BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0436 (573 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 233 1e-62 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 229 2e-61 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 95 5e-21 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 52 5e-08 SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces po... 28 0.84 SPBC1685.07c |||amino acid transporter |Schizosaccharomyces pomb... 27 2.0 SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family |Schizosa... 27 2.6 SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces po... 27 2.6 SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizo... 25 6.0 SPAC1006.05c |och1||alpha-1,6-mannosyltransferase Och1 |Schizosa... 25 6.0 SPAC22E12.11c |set3||histone lysine methyltransferase Set3|Schiz... 25 7.9 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 233 bits (571), Expect = 1e-62 Identities = 99/125 (79%), Positives = 113/125 (90%) Frame = +3 Query: 3 TNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGF 182 TN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGF Sbjct: 296 TNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGF 355 Query: 183 KVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGE 362 K+GI Y+PP VPG +AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGE Sbjct: 356 KIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGE 415 Query: 363 GMEEG 377 GMEEG Sbjct: 416 GMEEG 420 Score = 52.0 bits (119), Expect = 6e-08 Identities = 24/33 (72%), Positives = 28/33 (84%) Frame = +2 Query: 380 EFSEAREDLAALEKDYEEVGVDSTEGELDEENE 478 EFSEAREDLAALE+DYEEVG DS + E+ E +E Sbjct: 421 EFSEAREDLAALERDYEEVGQDSMDNEMYEADE 453 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 229 bits (560), Expect = 2e-61 Identities = 98/125 (78%), Positives = 111/125 (88%) Frame = +3 Query: 3 TNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGF 182 TN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGF Sbjct: 292 TNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGF 351 Query: 183 KVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGE 362 K+GI +PP + G ++AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGE Sbjct: 352 KIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGE 411 Query: 363 GMEEG 377 GMEEG Sbjct: 412 GMEEG 416 Score = 50.0 bits (114), Expect = 2e-07 Identities = 26/34 (76%), Positives = 28/34 (82%) Frame = +2 Query: 380 EFSEAREDLAALEKDYEEVGVDSTEGELDEENEY 481 EFSEAREDLAALE+DYEEVG DS E + EE EY Sbjct: 417 EFSEAREDLAALERDYEEVGQDSMEVDYMEE-EY 449 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 95.5 bits (227), Expect = 5e-21 Identities = 46/124 (37%), Positives = 70/124 (56%) Frame = +3 Query: 3 TNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGF 182 T F+ N MV DPRHG+Y+ L+RG V K+V+ I +++TK + FV+W P Sbjct: 290 TQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNV 349 Query: 183 KVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGE 362 + PP DL + + + N+T+I E + RL +F M+ ++AF+HWY GE Sbjct: 350 LKAVCSVPPK-----DL---KMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGE 401 Query: 363 GMEE 374 GM+E Sbjct: 402 GMDE 405 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 52.4 bits (120), Expect = 5e-08 Identities = 30/119 (25%), Positives = 61/119 (51%), Gaps = 1/119 (0%) Frame = +3 Query: 21 PANQMVKCDP-RHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGIN 197 P NQMV +P + +++ + +G+ P DV+ ++ I+ +R F+ W P +V ++ Sbjct: 302 PKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALS 361 Query: 198 YQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE 374 + P + ++ + ML+N T+IA + R ++D + + AF+ Y E + E Sbjct: 362 KKSPYIKTNHRVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFE 415 >SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces pombe|chr 2|||Manual Length = 412 Score = 28.3 bits (60), Expect = 0.84 Identities = 23/89 (25%), Positives = 41/89 (46%), Gaps = 3/89 (3%) Frame = -2 Query: 335 ALSVHQVELV-VQSGPRLGDGGCVGQHAHGALHLGEVSARHHCWGLVIDAHLETSWAPVH 159 A SV VE+V Q+ L DG + H GE + + +++ ++E+ + V Sbjct: 89 ASSVSPVEIVPYQTDIILEDGTKINLTVLDTPHFGEAIDNENNFDIILQ-YIESQYDNVL 147 Query: 158 ELDGSLGFDG--CDSRIHVLRHNVSSVQH 78 E + + + CD R+H L + +S H Sbjct: 148 EEESRIKRNARFCDDRVHALIYFISPTGH 176 >SPBC1685.07c |||amino acid transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 420 Score = 27.1 bits (57), Expect = 2.0 Identities = 19/48 (39%), Positives = 27/48 (56%) Frame = -3 Query: 145 LLVLMVAIAAFTSFGTTSPLYSIQQAMYFP*RGSHFTIWLAGSKHALV 2 LL L+VAI + SFG+ + I AMY + +IW+ G K A+V Sbjct: 250 LLYLLVAITGYLSFGSLASGNII--AMY-----DNTSIWIIGGKLAIV 290 >SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 707 Score = 26.6 bits (56), Expect = 2.6 Identities = 12/37 (32%), Positives = 18/37 (48%), Gaps = 1/37 (2%) Frame = +1 Query: 121 LSQPSKP-REPSSSWTGAQLVSRWASITNPQQWCRAE 228 + QPSK R+P SW+ + WA + + W E Sbjct: 417 IHQPSKDSRKPVQSWSYVHSLDFWARLLSQAAWLSRE 453 >SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 284 Score = 26.6 bits (56), Expect = 2.6 Identities = 16/70 (22%), Positives = 30/70 (42%) Frame = -2 Query: 281 DGGCVGQHAHGALHLGEVSARHHCWGLVIDAHLETSWAPVHELDGSLGFDGCDSRIHVLR 102 +G +G+ HG +H +VS + G+ A + W+ + E + G D + + Sbjct: 40 NGNGLGEKQHGRIHNIKVSLKDDTLGIGAKATNDLEWSGLGEFNAIFGRLNGDESAYGVY 99 Query: 101 HNVSSVQHTT 72 + VQ T Sbjct: 100 AEKAKVQQLT 109 >SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1826 Score = 25.4 bits (53), Expect = 6.0 Identities = 10/28 (35%), Positives = 13/28 (46%) Frame = -1 Query: 84 TAYNKPCIFHDAGHISPSGWRARSTRWC 1 T YN P I+ H AR+ +WC Sbjct: 439 TVYNSPTIYTPNFHYLEGTQPARAAKWC 466 >SPAC1006.05c |och1||alpha-1,6-mannosyltransferase Och1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 396 Score = 25.4 bits (53), Expect = 6.0 Identities = 16/42 (38%), Positives = 21/42 (50%) Frame = +2 Query: 389 EAREDLAALEKDYEEVGVDSTEGELDEENEY*SGMKNSRSVL 514 E DL A D E ++ + ELDEEN Y ++ S S L Sbjct: 72 EEDPDLEAYLSDLEREELEHSLEELDEENNYKLHLRYSFSQL 113 >SPAC22E12.11c |set3||histone lysine methyltransferase Set3|Schizosaccharomyces pombe|chr 1|||Manual Length = 859 Score = 25.0 bits (52), Expect = 7.9 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = +2 Query: 452 EGELDEENEY*SGMKNSRSVLGRKEPNSY 538 +G + +NEY S KN ++LG +P+ + Sbjct: 245 KGRVCTQNEYKSDPKNQYNILGAPKPHVF 273 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,339,666 Number of Sequences: 5004 Number of extensions: 47236 Number of successful extensions: 156 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 155 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 244081442 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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