Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0473
(642 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g08310.1 68417.m01372 expressed protein glutamic acid-rich pr... 33 0.16
At5g17930.1 68418.m02102 MA3 domain-containing protein low simil... 32 0.37
At4g19230.2 68417.m02837 cytochrome P450 family protein cytochro... 31 0.49
At4g02710.1 68417.m00366 kinase interacting family protein simil... 31 0.86
At3g19900.1 68416.m02520 expressed protein 29 2.0
At5g17240.1 68418.m02020 SET domain-containing protein contains ... 29 2.6
At5g31412.1 68418.m03722 hypothetical protein contains Pfam doma... 29 3.5
At1g66130.1 68414.m07505 oxidoreductase N-terminal domain-contai... 28 4.6
At5g64710.2 68418.m08135 expressed protein contains Pfam profile... 28 6.1
At5g64710.1 68418.m08134 expressed protein contains Pfam profile... 28 6.1
At3g04810.2 68416.m00521 protein kinase, putative similar to LST... 28 6.1
At3g04810.1 68416.m00520 protein kinase, putative similar to LST... 28 6.1
At1g57760.1 68414.m06553 expressed protein 28 6.1
At3g20860.1 68416.m02637 protein kinase family protein contains ... 27 8.0
At2g04230.1 68415.m00410 F-box family protein contains F-box dom... 27 8.0
At1g33670.1 68414.m04165 leucine-rich repeat family protein cont... 27 8.0
>At4g08310.1 68417.m01372 expressed protein glutamic acid-rich
protein precursor - Plasmodium falciparum, PIR2:A54514
Length = 504
Score = 33.1 bits (72), Expect = 0.16
Identities = 22/59 (37%), Positives = 30/59 (50%)
Frame = +1
Query: 85 DEQKENLKKHRADCLAETKADEQLVNKLKTGDFKTENEPLKKYALCMLIKSQLMTKDGK 261
D K +K+H CLA + DE N L+T + K + P+K+ A L K KDGK
Sbjct: 77 DVHKSFVKQHLVQCLAGAENDETSENSLET-EKKDDVTPVKEAA--ELSKEHTTKKDGK 132
>At5g17930.1 68418.m02102 MA3 domain-containing protein low
similarity to SP|Q9P6R9 Cell cycle control protein cwf22
{Schizosaccharomyces pombe}; contains Pfam profile
PF02847: MA3 domain
Length = 707
Score = 31.9 bits (69), Expect = 0.37
Identities = 26/104 (25%), Positives = 47/104 (45%), Gaps = 2/104 (1%)
Frame = +1
Query: 118 ADCLAETKADE--QLVNKLKTGDFKTENEPLKKYALCMLIKSQLMTKDGKFKKDVALAKV 291
A+ +AET E + LK + + N +K C+++ S+ F+K + L +
Sbjct: 479 AEDIAETMDAEVVEAQKMLKLAEAQRMNTDSRKAIFCVIMSSEDYID--AFEKLLRL-DL 535
Query: 292 PNAEDKLKVEKLIDACLANKGNSPHQTAWNYVKCYHEKDPKHAL 423
P +D+ + L++ CL K + T C H+K+ K L
Sbjct: 536 PGKQDREIMRVLVECCLQEKAFNKFYTVLASKLCEHDKNHKFTL 579
>At4g19230.2 68417.m02837 cytochrome P450 family protein cytochrome
P450, Arabidopsis thaliana; supported by cDNA:
gi_15293092_gb_AY050980.1_
Length = 484
Score = 31.5 bits (68), Expect = 0.49
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = -3
Query: 421 ERASGLSRGSISRNSRLFGEGCCLCWPGKRQS 326
ERA+G S G +SR C LCWPG R+S
Sbjct: 439 ERATGFSMG----HSRFPKTDCPLCWPGSRRS 466
>At4g02710.1 68417.m00366 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 1111
Score = 30.7 bits (66), Expect = 0.86
Identities = 18/52 (34%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = -3
Query: 502 LDKHVRASYLTLMRYGTVGL-DYVFTRKERASGLSRGSISRNSRLFGEGCCL 350
++K R+ + L+ L DYVFT +E A +S+G+ + L EG CL
Sbjct: 760 IEKLKRSQTIVLLNESIKSLEDYVFTHRESAGEVSKGADLMDEFLKLEGMCL 811
