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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0518
         (713 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb...    69   7e-13
SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces po...    59   7e-10
SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual        51   2e-07
SPBC31F10.07 |||cortical component Lsb5 |Schizosaccharomyces pom...    48   1e-06
SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual       46   4e-06
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...    27   2.0  
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...    27   2.0  
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch...    27   2.0  
SPAC630.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    27   2.7  
SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom...    26   6.1  
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe...    26   6.1  
SPAC4D7.08c |ade4|min13, aza1|amidophosphoribosyltransferase |Sc...    26   6.1  
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S...    25   8.1  
SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe...    25   8.1  
SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak...    25   8.1  

>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
           membrane proteins, ESCRT 0 complex|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 610

 Score = 68.9 bits (161), Expect = 7e-13
 Identities = 37/89 (41%), Positives = 53/89 (59%), Gaps = 2/89 (2%)
 Frame = +3

Query: 258 FGTSSPFDQDVERATSENNT--SEEWGLIMEICDRAGASSTSAKECLRAVMRRLAHPDPH 431
           + ++S F  D+E+ATSE     SEE  L +EI D+  + S   K  +R +  R+ H +P+
Sbjct: 5   WNSNSQFASDIEKATSETLPAGSEEISLYLEISDQIRSKSVDPKFAMRILKSRIDHSNPN 64

Query: 432 VQVHAATLLDACVANCGRFFHLEVASRDF 518
           VQ+ A  L D CV N G  F LE+ASR+F
Sbjct: 65  VQIMALKLTDTCVKNGGSGFLLEIASREF 93


>SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 373

 Score = 58.8 bits (136), Expect = 7e-10
 Identities = 36/129 (27%), Positives = 63/129 (48%), Gaps = 3/129 (2%)
 Frame = +3

Query: 261 GTSSPFDQDVERATSENNTSEEWGLIMEICDRAGASSTS-AKECLRAVMRRLAHPDPHVQ 437
           G  +  +  + +AT E NT E+W +IM+ CD+  ++S    +  ++ + +RL   + ++Q
Sbjct: 4   GKPNSIETLILQATDEKNTKEKWDVIMDACDQLSSTSGDVGRNSIKFLNKRLDTANANIQ 63

Query: 438 VHAATLLDACVANCGRFFHLEVASRDFETEFRRLL--SRAQPPVXXXXXXXXXKWAEGEF 611
           + A TL DA V NC      E++SR F     ++   S     V         +WAE   
Sbjct: 64  LLALTLTDAIVKNCKTSIVREISSRTFTDSLLKIASDSTTHNRVRSRIAVLVNEWAE-IM 122

Query: 612 RDDPQLDLI 638
           + DP + L+
Sbjct: 123 KKDPNMSLM 131


>SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 510

 Score = 50.8 bits (116), Expect = 2e-07
 Identities = 27/90 (30%), Positives = 44/90 (48%), Gaps = 1/90 (1%)
 Frame = +3

Query: 264 TSSPFDQDVERATSENNTSEEWGLIMEICDRAGASS-TSAKECLRAVMRRLAHPDPHVQV 440
           +S    + +++AT + N      L +EI D        + +E    +++R+   +P V  
Sbjct: 3   SSQTLSKYIDKATDQFNLEPNLALNIEIADLINEKKGNTPREAALLILKRVNSANPTVSY 62

Query: 441 HAATLLDACVANCGRFFHLEVASRDFETEF 530
            A  LLD CV NCG  FH ++AS +F   F
Sbjct: 63  LALHLLDICVKNCGYPFHFQIASEEFLNGF 92


>SPBC31F10.07 |||cortical component Lsb5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 304

 Score = 48.0 bits (109), Expect = 1e-06
 Identities = 32/143 (22%), Positives = 62/143 (43%), Gaps = 5/143 (3%)
 Frame = +3

Query: 249 MGIFGTSSPFDQD---VERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAVMRRLAH 419
           MGIF  + P       ++R TS +   E+   I+++ +    + T  +E  R + ++L +
Sbjct: 1   MGIFSETVPITAVTTYIDRLTSRDTDDEDLSGIVQLSEAVNLTVTGPREASRTLRKKLKY 60

Query: 420 PDPHVQVHAATLLDACVANCGRFFHLEVASRDFETEFRRLLSRAQ--PPVXXXXXXXXXK 593
             PH QV A  +L A + N G  F    +    E    +  + ++   PV          
Sbjct: 61  STPHEQVRALVILQALIENAGSHFLQNFSDEKLEDRMLQCATNSEYSKPVRKRAIHMIKL 120

Query: 594 WAEGEFRDDPQLDLIPALHARLP 662
           W   ++ +   ++ + +L +RLP
Sbjct: 121 W-HNDYSNVRGMESMSSLVSRLP 142


>SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 533

 Score = 46.4 bits (105), Expect = 4e-06
 Identities = 26/91 (28%), Positives = 44/91 (48%), Gaps = 1/91 (1%)
 Frame = +3

Query: 288 VERATSENNTSEEWGLIMEICDRAGASSTSA-KECLRAVMRRLAHPDPHVQVHAATLLDA 464
           ++ AT       +  + ++I D    +  +  +E   A++R++   +P V   A  LLD 
Sbjct: 12  IQNATEPYAFEPDLAVNLDIADLINQTGGNLPREAAFAIVRKVNDRNPTVAYLALNLLDI 71

