Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0453
(681 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g39850.1 68418.m04829 40S ribosomal protein S9 (RPS9C) 40S ri... 208 3e-54
At5g15200.1 68418.m01781 40S ribosomal protein S9 (RPS9B) 40S ri... 203 7e-53
At5g15750.1 68418.m01842 RNA-binding S4 domain-containing protei... 36 0.033
At5g01850.1 68418.m00104 protein kinase, putative similar to pro... 29 3.8
At1g24490.1 68414.m03084 60 kDa inner membrane family protein si... 29 3.8
At5g67320.1 68418.m08490 WD-40 repeat family protein strong simi... 28 5.0
At3g45880.1 68416.m04965 hypothetical protein 28 5.0
At1g43730.1 68414.m05028 hypothetical protein 28 5.0
At5g41240.1 68418.m05011 glutathione S-transferase, putative sim... 27 8.7
At5g28615.1 68418.m03493 hypothetical protein 27 8.7
At4g32190.1 68417.m04581 centromeric protein-related low similar... 27 8.7
At4g13620.1 68417.m02120 AP2 domain-containing transcription fac... 27 8.7
>At5g39850.1 68418.m04829 40S ribosomal protein S9 (RPS9C) 40S
ribosomal protein S9 - Chlamydomonas sp.,EMBL:AU066528
Length = 197
Score = 208 bits (507), Expect = 3e-54
Identities = 98/137 (71%), Positives = 117/137 (85%)
Frame = +3
Query: 15 MVNNRLPSVFSKTYVTPRRPFEKARLDQELKIIGEYGLRNKREVWRVKYTLARIRKAARE 194
MVN R + KT+ PRRP+EK RLD ELK++GEYGLR KRE+WRV+YTL+RIR AARE
Sbjct: 1 MVNVRFYRNYGKTFKKPRRPYEKERLDAELKLVGEYGLRCKRELWRVQYTLSRIRNAARE 60
Query: 195 LLTLEEKDPKRLFEGNALLRRLVRIGVLDEKQMKLDYVLGLKIEDFLERRLQTQVFKAGL 374
LLTL+EK+P+R+FEG ALLRR+ R G+LDE Q KLDYVL L +E+FLERRLQT VFK+G+
Sbjct: 61 LLTLDEKNPRRIFEGEALLRRMNRYGLLDETQNKLDYVLALTVENFLERRLQTIVFKSGM 120
Query: 375 AKSIHHARILIRQRHIR 425
AKSIHHAR+LIRQRHIR
Sbjct: 121 AKSIHHARVLIRQRHIR 137
Score = 51.6 bits (118), Expect = 5e-07
Identities = 21/29 (72%), Positives = 27/29 (93%)
Frame = +2
Query: 572 KQVVNIPSFIVRLDSGKHIDFSLKSPFGG 658
+Q+VNIPSF+VR++S KH+DFSL SPFGG
Sbjct: 140 RQLVNIPSFMVRVESQKHVDFSLTSPFGG 168
>At5g15200.1 68418.m01781 40S ribosomal protein S9 (RPS9B) 40S
ribosomal protein S9, Chlamydomonas sp., EMBL:AU066528
Length = 198
Score = 203 bits (496), Expect = 7e-53
Identities = 92/128 (71%), Positives = 113/128 (88%)
Frame = +3
Query: 42 FSKTYVTPRRPFEKARLDQELKIIGEYGLRNKREVWRVKYTLARIRKAARELLTLEEKDP 221
+ KT+ PRRP+EK RLD ELK++GEYGLRNKRE+WRV+Y+L+RIR AAR+LLTL+EK P
Sbjct: 10 YGKTFKGPRRPYEKERLDSELKLVGEYGLRNKRELWRVQYSLSRIRNAARDLLTLDEKSP 69
Query: 222 KRLFEGNALLRRLVRIGVLDEKQMKLDYVLGLKIEDFLERRLQTQVFKAGLAKSIHHARI 401
+R+FEG ALLRR+ R G+LDE Q KLDYVL L +E+FLERRLQT VFK+G+AKSIHH+R+
Sbjct: 70 RRIFEGEALLRRMNRYGLLDESQNKLDYVLALTVENFLERRLQTIVFKSGMAKSIHHSRV 129
Query: 402 LIRQRHIR 425
LIRQRHIR
Sbjct: 130 LIRQRHIR 137
Score = 54.8 bits (126), Expect = 5e-08
Identities = 24/29 (82%), Positives = 27/29 (93%)
Frame = +2
Query: 572 KQVVNIPSFIVRLDSGKHIDFSLKSPFGG 658
KQ+VNIPSF+VRLDS KHIDF+L SPFGG
Sbjct: 140 KQLVNIPSFMVRLDSQKHIDFALTSPFGG 168
>At5g15750.1 68418.m01842 RNA-binding S4 domain-containing protein
40S RIBOSOMAL PROTEINs - different species
Length = 182
Score = 35.5 bits (78), Expect = 0.033
Identities = 25/110 (22%), Positives = 48/110 (43%)
Frame = +3
Query: 96 QELKIIGEYGLRNKREVWRVKYTLARIRKAARELLTLEEKDPKRLFEGNALLRRLVRIGV 275
+E +I Y + ++ + + ++K + ++ DP R+ + LL +L +GV
Sbjct: 27 RENEITYRYHMGSRDDYKKYSGLCRMVQKLTNIMKQMDPADPFRIQMTDMLLEKLYNMGV 86
Query: 276 LDEKQMKLDYVLGLKIEDFLERRLQTQVFKAGLAKSIHHARILIRQRHIR 425
+ ++ L L + F RRL T + A+ A I Q H+R
Sbjct: 87 IPTRK-SLTLTERLSVSSFCRRRLSTVLVHLKFAEHHKEAVTYIEQGHVR 135
>At5g01850.1 68418.m00104 protein kinase, putative similar to
