BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0503 (696 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schi... 134 1e-32 SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 27 2.6 SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 3.4 >SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schizosaccharomyces pombe|chr 2|||Manual Length = 965 Score = 134 bits (324), Expect = 1e-32 Identities = 69/200 (34%), Positives = 117/200 (58%), Gaps = 4/200 (2%) Frame = +1 Query: 55 ITSAAGIISLLDEPMSEVKEFALKRLDNIVDEFWPXXXXXXXXXXXLHEDKIFSQHQLAA 234 ITSA G+++LLDE E++ AL ++ +D+ WP ++ED F + +LAA Sbjct: 12 ITSAGGLMALLDEQERELQVHALLKIYEFIDQLWPEISDDVTKIEVMYEDHSFPERELAA 71 Query: 235 LVASKVYYHLGAFEDSLTYALGAGDLFDVNARNEYVDTTIAKAIDFYTQKRKALFVDSSA 414 LV SKVYY+LG ++++L +AL +G F + ++Y +T I K ID + K L+ + A Sbjct: 72 LVVSKVYYYLGEYDEALLFALSSGPKFLHDKNSDYKETLIFKCIDMFIHKSAELYKNPKA 131 Query: 415 ELIDHRLEDIVNRMFQRCLEDGQYRQALGLSLETRRMDIFEESIM--KSDDI-PGMLQYA 585 + +D RL +V +FQ+C ++R LG+++E R+DI E + K D+ P +L+ A Sbjct: 132 DPLDERLSGVVEGIFQKCYAKNEWRHVLGIAIEAHRLDIIEYILNADKETDLKPYVLELA 191 Query: 586 FTVAMSL-LQNKASAVLYSA 642 TV + + +N+ +L S+ Sbjct: 192 MTVVLDIEFRNRLLRLLLSS 211 >SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 547 Score = 27.1 bits (57), Expect = 2.6 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = +1 Query: 295 LGAGDLFDVNARNEYVDTTIAKAIDFYTQK 384 +G G D +A+ YVDT A++ TQ+ Sbjct: 280 IGCGRYHDAHAKQHYVDTAHCYAMELETQR 309 >SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces pombe|chr 2|||Manual Length = 1485 Score = 26.6 bits (56), Expect = 3.4 Identities = 12/40 (30%), Positives = 20/40 (50%) Frame = -1 Query: 180 LDRFRYFWPKFIDDVVQPLQSEFFNFRHGLIEQGNNPRGR 61 LD F + W K +DD+ + + E + R + PRG+ Sbjct: 1195 LDAFEHAWNKVMDDIQREMLEEEQSSRDFVNRTKKKPRGK 1234 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,792,373 Number of Sequences: 5004 Number of extensions: 56610 Number of successful extensions: 154 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 152 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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