Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0446
(716 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g06990.1 68415.m00800 HUA enhancer 2 (HEN2) / DExH-box RNA he... 31 0.76
At5g65090.1 68418.m08187 endonuclease/exonuclease/phosphatase fa... 29 2.3
At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP... 29 3.1
At3g03970.3 68416.m00418 expressed protein 29 3.1
At3g03970.2 68416.m00417 expressed protein 29 3.1
At3g03970.1 68416.m00416 expressed protein 29 3.1
At4g20770.1 68417.m03016 pentatricopeptide (PPR) repeat-containi... 29 4.1
At2g36790.1 68415.m04512 UDP-glucoronosyl/UDP-glucosyl transfera... 28 5.4
At1g80630.1 68414.m09462 leucine-rich repeat family protein 28 5.4
At5g53870.1 68418.m06701 plastocyanin-like domain-containing pro... 28 7.1
At5g19090.2 68418.m02270 heavy-metal-associated domain-containin... 28 7.1
At5g19090.1 68418.m02269 heavy-metal-associated domain-containin... 28 7.1
At1g20760.1 68414.m02600 calcium-binding EF hand family protein ... 28 7.1
At5g09950.1 68418.m01150 pentatricopeptide (PPR) repeat-containi... 27 9.4
At3g53240.1 68416.m05868 leucine-rich repeat family protein cont... 27 9.4
At3g02540.2 68416.m00243 ubiquitin family protein contains Pfam ... 27 9.4
At3g02540.1 68416.m00242 ubiquitin family protein contains Pfam ... 27 9.4
At1g55900.1 68414.m06411 NLI interacting factor (NIF) family pro... 27 9.4
>At2g06990.1 68415.m00800 HUA enhancer 2 (HEN2) / DExH-box RNA
helicase, putative nearly identical to HUA enhancer 2
[Arabidopsis thaliana] GI:16024936
Length = 995
Score = 31.1 bits (67), Expect = 0.76
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 359 KGSVLKWARVIDEFLNQYRKKGSGSGEA 442
+GS+++ AR +DEFLNQ R GE+
Sbjct: 943 EGSIIRSARRLDEFLNQLRAAAEAVGES 970
>At5g65090.1 68418.m08187 endonuclease/exonuclease/phosphatase
family protein similar to inositol polyphosphate
5-phosphatase I (GI:10444261) and II (GI:10444263)
[Arabidopsis thaliana]; contains Pfam profile PF03372:
Endonuclease/Exonuclease/phosphatase family
Length = 529
Score = 29.5 bits (63), Expect = 2.3
Identities = 14/44 (31%), Positives = 21/44 (47%)
Frame = +2
Query: 350 VNDKGSVLKWARVIDEFLNQYRKKGSGSGEAHGAENRVPCSPSS 481
V D KW +I + LN+ +++ S A+ A CS SS
Sbjct: 124 VEDNEPAAKWLALISQALNKPKQESVYSNAAYSASRTTTCSSSS 167
>At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP2)
identical to biotin carboxyl carrier protein isoform 2
[Arabidopsis thaliana] gi|8886869|gb|AAF80592
Length = 255
Score = 29.1 bits (62), Expect = 3.1
Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = +3
Query: 414 VRKAAAVVKLMVPKTAYHALPAADHSARSKSVMYQSMTSIPALQPTST-ITNKPALASSS 590
+RK A+ + + P YH++P A Q + P+ PTST T KP A SS
Sbjct: 119 IRKKEALQQAVPPAPVYHSMPPV--MADFSMPPAQPVALPPSPTPTSTPATAKPTSAPSS 176
Query: 591 S 593
S
Sbjct: 177 S 177
>At3g03970.3 68416.m00418 expressed protein
Length = 554
Score = 29.1 bits (62), Expect = 3.1
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +3
Query: 582 SSSSSIRYTTGRPQPYNNTEDLPTNMPEDLKQHIK 686
SSSSSI T+G P N TED+ DL K
Sbjct: 467 SSSSSIYDTSGTTTPTNTTEDICEKPKTDLDSEAK 501
