Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0462
(692 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 343 2e-95
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 190 1e-49
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 188 5e-49
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 135 5e-33
SPBC2D10.06 |rep1|rec16|MBF transcription factor complex subunit... 28 1.5
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 27 3.4
SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 27 3.4
SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1 |... 27 3.4
SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC su... 26 4.5
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 26 5.9
SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit ... 26 5.9
SPAC140.03 |arb1||argonaute binding protein 1|Schizosaccharomyce... 26 5.9
SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyce... 25 7.9
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 343 bits (842), Expect = 2e-95
Identities = 155/212 (73%), Positives = 183/212 (86%)
Frame = +2
Query: 56 ISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDIC 235
+SKIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL I
Sbjct: 152 LSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIF 211
Query: 236 FRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFA 415
TLK+ +P+Y DLNHLVS M+GVTT RFPG+LN+DLRKLAVNMVPFPRLHFFM GFA
Sbjct: 212 ANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFA 271
Query: 416 PLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQML 595
PL + GS ++A++VPELTQQMFDA NMM A DPRHGRYLTVAA+FRG++SMKEVDEQ+
Sbjct: 272 PLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIR 331
Query: 596 NIQNKNSSYFVEWIPNNVKTGVCDIPPRGLKM 691
++Q KNS+YFVEWIP+NV VC +PP+ LKM
Sbjct: 332 SVQTKNSAYFVEWIPDNVLKAVCSVPPKDLKM 363
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 190 bits (464), Expect = 1e-49
Identities = 81/208 (38%), Positives = 130/208 (62%)
Frame = +2
Query: 56 ISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDIC 235
+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC
Sbjct: 158 LERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDIC 217
Query: 236 FRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFA 415
R L + PTY +LN L++ +S +T LRF G LN DL + N+VP+PR+HF + ++
Sbjct: 218 RRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYS 277
Query: 416 PLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQML 595
P+ S + + +V E+T Q F+ N M CDPR GRY+ ++RG + ++V +
Sbjct: 278 PIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVT 337
Query: 596 NIQNKNSSYFVEWIPNNVKTGVCDIPPR 679
+I+++ + FV+W P K G+C PP+
Sbjct: 338 SIKSRRTIQFVDWCPTGFKIGICYEPPQ 365
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 188 bits (459), Expect = 5e-49
Identities = 81/211 (38%), Positives = 129/211 (61%)
Frame = +2
Query: 56 ISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDIC 235
+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC
Sbjct: 154 LERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDIC 213
Query: 236 FRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFA 415
R L + P+Y +LN L++ +S +T LRF G LN DL + N+VP+PR+HF + +A
Sbjct: 214 RRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYA 273
Query: 416 PLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQML 595
P+ S + + +V E+T Q F+ N M CDPR GRY+ ++RG + ++V +
Sbjct: 274 PIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVT 333
Query: 596 NIQNKNSSYFVEWIPNNVKTGVCDIPPRGLK 688
I+ K + FV+W P K G+CD PP+ ++
Sbjct: 334 TIKAKRTIQFVDWCPTGFKIGICDRPPQHIE 364
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 135 bits (327), Expect = 5e-33
Identities = 67/212 (31%), Positives = 118/212 (55%), Gaps = 5/212 (2%)
Frame = +2
Query: 56 ISKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDI 232
+ ++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I
Sbjct: 154 LERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHI 213
Query: 233 CFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGF 412
L PT+ N LVS MS TT LR+PG +N DL + +++P PR HF + +
Sbjct: 214 AADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSY 273
Query: 413 APLTSRGSQQYRAL---TVPELTQQMFDAKNMMAACDP-RHGRYLTVAAIFRGRMSMKEV 580
P T++ ++ +A+ TV ++ +++ KN M + +P + ++++ I +G +V
Sbjct: 274 TPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADV 333
Query: 581 DEQMLNIQNKNSSYFVEWIPNNVKTGVCDIPP 676
+ +L I+ + + F+ W P +++ + P
Sbjct: 334 HKSLLRIRERRYASFIPWGPASIQVALSKKSP 365
>SPBC2D10.06 |rep1|rec16|MBF transcription factor complex subunit
Rep1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 472
Score = 27.9 bits (59), Expect = 1.5
Identities = 14/59 (23%), Positives = 27/59 (45%)
Frame = -3
Query: 471 VSSGTVSARYCWLPRDVSGAKPGMKKCRRGKGTMLTASLRRSAFSCPGNRRHVVTPDMV 295
++ G + R C + A+ K+ +GK T++ + + P RRH+ D+V
Sbjct: 350 IAYGGIPMRVCHSDSTLCDARIAAKQALKGKRQYDTSTSKAELSTPPSKRRHIDDADLV 408
>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 865
Score = 26.6 bits (56), Expect = 3.4
Identities = 22/68 (32%), Positives = 32/68 (47%)
Frame = +2
Query: 173 QLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADL 352
Q+ NTD + N LY RT+ DLN SL + GV +F G + +
Sbjct: 301 QVCTNTDSSRNAGNAILYQ-AVRTIL-------DLNSDSSLRVLGVNILAKFLGNRDNNT 352
Query: 353 RKLAVNMV 376
R +A+NM+
Sbjct: 353 RYVALNML 360
>SPBC56F2.07c |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 809
Score = 26.6 bits (56), Expect = 3.4
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = -3
Query: 360 SLRRSAFSCPGNRRHVVTPDMVSDTRWFRSPYVGVDSFSVRKQI 229
+LR NR ++ P ++ R R YVG +F RKQI
Sbjct: 679 ALRNVLVLAATNRPDMIDPALMRPGRLDRLLYVGPPNFEARKQI 722
>SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/53 (26%), Positives = 22/53 (41%)
Frame = -3
Query: 555 RKMAATVR*RPWRGSQAAIMFLASNICCVSSGTVSARYCWLPRDVSGAKPGMK 397
++MA+ R + A F A I CV + R W+ + G GM+
Sbjct: 496 KRMASLTNSREMQIQHAPTTFAAETIQCVLPIRIEKRVVWMEDGMEGGMEGMQ 548
>SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC
subunit Psc3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 962
Score = 26.2 bits (55), Expect = 4.5
Identities = 13/33 (39%), Positives = 22/33 (66%)
Frame = -3
Query: 633 HSTKYEEFLFWMLSICSSTSFMDMRPRKMAATV 535
+ST +E+ + W++++ SST MRP + ATV
Sbjct: 199 NSTVFEDIMSWVVAMSSST----MRPIRHTATV 227
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 25.8 bits (54), Expect = 5.9
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = -3
Query: 657 PVFTLLGIHSTKYEEFLFWMLSI 589
P+F L ++ T+Y ++L WM S+
Sbjct: 1859 PLFHFLDLNVTEYWDYLLWMDSV 1881
>SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit
Bgs4|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1955
Score = 25.8 bits (54), Expect = 5.9
Identities = 14/46 (30%), Positives = 22/46 (47%), Gaps = 1/46 (2%)
Frame = -3
Query: 528 RPWRGSQAAIMFLASNICCVSSGTVS-ARYCWLPRDVSGAKPGMKK 394
+P SQ A + S + C+ S Y ++PR G+KP K+
Sbjct: 550 KPTGASQWAAVACTSVVSCIIMAAASLCEYLFVPRRFPGSKPIWKR 595
>SPAC140.03 |arb1||argonaute binding protein 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 399
Score = 25.8 bits (54), Expect = 5.9
Identities = 13/44 (29%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = +2
Query: 527 RYLTVAAIFRGRMSMKEVDEQMLNIQNKN--SSYFVEWIPNNVK 652
RYLT + + +++K V + +LN N++ + F+ W P K
Sbjct: 297 RYLTGKVVEQEYLTVKLVSKTLLNFSNQSLCKAVFIVWDPPGSK 340
>SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 390
Score = 25.4 bits (53), Expect = 7.9
Identities = 11/39 (28%), Positives = 17/39 (43%)
Frame = +2
Query: 527 RYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPN 643
+Y+ ++F G + NI N S EW+PN
Sbjct: 29 QYIPTISVFEGSLIDNRDTLSYFNISNLEPSERSEWLPN 67
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,444,499
Number of Sequences: 5004
Number of extensions: 44419
Number of successful extensions: 187
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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