Query: 465 CVANCGRFFHLEVASRDFETEFRRLLSRAQP 557
           CV NCG  F L++AS++F  E  R      P
Sbjct: 72  CVKNCGYAFRLQIASKEFLNELVRRFPERPP 102


>SPCP31B10.07 |eft202||translation elongation factor 2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 15/54 (27%), Positives = 26/54 (48%)
 Frame = +3

Query: 402 MRRLAHPDPHVQVHAATLLDACVANCGRFFHLEVASRDFETEFRRLLSRAQPPV 563
           ++RL+  DP V    +   +  VA  G   HLE+  +D + +   +  +  PPV
Sbjct: 508 LKRLSKSDPCVLCTTSESGEHIVAGAGEL-HLEICLKDLQEDHAGIPLKISPPV 560


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
           elongation factor 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 15/54 (27%), Positives = 26/54 (48%)
 Frame = +3

Query: 402 MRRLAHPDPHVQVHAATLLDACVANCGRFFHLEVASRDFETEFRRLLSRAQPPV 563
           ++RL+  DP V    +   +  VA  G   HLE+  +D + +   +  +  PPV
Sbjct: 508 LKRLSKSDPCVLCTTSESGEHIVAGAGEL-HLEICLKDLQEDHAGIPLKISPPV 560


>SPBC21D10.06c |map4||cell agglutination protein
           Map4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 948

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 17/61 (27%), Positives = 28/61 (45%)
 Frame = +1

Query: 418 IPTHTYRFTPPHCSTPALQIVVVSSTWKSPRETSRPSSGACCLARSLLSPLVSAPCSASG 597
           +PT T   +    STP       +S+  S ++++   S    +A S L P  S+P + S 
Sbjct: 263 VPTQTIDSSSFTSSTPVSLTSSSTSSSGSSQDSTTIDSTPSTIATSTLQPTTSSPITTSA 322

Query: 598 P 600
           P
Sbjct: 323 P 323


>SPAC630.07c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 430

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 19/52 (36%), Positives = 26/52 (50%), Gaps = 8/52 (15%)
 Frame = +2

Query: 383 GVSPCRHAALGASRPTRTGSRRHTARRLR----C----KLWSFLPPGSRLAR 514
           GVS C++AA GAS P    +    A++L     C    KLW+  P  S L +
Sbjct: 234 GVSHCKYAAAGASYPLPIPASGLLAQKLAIGLLCKDIIKLWNLCPESSILTK 285


>SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 772

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 3/46 (6%)
 Frame = +1

Query: 448 PHCSTPALQIVVVSSTWKSPRET---SRPSSGACCLARSLLSPLVS 576
           P CSTP++    VS+T +   ET   S  +S +    RS LS L+S
Sbjct: 281 PRCSTPSVSSTFVSATSEPEVETYSVSTKNSSSNKNLRSSLSKLLS 326


>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 969

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 13/37 (35%), Positives = 23/37 (62%)
 Frame = -2

Query: 307 SLVARSTS*SKGEEVPNIPILLTKKHLLDYMLLIKIL 197
           SL+A + S ++  ++  +   LT++ L DY  +IKIL
Sbjct: 318 SLIAFNVSITEDADIIVVSTFLTEEGLTDYQRVIKIL 354


>SPAC4D7.08c |ade4|min13, aza1|amidophosphoribosyltransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 533

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 15/33 (45%), Positives = 19/33 (57%)
 Frame = -2

Query: 397 ARRHSFALVEDAPARSHISMISPHSSLVLFSLV 299
           AR+HSFA  E+  A   IS+ + HS  V F  V
Sbjct: 502 ARKHSFAEDEEREAPEDISLHNTHSD-VTFDFV 533


>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
            Mok11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2397

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 19/59 (32%), Positives = 25/59 (42%)
 Frame = +3

Query: 342  EICDRAGASSTSAKECLRAVMRRLAHPDPHVQVHAATLLDACVANCGRFFHLEVASRDF 518
            E C +A  SS S +  LRA+      P         + LD  V NC +F H +    DF
Sbjct: 1573 EACRQALTSSKSVRTKLRAISTIQRFPVSEW----VSKLDTHVRNCIKFSHKQNLEEDF 1627


>SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 371

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 10/30 (33%), Positives = 19/30 (63%)
 Frame = +3

Query: 258 FGTSSPFDQDVERATSENNTSEEWGLIMEI 347
           FG++ P D++ +  T+ +NTS +  L  +I
Sbjct: 331 FGSTEPIDEEEKSKTASDNTSLQTSLFGKI 360


>SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak10
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 708

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 22/87 (25%), Positives = 38/87 (43%), Gaps = 1/87 (1%)
 Frame = -1

Query: 353 ITYLHDQSPLLAGVIFTRCAFNILIKRRGSAKYPHF-VNKKTST*LYVIDKNIK*FLILK 177
           +TYLH     +           +L  R    K P F +++K     Y+        LI  
Sbjct: 546 VTYLHRILNAIKETYSGYLLLTVLCMRLNIIKTPSFTLDEKIQESYYMAHYRP---LINL 602

Query: 176 SE*NRILQTKISCFMKNLRNKNTDYII 96
            +   +L+++  C +KNL+N +TD +I
Sbjct: 603 RQPKPLLRSEADCIIKNLQNFSTDDLI 629


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,439,753
Number of Sequences: 5004
Number of extensions: 48049
Number of successful extensions: 144
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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