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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0436
         (573 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   233   1e-62
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   229   2e-61
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    95   5e-21
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    52   5e-08
SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces po...    28   0.84 
SPBC1685.07c |||amino acid transporter |Schizosaccharomyces pomb...    27   2.0  
SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family |Schizosa...    27   2.6  
SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces po...    27   2.6  
SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizo...    25   6.0  
SPAC1006.05c |och1||alpha-1,6-mannosyltransferase Och1 |Schizosa...    25   6.0  
SPAC22E12.11c |set3||histone lysine methyltransferase Set3|Schiz...    25   7.9  

>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  233 bits (571), Expect = 1e-62
 Identities = 99/125 (79%), Positives = 113/125 (90%)
 Frame = +3

Query: 3   TNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGF 182
           TN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGF
Sbjct: 296 TNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGF 355

Query: 183 KVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGE 362
           K+GI Y+PP  VPG  +AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGE
Sbjct: 356 KIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGE 415

Query: 363 GMEEG 377
           GMEEG
Sbjct: 416 GMEEG 420



 Score = 52.0 bits (119), Expect = 6e-08
 Identities = 24/33 (72%), Positives = 28/33 (84%)
 Frame = +2

Query: 380 EFSEAREDLAALEKDYEEVGVDSTEGELDEENE 478
           EFSEAREDLAALE+DYEEVG DS + E+ E +E
Sbjct: 421 EFSEAREDLAALERDYEEVGQDSMDNEMYEADE 453


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  229 bits (560), Expect = 2e-61
 Identities = 98/125 (78%), Positives = 111/125 (88%)
 Frame = +3

Query: 3   TNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGF 182
           TN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGF
Sbjct: 292 TNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGF 351

Query: 183 KVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGE 362
           K+GI  +PP  + G ++AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGE
Sbjct: 352 KIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGE 411

Query: 363 GMEEG 377
           GMEEG
Sbjct: 412 GMEEG 416



 Score = 50.0 bits (114), Expect = 2e-07
 Identities = 26/34 (76%), Positives = 28/34 (82%)
 Frame = +2

Query: 380 EFSEAREDLAALEKDYEEVGVDSTEGELDEENEY 481
           EFSEAREDLAALE+DYEEVG DS E +  EE EY
Sbjct: 417 EFSEAREDLAALERDYEEVGQDSMEVDYMEE-EY 449


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 95.5 bits (227), Expect = 5e-21
 Identities = 46/124 (37%), Positives = 70/124 (56%)
 Frame = +3

Query: 3   TNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGF 182
           T   F+  N MV  DPRHG+Y+    L+RG V  K+V+  I +++TK +  FV+W P   
Sbjct: 290 TQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNV 349

Query: 183 KVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGE 362
              +   PP      DL   + +   + N+T+I E + RL  +F  M+ ++AF+HWY GE
Sbjct: 350 LKAVCSVPPK-----DL---KMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGE 401

Query: 363 GMEE 374
           GM+E
Sbjct: 402 GMDE 405


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 52.4 bits (120), Expect = 5e-08
 Identities = 30/119 (25%), Positives = 61/119 (51%), Gaps = 1/119 (0%)
 Frame = +3

Query: 21  PANQMVKCDP-RHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGIN 197
           P NQMV  +P +   +++   + +G+  P DV+ ++  I+ +R   F+ W P   +V ++
Sbjct: 302 PKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALS 361

Query: 198 YQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE 374
            + P +     ++ +     ML+N T+IA  + R   ++D +  + AF+  Y  E + E
Sbjct: 362 KKSPYIKTNHRVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFE 415


>SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 412

 Score = 28.3 bits (60), Expect = 0.84
 Identities = 23/89 (25%), Positives = 41/89 (46%), Gaps = 3/89 (3%)
 Frame = -2

Query: 335 ALSVHQVELV-VQSGPRLGDGGCVGQHAHGALHLGEVSARHHCWGLVIDAHLETSWAPVH 159
           A SV  VE+V  Q+   L DG  +        H GE     + + +++  ++E+ +  V 
Sbjct: 89  ASSVSPVEIVPYQTDIILEDGTKINLTVLDTPHFGEAIDNENNFDIILQ-YIESQYDNVL 147

Query: 158 ELDGSLGFDG--CDSRIHVLRHNVSSVQH 78
           E +  +  +   CD R+H L + +S   H
Sbjct: 148 EEESRIKRNARFCDDRVHALIYFISPTGH 176


>SPBC1685.07c |||amino acid transporter |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 420

 Score = 27.1 bits (57), Expect = 2.0
 Identities = 19/48 (39%), Positives = 27/48 (56%)
 Frame = -3

Query: 145 LLVLMVAIAAFTSFGTTSPLYSIQQAMYFP*RGSHFTIWLAGSKHALV 2
           LL L+VAI  + SFG+ +    I  AMY      + +IW+ G K A+V
Sbjct: 250 LLYLLVAITGYLSFGSLASGNII--AMY-----DNTSIWIIGGKLAIV 290


>SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 707

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 12/37 (32%), Positives = 18/37 (48%), Gaps = 1/37 (2%)
 Frame = +1

Query: 121 LSQPSKP-REPSSSWTGAQLVSRWASITNPQQWCRAE 228
           + QPSK  R+P  SW+    +  WA + +   W   E
Sbjct: 417 IHQPSKDSRKPVQSWSYVHSLDFWARLLSQAAWLSRE 453


>SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 284

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 16/70 (22%), Positives = 30/70 (42%)
 Frame = -2

Query: 281 DGGCVGQHAHGALHLGEVSARHHCWGLVIDAHLETSWAPVHELDGSLGFDGCDSRIHVLR 102
           +G  +G+  HG +H  +VS +    G+   A  +  W+ + E +   G    D   + + 
Sbjct: 40  NGNGLGEKQHGRIHNIKVSLKDDTLGIGAKATNDLEWSGLGEFNAIFGRLNGDESAYGVY 99

Query: 101 HNVSSVQHTT 72
              + VQ  T
Sbjct: 100 AEKAKVQQLT 109


>SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit
           Bgs3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1826

 Score = 25.4 bits (53), Expect = 6.0
 Identities = 10/28 (35%), Positives = 13/28 (46%)
 Frame = -1

Query: 84  TAYNKPCIFHDAGHISPSGWRARSTRWC 1
           T YN P I+    H       AR+ +WC
Sbjct: 439 TVYNSPTIYTPNFHYLEGTQPARAAKWC 466


>SPAC1006.05c |och1||alpha-1,6-mannosyltransferase Och1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 396

 Score = 25.4 bits (53), Expect = 6.0
 Identities = 16/42 (38%), Positives = 21/42 (50%)
 Frame = +2

Query: 389 EAREDLAALEKDYEEVGVDSTEGELDEENEY*SGMKNSRSVL 514
           E   DL A   D E   ++ +  ELDEEN Y   ++ S S L
Sbjct: 72  EEDPDLEAYLSDLEREELEHSLEELDEENNYKLHLRYSFSQL 113


>SPAC22E12.11c |set3||histone lysine methyltransferase
           Set3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 859

 Score = 25.0 bits (52), Expect = 7.9
 Identities = 10/29 (34%), Positives = 18/29 (62%)
 Frame = +2

Query: 452 EGELDEENEY*SGMKNSRSVLGRKEPNSY 538
           +G +  +NEY S  KN  ++LG  +P+ +
Sbjct: 245 KGRVCTQNEYKSDPKNQYNILGAPKPHVF 273


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,339,666
Number of Sequences: 5004
Number of extensions: 47236
Number of successful extensions: 156
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 244081442
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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