>At3g19900.1 68416.m02520 expressed protein
Length = 222
Score = 29.5 bits (63), Expect = 2.0
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = -1
Query: 171 LQFVHQLFISFRLGETVGSVFLQVLLLLICEGLSQDNADDEHDKSLHD 28
L F H L + G+T F ++ ++ EG + DN+DDE +SL D
Sbjct: 42 LAFSHSLCLKTTYGKTDRRKFSRICSIVDDEG-NLDNSDDEEKESLDD 88
>At5g17240.1 68418.m02020 SET domain-containing protein contains
Pfam profile PF00856: SET domain
Length = 491
Score = 29.1 bits (62), Expect = 2.6
Identities = 17/67 (25%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +1
Query: 202 LKKYALCMLI-KSQLMTKDGKFKKDVALAKVPNAEDKLKVEKLIDACLANKGNSPHQTAW 378
LKK L + + + LMT + KD+ L+ N + L +++ CL + + ++ W
Sbjct: 57 LKKGELVLKVPRKALMTTESIIAKDLKLSDAVNLHNSLSSTQILSVCLLYEMSKEKKSFW 116
Query: 379 NYVKCYH 399
Y +H
Sbjct: 117 -YPYLFH 122
>At5g31412.1 68418.m03722 hypothetical protein contains Pfam domain,
PF04937: Protein of unknown function (DUF 659)
Length = 433
Score = 28.7 bits (61), Expect = 3.5
Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 2/64 (3%)
Frame = +1
Query: 112 HRADCLAETKADEQLVNKLKT-GDFKTENEPLKKYALCMLIKSQLMTKD-GKFKKDVALA 285
H D + E + + K++T GD+K EN L +Y C+L + +D G K + LA
Sbjct: 227 HTVDLMLEAISKLPKMGKMQTFGDYKGENLFLNRYESCVLEWCDVCVEDFGPLMKLLRLA 286
Query: 286 KVPN 297
N
Sbjct: 287 DGEN 290
>At1g66130.1 68414.m07505 oxidoreductase N-terminal
domain-containing protein similar to AX110P [Daucus
carota] GI:285739; contains Pfam profile PF01408:
Oxidoreductase family NAD-binding Rossmann fold
Length = 364
Score = 28.3 bits (60), Expect = 4.6
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +1
Query: 265 KKDVALAKVPNAEDKLKVEKLIDACLANKGNSPHQTAW 378
KK L + P A+D ++EK+++AC N T W
Sbjct: 95 KKKHVLVEKPPAQDATELEKIVEACEYNGVQFMDGTIW 132
>At5g64710.2 68418.m08135 expressed protein contains Pfam profile
PF04396: Protein of unknown function, DUF537
Length = 716
Score = 27.9 bits (59), Expect = 6.1
Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 3/73 (4%)
Frame = +1
Query: 61 VVLAQALTDEQKENLKKHRADCLAETKADEQLVNKLKTGDFKTENEPLKKYALCMLI--- 231
VV + + K+N K + + ++ ++ V +K D K + EP+K L +
Sbjct: 237 VVSVEKMCQNMKQNDKDVKEESHQLQESSQEFVQVMKLMDVKAKEEPVKANQLAITAVDD 296
Query: 232 KSQLMTKDGKFKK 270
S KDG KK
Sbjct: 297 VSSFEEKDGFLKK 309
>At5g64710.1 68418.m08134 expressed protein contains Pfam profile
PF04396: Protein of unknown function, DUF537
Length = 841
Score = 27.9 bits (59), Expect = 6.1
Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 3/73 (4%)
Frame = +1
Query: 61 VVLAQALTDEQKENLKKHRADCLAETKADEQLVNKLKTGDFKTENEPLKKYALCMLI--- 231
VV + + K+N K + + ++ ++ V +K D K + EP+K L +
Sbjct: 362 VVSVEKMCQNMKQNDKDVKEESHQLQESSQEFVQVMKLMDVKAKEEPVKANQLAITAVDD 421
Query: 232 KSQLMTKDGKFKK 270
S KDG KK
Sbjct: 422 VSSFEEKDGFLKK 434
>At3g04810.2 68416.m00521 protein kinase, putative similar to
LSTK-1-like kinase [Lycopersicon esculentum]
GI:15637110; contains Pfam profile: PF00069 Eukaryotic
protein kinase domain
Length = 578
Score = 27.9 bits (59), Expect = 6.1
Identities = 23/85 (27%), Positives = 36/85 (42%), Gaps = 1/85 (1%)
Frame = +1
Query: 55 VCVVLAQALTDEQKENLKKHRADCLAETKADEQLVNKLKTGDFKTENEPLKKYALCMLIK 234
