Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0488
(460 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g07220.1 68414.m00768 expressed protein 40 6e-04
At1g76965.1 68414.m08961 glycine-rich protein 29 1.1
At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to ... 29 2.0
At5g42370.1 68418.m05159 expressed protein 29 2.0
At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identic... 29 2.0
At2g19150.1 68415.m02235 pectinesterase family protein contains ... 29 2.0
At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox... 28 2.6
At1g10390.1 68414.m01171 nucleoporin family protein contains Pfa... 28 3.5
At5g22390.1 68418.m02612 expressed protein 27 4.6
At1g10930.1 68414.m01255 DNA helicase (RECQl4A) nearly identical... 27 4.6
At3g56510.1 68416.m06284 TBP-binding protein, putative similar t... 27 6.1
At2g05210.1 68415.m00549 expressed protein 27 6.1
At2g03890.2 68415.m00352 phosphatidylinositol 3- and 4-kinase fa... 27 6.1
At2g03890.1 68415.m00351 phosphatidylinositol 3- and 4-kinase fa... 27 6.1
At5g56500.1 68418.m07051 chaperonin, putative similar to SWISS-P... 27 8.1
At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 27 8.1
At1g50410.1 68414.m05650 SNF2 domain-containing protein / helica... 27 8.1
>At1g07220.1 68414.m00768 expressed protein
Length = 507
Score = 40.3 bits (90), Expect = 6e-04
Identities = 27/88 (30%), Positives = 42/88 (47%), Gaps = 1/88 (1%)
Frame = -3
Query: 443 RIRFPSKPDTPRSSEPILIPKLRIQFADFP-YLHYSID*RLFTLETCCGYGYEPARHLHV 267
R+R P K +PRS +L+ L + F F L Y +D + +T G+ EP H+
Sbjct: 6 RLRLPHK-SSPRSPSYLLLCVLALSFFSFTALLFYKVDDFIAQTKTLAGHNLEPTPW-HI 63
Query: 266 HPSPEFSRSAESIRTPPQMRCSSRSEPY 183
P FS + + + ++CS S PY
Sbjct: 64 FPRKSFSAATKHSQAYRILQCSYFSCPY 91
>At1g76965.1 68414.m08961 glycine-rich protein
Length = 158
Score = 29.5 bits (63), Expect = 1.1
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = -3
Query: 455 PGTGRIRFPSKPDTPRSSEPILIPKLRIQFAD 360
PG + FP KP+ P P +P+L + F D
Sbjct: 93 PGAAIVVFPKKPEEPVKVVPTPMPQLNLFFGD 124
>At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to
autophagy 7 [Arabidopsis thaliana] GI:19912147; contains
Pfam profile PF00899: ThiF family
Length = 697
Score = 28.7 bits (61), Expect = 2.0
Identities = 13/45 (28%), Positives = 25/45 (55%)
Frame = -1
Query: 241 PQRVSGHRRKCGALRVPNHISLL*DSMELERSGRKENSSRTSRRR 107
P ++G CG +V NH++LL +S+ L+ ++S +R +
Sbjct: 42 PISITGFYGPCGHPQVSNHLTLLSESLPLDEQSLIASTSHGNRNK 86
>At5g42370.1 68418.m05159 expressed protein
Length = 447
Score = 28.7 bits (61), Expect = 2.0
Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = -2
Query: 180 PFYRIPWNSNAQAEKKTLPGPLGGVFRPL-WVTPSNTR 70
P Y + + Q+ +K +P PL + R L W TPS R
Sbjct: 285 PLYDVTSSGLVQSVEKVVPRPLRSIVRLLFWYTPSTMR 322
>At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identical
to low affinity calcium antiporter CAX2 (GI:1488267)
[Arabidopsis thaliana]; Ca2+:Cation Antiporter (CaCA)
Family member PMID:11500563
Length = 441
Score = 28.7 bits (61), Expect = 2.0
Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 2/46 (4%)
Frame = +1
Query: 43 SIIVPSSLKTSVRRGNPKWPEDAAERSGKSFLFC--LSVRVPWNPI 174
S++ SL TS + PK P+++ S K +FC L++ +P+ P+
Sbjct: 41 SLMEQGSLSTSFPQHTPKAPKNSVLNSIKIVIFCNKLNLLLPFGPL 86
>At2g19150.1 68415.m02235 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 339
Score = 28.7 bits (61), Expect = 2.0
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +1
Query: 211 ICGGVRILSADLENSGEGCTWRCRAGSYPYPQQV 312
I GG+ + ++ EGCT + R G YP P +V
Sbjct: 185 ISGGIDFIFGGAQSIFEGCTLKLRVGIYP-PNEV 217
>At1g72520.1 68414.m08386 lipoxygenase, putative similar to
lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140
[Lycopersicon esculentum], GB:CAB56692 [Arabidopsis
thaliana]
Length = 926
Score = 28.3 bits (60), Expect = 2.6
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = -3
Query: 236 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQK 138
+S + P R ++PYLPS G RTLR+K
Sbjct: 215 QSQKDHPSKRILFTNQPYLPSETPSGLRTLREK 247
>At1g10390.1 68414.m01171 nucleoporin family protein contains Pfam
profiles: PF04096 nucleoporin autopeptidase, PF03093
nucleoporin FG repeat family
Length = 1041
Score = 27.9 bits (59), Expect = 3.5
Identities = 28/105 (26%), Positives = 40/105 (38%), Gaps = 5/105 (4%)
Frame = -3
Query: 302 GYGYEPARHLHVHPSPEFSRSAESIRTPPQMRCSSRS-EPYLPSI--GFHGTRTLRQKRK 132
G+G P++ PSP F +++ + P S+ P P+I GT T
Sbjct: 410 GFGISPSQPNPFSPSPAFGQTSANPTNPFSSSTSTNPFAPQTPTIASSSFGTATSNFGSS 469
Query: 131 LFPDLSAASSGHFGLPRRTLVFKDEGTIIETVPLP--GSGIGTGF 3
F S+++ G T VF T P P GS GF
Sbjct: 470 PFGVTSSSNLFGSGSSTTTSVFGSSSAFGTTTPSPLFGSSSTPGF 514
>At5g22390.1 68418.m02612 expressed protein
Length = 202
Score = 27.5 bits (58), Expect = 4.6
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +1
Query: 115 ERSGKSFLFCLSVRVPWNPIEG 180
+ S KSFL LS PWNP +G
Sbjct: 17 DNSPKSFLDTLSSSSPWNPSKG 38
>At1g10930.1 68414.m01255 DNA helicase (RECQl4A) nearly identical to
DNA Helicase [Arabidopsis thaliana] GI:11121449
Length = 1188
Score = 27.5 bits (58), Expect = 4.6
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = -3
Query: 278 HLHVHPSPEFSRSAESIRTPPQMRCSSRSE 189
H H +P+ S S S+RTPP R +SR E
Sbjct: 229 HYHSTSTPQPSVSNFSLRTPPVDRSASRLE 258
>At3g56510.1 68416.m06284 TBP-binding protein, putative similar to
TBP-binding protein ABT1 GI:6518527 from [Mus musculus]
Length = 257
Score = 27.1 bits (57), Expect = 6.1
Identities = 17/68 (25%), Positives = 28/68 (41%)
Frame = -2
Query: 207 VLFAFRTISPFYRIPWNSNAQAEKKTLPGPLGGVFRPLWVTPSNTRF*RRGNDY*NGSAA 28
+L + + Y P +S AQ +K G G F WV + +R D NG
Sbjct: 69 ILAQYGELGRIYLAPEDSEAQVHRKRAGGFRGQRFSEGWVEFAKKSVAKRVADMLNGEQI 128
Query: 27 GFRNRNRI 4
G + ++ +
Sbjct: 129 GGKKKSSV 136
>At2g05210.1 68415.m00549 expressed protein
Length = 364
Score = 27.1 bits (57), Expect = 6.1
Identities = 18/52 (34%), Positives = 24/52 (46%)
Frame = -3
Query: 236 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQKRKLFPDLSAASSGHFGLPR 81
+S T C+ +E Y +GF T TLR+KR L S +F PR
Sbjct: 282 DSTATLEAFLCNKDAE-YFWGLGFQDTETLRKKRNWL--LGIRESSNFVAPR 330
