Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0503
(696 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g32730.1 68415.m04005 26S proteasome regulatory subunit, puta... 175 2e-44
At1g04810.1 68414.m00477 26S proteasome regulatory subunit, puta... 171 3e-43
At3g52690.1 68416.m05804 hypothetical protein predicted proteins... 32 0.32
At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-contain... 31 0.73
At3g12150.1 68416.m01514 expressed protein 31 0.73
At4g29800.1 68417.m04243 patatin-related low similarity to patat... 28 5.1
At1g15940.1 68414.m01913 expressed protein similar To androgen-i... 28 5.1
At1g08620.1 68414.m00955 transcription factor jumonji (jmj) fami... 28 5.1
At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 28 6.8
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 28 6.8
At4g39910.1 68417.m05653 ubiquitin-specific protease 3 (UBP3) id... 28 6.8
At1g65920.1 68414.m07480 regulator of chromosome condensation (R... 28 6.8
>At2g32730.1 68415.m04005 26S proteasome regulatory subunit,
putative contains similarity to 26S proteasome
regulatory subunit S1 SP:O88761, GI:3288594 from [Rattus
norvegicus]
Length = 1004
Score = 175 bits (427), Expect = 2e-44
Identities = 86/190 (45%), Positives = 124/190 (65%), Gaps = 2/190 (1%)
Frame = +1
Query: 55 ITSAAGIISLLDEPMSEVKEFALKRLDNIVDEFWPXXXXXXXXXXXLHEDKIFSQHQ--L 228
++SA G++++L+EP +K AL L+N+VD+FWP L+ED+ F HQ L
Sbjct: 6 VSSAGGLLAMLNEPHPVLKLHALSNLNNLVDQFWPEISTSVPIIESLYEDEEFDLHQRQL 65
Query: 229 AALVASKVYYHLGAFEDSLTYALGAGDLFDVNARNEYVDTTIAKAIDFYTQKRKALFVDS 408
AAL+ SKV+Y+LG DSL+YALGAG LFDV+ ++YV T +AKAID Y R +
Sbjct: 66 AALLVSKVFYYLGELNDSLSYALGAGPLFDVSEDSDYVHTLLAKAIDEYASLRSKAVESN 125
Query: 409 SAELIDHRLEDIVNRMFQRCLEDGQYRQALGLSLETRRMDIFEESIMKSDDIPGMLQYAF 588
ID RLE IV RM +C+ DG+Y+QA+G+++E RR+D EE+I+KSD++ G L Y
Sbjct: 126 EMVDIDPRLEAIVERMLGKCISDGKYQQAMGIAIECRRLDKLEEAIIKSDNVQGTLSYCI 185
Query: 589 TVAMSLLQNK 618
V+ S + +
Sbjct: 186 NVSHSFVNRR 195
>At1g04810.1 68414.m00477 26S proteasome regulatory subunit,
putative contains similarity to 26S proteasome
regulatory subunit S1 SP:O88761, GI:3288594 from [Rattus
norvegicus]
Length = 1001
Score = 171 bits (417), Expect = 3e-43
Identities = 84/190 (44%), Positives = 123/190 (64%), Gaps = 2/190 (1%)
Frame = +1
Query: 55 ITSAAGIISLLDEPMSEVKEFALKRLDNIVDEFWPXXXXXXXXXXXLHEDKIFSQHQ--L 228
++SA G++++L+EP +K AL L +VD+FWP L+ED+ F QHQ L
Sbjct: 6 VSSAGGLLAMLNEPHPSLKLHALSYLIRLVDQFWPEISTSVPIIESLYEDEEFDQHQRQL 65
Query: 229 AALVASKVYYHLGAFEDSLTYALGAGDLFDVNARNEYVDTTIAKAIDFYTQKRKALFVDS 408
AAL+ASKV+Y+LG DSL+YALGAG LFDV+ ++Y+ T ++KAID Y R S
Sbjct: 66 AALLASKVFYYLGELNDSLSYALGAGSLFDVSEDSDYIHTLLSKAIDEYAILRSKAVESS 125
Query: 409 SAELIDHRLEDIVNRMFQRCLEDGQYRQALGLSLETRRMDIFEESIMKSDDIPGMLQYAF 588
ID RL IV RM +C+ DG+Y+QA+G+++E RR+D EE+I+KS+++ G L Y
Sbjct: 126 EVVEIDPRLVAIVERMLDKCITDGKYQQAMGIAIECRRLDKLEEAIIKSENVQGTLSYCI 185
Query: 589 TVAMSLLQNK 618
V+ S + +
Sbjct: 186 NVSHSFVNQR 195
>At3g52690.1 68416.m05804 hypothetical protein predicted proteins,
Arabidopsis thaliana
Length = 299
Score = 32.3 bits (70), Expect = 0.32
Identities = 27/102 (26%), Positives = 54/102 (52%), Gaps = 1/102 (0%)
Frame = -1
Query: 663 PYIKSLR-SGVQYCGSLILEETHCYCESVLKHSWYIIRFHNRLLENIHTARLQ**AKRLS 487
P +K L+ G+ +CG ++E E+ +++ +YI + ++L ++ + AKR
Sbjct: 96 PTLKYLKIKGLSHCGFCLIENAPVLVEAKIRNLFYI--YKEKILGSLKS------AKR-- 145
Query: 486 VLSIFKAPLEHAIHDVFKTVIN*FSRTIHKESLSLLSVKVDS 361
LS+ +PLE I +F +++ T +E +LL + +DS
Sbjct: 146 -LSLDLSPLECPIDSIFYQLVHLEIYTCKEEWWNLLKLMLDS 186
>At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to glucocorticoid receptor DNA binding factor
1 [Canis familiaris] GI:23266717; contains Pfam profiles
PF00169: PH domain, PF00620: RhoGAP domain
Length = 827
Score = 31.1 bits (67), Expect = 0.73
Identities = 21/61 (34%), Positives = 32/61 (52%), Gaps = 2/61 (3%)
Frame = +1
Query: 403 DSSA--ELIDHRLEDIVNRMFQRCLEDGQYRQALGLSLETRRMDIFEESIMKSDDIPGML 576
DSS EL+ RLE + + + QR ++ + AL SLE R+ + E + D+ G L
Sbjct: 557 DSSGNDELLIQRLEHMKDELRQRIAKEAKGNAALQASLERRKQALHERRLALEQDV-GRL 615
Query: 577 Q 579
Q
Sbjct: 616 Q 616
>At3g12150.1 68416.m01514 expressed protein
Length = 363
Score = 31.1 bits (67), Expect = 0.73
Identities = 21/55 (38%), Positives = 27/55 (49%), Gaps = 10/55 (18%)
Frame = -3
Query: 499 QALVCIVHLQGTSGTCDSRCLQDGDQLVQQN----------YPQREPFSFECKSR 365
Q + C+VHL GT R L+ G LV+QN Y QR PF +C +R
Sbjct: 120 QKMACVVHLAGTGDHTYDRRLRLGGPLVKQNIATMVLESPFYGQRRPF-LQCGAR 173
>At4g29800.1 68417.m04243 patatin-related low similarity to patatin
precursor [Solanum brevidens][GI:563125]; contains
Patatin domain PF01743
Length = 525
Score = 28.3 bits (60), Expect = 5.1
Identities = 18/51 (35%), Positives = 24/51 (47%)
Frame = +1
Query: 274 EDSLTYALGAGDLFDVNARNEYVDTTIAKAIDFYTQKRKALFVDSSAELID 426
ED L +LG G LF+VN E V K + + + D SAE +D
Sbjct: 355 EDLLVLSLGTGQLFEVNYDYEQVKNWRVKE---WARPMARISGDGSAEFVD 402
>At1g15940.1 68414.m01913 expressed protein similar To
androgen-induced prostate proliferative shutoff
associated protein (GI:4559410) [Homo sapiens]
Length = 990
Score = 28.3 bits (60), Expect = 5.1
Identities = 23/81 (28%), Positives = 42/81 (51%), Gaps = 3/81 (3%)
Frame = +1
Query: 400 VDSSAELIDHRLEDIVNRMFQRCLE--DGQYRQALGLSLETRRMDIFEESIMKSDDIP-G 570
