SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0459
         (533 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein L5|Sch...   136   3e-33
SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein L5|Schizo...   134   8e-33
SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pomb...    29   0.33 
SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|...    29   0.44 
SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces pomb...    29   0.58 
SPAC22F3.08c |rok1||ATP-dependent RNA helicase Rok1 |Schizosacch...    28   0.76 
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch...    27   1.3  
SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces po...    27   2.3  
SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces po...    26   3.1  
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos...    26   3.1  
SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual          26   3.1  
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa...    26   4.1  
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc...    26   4.1  
SPAC140.04 |||conserved fungal protein|Schizosaccharomyces pombe...    26   4.1  
SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual    25   5.4  
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|...    25   5.4  
SPBC713.07c |||vacuolar polyphosphatase |Schizosaccharomyces pom...    25   7.1  
SPAC18G6.02c |chp1||chromodomain protein Chp1|Schizosaccharomyce...    25   7.1  
SPCC126.05c |mrpl17||mitochondrial ribosomal protein subunit L17...    25   9.4  
SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosacchar...    25   9.4  
SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyc...    25   9.4  
SPBC31E1.06 |bms1|SPBC800.01|GTP binding protein Bms1|Schizosacc...    25   9.4  
SPAC1B9.03c ||SPAC6B12.01|RNA-binding protein|Schizosaccharomyce...    25   9.4  
SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces p...    25   9.4  

>SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein
           L5|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 294

 Score =  136 bits (328), Expect = 3e-33
 Identities = 67/138 (48%), Positives = 92/138 (66%), Gaps = 2/138 (1%)
 Frame = +3

Query: 30  LTRTTTGARVFGAMKGAVDGGLNVPHSIKRFPGYDAESKKFNAEVHRAHIFGLHVAEYMR 209
           L RT+TG+RVFGAMKGA DGGL +PHS  RFPG+D E+++ + E  R +I+G HVAEYM 
Sbjct: 150 LKRTSTGSRVFGAMKGASDGGLFIPHSPNRFPGFDIETEELDDETLRKYIYGGHVAEYME 209

Query: 210 SLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKK--KELKKDSVKQKR 383
            L  DDE+ +++QFS  I  G+ +D +E IY +A+  IR DPS +K  K+      +  +
Sbjct: 210 MLIDDDEERYQKQFSGLIADGIESDQLEDIYAEAYAKIREDPSFQKSGKDAAAFKAESLK 269

Query: 384 WNKRKLTLAERKNRIKQK 437
           + +RKLT  ERK R   K
Sbjct: 270 YTQRKLTAEERKERFNAK 287


>SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein
           L5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 294

 Score =  134 bits (324), Expect = 8e-33
 Identities = 67/138 (48%), Positives = 91/138 (65%), Gaps = 2/138 (1%)
 Frame = +3

Query: 30  LTRTTTGARVFGAMKGAVDGGLNVPHSIKRFPGYDAESKKFNAEVHRAHIFGLHVAEYMR 209
           L RT+TG+RVFGAMKGA DGGL +PHS  RFPG+D E+++ + E  R +I+G HVAEYM 
Sbjct: 150 LKRTSTGSRVFGAMKGASDGGLFIPHSPNRFPGFDIETEELDDETLRKYIYGGHVAEYME 209

Query: 210 SLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKK--KELKKDSVKQKR 383
            L  DDE+ +++QFS  I  G+ +D +E IY +A+  IR DPS +K  K+      +  +
Sbjct: 210 MLIDDDEERYQKQFSGLIADGIESDQLEDIYAEAYAKIREDPSFQKSGKDAAAFKAESLK 269

Query: 384 WNKRKLTLAERKNRIKQK 437
             +RKLT  ERK R   K
Sbjct: 270 HTQRKLTAEERKERFNAK 287


>SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 508

 Score = 29.5 bits (63), Expect = 0.33
 Identities = 19/53 (35%), Positives = 27/53 (50%)
 Frame = +3

Query: 288 IEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKAS 446
           IE   KK+ +   AD    KKE K+   K+ + +KRK   +E     K+KK S
Sbjct: 451 IEKKKKKSSKLKEADGESSKKEKKEKKDKKHKKSKRKSEESEDGESPKKKKKS 503


>SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1184

 Score = 29.1 bits (62), Expect = 0.44
 Identities = 17/59 (28%), Positives = 35/59 (59%), Gaps = 2/59 (3%)
 Frame = +3

Query: 303 KKAHEAIRADPSHKKKELKKDSVKQKRWNKR--KLTLAERKNRIKQKKASFIKRLQAQA 473
           + A E  R + + + K LKK + ++KR  K+  K   AE K R+++++A + K++  ++
Sbjct: 708 RAAQERERKEQAKEAKRLKKLAKEEKRLKKKEEKARKAEEK-RLQKERAKYAKQMSRES 765


>SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 497

 Score = 28.7 bits (61), Expect = 0.58
 Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
 Frame = +3

Query: 315 EAIRADPSHKKKELKKDSVKQKRWNKRKL-TLAERKNRIKQKKAS 446
           E I   PS K+K+ KK+  K+K   KR     +E   + K+KK S
Sbjct: 452 ETISEKPSKKEKKDKKEKKKEKSKKKRSADDASEEVKKSKKKKKS 496


>SPAC22F3.08c |rok1||ATP-dependent RNA helicase Rok1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 481

 Score = 28.3 bits (60), Expect = 0.76
 Identities = 22/79 (27%), Positives = 38/79 (48%)
 Frame = +3

Query: 237 FKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAER 416
           F ++  +YIKL   A  + +   +    + A P    KE+KK   K     KR  T A  
Sbjct: 392 FTKEDGEYIKL--IAGVMRSSGCEVPNWVMALPK-PSKEMKKKLKKSPPKRKRITTRASY 448

Query: 417 KNRIKQKKASFIKRLQAQA 473
             + +Q+K  +IK+++ +A
Sbjct: 449 DRQKEQRKKEYIKKVKKEA 467


>SPCC162.08c |nup211||nuclear pore complex associated
            protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1837

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 13/54 (24%), Positives = 29/54 (53%)
 Frame = +3

Query: 219  QDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKKKELKKDSVKQK 380
            +++++S  ++ +K ++  V        +KK +E IR+D       LK+D  K++
Sbjct: 1023 KEEKESSSKELAKQLEDAVREKDSALSFKKDYEKIRSDADRVITSLKEDIEKER 1076


>SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 474

 Score = 26.6 bits (56), Expect = 2.3
 Identities = 13/38 (34%), Positives = 22/38 (57%)
 Frame = +3

Query: 285 AIEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRK 398
           ++E+  K   EA + +   +KKE KK+  K+K+  K K
Sbjct: 430 SVESSEKDEDEAAKKEEKRRKKEAKKEK-KEKKEKKEK 466


>SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 284

 Score = 26.2 bits (55), Expect = 3.1
 Identities = 22/79 (27%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
 Frame = +3

Query: 216 EQDDEDSFKRQFSKYIKLGVTADA--IEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWN 389
           E+  + S K++  K    G   D   +E I KK  +       HKKK+ +  S K+K  +
Sbjct: 166 EKRKKHSSKKKSKKKTSTGSALDPKKLEKITKKKKK------EHKKKDKESSSKKRKSGS 219

Query: 390 KRKLTLAERKNRIKQKKAS 446
             K    ++K ++K K  S
Sbjct: 220 SDKEEKKKKKIKLKDKPES 238


>SPBC17D11.05 |tif32||translation initiation factor
           eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 932

 Score = 26.2 bits (55), Expect = 3.1
 Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
 Frame = +3

Query: 291 EAIYKKAHEAIRADPSHKKKELKKDSVKQKR-WNKRKLTLAERKNRIKQKKASFIKR 458
           EAI +   EA RA      +EL +    QKR   +R     E K R +++ A  ++R
Sbjct: 788 EAIREAEEEAARAAEEEANRELHEQEEAQKRAIEERTRAAREAKEREQREMAEKLER 844


>SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 674

 Score = 26.2 bits (55), Expect = 3.1
 Identities = 15/28 (53%), Positives = 19/28 (67%)
 Frame = +3

Query: 276 TADAIEAIYKKAHEAIRADPSHKKKELK 359
           TA  IEA+YKKA+  I+ D +H  K LK
Sbjct: 174 TAQLIEAVYKKAN--IK-DAAHDSKNLK 198


>SPAC19A8.08 |upf2||nonsense-mediated decay protein
           Upf2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1049

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 23/101 (22%), Positives = 39/101 (38%)
 Frame = +3

Query: 150 FNAEVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRA 329
           FNA   R+    +  A+       D E  F  +       G  A+   +IY+ + E IR+
Sbjct: 363 FNASGERSESANVETAQVW----DDREQYFFYEVFPNFNEGSIAEMKSSIYESSQEGIRS 418

Query: 330 DPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKASFI 452
              + KKE   D +K    +     ++ R +    K  S +
Sbjct: 419 SSENNKKE---DDLKDSTGDLNTTQVSSRVDNFLLKLPSMV 456


>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1085

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 17/60 (28%), Positives = 33/60 (55%)
 Frame = +3

Query: 291 EAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKASFIKRLQAQ 470
           EA  K+  + ++ +   K++EL++    QKR  K+K    E+K + +Q++A   K  + Q
Sbjct: 636 EARKKREEQRLKREQEKKQQELER----QKREEKQKQKEREKKLKKQQQEADREKMAREQ 691



 Score = 24.6 bits (51), Expect = 9.4
 Identities = 19/57 (33%), Positives = 33/57 (57%), Gaps = 2/57 (3%)
 Frame = +3

Query: 306 KAHEAIRADPSHKK-KELKKDSVKQKRWNKRK-LTLAERKNRIKQKKASFIKRLQAQ 470
           K  E I  +   K+ +ELKK   K+K+ +K+K L LA+ + R ++++A  +    AQ
Sbjct: 571 KLLEEIEEENKRKQERELKKIREKEKKRDKKKQLKLAKEEER-QRREAERLAEQAAQ 626


>SPAC140.04 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 295

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 14/52 (26%), Positives = 26/52 (50%)
 Frame = +3

Query: 303 KKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKASFIKR 458
           KK +E  +   SH+K   K          KRKL + +++   K+ +++ IK+
Sbjct: 27  KKRYEDEKRKISHQKYTDKLLEANDNETTKRKLLVKQKRKSNKEFQSNIIKK 78


>SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 978

 Score = 25.4 bits (53), Expect = 5.4
 Identities = 10/24 (41%), Positives = 17/24 (70%)
 Frame = +3

Query: 195  AEYMRSLEQDDEDSFKRQFSKYIK 266
            +EY+++L   D  SFK+ F K+I+
Sbjct: 948  SEYLQALSNLDSRSFKQFFQKFIQ 971


>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 207

 Score = 25.4 bits (53), Expect = 5.4
 Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 2/49 (4%)
 Frame = +3

Query: 303 KKAHEAIRADPSHKKKE--LKKDSVKQKRWNKRKLTLAERKNRIKQKKA 443
           KK  E   A  S K+ E   KKD+ + ++W+K   T  + +   K+K A
Sbjct: 5   KKRAEKAEAAKSRKQDEEKKKKDAEEDEKWSKGVKTNKKEQEAEKRKAA 53


>SPBC713.07c |||vacuolar polyphosphatase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 577

 Score = 25.0 bits (52), Expect = 7.1
 Identities = 11/19 (57%), Positives = 15/19 (78%)
 Frame = +3

Query: 342 KKKELKKDSVKQKRWNKRK 398
           KKK+ KK + K+K+ NKRK
Sbjct: 436 KKKKKKKKNNKKKKKNKRK 454


>SPAC18G6.02c |chp1||chromodomain protein Chp1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 960

 Score = 25.0 bits (52), Expect = 7.1
 Identities = 12/36 (33%), Positives = 22/36 (61%)
 Frame = +3

Query: 330 DPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQK 437
           D   KK + +K+ ++Q+R  KRK  L +   ++K+K
Sbjct: 89  DNEAKKMKREKEILRQQR-QKRKSELTQLSQKVKEK 123


>SPCC126.05c |mrpl17||mitochondrial ribosomal protein subunit
           L17|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 268

 Score = 24.6 bits (51), Expect = 9.4
 Identities = 11/32 (34%), Positives = 19/32 (59%)
 Frame = +3

Query: 327 ADPSHKKKELKKDSVKQKRWNKRKLTLAERKN 422
           +DP   +   KK SV +K W +R+ TL  +++
Sbjct: 76  SDPLPAEFYFKKGSVGEKDWQERQKTLKGKES 107


>SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 598

 Score = 24.6 bits (51), Expect = 9.4
 Identities = 11/44 (25%), Positives = 22/44 (50%)
 Frame = +3

Query: 291 EAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKN 422
           E I +        +  H++K+ ++  V++K   KRK    +R+N
Sbjct: 510 ETITRSGIAISSTEMDHQQKQARRRRVRRKHAEKRKQMAEKRRN 553


>SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 682

 Score = 24.6 bits (51), Expect = 9.4
 Identities = 16/46 (34%), Positives = 27/46 (58%), Gaps = 2/46 (4%)
 Frame = +3

Query: 348 KELKKDSVKQKRWNKRKLTLAER--KNRIKQKKASFIKRLQAQAEA 479
           +E + DS +   ++ + +  AE+  KNR  +KKAS ++ LQ   EA
Sbjct: 553 REDENDSEQLDHFDMKSILKAEKFKKNRKLKKKASNLEGLQEGFEA 598


>SPBC31E1.06 |bms1|SPBC800.01|GTP binding protein
           Bms1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1121

 Score = 24.6 bits (51), Expect = 9.4
 Identities = 14/44 (31%), Positives = 20/44 (45%)
 Frame = +3

Query: 303 KKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQ 434
           KK H A  + P  +KK+LKK S      N +   +A      +Q
Sbjct: 4   KKGHYAKHSGPKAEKKKLKKVSDGSASNNPKAFAVASAGRMARQ 47


>SPAC1B9.03c ||SPAC6B12.01|RNA-binding protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 389

 Score = 24.6 bits (51), Expect = 9.4
 Identities = 12/29 (41%), Positives = 20/29 (68%)
 Frame = +3

Query: 312 HEAIRADPSHKKKELKKDSVKQKRWNKRK 398
           HE  RA    +KKE + ++V++KR NK++
Sbjct: 332 HEQSRALKEKRKKE-QDENVRRKRENKKR 359


>SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 525

 Score = 24.6 bits (51), Expect = 9.4
 Identities = 13/41 (31%), Positives = 19/41 (46%)
 Frame = +3

Query: 168 RAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAI 290
           R  + GL VAEY R +E  D   F     ++ + G    A+
Sbjct: 277 RVALTGLTVAEYFRDIEGQDVLLFIDNIFRFTQAGSEVSAL 317


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,961,746
Number of Sequences: 5004
Number of extensions: 36665
Number of successful extensions: 203
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 220420454
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -