Length = 533 Score = 46.4 bits (105), Expect = 4e-06 Identities = 26/91 (28%), Positives = 44/91 (48%), Gaps = 1/91 (1%) Frame = +3 Query: 288 VERATSENNTSEEWGLIMEICDRAGASSTSA-KECLRAVMRRLAHPDPHVQVHAATLLDA 464 ++ AT + + ++I D + + +E A++R++ +P V A LLD Sbjct: 12 IQNATEPYAFEPDLAVNLDIADLINQTGGNLPREAAFAIVRKVNDRNPTVAYLALNLLDI 71 Query: 465 CVANCGRFFHLEVASRDFETEFRRLLSRAQP 557 CV NCG F L++AS++F E R P Sbjct: 72 CVKNCGYAFRLQIASKEFLNELVRRFPERPP 102 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 27.5 bits (58), Expect = 2.0 Identities = 15/54 (27%), Positives = 26/54 (48%) Frame = +3 Query: 402 MRRLAHPDPHVQVHAATLLDACVANCGRFFHLEVASRDFETEFRRLLSRAQPPV 563 ++RL+ DP V + + VA G HLE+ +D + + + + PPV Sbjct: 508 LKRLSKSDPCVLCTTSESGEHIVAGAGEL-HLEICLKDLQEDHAGIPLKISPPV 560 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 27.5 bits (58), Expect = 2.0 Identities = 15/54 (27%), Positives = 26/54 (48%) Frame = +3 Query: 402 MRRLAHPDPHVQVHAATLLDACVANCGRFFHLEVASRDFETEFRRLLSRAQPPV 563 ++RL+ DP V + + VA G HLE+ +D + + + + PPV Sbjct: 508 LKRLSKSDPCVLCTTSESGEHIVAGAGEL-HLEICLKDLQEDHAGIPLKISPPV 560 >SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosaccharomyces pombe|chr 2|||Manual Length = 948 Score = 27.5 bits (58), Expect = 2.0 Identities = 17/61 (27%), Positives = 28/61 (45%) Frame = +1 Query: 418 IPTHTYRFTPPHCSTPALQIVVVSSTWKSPRETSRPSSGACCLARSLLSPLVSAPCSASG 597 +PT T + STP +S+ S ++++ S +A S L P S+P + S Sbjct: 263 VPTQTIDSSSFTSSTPVSLTSSSTSSSGSSQDSTTIDSTPSTIATSTLQPTTSSPITTSA 322 Query: 598 P 600 P Sbjct: 323 P 323 >SPAC630.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 27.1 bits (57), Expect = 2.7 Identities = 19/52 (36%), Positives = 26/52 (50%), Gaps = 8/52 (15%) Frame = +2 Query: 383 GVSPCRHAALGASRPTRTGSRRHTARRLR----C----KLWSFLPPGSRLAR 514 GVS C++AA GAS P + A++L C KLW+ P S L + Sbjct: 234 GVSHCKYAAAGASYPLPIPASGLLAQKLAIGLLCKDIIKLWNLCPESSILTK 285 >SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 772 Score = 25.8 bits (54), Expect = 6.1 Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 3/46 (6%) Frame = +1 Query: 448 PHCSTPALQIVVVSSTWKSPRET---SRPSSGACCLARSLLSPLVS 576 P CSTP++ VS+T + ET S +S + RS LS L+S Sbjct: 281 PRCSTPSVSSTFVSATSEPEVETYSVSTKNSSSNKNLRSSLSKLLS 326 >SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 25.8 bits (54), Expect = 6.1 Identities = 13/37 (35%), Positives = 23/37 (62%) Frame = -2 Query: 307 SLVARSTS*SKGEEVPNIPILLTKKHLLDYMLLIKIL 197 SL+A + S ++ ++ + LT++ L DY +IKIL Sbjct: 318 SLIAFNVSITEDADIIVVSTFLTEEGLTDYQRVIKIL 354 >SPAC4D7.08c |ade4|min13, aza1|amidophosphoribosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 25.8 bits (54), Expect = 6.1 Identities = 15/33 (45%), Positives = 19/33 (57%) Frame = -2 Query: 397 ARRHSFALVEDAPARSHISMISPHSSLVLFSLV 299 AR+HSFA E+ A IS+ + HS V F V Sbjct: 502 ARKHSFAEDEEREAPEDISLHNTHSD-VTFDFV 533 >SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|Schizosaccharomyces pombe|chr 1|||Manual Length = 2397 Score = 25.4 bits (53), Expect = 8.1 Identities = 19/59 (32%), Positives = 25/59 (42%) Frame = +3 Query: 342 EICDRAGASSTSAKECLRAVMRRLAHPDPHVQVHAATLLDACVANCGRFFHLEVASRDF 518 E C +A SS S + LRA+ P + LD V NC +F H + DF Sbjct: 1573 EACRQALTSSKSVRTKLRAISTIQRFPVSEW----VSKLDTHVRNCIKFSHKQNLEEDF 1627 >SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe|chr 1|||Manual Length = 371 Score = 25.4 bits (53), Expect = 8.1 Identities = 10/30 (33%), Positives = 19/30 (63%) Frame = +3 Query: 258 FGTSSPFDQDVERATSENNTSEEWGLIMEI 347 FG++ P D++ + T+ +NTS + L +I Sbjct: 331 FGSTEPIDEEEKSKTASDNTSLQTSLFGKI 360 >SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 708 Score = 25.4 bits (53), Expect = 8.1 Identities = 22/87 (25%), Positives = 38/87 (43%), Gaps = 1/87 (1%) Frame = -1 Query: 353 ITYLHDQSPLLAGVIFTRCAFNILIKRRGSAKYPHF-VNKKTST*LYVIDKNIK*FLILK 177 +TYLH + +L R K P F +++K Y+ LI Sbjct: 546 VTYLHRILNAIKETYSGYLLLTVLCMRLNIIKTPSFTLDEKIQESYYMAHYRP---LINL 602 Query: 176 SE*NRILQTKISCFMKNLRNKNTDYII 96 + +L+++ C +KNL+N +TD +I Sbjct: 603 RQPKPLLRSEADCIIKNLQNFSTDDLI 629 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,439,753 Number of Sequences: 5004 Number of extensions: 48049 Number of successful extensions: 144 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 140 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 143 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 333194204 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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