V SS ++D +D+V MFQR L+ + Q + +S+ET + + +E S+++P
Sbjct: 140 VRSSLVMLDLECDDLVLEMFQRFLKIIRPDHPQLVLVSMETIMITVIDE----SEEVPMD 195
Query: 571 MLQYAFTVAMSLLQNKASAVL 633
+L+ T Q+ + A L
Sbjct: 196 LLEILLTTVKKDSQDVSPAAL 216
>At1g08620.1 68414.m00955 transcription factor jumonji (jmj) family
protein / zinc finger (C5HC2 type) family protein
contains Pfam domains, PF02375: jmjN domain, PF02373:
jmjC domain and PF02928: C5HC2 zinc finger
Length = 1183
Score = 28.3 bits (60), Expect = 5.1
Identities = 18/64 (28%), Positives = 31/64 (48%)
Frame = -3
Query: 268 LLNGSTLYWLLMRLADAGRKFCLRAGFLFSR*IQIFLAKIHRRCCPTSSERIL*LQTWAH 89
+L+ + + + + + DAGR L +L S ++F+ RC ER+ T H
Sbjct: 990 ILDPTNMCFYISEILDAGRNSPLFMVYLESNPSEVFVHMSPTRCWEMVRERVNQEITKQH 1049
Query: 88 RAGK 77
+AGK
Sbjct: 1050 KAGK 1053
>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 870
Score = 27.9 bits (59), Expect = 6.8
Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 2/56 (3%)
Frame = +1
Query: 403 DSSAE--LIDHRLEDIVNRMFQRCLEDGQYRQALGLSLETRRMDIFEESIMKSDDI 564
DSS E L RLE N + QR ++ + L SLE R+ + E + D+
Sbjct: 566 DSSGEDELAIQRLETTKNELRQRIAKEARGNAILQASLERRKQALHERRLSLEQDV 621
>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 822
Score = 27.9 bits (59), Expect = 6.8
Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 2/56 (3%)
Frame = +1
Query: 403 DSSAE--LIDHRLEDIVNRMFQRCLEDGQYRQALGLSLETRRMDIFEESIMKSDDI 564
DSS E L RLE N + QR ++ + L SLE R+ + E + D+
Sbjct: 566 DSSGEDELAIQRLETTKNELRQRIAKEARGNAILQASLERRKQALHERRLSLEQDV 621
>At4g39910.1 68417.m05653 ubiquitin-specific protease 3 (UBP3)
identical to GI:2347098
Length = 371
Score = 27.9 bits (59), Expect = 6.8
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = -1
Query: 597 CYCESVLKHSWYIIRFHNRLLE 532
CYC SVL+ ++ + F +LLE
Sbjct: 32 CYCNSVLQALYFCVPFREQLLE 53
>At1g65920.1 68414.m07480 regulator of chromosome condensation
(RCC1) family protein / zinc finger protein-related
contains Pfam profiles: regulator of chromosome
condensation (RCC1), PF01363 FYVE zinc finger
Length = 1006
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = -1
Query: 591 CESVLKHSWYIIRFHNRLLENIHTARLQ**AKRLS 487
C+S H W I F + + HT R+Q +R+S
Sbjct: 692 CDSCFDHLWSITEFSRNVKMDNHTPRMQMVTRRVS 726
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,306,611
Number of Sequences: 28952
Number of extensions: 285840
Number of successful extensions: 788
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 769
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 785
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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