Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0514
(618 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 340 7e-94
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 335 2e-92
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 282 2e-76
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 268 3e-72
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 165 2e-41
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 113 1e-25
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 101 6e-22
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 70 1e-12
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 5e-04
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.75
SB_34580| Best HMM Match : Gp-FAR-1 (HMM E-Value=0.88) 29 2.3
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.3
SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37) 29 3.0
SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9) 29 3.0
SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.0
SB_32880| Best HMM Match : rve (HMM E-Value=3.4e-05) 28 7.0
SB_14214| Best HMM Match : AmoC (HMM E-Value=3.2) 28 7.0
SB_43817| Best HMM Match : WD40 (HMM E-Value=1e-13) 27 9.2
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 340 bits (835), Expect = 7e-94
Identities = 159/188 (84%), Positives = 173/188 (92%)
Frame = +1
Query: 55 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 234
MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 235 MNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVS 414
MNP NT+FDAKRLIGRKF+D V DM HWPF V+ +G +PKI+V +KGE K+FFPEE+S
Sbjct: 61 MNPENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEIS 120
Query: 415 SMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAA 594
SMVLTKMKETAEAYLG V +AV+TVPAYF+DSQRQATKDAGTI+GLNVLRIINEPTAAA
Sbjct: 121 SMVLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATKDAGTIAGLNVLRIINEPTAAA 180
Query: 595 IAYGLDKK 618
IAYGLDKK
Sbjct: 181 IAYGLDKK 188
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 335 bits (823), Expect = 2e-92
Identities = 156/187 (83%), Positives = 174/187 (93%)
Frame = +1
Query: 55 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 234
M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 235 MNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVS 414
+NP NTIFDAKRLIGR+++DATVQ+DMK WPF+++SD KPKI+V YKGE KTF EE+S
Sbjct: 61 LNPENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEIS 120
Query: 415 SMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAA 594
SMVL KMKETAEAYLG+ V +AVITVPAYFNDSQRQATKDAGTI+GLNVLR+INEPTAAA
Sbjct: 121 SMVLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATKDAGTIAGLNVLRVINEPTAAA 180
Query: 595 IAYGLDK 615
+AYGLDK
Sbjct: 181 LAYGLDK 187
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 282 bits (691), Expect = 2e-76
Identities = 134/183 (73%), Positives = 157/183 (85%), Gaps = 1/183 (0%)
Frame = +1
Query: 73 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNPNN 249
+GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP N
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763
Query: 250 TIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLT 429
T+FDAKRLIGR ++D +VQ D+K +PF+VV KP ++V K E KTF EE+S+MVLT
Sbjct: 764 TVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAMVLT 823
Query: 430 KMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGL 609
KMKETAEAYLGK V +AV+TVPAYFND+QRQATKDAG I+GL V+RIINEPTAAAIAYG+
Sbjct: 824 KMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGAIAGLTVMRIINEPTAAAIAYGM 883
Query: 610 DKK 618
DKK
Sbjct: 884 DKK 886
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 268 bits (656), Expect = 3e-72
Identities = 136/187 (72%), Positives = 147/187 (78%), Gaps = 25/187 (13%)
Frame = +1
Query: 55 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 234
M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 235 MNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEV- 411
MNP NTIFDAKRLIGR+F+D V+ DMKHW FEVV++ G+PK+KV YKGE KTFF EEV
Sbjct: 61 MNPTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVV 120
Query: 412 ------------------------SSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQR 519
SSMVL KMKETAEAYLG V +AV+TVPAYFNDSQR
Sbjct: 121 NEAGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQR 180
Query: 520 QATKDAG 540
QATKDAG
Sbjct: 181 QATKDAG 187
Score = 127 bits (307), Expect = 6e-30
Identities = 62/71 (87%), Positives = 66/71 (92%)
Frame = +1
Query: 406 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPT 585
++SSMVL KMKETAEAYLG V +AV+TVPAYFNDSQRQATKDAG ISGLNVLRIINEPT
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAGVISGLNVLRIINEPT 270
Query: 586 AAAIAYGLDKK 618
AAAIAYGLDKK
Sbjct: 271 AAAIAYGLDKK 281
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 165 bits (402), Expect = 2e-41
Identities = 86/189 (45%), Positives = 118/189 (62%), Gaps = 3/189 (1%)
Frame = +1
Query: 61 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 234
K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 235 MNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDG-GKPKIKVAYKGEDKTFFPEEV 411
+NP NTI+DAKR IG+ F + K + F V +D G V KG PEE+
Sbjct: 90 INPKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEI 149
Query: 412 SSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAA 591
V+ K++ AE L TV AV++VPA F+ QR AT A +++G+ VLR+INEPTAA
Sbjct: 150 GGYVIKKLRTMAERNLTTTVTKAVMSVPAEFDIKQRNATVKAASLAGITVLRVINEPTAA 209
Query: 592 AIAYGLDKK 618
A+AYGL KK
Sbjct: 210 ALAYGLHKK 218
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 113 bits (271), Expect = 1e-25
Identities = 56/128 (43%), Positives = 81/128 (63%), Gaps = 1/128 (0%)
Frame = +1
Query: 238 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVS-DGGKPKIKVAYKGEDKTFFPEEVS 414
N NTI KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V
Sbjct: 118 NLKNTISQFKRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVM 177
Query: 415 SMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAA 594
+M+ T++K TAE L V + VI+VP+Y+ D QR+ DA +GLN LR++N+ TA +
Sbjct: 178 AMLFTRLKTTAEIALKTKVTDCVISVPSYYTDRQRRCMLDASATAGLNCLRLMNDTTAVS 237
Query: 595 IAYGLDKK 618
+AYG+ K+
Sbjct: 238 LAYGIYKQ 245
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +1
Query: 73 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 228
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 101 bits (241), Expect = 6e-22
Identities = 59/109 (54%), Positives = 74/109 (67%), Gaps = 24/109 (22%)
Frame = +1
Query: 361 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAG 540
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA+FND+QRQATKDAG
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATKDAG 61
Query: 541 TISGLN------------------------VLRIINEPTAAAIAYGLDK 615
TI+GL+ VLRIINEPTAAAIAYGLDK
Sbjct: 62 TIAGLDVLSRTASERPPSRFRRRRPHGRSRVLRIINEPTAAAIAYGLDK 110
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 70.1 bits (164), Expect = 1e-12
Identities = 34/85 (40%), Positives = 52/85 (61%)
Frame = +1
Query: 355 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKD 534
P +K Y+ D TF PEE+ M+L + E + +++ V+TVP +FN ++R+A
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFNQAERRALLR 126
Query: 535 AGTISGLNVLRIINEPTAAAIAYGL 609
A + GLNVL+I+N TA A+ YGL
Sbjct: 127 AAELVGLNVLQIMNSNTAVALNYGL 151
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 41.5 bits (93), Expect = 5e-04
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = +1
Query: 124 GKVEIIANDQGNRTTPSYVAFTDTERLI 207
G IIAND G+R TP+ VAF+DTE+LI
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLI 110
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.75
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +1
Query: 133 EIIANDQGNRTTPSYVAFT-DTERL 204
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_34580| Best HMM Match : Gp-FAR-1 (HMM E-Value=0.88)
Length = 953
Score = 29.5 bits (63), Expect = 2.3
Identities = 16/60 (26%), Positives = 27/60 (45%), Gaps = 3/60 (5%)
Frame = +3
Query: 141 RQRPGQQDHSVLCCVHRHRASHRRCRQEPGG---DEPQQHNIRCQTSHRT*VRRCYCASR 311
RQ + +++ H R +R+ ++ GG +EP + R T T C+CA R
Sbjct: 267 RQSAPSRSMAIINKTHGKRHGYRKLSRDCGGRRPNEPYERTARVHTILSTDTETCFCAKR 326
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 2.3
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -3
Query: 241 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 143
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37)
Length = 420
Score = 29.1 bits (62), Expect = 3.0
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = -3
Query: 259 RILCCWGSSPPGSWRH 212
R++CC G SP WRH
Sbjct: 395 RVVCCQGDSPSPRWRH 410
>SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9)
Length = 340
Score = 29.1 bits (62), Expect = 3.0
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = -3
Query: 259 RILCCWGSSPPGSWRH 212
R++CC G SP WRH
Sbjct: 170 RVVCCQGDSPSPRWRH 185
>SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1506
Score = 28.7 bits (61), Expect = 4.0
Identities = 20/68 (29%), Positives = 32/68 (47%)
Frame = -2
Query: 449 SAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMSACTVASSNLRPMR 270
S F+F ST + +S +KV+ SPL + + P+ G S C V NL
Sbjct: 1108 SPAKFVFTSTGIVRASTRKVVPSPLRRSKVPPTVPAQRLFNGDRVESICNV--QNLSRCP 1165
Query: 269 RLASNIVL 246
++++ VL
Sbjct: 1166 KISNATVL 1173
>SB_32880| Best HMM Match : rve (HMM E-Value=3.4e-05)
Length = 1264
Score = 27.9 bits (59), Expect = 7.0
Identities = 15/50 (30%), Positives = 23/50 (46%), Gaps = 3/50 (6%)
Frame = +3
Query: 186 HRHRASHRRCRQEPGG---DEPQQHNIRCQTSHRT*VRRCYCASRHEALA 326
H R +R+ + GG +EP + R T T C+CA R+ + A
Sbjct: 421 HGERHGYRKLSRVCGGRRPNEPYERTARVHTILSTDTETCFCAKRYGSAA 470
>SB_14214| Best HMM Match : AmoC (HMM E-Value=3.2)
Length = 451
Score = 27.9 bits (59), Expect = 7.0
Identities = 14/49 (28%), Positives = 22/49 (44%)
Frame = -2
Query: 323 QCFMSACTVASSNLRPMRRLASNIVLLGFIATWFLAASPMRRSVSVNAT 177
QCF+S C + S + + R+ L TW + + M S +AT
Sbjct: 169 QCFLSVCDLCCSLMTQIPRMRHTWDFLEHAPTWISSQTSMSFSAITDAT 217
>SB_43817| Best HMM Match : WD40 (HMM E-Value=1e-13)
Length = 822
Score = 27.5 bits (58), Expect = 9.2
Identities = 23/71 (32%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
Frame = -2
Query: 428 VSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMS-ACTVASSNLRPMRRLASNI 252
VST+++T G +L+ T LG+ + T S S T+ S +L PMRR +
Sbjct: 751 VSTVDVTLDGLYILAGT--TTGNLGVLNASTRSYTTLMRSHTSTITSMDLDPMRRHMGTV 808
Query: 251 VLLGFIATWFL 219
G I W L
Sbjct: 809 SADGTIRIWDL 819
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,814,476
Number of Sequences: 59808
Number of extensions: 476834
Number of successful extensions: 1337
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1235
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1333
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1524174750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -