BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0454 (409 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schiz... 27 1.5 SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 26 2.6 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 2.6 >SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schizosaccharomyces pombe|chr 1|||Manual Length = 504 Score = 26.6 bits (56), Expect = 1.5 Identities = 10/21 (47%), Positives = 15/21 (71%) Frame = -1 Query: 130 VFFEINFVNYSKFKNIMNLYD 68 VF E+N + K KN++NL+D Sbjct: 109 VFKEVNIMRRVKHKNVVNLFD 129 >SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1480 Score = 25.8 bits (54), Expect = 2.6 Identities = 9/29 (31%), Positives = 18/29 (62%) Frame = -1 Query: 121 EINFVNYSKFKNIMNLYDKKCIYKKKTDE 35 E+N +NYS ++N++ K+ Y + D+ Sbjct: 814 EVNLLNYSLENVVLNIFKKQTPYYNQADK 842 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 2.6 Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 3/45 (6%) Frame = +3 Query: 252 YKNRIGALTSLIRCES--GVGQKKKKT-GFPFKPLLSLKT*LPYH 377 YK +G T++I CE G+G +KT F K + S +T +H Sbjct: 1050 YKEGLGNTTTIICCEPVIGLGHNYQKTYAFKRKDVTSFRTLDDFH 1094 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,656,570 Number of Sequences: 5004 Number of extensions: 31731 Number of successful extensions: 58 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 58 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 58 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 140222766 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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