>At2g03890.2 68415.m00352 phosphatidylinositol 3- and 4-kinase
family protein low similarity to phosphatidylinositol
4-kinase type-II beta [Homo sapiens] GI:20159767;
contains Pfam profile PF00454: Phosphatidylinositol 3-
and 4-kinase
Length = 530
Score = 27.1 bits (57), Expect = 6.1
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = -1
Query: 163 MELERSGRKENSSRTSRRRLQATLGYPVEHSFLKTRERLL 44
MEL+RS ++ T +RRLQ L +P E S L + +L
Sbjct: 60 MELDRS----DNVHTVKRRLQIALNFPTEESSLTYGDMVL 95
>At2g03890.1 68415.m00351 phosphatidylinositol 3- and 4-kinase
family protein low similarity to phosphatidylinositol
4-kinase type-II beta [Homo sapiens] GI:20159767;
contains Pfam profile PF00454: Phosphatidylinositol 3-
and 4-kinase
Length = 650
Score = 27.1 bits (57), Expect = 6.1
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = -1
Query: 163 MELERSGRKENSSRTSRRRLQATLGYPVEHSFLKTRERLL 44
MEL+RS ++ T +RRLQ L +P E S L + +L
Sbjct: 60 MELDRS----DNVHTVKRRLQIALNFPTEESSLTYGDMVL 95
>At5g56500.1 68418.m07051 chaperonin, putative similar to
SWISS-PROT:P08927- RuBisCO subunit binding-protein beta
subunit, chloroplast precursor (60 kDa chaperonin beta
subunit, CPN-60 beta) [Pisum sativum]; contains
Pfam:PF00118 domain, TCP-1/cpn60 chaperonin family
Length = 597
Score = 26.6 bits (56), Expect = 8.1
Identities = 17/50 (34%), Positives = 24/50 (48%)
Frame = -3
Query: 242 SAESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQKRKLFPDLSAASSGHF 93
S S PP R SS + S F T+++ Q++ FP + AA HF
Sbjct: 10 SMGSSLAPPSNRLSSFVS--ISSSSFGRTQSIAQRKARFPKIYAAKQLHF 57
>At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein similar to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 676
Score = 26.6 bits (56), Expect = 8.1
Identities = 10/22 (45%), Positives = 16/22 (72%)
Frame = -3
Query: 197 RSEPYLPSIGFHGTRTLRQKRK 132
R+ PY+P + F G RTL+ +R+
Sbjct: 288 RTFPYVPDMKFSGNRTLKLERQ 309
>At1g50410.1 68414.m05650 SNF2 domain-containing protein / helicase
domain-containing protein / RING finger
domain-containing protein similar to transcription
factor RUSH-1alpha [Oryctolagus cuniculus] GI:1655930;
contains Pfam profiles PF00271: Helicase conserved
C-terminal domain, PF00176: SNF2 family N-terminal
domain, PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 981
Score = 26.6 bits (56), Expect = 8.1
Identities = 14/43 (32%), Positives = 19/43 (44%)
Frame = -1
Query: 343 ILSTRGSSPWRPAADMGTNRRDISTYIPHLNFQGPQRVSGHRR 215
I TR P G++RRD+ST PH +GH +
Sbjct: 15 IQETRTRPQHPPRIAEGSHRRDLSTLRPHFLSGSSSGANGHTK 57
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,296,885
Number of Sequences: 28952
Number of extensions: 250740
Number of successful extensions: 785
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 759
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 785
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 762235320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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