>At3g03970.2 68416.m00417 expressed protein
Length = 554
Score = 29.1 bits (62), Expect = 3.1
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +3
Query: 582 SSSSSIRYTTGRPQPYNNTEDLPTNMPEDLKQHIK 686
SSSSSI T+G P N TED+ DL K
Sbjct: 467 SSSSSIYDTSGTTTPTNTTEDICEKPKTDLDSEAK 501
>At3g03970.1 68416.m00416 expressed protein
Length = 554
Score = 29.1 bits (62), Expect = 3.1
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +3
Query: 582 SSSSSIRYTTGRPQPYNNTEDLPTNMPEDLKQHIK 686
SSSSSI T+G P N TED+ DL K
Sbjct: 467 SSSSSIYDTSGTTTPTNTTEDICEKPKTDLDSEAK 501
>At4g20770.1 68417.m03016 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 740
Score = 28.7 bits (61), Expect = 4.1
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -1
Query: 485 VRCWESMVRGFRHHEL 438
+ CW SM+ GFRH+ L
Sbjct: 449 IACWNSMISGFRHNML 464
>At2g36790.1 68415.m04512 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 495
Score = 28.3 bits (60), Expect = 5.4
Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 4/41 (9%)
Frame = -2
Query: 622 CGLPVVYLIEL----EEDARAGLFVIVLVGWSAGIEVIDWY 512
C LP+ L+EL EE R F+ V+ GW E+++W+
Sbjct: 298 CNLPLSQLLELGLGLEESQRP--FIWVIRGWEKYKELVEWF 336
>At1g80630.1 68414.m09462 leucine-rich repeat family protein
Length = 578
Score = 28.3 bits (60), Expect = 5.4
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = -3
Query: 336 RVLSTLAVSGIGLKPRPGLL 277
R L +LA++GIGL+PR LL
Sbjct: 215 RNLESLAINGIGLRPRESLL 234
>At5g53870.1 68418.m06701 plastocyanin-like domain-containing
protein contains similarity to SP|Q02917 Early nodulin
55-2 precursor {Glycine max}; PF02298: Plastocyanin-like
domain
Length = 370
Score = 27.9 bits (59), Expect = 7.1
Identities = 25/79 (31%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = +3
Query: 450 PKTAYHALPAADHSARSKSVMYQSMTSIPALQP--TSTITNKPALASSSSSIRYTTGRPQ 623
PK+ P A SA SKS +S S PA P +S I++ PAL S S + ++ P
Sbjct: 151 PKSHSPVSPVAPASAPSKSQPPRSSVS-PAQPPKSSSPISHTPAL-SPSHATSHSPATPS 208
Query: 624 PYNNTEDLPTNMPEDLKQH 680
P + ++ P H
Sbjct: 209 PSPKSPSPVSHSPSHSPAH 227
>At5g19090.2 68418.m02270 heavy-metal-associated domain-containing
protein contains Pfam heavy-metal-associated domain
PF00403; glycine-rich protein GRP22, rape, PIR:S31415;
isoform contains a non-consensus TG-acceptor splice site
at intron 3
Length = 465
Score = 27.9 bits (59), Expect = 7.1
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
Frame = +1
Query: 67 REMGGVQDL--PVQLGDGPGPRAHRLKLGKDP 156
++M G QDL P QL GPGP + + K+P
Sbjct: 168 QQMKGFQDLKLPPQLKGGPGPGPGSVPMNKNP 199
>At5g19090.1 68418.m02269 heavy-metal-associated domain-containing
protein contains Pfam heavy-metal-associated domain
PF00403; glycine-rich protein GRP22, rape, PIR:S31415;
isoform contains a non-consensus TG-acceptor splice site
at intron 3
Length = 587
Score = 27.9 bits (59), Expect = 7.1
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
Frame = +1
Query: 67 REMGGVQDL--PVQLGDGPGPRAHRLKLGKDP 156
++M G QDL P QL GPGP + + K+P
Sbjct: 168 QQMKGFQDLKLPPQLKGGPGPGPGSVPMNKNP 199
>At1g20760.1 68414.m02600 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 1019
Score = 27.9 bits (59), Expect = 7.1
Identities = 14/43 (32%), Positives = 20/43 (46%)
Frame = +2
Query: 428 GSGEAHGAENRVPCSPSSGPLGEKQVCDVPVDDFNPCTPANQY 556
GSG+ G SPSS G ++ DD P TP +++
Sbjct: 833 GSGDDFGGNTARADSPSSRSFGAQRKSQFAFDDSVPSTPLSRF 875
>At5g09950.1 68418.m01150 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 995
Score = 27.5 bits (58), Expect = 9.4
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -1
Query: 515 VHHRLASRRVVRCWESMVRGFRHHEL 438
+ R+A RR W SM+ G+ H+EL
Sbjct: 574 IFSRMAERRDNVTWNSMISGYIHNEL 599
>At3g53240.1 68416.m05868 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 891
Score = 27.5 bits (58), Expect = 9.4
Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = -2
Query: 595 ELEEDARAGLFVIVLVGWSAGIEVIDWYITDLL-LAEWSAAGRAWYAVFGT 446
E E+D +GL IV++ WS G + + L+ L S RAW+ + T
Sbjct: 829 EYEDDDESGLLDIVVLWWSLGTTYVTVMMGFLVFLCFDSPWRRAWFCLVDT 879
>At3g02540.2 68416.m00243 ubiquitin family protein contains Pfam
profiles PF00240: Ubiquitin family, PF00627: UBA/TS-N
domain;
Length = 299
Score = 27.5 bits (58), Expect = 9.4
Identities = 23/103 (22%), Positives = 45/103 (43%), Gaps = 2/103 (1%)
Frame = +3
Query: 408 NTVRKAAAVVKLMVPKTAYHALPAADHSARSKSVMYQSMTSIPALQPTS--TITNKPALA 581
N V + + +V +M A ++ + S++ TS P++ P + +++ A A
Sbjct: 62 NKVAENSFIVIMMNKSKPASAAASSASAGTSQAKSIPPSTSQPSISPQTPASVSAPVAPA 121
Query: 582 SSSSSIRYTTGRPQPYNNTEDLPTNMPEDLKQHIKTLSGKPDA 710
+ T P P TE + T +PE + I + + PD+
Sbjct: 122 PTRPPPPAPTPTPAPVAATETVTTPIPEPVPATISSSTPAPDS 164
>At3g02540.1 68416.m00242 ubiquitin family protein contains Pfam
profiles PF00240: Ubiquitin family, PF00627: UBA/TS-N
domain;
Length = 419
Score = 27.5 bits (58), Expect = 9.4
Identities = 23/103 (22%), Positives = 45/103 (43%), Gaps = 2/103 (1%)
Frame = +3
Query: 408 NTVRKAAAVVKLMVPKTAYHALPAADHSARSKSVMYQSMTSIPALQPTS--TITNKPALA 581
N V + + +V +M A ++ + S++ TS P++ P + +++ A A
Sbjct: 62 NKVAENSFIVIMMNKSKPASAAASSASAGTSQAKSIPPSTSQPSISPQTPASVSAPVAPA 121
Query: 582 SSSSSIRYTTGRPQPYNNTEDLPTNMPEDLKQHIKTLSGKPDA 710
+ T P P TE + T +PE + I + + PD+
Sbjct: 122 PTRPPPPAPTPTPAPVAATETVTTPIPEPVPATISSSTPAPDS 164
>At1g55900.1 68414.m06411 NLI interacting factor (NIF) family
protein contains Pfam profile PF03031: NLI interacting
factor
Length = 376
Score = 27.5 bits (58), Expect = 9.4
Identities = 26/104 (25%), Positives = 42/104 (40%), Gaps = 2/104 (1%)
Frame = -2
Query: 541 SAGIEVIDWYITDLLLAEWSAAGRA--WYAVFGTMSFTTAAAFLTVLIQKFVNNPGPFEH 368
S+GI+VID Y T L A + + RA Y + F L +K + + P E
Sbjct: 129 SSGIDVIDKYQTKLYSAAMTGSARAIDKYLELREIVEEQVKGFTEPLSEKLLPDLHPAEQ 188
Query: 367 RSLIVDLVIDQSTFHVGSIWHWPEAQTWITADY*TIKLPLRHLG 236
+ L ++++ + W + W T + L HLG
Sbjct: 189 HVFTLVLDLNETLLYT----DWKRERGWRTFKRPGVDAFLEHLG 228
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,028,031
Number of Sequences: 28952
Number of extensions: 387519
Number of successful extensions: 1206
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1205
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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