VC+++ + E +KK E K + LV L ++ N L + C I
Sbjct: 77 VCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYLHANHILHRDVKCSNI- 135
Query: 235 SQLMTKDGKFK-KDVALAKVPNAED 306
+TKD + D LAKV ++D
Sbjct: 136 --FLTKDQDIRLGDFGLAKVLTSDD 158
>At3g04810.1 68416.m00520 protein kinase, putative similar to
LSTK-1-like kinase [Lycopersicon esculentum]
GI:15637110; contains Pfam profile: PF00069 Eukaryotic
protein kinase domain
Length = 606
Score = 27.9 bits (59), Expect = 6.1
Identities = 23/85 (27%), Positives = 36/85 (42%), Gaps = 1/85 (1%)
Frame = +1
Query: 55 VCVVLAQALTDEQKENLKKHRADCLAETKADEQLVNKLKTGDFKTENEPLKKYALCMLIK 234
VC+++ + E +KK E K + LV L ++ N L + C I
Sbjct: 77 VCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYLHANHILHRDVKCSNI- 135
Query: 235 SQLMTKDGKFK-KDVALAKVPNAED 306
+TKD + D LAKV ++D
Sbjct: 136 --FLTKDQDIRLGDFGLAKVLTSDD 158
>At1g57760.1 68414.m06553 expressed protein
Length = 278
Score = 27.9 bits (59), Expect = 6.1
Identities = 23/75 (30%), Positives = 35/75 (46%)
Frame = -1
Query: 288 FSQSDVLLEFPVLGHQLRFDQHT*SVLLQWFVFSLKVACLQFVHQLFISFRLGETVGSVF 109
F + L F + LR D+ S LL + + + F + S RL E + S F
Sbjct: 131 FMVASSTLRFDLSPLLLRIDRVASSGLLIFAATKFTIKLILFHANIASSSRLLEILSS-F 189
Query: 108 LQVLLLLICEGLSQD 64
+ +LLL+I G+S D
Sbjct: 190 VLILLLMILSGISGD 204
>At3g20860.1 68416.m02637 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; contains
serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 427
Score = 27.5 bits (58), Expect = 8.0
Identities = 22/85 (25%), Positives = 37/85 (43%), Gaps = 1/85 (1%)
Frame = +1
Query: 55 VCVVLAQALTDEQKENLKKHRADCLAETKADEQLVNKLKTGDFKTENEPLKKYALCMLIK 234
VC+V + + + +KK R +E K +V L D+ N L + C I
Sbjct: 87 VCIVTSYCEGGDMTQMIKKSRGVFASEEKLCRWMVQLLLAIDYLHNNRVLHRDLKCSNI- 145
Query: 235 SQLMTKDGKFK-KDVALAKVPNAED 306
+TK+ + + D LAK+ +D
Sbjct: 146 --FLTKENEVRLGDFGLAKLLGKDD 168
>At2g04230.1 68415.m00410 F-box family protein contains F-box domain
Pfam:PF00646
Length = 448
Score = 27.5 bits (58), Expect = 8.0
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +1
Query: 295 NAEDKLKVEKLIDACLANKGNSPHQTAWNYVKCYHE 402
++ KL++ KL D L + +P + WN KC E
Sbjct: 333 DSSPKLQILKLTDVYLHDNKTNPDERKWNPPKCAPE 368
>At1g33670.1 68414.m04165 leucine-rich repeat family protein
contains leucine rich-repeat domains Pfam:PF00560,
INTERPRO:IPR001611; similar to receptor kinase-like
protein GB:AAB82755 GI:2586083 from [Oryza
longistaminata] (Science 270 (5243), 1804-1806 (1995))
Length = 455
Score = 27.5 bits (58), Expect = 8.0
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -1
Query: 297 IRHFSQSDVLLEFPVLGHQLRFD 229
IR F+Q D ++EF G++L+FD
Sbjct: 362 IRFFNQMDFMVEFHAPGNKLQFD 384
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,226,262
Number of Sequences: 28952
Number of extensions: 266518
Number of successful extensions: 719
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 696
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 718
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1324661040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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