Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0518
(713 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g01760.1 68418.m00095 VHS domain-containing protein / GAT dom... 66 2e-11
At2g38410.1 68415.m04718 VHS domain-containing protein / GAT dom... 66 3e-11
At5g16880.3 68418.m01977 VHS domain-containing protein / GAT dom... 65 4e-11
At5g16880.2 68418.m01979 VHS domain-containing protein / GAT dom... 65 4e-11
At5g16880.1 68418.m01978 VHS domain-containing protein / GAT dom... 65 4e-11
At3g08790.1 68416.m01021 VHS domain-containing protein / GAT dom... 60 1e-09
At5g63640.1 68418.m07990 VHS domain-containing protein / GAT dom... 53 2e-07
At1g21380.1 68414.m02675 VHS domain-containing protein / GAT dom... 49 3e-06
At1g06210.2 68414.m00653 VHS domain-containing protein / GAT dom... 49 3e-06
At1g06210.1 68414.m00654 VHS domain-containing protein / GAT dom... 49 3e-06
At1g76970.1 68414.m08962 VHS domain-containing protein / GAT dom... 46 2e-05
At4g32760.1 68417.m04661 VHS domain-containing protein / GAT dom... 33 0.14
At5g44570.1 68418.m05461 hypothetical protein 28 7.1
At3g06180.1 68416.m00710 expressed protein 28 7.1
>At5g01760.1 68418.m00095 VHS domain-containing protein / GAT
domain-containing protein weak similarity to Hrs [Rattus
norvegicus] GI:8547026; contains Pfam profiles PF00790:
VHS domain, PF03127: GAT domain
Length = 542
Score = 66.1 bits (154), Expect = 2e-11
Identities = 30/87 (34%), Positives = 50/87 (57%)
Frame = +3
Query: 288 VERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAVMRRLAHPDPHVQVHAATLLDAC 467
V++ATSE + +W +I+ ICD ++ K+ ++AV RRL H VQ+ TLL+A
Sbjct: 26 VDKATSELLRTPDWTIIIAICDSLNSNRWQCKDAIKAVKRRLQHKSSRVQLLTLTLLEAM 85
Query: 468 VANCGRFFHLEVASRDFETEFRRLLSR 548
+ NCG F H +A + + +L+ +
Sbjct: 86 LKNCGDFVHSHIAEKHLLEDMVKLVRK 112
>At2g38410.1 68415.m04718 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate HRS
isoform 2 [Homo sapiens] GI:9022389; contains Pfam
profiles PF00790: VHS domain, PF03127: GAT domain
Length = 671
Score = 65.7 bits (153), Expect = 3e-11
Identities = 31/87 (35%), Positives = 50/87 (57%)
Frame = +3
Query: 288 VERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAVMRRLAHPDPHVQVHAATLLDAC 467
V++ATS+ +W MEICD + AK+ ++AV +RL H VQ+ A TLL+
Sbjct: 12 VDKATSDLLLGPDWTTNMEICDSVNSLHWQAKDVVKAVKKRLQHKSSRVQLLALTLLETL 71
Query: 468 VANCGRFFHLEVASRDFETEFRRLLSR 548
V NCG + H +VA ++ E +++ +
Sbjct: 72 VKNCGDYLHHQVAEKNILGEMVKIVKK 98
>At5g16880.3 68418.m01977 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate [Homo
sapiens] GI:2731383; contains Pfam profiles PF00790: VHS
domain, PF03127: GAT domain
Length = 297
Score = 65.3 bits (152), Expect = 4e-11
Identities = 38/114 (33%), Positives = 59/114 (51%)
Frame = +3
Query: 222 SSRCFFVNKMGIFGTSSPFDQDVERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAV 401
SS F V ++ F +P D+ VE AT+EN +W + +EICD + ++ E +R +
Sbjct: 32 SSMSFKVKEL--FQGPNPTDKIVEDATTENLEEPDWDMNLEICDMINQETINSVELIRGI 89
Query: 402 MRRLAHPDPHVQVHAATLLDACVANCGRFFHLEVASRDFETEFRRLLSRAQPPV 563
+R+ P +Q A LL+ CV NC + F EVA+ E +L+ Q V
Sbjct: 90 KKRIMMKQPRIQYLALVLLETCVKNCEKAFS-EVAAERVLDEMVKLIDDPQTVV 142
>At5g16880.2 68418.m01979 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate [Homo
sapiens] GI:2731383; contains Pfam profiles PF00790: VHS
domain, PF03127: GAT domain
Length = 407
Score = 65.3 bits (152), Expect = 4e-11
Identities = 38/114 (33%), Positives = 59/114 (51%)