protein kinase [Arabidopsis thaliana]
gi|1054633|emb|CAA63387; contains protein kinase domain,
Pfam:PF00069
Length = 333
Score = 28.7 bits (61), Expect = 3.8
Identities = 12/18 (66%), Positives = 15/18 (83%)
Frame = -3
Query: 652 EWRFQREVNVLARVQAHN 599
E RF REVN+++RVQ HN
Sbjct: 60 ESRFVREVNMMSRVQHHN 77
>At1g24490.1 68414.m03084 60 kDa inner membrane family protein
similar to chloroplast membrane protein (ALBINO3)
(GI:3927828) [Arabidopsis thaliana]
Length = 1013
Score = 28.7 bits (61), Expect = 3.8
Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 2/41 (4%)
Frame = +3
Query: 48 KTYVTPRR-PFEKARLDQ-ELKIIGEYGLRNKREVWRVKYT 164
K ++ PRR P + Q E K EYG RN E W + T
Sbjct: 479 KYFIKPRRHPESECSATQTEYKFTSEYGKRNSSECWAMTTT 519
>At5g67320.1 68418.m08490 WD-40 repeat family protein strong
similarity to unknown protein (ref|NP_005638.1)
Length = 613
Score = 28.3 bits (60), Expect = 5.0
Identities = 12/28 (42%), Positives = 14/28 (50%)
Frame = +2
Query: 287 TDETRLCAWSED*GLLGASSADAGVQSW 370
T E CAWS LL + S DA + W
Sbjct: 265 TSEVCACAWSPSASLLASGSGDATARIW 292
>At3g45880.1 68416.m04965 hypothetical protein
Length = 431
Score = 28.3 bits (60), Expect = 5.0
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -3
Query: 589 DVHNLLADNYYQTNASYYENTNCFK 515
DVH L + Y N SY+ +T+ FK
Sbjct: 198 DVHRLYIEQYPAANYSYHRDTDAFK 222
>At1g43730.1 68414.m05028 hypothetical protein
Length = 320
Score = 28.3 bits (60), Expect = 5.0
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = -1
Query: 405 SKFWHDGWTSPGQL 364
+KFWHD WT G L
Sbjct: 77 AKFWHDNWTGHGPL 90
>At5g41240.1 68418.m05011 glutathione S-transferase, putative
similar to glutathione S-transferase, GST 10b
GB:CAA10662 [Arabidopsis thaliana] 37349.
Length = 591
Score = 27.5 bits (58), Expect = 8.7
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +3
Query: 255 RLVRIGVLDEKQMKLDYVLGLKIEDFLERRLQTQ 356
RL + VLDE++ +D LG+K ER++ T+
Sbjct: 473 RLENLWVLDEEEQVMDLPLGVKSSKQKERKVATK 506
>At5g28615.1 68418.m03493 hypothetical protein
Length = 149
Score = 27.5 bits (58), Expect = 8.7
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = -1
Query: 405 SKFWHDGWTSPGQL 364
+KFWHD WT G L
Sbjct: 9 AKFWHDDWTGLGPL 22
>At4g32190.1 68417.m04581 centromeric protein-related low similarity
to SP|Q02224 Centromeric protein E (CENP-E protein)
{Homo sapiens}
Length = 783
Score = 27.5 bits (58), Expect = 8.7
Identities = 14/48 (29%), Positives = 25/48 (52%)
Frame = +3
Query: 117 EYGLRNKREVWRVKYTLARIRKAARELLTLEEKDPKRLFEGNALLRRL 260
+YG+ NKR V + +T +R E+L ++ + E N ++ RL
Sbjct: 599 DYGMENKRLVMELSFTRENLRMKEMEVLAVQRALTFKDEEINVVMGRL 646
>At4g13620.1 68417.m02120 AP2 domain-containing transcription
factor, putative similar to AP2 domain containing
protein RAP2.4 [Arabidopsis thaliana] GI:2281633;
contains Pfam profile PF00847: AP2 domain
Length = 388
Score = 27.5 bits (58), Expect = 8.7
Identities = 14/56 (25%), Positives = 27/56 (48%)
Frame = -3
Query: 670 PGRPPTEWRFQREVNVLARVQAHNK*WDVHNLLADNYYQTNASYYENTNCFKTLNS 503
P +PP F +E +L Q+ + H + +++YQ++ + E KTL +
Sbjct: 141 PSKPPNLTLFLKEPKLLELSQSESNMSPYHKYIPNSFYQSDQNRNEWVEINKTLTN 196
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,430,222
Number of Sequences: 28952
Number of extensions: 287225
Number of successful extensions: 793
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 777
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 792
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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