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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0503
         (696 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schi...   134   1e-32
SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    27   2.6  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    27   3.4  

>SPBC17D11.07c |rpn2||19S proteasome regulatory subunit
           Rpn2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 965

 Score =  134 bits (324), Expect = 1e-32
 Identities = 69/200 (34%), Positives = 117/200 (58%), Gaps = 4/200 (2%)
 Frame = +1

Query: 55  ITSAAGIISLLDEPMSEVKEFALKRLDNIVDEFWPXXXXXXXXXXXLHEDKIFSQHQLAA 234
           ITSA G+++LLDE   E++  AL ++   +D+ WP           ++ED  F + +LAA
Sbjct: 12  ITSAGGLMALLDEQERELQVHALLKIYEFIDQLWPEISDDVTKIEVMYEDHSFPERELAA 71

Query: 235 LVASKVYYHLGAFEDSLTYALGAGDLFDVNARNEYVDTTIAKAIDFYTQKRKALFVDSSA 414
           LV SKVYY+LG ++++L +AL +G  F  +  ++Y +T I K ID +  K   L+ +  A
Sbjct: 72  LVVSKVYYYLGEYDEALLFALSSGPKFLHDKNSDYKETLIFKCIDMFIHKSAELYKNPKA 131

Query: 415 ELIDHRLEDIVNRMFQRCLEDGQYRQALGLSLETRRMDIFEESIM--KSDDI-PGMLQYA 585
           + +D RL  +V  +FQ+C    ++R  LG+++E  R+DI E  +   K  D+ P +L+ A
Sbjct: 132 DPLDERLSGVVEGIFQKCYAKNEWRHVLGIAIEAHRLDIIEYILNADKETDLKPYVLELA 191

Query: 586 FTVAMSL-LQNKASAVLYSA 642
            TV + +  +N+   +L S+
Sbjct: 192 MTVVLDIEFRNRLLRLLLSS 211


>SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 547

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 11/30 (36%), Positives = 17/30 (56%)
 Frame = +1

Query: 295 LGAGDLFDVNARNEYVDTTIAKAIDFYTQK 384
           +G G   D +A+  YVDT    A++  TQ+
Sbjct: 280 IGCGRYHDAHAKQHYVDTAHCYAMELETQR 309


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1485

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 12/40 (30%), Positives = 20/40 (50%)
 Frame = -1

Query: 180  LDRFRYFWPKFIDDVVQPLQSEFFNFRHGLIEQGNNPRGR 61
            LD F + W K +DD+ + +  E  + R  +      PRG+
Sbjct: 1195 LDAFEHAWNKVMDDIQREMLEEEQSSRDFVNRTKKKPRGK 1234


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,792,373
Number of Sequences: 5004
Number of extensions: 56610
Number of successful extensions: 154
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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