Frame = +3
Query: 222 SSRCFFVNKMGIFGTSSPFDQDVERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAV 401
SS F V ++ F +P D+ VE AT+EN +W + +EICD + ++ E +R +
Sbjct: 32 SSMSFKVKEL--FQGPNPTDKIVEDATTENLEEPDWDMNLEICDMINQETINSVELIRGI 89
Query: 402 MRRLAHPDPHVQVHAATLLDACVANCGRFFHLEVASRDFETEFRRLLSRAQPPV 563
+R+ P +Q A LL+ CV NC + F EVA+ E +L+ Q V
Sbjct: 90 KKRIMMKQPRIQYLALVLLETCVKNCEKAFS-EVAAERVLDEMVKLIDDPQTVV 142
>At5g16880.1 68418.m01978 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate [Homo
sapiens] GI:2731383; contains Pfam profiles PF00790: VHS
domain, PF03127: GAT domain
Length = 407
Score = 65.3 bits (152), Expect = 4e-11
Identities = 38/114 (33%), Positives = 59/114 (51%)
Frame = +3
Query: 222 SSRCFFVNKMGIFGTSSPFDQDVERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAV 401
SS F V ++ F +P D+ VE AT+EN +W + +EICD + ++ E +R +
Sbjct: 32 SSMSFKVKEL--FQGPNPTDKIVEDATTENLEEPDWDMNLEICDMINQETINSVELIRGI 89
Query: 402 MRRLAHPDPHVQVHAATLLDACVANCGRFFHLEVASRDFETEFRRLLSRAQPPV 563
+R+ P +Q A LL+ CV NC + F EVA+ E +L+ Q V
Sbjct: 90 KKRIMMKQPRIQYLALVLLETCVKNCEKAFS-EVAAERVLDEMVKLIDDPQTVV 142
>At3g08790.1 68416.m01021 VHS domain-containing protein / GAT
domain-containing protein weak similarity to
HGF-regulated tyrosine kinase substrate [Mus musculus]
GI:1089781; contains Pfam profiles PF00790: VHS domain,
PF03127: GAT domain
Length = 607
Score = 60.5 bits (140), Expect = 1e-09
Identities = 27/87 (31%), Positives = 45/87 (51%)
Frame = +3
Query: 288 VERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAVMRRLAHPDPHVQVHAATLLDAC 467
V+RATS+ +W + +EICD +E + + +RL VQ+ A TLL+
Sbjct: 6 VDRATSDMLIGPDWAMNLEICDMLNHEPGQTREVVSGIKKRLTSRTSKVQLLALTLLETI 65
Query: 468 VANCGRFFHLEVASRDFETEFRRLLSR 548
+ NCG H++VA +D + ++ R
Sbjct: 66 ITNCGELIHMQVAEKDILHKMVKMAKR 92
>At5g63640.1 68418.m07990 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate HRS
isoform 2 [Homo sapiens] GI:9022389; contains Pfam
profiles PF00790: VHS domain, PF03127: GAT domain
Length = 447
Score = 52.8 bits (121), Expect = 2e-07
Identities = 24/72 (33%), Positives = 39/72 (54%)
Frame = +3
Query: 288 VERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAVMRRLAHPDPHVQVHAATLLDAC 467
V ATSE +W +EIC+ A AK+ ++A+ +RL +P+ Q++A LL+
Sbjct: 6 VSSATSEKLADVDWAKNIEICELAARDERQAKDVIKAIKKRLGSKNPNTQLYAVQLLEML 65
Query: 468 VANCGRFFHLEV 503
+ N G H +V
Sbjct: 66 MNNIGENIHKQV 77
>At1g21380.1 68414.m02675 VHS domain-containing protein / GAT
domain-containing protein weak similarity to Hrs [Rattus
norvegicus] GI:8547026; contains Pfam profiles PF00790:
VHS domain, PF03127: GAT domain
Length = 506
Score = 49.2 bits (112), Expect = 3e-06
Identities = 22/86 (25%), Positives = 43/86 (50%)
Frame = +3
Query: 291 ERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAVMRRLAHPDPHVQVHAATLLDACV 470
ERAT++ +W + +E+CD + AKE ++ + +RL + VQ+ A L+
Sbjct: 10 ERATNDMLIGPDWAINIELCDIINMEPSQAKEAVKVLKKRLGSKNSKVQILALYALETLS 69
Query: 471 ANCGRFFHLEVASRDFETEFRRLLSR 548
NCG + + RD + +++ +
Sbjct: 70 KNCGESVYQLIVDRDILPDMVKIVKK 95
>At1g06210.2 68414.m00653 VHS domain-containing protein / GAT
domain-containing protein weak similarity to SP|Q9UJY5
ADP-ribosylation factor binding protein GGA1 {Homo
sapiens}; contains Pfam profiles PF00790: VHS domain,
PF03127: GAT domain
Length = 279
Score = 49.2 bits (112), Expect = 3e-06
Identities = 28/89 (31%), Positives = 42/89 (47%)
Frame = +3
Query: 288 VERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAVMRRLAHPDPHVQVHAATLLDAC 467
V+ AT E WG+ M IC + + E +RA+ R+++ P Q + LL+AC
Sbjct: 42 VDEATLETLEEPNWGMNMRICAQINNDEFNGTEIVRAIKRKISGKSPVSQRLSLELLEAC 101
Query: 468 VANCGRFFHLEVASRDFETEFRRLLSRAQ 554
NC + F EVAS E L+ +
Sbjct: 102 AMNCEKVFS-EVASEKVLDEMVWLIKNGE 129
>At1g06210.1 68414.m00654 VHS domain-containing protein / GAT
domain-containing protein weak similarity to SP|Q9UJY5
ADP-ribosylation factor binding protein GGA1 {Homo
sapiens}; contains Pfam profiles PF00790: VHS domain,
PF03127: GAT domain
Length = 383
Score = 49.2 bits (112), Expect = 3e-06
Identities = 28/89 (31%), Positives = 42/89 (47%)
Frame = +3
Query: 288 VERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAVMRRLAHPDPHVQVHAATLLDAC 467
V+ AT E WG+ M IC + + E +RA+ R+++ P Q + LL+AC
Sbjct: 42 VDEATLETLEEPNWGMNMRICAQINNDEFNGTEIVRAIKRKISGKSPVSQRLSLELLEAC 101
Query: 468 VANCGRFFHLEVASRDFETEFRRLLSRAQ 554
NC + F EVAS E L+ +
Sbjct: 102 AMNCEKVFS-EVASEKVLDEMVWLIKNGE 129
>At1g76970.1 68414.m08962 VHS domain-containing protein / GAT
domain-containing protein weak similarity to
HGF-regulated tyrosine kinase substrate [Mus musculus]
GI:1089781; contains Pfam profiles PF00790: VHS domain,
PF03127: GAT domain
Length = 446
Score = 46.4 bits (105), Expect = 2e-05
Identities = 21/86 (24%), Positives = 42/86 (48%)
Frame = +3
Query: 291 ERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAVMRRLAHPDPHVQVHAATLLDACV 470
ERAT++ +W + +E+CD + AKE ++ + +RL + VQ+ A L+
Sbjct: 10 ERATNDMLIGPDWAINIELCDLINMDPSQAKEAVKVLKKRLGSKNSKVQILALYALETLS 69
Query: 471 ANCGRFFHLEVASRDFETEFRRLLSR 548
NCG + + R + +++ +
Sbjct: 70 KNCGENVYQLIIDRGLLNDMVKIVKK 95
>At4g32760.1 68417.m04661 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate HRS
isoform 2 [Homo sapiens] GI:9022389; contains Pfam
profiles PF00790: VHS domain, PF03127: GAT domain
Length = 838
Score = 33.5 bits (73), Expect = 0.14
Identities = 29/103 (28%), Positives = 43/103 (41%), Gaps = 16/103 (15%)
Frame = +3
Query: 288 VERATSENNTSEEWGLIMEICDR-----AGASSTSAKECLRAVM----------RRLAHP 422
VERATSE +W + +EICD A S S + C + R +
Sbjct: 6 VERATSEMLIGPDWAMNLEICDMLNSDPAYVSLCSLRFCFCCYLSLSLYLFERERERVNS 65
Query: 423 DPHVQVHAAT-LLDACVANCGRFFHLEVASRDFETEFRRLLSR 548
++ T LL+ V NCG H+ VA + E R++ +
Sbjct: 66 KMEIKFRFFTRLLETIVKNCGDMVHMHVAEKGVIHEMVRIVKK 108
>At5g44570.1 68418.m05461 hypothetical protein
Length = 133
Score = 27.9 bits (59), Expect = 7.1
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +1
Query: 487 SSTWKSPRETSRPSSGACCLARSLLSPLVSAPCSASGP 600
S+T K + +S S+ A C A + +P A CS + P
Sbjct: 37 STTIKKSKRSSIQSTDAACAAATTTAPAPDAECSHTSP 74
>At3g06180.1 68416.m00710 expressed protein
Length = 241
Score = 27.9 bits (59), Expect = 7.1
Identities = 17/55 (30%), Positives = 26/55 (47%)
Frame = +3
Query: 261 GTSSPFDQDVERATSENNTSEEWGLIMEICDRAGASSTSAKECLRAVMRRLAHPD 425
G + D++ T E S I + + G+ S ECLRA +RR+A P+
Sbjct: 142 GLKKAMEFDLQLQTEECVKSGATKEIDRLPWKGGSESNPDYECLRAELRRMAPPN 196
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,697,393
Number of Sequences: 28952
Number of extensions: 252087
Number of successful extensions: 713
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 689
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 713
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1545769616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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