Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0452
         (702 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1105.18c ||SPBC887.21c|peptide release factor|Schizosaccharo...    75   7e-15
SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces p...    49   7e-07
SPBC13G1.06c |isd11||iron sulfur cluster assembly protein Isd11|...    36   0.006
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po...    33   0.030
SPAC589.11 |mug82||translation release factor |Schizosaccharomyc...    28   1.5  
SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces...    25   7.9  
SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogam...    25   7.9  
SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces pomb...    25   7.9  
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy...    25   7.9  
SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyc...    25   7.9  

>SPBC1105.18c ||SPBC887.21c|peptide release
           factor|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 162

 Score = 75.4 bits (177), Expect = 7e-15
 Identities = 33/106 (31%), Positives = 67/106 (63%), Gaps = 2/106 (1%)
 Frame = +1

Query: 382 KVPKINECELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHTSRCQDDNRKKARE 561
           ++ ++ E ++ E F+ G GPGG  +NK S    + HIPTG +++   +R ++ NR  AR+
Sbjct: 43  QLERLQEEDIEETFICGKGPGGQKINKTSIVAQVKHIPTGIIVRSQDTRSREQNRCIARK 102

Query: 562 LLIEKLDDMINGPESVSAHR--KLIEEKKYKRNATKKQKISKLKDE 693
            L EK+D+  +G +S+ A +  +++++KK +   +K++  +K+ D+
Sbjct: 103 RLTEKVDEFKHGNDSLLARKVQRIVKKKKQREKKSKRKYGNKIDDQ 148


>SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 396

 Score = 48.8 bits (111), Expect = 7e-07
 Identities = 28/90 (31%), Positives = 47/90 (52%)
 Frame = +1

Query: 406 ELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHTSRCQDDNRKKARELLIEKLDD 585
           E+  + +R  G GG  VN+  + V LTHIPTG  +    SR Q  N++KA  +L  +L  
Sbjct: 256 EVKIEVMRSRGAGGQHVNRTESAVRLTHIPTGITVSMQDSRSQHQNKEKAFLVLNSRL-A 314

Query: 586 MINGPESVSAHRKLIEEKKYKRNATKKQKI 675
            +N  +   A R  ++ K    ++ + +K+
Sbjct: 315 ALNAAKENEAER--LKRKNQVTSSDRSEKL 342


>SPBC13G1.06c |isd11||iron sulfur cluster assembly protein
           Isd11|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 102

 Score = 35.9 bits (79), Expect = 0.006
 Identities = 22/70 (31%), Positives = 38/70 (54%), Gaps = 3/70 (4%)
 Frame = +3

Query: 96  MNITRKEVLRLYRNLLMYSKSLKLTDPDYFRRSIKIEFKNNQNLTDLEKISFAYQKGQAL 275
           M+++++ V+RLYRN+L  SK    T  +Y  R  + +FK  +  +D  K     +  + L
Sbjct: 1   MSVSKQHVVRLYRNILKTSKLFPYTYREYTIRRTRDKFKELKVESDPAKFEQGIKDSEKL 60

Query: 276 L---QRGSVV 296
           L   QR S++
Sbjct: 61  LEIIQRQSII 70


>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 646

 Score = 33.5 bits (73), Expect = 0.030
 Identities = 18/53 (33%), Positives = 32/53 (60%), Gaps = 1/53 (1%)
 Frame = +1

Query: 547 KKARELLIEK-LDDMINGPESVSAHRKLIEEKKYKRNATKKQKISKLKDEWKK 702
           K+  E++++  +D+ +   ES+SA RK +  K Y  + T+KQK+   + E KK
Sbjct: 575 KQLYEVILQAVIDNRVVARESISALRKNVTAKCYGGDVTRKQKLLNKQKEGKK 627


>SPAC589.11 |mug82||translation release factor |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 182

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 14/32 (43%), Positives = 18/32 (56%)
 Frame = +1

Query: 421 FVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKC 516
           F R SGPGG  VNK  N  V+ ++P   +  C
Sbjct: 53  FSRSSGPGGQNVNK-LNTKVIVNLPFKQLESC 83


>SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 324

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 14/52 (26%), Positives = 27/52 (51%)
 Frame = +1

Query: 532 QDDNRKKARELLIEKLDDMINGPESVSAHRKLIEEKKYKRNATKKQKISKLK 687
           Q    KK  ELL ++ ++       ++  RK ++E   K+    +QKIS+++
Sbjct: 18  QKRKSKKMEELLSKQREECKELQSKITNLRKQLKEGNKKQKRALQQKISQME 69


>SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogamy
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 543

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 14/53 (26%), Positives = 23/53 (43%)
 Frame = +1

Query: 136 IC*CTRNH*NSPTQIIFGAQLKSNSKIIKT*LIWRKLASRIRKVRPCCREGQL 294
           +C   R+   +P++ + G+Q +  SK+  T   W    S    V   CR   L
Sbjct: 64  LCDFERSQILAPSECVRGSQSECVSKLESTSTWWLSFTSHFHDVNHLCRLANL 116


>SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 840

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 11/32 (34%), Positives = 22/32 (68%), Gaps = 1/32 (3%)
 Frame = +3

Query: 147 YSKSLKLTDPDYFRRSIKIEFKN-NQNLTDLE 239
           YS+++ L + + F R I IEF++ + +L D++
Sbjct: 408 YSRTVTLDEMEQFTREISIEFRSISSSLRDVD 439


>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 321

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +1

Query: 361 KHTIDYSKVPKINECELSEKFVRGSGPG 444
           ++TI  SK+PK+ E E  +K  +  G G
Sbjct: 221 QNTIYESKIPKLIEHETIQKIAKSKGEG 248


>SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 920

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 16/56 (28%), Positives = 27/56 (48%)
 Frame = +1

Query: 535 DDNRKKARELLIEKLDDMINGPESVSAHRKLIEEKKYKRNATKKQKISKLKDEWKK 702
           DDN K +   L   ++D  NG + VSA + +   K  +  +  ++   KL +  KK
Sbjct: 638 DDNFKVSFMFLDIPIEDKSNGSKGVSATKDVSNGKPAETKSKARKFFDKLFNRRKK 693


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,851,439
Number of Sequences: 5004
Number of extensions: 58574
Number of successful extensions: 197
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)