Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0452
(702 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g36170.2 68418.m04359 peptide chain release factor, putative ... 49 3e-06
At5g36170.1 68418.m04358 peptide chain release factor, putative ... 49 3e-06
At1g56350.1 68414.m06479 peptide chain release factor, putative ... 48 5e-06
At5g36170.3 68418.m04360 peptide chain release factor, putative ... 43 2e-04
At2g47020.2 68415.m05874 peptide chain release factor, putative ... 42 3e-04
At2g47020.1 68415.m05875 peptide chain release factor, putative ... 42 3e-04
At1g33330.1 68414.m04123 peptide chain release factor, putative ... 42 3e-04
At3g62910.1 68416.m07067 peptide chain release factor, putative ... 38 0.005
At1g60070.1 68414.m06767 gamma-adaptin, putative similar to gamm... 32 0.32
At5g67240.1 68418.m08475 exonuclease family protein contains exo... 32 0.42
At5g20040.2 68418.m02385 tRNA isopentenyltransferase 9 / IPP tra... 32 0.42
At5g20040.1 68418.m02384 tRNA isopentenyltransferase 9 / IPP tra... 32 0.42
At1g03830.1 68414.m00364 guanylate-binding family protein contai... 31 0.56
At5g07660.1 68418.m00877 structural maintenance of chromosomes (... 31 0.74
At5g61220.1 68418.m07679 complex 1 family protein / LVR family p... 30 1.7
At2g26780.1 68415.m03212 expressed protein contains Pfam profile... 29 2.3
At2g39725.2 68415.m04876 complex 1 family protein / LVR family p... 29 3.0
At2g39725.1 68415.m04875 complex 1 family protein / LVR family p... 29 3.0
At2g18220.1 68415.m02123 expressed protein contains Pfam domain ... 29 3.0
At2g42250.1 68415.m05230 cytochrome P450 family protein similar ... 29 3.9
At5g67170.2 68418.m08468 SEC-C motif-containing protein / OTU-li... 28 6.9
At5g67170.1 68418.m08467 SEC-C motif-containing protein / OTU-li... 28 6.9
At1g17560.1 68414.m02163 ribosomal protein L14 family protein si... 28 6.9
>At5g36170.2 68418.m04359 peptide chain release factor, putative
similar to SP|P28367 Peptide chain release factor 2
(RF-2) {Bacillus subtilis}; contains Pfam profiles
PF00472: Peptidyl-tRNA hydrolase domain, PF03462: PCRF
domain
Length = 455
Score = 49.2 bits (112), Expect = 3e-06
Identities = 26/63 (41%), Positives = 33/63 (52%)
Frame = +1
Query: 391 KINECELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHTSRCQDDNRKKARELLI 570
+I E +L F R G GG VNK V +THIPTG ++C R Q N+ +A L
Sbjct: 312 EIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLK 371
Query: 571 EKL 579
KL
Sbjct: 372 AKL 374
>At5g36170.1 68418.m04358 peptide chain release factor, putative
similar to SP|P28367 Peptide chain release factor 2
(RF-2) {Bacillus subtilis}; contains Pfam profiles
PF00472: Peptidyl-tRNA hydrolase domain, PF03462: PCRF
domain
Length = 456
Score = 49.2 bits (112), Expect = 3e-06
Identities = 26/63 (41%), Positives = 33/63 (52%)
Frame = +1
Query: 391 KINECELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHTSRCQDDNRKKARELLI 570
+I E +L F R G GG VNK V +THIPTG ++C R Q N+ +A L
Sbjct: 313 EIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLK 372
Query: 571 EKL 579
KL
Sbjct: 373 AKL 375
>At1g56350.1 68414.m06479 peptide chain release factor, putative
similar to SP|P28353 Peptide chain release factor 2
(RF-2). {Salmonella typhi}; contains Pfam profiles
PF00472: Peptidyl-tRNA hydrolase domain, PF03462: PCRF
domain
Length = 482
Score = 48.4 bits (110), Expect = 5e-06
Identities = 24/72 (33%), Positives = 34/72 (47%)
Frame = +1
Query: 373 DYSKVPKINECELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHTSRCQDDNRKK 552
D S +IN+ +L + R G GG N + V + HIPTG C R Q N+
Sbjct: 336 DGSTRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKAS 395
Query: 553 ARELLIEKLDDM 588
A +L +LD +
Sbjct: 396 AMAVLQSRLDQL 407
>At5g36170.3 68418.m04360 peptide chain release factor, putative
similar to SP|P28367 Peptide chain release factor 2
(RF-2) {Bacillus subtilis}; contains Pfam profiles
PF00472: Peptidyl-tRNA hydrolase domain, PF03462: PCRF
domain
Length = 391
Score = 43.2 bits (97), Expect = 2e-04
Identities = 19/42 (45%), Positives = 24/42 (57%)
Frame = +1
Query: 391 KINECELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKC 516
+I E +L F R G GG VNK V +THIPTG ++C
Sbjct: 313 EIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRC 354
>At2g47020.2 68415.m05874 peptide chain release factor, putative
similar to peptide chain release factor 1 [Escherichia
coli] GI:147567; contains Pfam profiles PF00472:
Peptidyl-tRNA hydrolase domain, PF03462: PCRF domain
Length = 348
Score = 42.3 bits (95), Expect = 3e-04
Identities = 27/90 (30%), Positives = 41/90 (45%)
Frame = +1
Query: 310 LTSKYFRNFSPRLAAAFKHTIDYSKVPKINECELSEKFVRGSGPGGSAVNKNSNCVVLTH 489
+T K R + ++ A D V NE +L R G GG N ++ V + H
Sbjct: 177 ITEKSGRIHTSAISVAILPQADEVDVQLRNE-DLRIDTYRSGGSGGQHANTTNSAVRIIH 235
Query: 490 IPTGTVIKCHTSRCQDDNRKKARELLIEKL 579
+PTG ++ R Q NR KA ++L +L
Sbjct: 236 LPTGMMVSIQDERSQHMNRAKALKVLCARL 265
>At2g47020.1 68415.m05875 peptide chain release factor, putative
similar to peptide chain release factor 1 [Escherichia
coli] GI:147567; contains Pfam profiles PF00472:
Peptidyl-tRNA hydrolase domain, PF03462: PCRF domain
Length = 413
Score = 42.3 bits (95), Expect = 3e-04
Identities = 27/90 (30%), Positives = 41/90 (45%)
Frame = +1
Query: 310 LTSKYFRNFSPRLAAAFKHTIDYSKVPKINECELSEKFVRGSGPGGSAVNKNSNCVVLTH 489
+T K R + ++ A D V NE +L R G GG N ++ V + H
Sbjct: 242 ITEKSGRIHTSAISVAILPQADEVDVQLRNE-DLRIDTYRSGGSGGQHANTTNSAVRIIH 300
Query: 490 IPTGTVIKCHTSRCQDDNRKKARELLIEKL 579
+PTG ++ R Q NR KA ++L +L
Sbjct: 301 LPTGMMVSIQDERSQHMNRAKALKVLCARL 330
>At1g33330.1 68414.m04123 peptide chain release factor, putative
similar to peptide chain release factor 2 [Campylobacter
jejuni] GI:6469929; contains Pfam profile PF00472:
Peptidyl-tRNA hydrolase domain
Length = 257
Score = 42.3 bits (95), Expect = 3e-04
Identities = 27/75 (36%), Positives = 35/75 (46%)
Frame = +1
Query: 394 INECELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHTSRCQDDNRKKARELLIE 573
+ +C L E F R SGPGG NK + V L H+PTG V + R Q NR A L
Sbjct: 95 MKQCRL-ETF-RVSGPGGQHRNKRDSAVRLKHLPTGIVAQAVEDRSQHKNRASALNRLRT 152
Query: 574 KLDDMINGPESVSAH 618
L + + A+
Sbjct: 153 LLAIKVRNKVDIEAY 167
>At3g62910.1 68416.m07067 peptide chain release factor, putative
similar to peptide chain release factor 1 [Escherichia
coli] GI:147567; contains Pfam profiles PF00472:
Peptidyl-tRNA hydrolase domain, PF03462: PCRF domain
Length = 422
Score = 38.3 bits (85), Expect = 0.005
Identities = 21/51 (41%), Positives = 26/51 (50%)
Frame = +1
Query: 427 RGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHTSRCQDDNRKKARELLIEKL 579
R G GG VNK + L H P+G I C R Q N+ +A +LL KL
Sbjct: 283 RSGGAGGQNVNKVETAIDLFHKPSGIRIFCTEERTQIRNKARAFQLLRAKL 333
>At1g60070.1 68414.m06767 gamma-adaptin, putative similar to
gamma-adaptin GI:2765190 from [Homo sapiens]; contains
Pfam profiles PF01602: Adaptin N terminal region,
PF02883: Adaptin C-terminal domain
Length = 867
Score = 32.3 bits (70), Expect = 0.32
Identities = 21/67 (31%), Positives = 35/67 (52%)
Frame = +1
Query: 406 ELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHTSRCQDDNRKKARELLIEKLDD 585
+LSE F+ PG + + + + V++T + T I +S + RKK E L++ L D
Sbjct: 173 DLSENFIN---PGAALLKEKHHGVLITGVHLCTEICKVSSEALEYFRKKCTEGLVKTLRD 229
Query: 586 MINGPES 606
+ N P S
Sbjct: 230 IANSPYS 236
>At5g67240.1 68418.m08475 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 745
Score = 31.9 bits (69), Expect = 0.42
Identities = 21/53 (39%), Positives = 28/53 (52%)
Frame = +1
Query: 511 KCHTSRCQDDNRKKARELLIEKLDDMINGPESVSAHRKLIEEKKYKRNATKKQ 669
+ + + C+DD+ K+ EL EKL M S H K IEE K K NA + Q
Sbjct: 671 EANANHCEDDHLKEMEELK-EKLKAM-EFAISCEGHSKEIEELKQKLNAKEHQ 721
>At5g20040.2 68418.m02385 tRNA isopentenyltransferase 9 / IPP
transferase 9 (IPT9) identical to tRNA
isopentenyltransferase (IPT9) [Arabidopsis thaliana]
GI:14279070
Length = 459
Score = 31.9 bits (69), Expect = 0.42
Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = -2
Query: 692 SSFNLDIFCFFVAF-RLYFFSSISFLCADTLSGP 594
+ + D CFF++ R+ + SI F C D LSGP
Sbjct: 264 TDLDYDFLCFFLSSPRVALYRSIDFRCEDMLSGP 297
>At5g20040.1 68418.m02384 tRNA isopentenyltransferase 9 / IPP
transferase 9 (IPT9) identical to tRNA
isopentenyltransferase (IPT9) [Arabidopsis thaliana]
GI:14279070
Length = 463
Score = 31.9 bits (69), Expect = 0.42
Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = -2
Query: 692 SSFNLDIFCFFVAF-RLYFFSSISFLCADTLSGP 594
+ + D CFF++ R+ + SI F C D LSGP
Sbjct: 264 TDLDYDFLCFFLSSPRVALYRSIDFRCEDMLSGP 297
>At1g03830.1 68414.m00364 guanylate-binding family protein contains
Pfam domains PF02263: Guanylate-binding protein,
N-terminal domain and PF02841: Guanylate-binding
protein, C-terminal domain
Length = 991
Score = 31.5 bits (68), Expect = 0.56
Identities = 15/49 (30%), Positives = 29/49 (59%)
Frame = +1
Query: 526 RCQDDNRKKARELLIEKLDDMINGPESVSAHRKLIEEKKYKRNATKKQK 672
RC + +R K+R +EK+ + +G SA +KL++ K + A++K +
Sbjct: 701 RCIEIDRAKSRIEELEKVCTLNSGEGEASASKKLVDSMKMEAEASRKNE 749
>At5g07660.1 68418.m00877 structural maintenance of chromosomes
(SMC) family protein similar to SMC-like protein (MIM)
[Arabidopsis thaliana] GI:5880614; contains Pfam profile
PF02463: RecF/RecN/SMC N terminal domain
Length = 1058
Score = 31.1 bits (67), Expect = 0.74
Identities = 21/81 (25%), Positives = 35/81 (43%)
Frame = +1
Query: 460 KNSNCVVLTHIPTGTVIKCHTSRCQDDNRKKARELLIEKLDDMINGPESVSAHRKLIEEK 639
KNS PT +V + H + + +E L+EKL D + E + K E
Sbjct: 701 KNSVASETKASPTSSVNELHLEIMKFQKEIEEKESLLEKLQDSLKEAELKANELKASYEN 760
Query: 640 KYKRNATKKQKISKLKDEWKK 702
Y+ + + + K +DE K+
Sbjct: 761 LYESAKGEIEALEKAEDELKE 781
>At5g61220.1 68418.m07679 complex 1 family protein / LVR family
protein contains Pfam PF05347: Complex 1 protein (LYR
family)
Length = 87
Score = 29.9 bits (64), Expect = 1.7
Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = +3
Query: 102 ITRKEVLRLYRNLLMYSKSLK-LTDPDYFRRSIKIEFKNNQNLTDLEKISFAYQKGQALL 278
++ EVL L R LL + +Y +R F+ N+NLTD K++ AY + + L
Sbjct: 2 VSSSEVLSLCRALLRAGRQFPDYNIREYSKRRTLDGFRMNKNLTDPSKVTEAYAEAKKQL 61
>At2g26780.1 68415.m03212 expressed protein contains Pfam profile TBP
(TATA-binding protein) -interacting protein 120 (TIP120);
contains TIGRFAM profile TIGR01612: reticulocyte binding
protein
Length = 1866
Score = 29.5 bits (63), Expect = 2.3
Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +1
Query: 286 GQLCDMF-FLTSKYFRNFSPRLAAAFKHTIDYSKVPKINEC 405
G+LC F L + + SP A F H + +S VPK+ EC
Sbjct: 1755 GKLCSRFPSLWTDSMDDLSPSDATKFVHELFHSLVPKLLEC 1795
>At2g39725.2 68415.m04876 complex 1 family protein / LVR family
protein contains Pfam PF05347: Complex 1 protein (LYR
family)
Length = 90
Score = 29.1 bits (62), Expect = 3.0
Identities = 13/48 (27%), Positives = 23/48 (47%)
Frame = +3
Query: 108 RKEVLRLYRNLLMYSKSLKLTDPDYFRRSIKIEFKNNQNLTDLEKISF 251
+K+VL LYR L ++S + D + EF++N D + +
Sbjct: 10 QKQVLSLYRGFLRAARSRPIEDRKRIEMIVSTEFRHNSKEVDRKNFQY 57
>At2g39725.1 68415.m04875 complex 1 family protein / LVR family
protein contains Pfam PF05347: Complex 1 protein (LYR
family)
Length = 90
Score = 29.1 bits (62), Expect = 3.0
Identities = 13/48 (27%), Positives = 23/48 (47%)
Frame = +3
Query: 108 RKEVLRLYRNLLMYSKSLKLTDPDYFRRSIKIEFKNNQNLTDLEKISF 251
+K+VL LYR L ++S + D + EF++N D + +
Sbjct: 10 QKQVLSLYRGFLRAARSRPIEDRKRIEMIVSTEFRHNSKEVDRKNFQY 57
>At2g18220.1 68415.m02123 expressed protein contains Pfam domain
PF03715: Uncharacterised protein family (UPF0120)
Length = 779
Score = 29.1 bits (62), Expect = 3.0
Identities = 16/54 (29%), Positives = 32/54 (59%), Gaps = 2/54 (3%)
Frame = +1
Query: 538 DNRKKARELLIEKLD--DMINGPESVSAHRKLIEEKKYKRNATKKQKISKLKDE 693
D++K+ ++L +KL+ + +N + + AH ++E+K K+ KK K K + E
Sbjct: 20 DDKKRVKKLKSKKLEAEEELNNVQEIDAHDIVMEQKSDKKRG-KKVKSKKAEAE 72
>At2g42250.1 68415.m05230 cytochrome P450 family protein similar to
cytochrome P450 93A1 (SP:Q42798) [Glycine max]
Length = 514
Score = 28.7 bits (61), Expect = 3.9
Identities = 15/49 (30%), Positives = 24/49 (48%)
Frame = +1
Query: 256 IRKVRPCCREGQLCDMFFLTSKYFRNFSPRLAAAFKHTIDYSKVPKINE 402
+ V CCREG CD+ KY N R+A + + + ++ +I E
Sbjct: 165 VDSVAKCCREGLPCDLSSQFIKYTNNVICRMAMSTRCSGTDNEAEEIRE 213
>At5g67170.2 68418.m08468 SEC-C motif-containing protein / OTU-like
cysteine protease family protein contains Pfam profiles
PF02338: OTU-like cysteine protease, PF02810: SEC-C
motif
Length = 374
Score = 27.9 bits (59), Expect = 6.9
Identities = 19/54 (35%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +1
Query: 502 TVIKCHTSRCQDDNRKKARELLIEKLDDMINGPESVSAH-RKLIEEKKYKRNAT 660
T+ H S +N ++ARE LIE+ N E+V A ++KK RN T
Sbjct: 262 TINPKHASDSPMENTEQAREELIEEESASGNNSETVQAKCTTQTDDKKIPRNKT 315
>At5g67170.1 68418.m08467 SEC-C motif-containing protein / OTU-like
cysteine protease family protein contains Pfam profiles
PF02338: OTU-like cysteine protease, PF02810: SEC-C
motif
Length = 375
Score = 27.9 bits (59), Expect = 6.9
Identities = 19/54 (35%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +1
Query: 502 TVIKCHTSRCQDDNRKKARELLIEKLDDMINGPESVSAH-RKLIEEKKYKRNAT 660
T+ H S +N ++ARE LIE+ N E+V A ++KK RN T
Sbjct: 263 TINPKHASDSPMENTEQAREELIEEESASGNNSETVQAKCTTQTDDKKIPRNKT 316
>At1g17560.1 68414.m02163 ribosomal protein L14 family protein
similar to GB:Z98756 from (Mycobacterium leprae)
Length = 196
Score = 27.9 bits (59), Expect = 6.9
Identities = 21/68 (30%), Positives = 34/68 (50%), Gaps = 2/68 (2%)
Frame = +1
Query: 484 THIPTGTVIKCHTSRCQDDNR--KKARELLIEKLDDMINGPESVSAHRKLIEEKKYKRNA 657
T I GT++KC + C + + R +L D+I G SV ++ +KK K++A
Sbjct: 47 TFIQMGTILKCVDNSCAKEVMCIQSLRGKKGARLGDIIVG--SVKEANPIV-QKKVKKDA 103
Query: 658 TKKQKISK 681
K K+ K
Sbjct: 104 IPKGKVKK 111
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,387,974
Number of Sequences: 28952
Number of extensions: 292706
Number of successful extensions: 871
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 837
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 871
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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