Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0428
(722 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 266 3e-72
SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu... 120 3e-28
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ... 109 4e-25
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 101 1e-22
SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit... 91 1e-19
SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ... 86 4e-18
SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit... 84 2e-17
SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C... 81 2e-16
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 32 0.072
SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex ... 28 1.2
SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomy... 28 1.6
SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4 |S... 27 2.1
SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr 2|... 25 8.3
SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa... 25 8.3
SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 25 8.3
>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
Cct6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 266 bits (651), Expect = 3e-72
Identities = 127/221 (57%), Positives = 170/221 (76%)
Frame = +1
Query: 58 ISLLNPKAEFARAAQALAVNISAAKGIQDVMKTNLGPKGTMKMLVSGAGDIKITKDGNVL 237
+SLLNPKAE + AQAL VNISAA G+QDV+K+NLGP GT KMLV GAG IK+TKDG VL
Sbjct: 2 LSLLNPKAESIQRAQALQVNISAAIGLQDVLKSNLGPTGTTKMLVDGAGAIKLTKDGKVL 61
Query: 238 LHEMQIQHPTASLIARXXXXXXXXXXXXXXXXVLLIGELLKQADIFISEGLHPRIITEGF 417
L EMQIQ+PTAS IA+ LL+GELLKQA+++I EGLHP +I++GF
Sbjct: 62 LTEMQIQNPTASCIAKAATAQDDATGDGTTSVCLLVGELLKQAELYIREGLHPSLISDGF 121
Query: 418 DIARNKSLEVLESMKIPIEIARENLVDVARTSLKTKVHPSLADVLTDACVDAVLTIRTPG 597
++A+N++L L+S K E+ RE L++VA+TSL TK+ + + L A VDA+LTIR P
Sbjct: 122 NLAKNEALTFLDSFKTDFEVDREVLLNVAKTSLSTKISSKVVESLAPAVVDAILTIRRPD 181
Query: 598 KPVDLHMVEIMEMKHKTATETVLVKGLVMDHGARHPDMPKR 720
+P+DLHMVEIM+M++++A++T L++GL++DHGARHPDMPK+
Sbjct: 182 EPIDLHMVEIMKMQNRSASDTQLIRGLLLDHGARHPDMPKQ 222
>SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit
Cct5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 546
Score = 120 bits (288), Expect = 3e-28
Identities = 66/210 (31%), Positives = 118/210 (56%), Gaps = 4/210 (1%)
Frame = +1
Query: 103 ALAVNISAAKGIQDVMKTNLGPKGTMKMLVSGAGDIKITKDGNVLLHEMQIQHPTASLIA 282
A+ +I A K + ++++T+LGP+G K+L+S G+I +T DG +L +M+++H A L+
Sbjct: 35 AVKSHILATKTVANIVRTSLGPRGLDKILISPDGEITVTNDGATILDQMEVEHQIAKLLV 94
Query: 283 RXXXXXXXXXXXXXXXXVLLIGELLKQADIFISEGLHPRIITEGFDIARNKSLEVLESMK 462
+ V+L G LL+QA+ I +G+HP I +G++ A +++ L+++
Sbjct: 95 QLSKSQDDEIGDGTTGVVVLAGALLEQAEALIDKGIHPIRIADGYEKACQVAVKHLDAIS 154
Query: 463 IPIEIARENLVDV---ARTSLKTKVHPSLADVLTDACVDAVLTIR-TPGKPVDLHMVEIM 630
++ + EN ++ A+TSL +KV D + VDAVL++ K VD ++++
Sbjct: 155 DVVDFSPENTTNLFRSAKTSLGSKVVSKAHDHFANIAVDAVLSVADLQRKDVDFELIKVD 214
Query: 631 EMKHKTATETVLVKGLVMDHGARHPDMPKR 720
+ +T LVKG+V+D HP MP R
Sbjct: 215 GKVGGSVDDTKLVKGVVVDKDMSHPQMPHR 244
>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
Cct4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 527
Score = 109 bits (262), Expect = 4e-25
Identities = 60/197 (30%), Positives = 104/197 (52%), Gaps = 3/197 (1%)
Frame = +1
Query: 115 NISAAKGIQDVMKTNLGPKGTMKMLVSGAGDIKITKDGNVLLHEMQIQHPTASLIARXXX 294
NI AA+ + D ++T+LGPKG KM+ +G G++ +T DG +L + + HP A ++
Sbjct: 24 NIMAARSVADAIRTSLGPKGMDKMIQTGKGEVILTNDGATILKHLSVLHPAAKMLVDLSA 83
Query: 295 XXXXXXXXXXXXXVLLIGELLKQADIFISEGLHPRIITEGFDIARNKSLEVLESMKIPIE 474
V+L G +L A+ + +G+HP +I E F A +++ ++ + IE
Sbjct: 84 AQDVEAGDGTTSVVILAGSMLACAEKLLKKGIHPTVIAESFQRAAGFTVDCMKENALAIE 143
Query: 475 IA-RENLVDVARTSLKTKVHPSLADVLTDACVDAVLTIRTP--GKPVDLHMVEIMEMKHK 645
++ RE+L+ A TSL +K+ +++L VDAVL + P VDL + I++
Sbjct: 144 LSDRESLLRAATTSLNSKIVSQYSNLLAPIAVDAVLKVIDPRVATNVDLKDIRIVKKLGG 203
Query: 646 TATETVLVKGLVMDHGA 696
+T L+ GL + A
Sbjct: 204 IIDDTELIPGLALTQTA 220
>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
Cct1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 101 bits (241), Expect = 1e-22
Identities = 53/197 (26%), Positives = 109/197 (55%), Gaps = 6/197 (3%)
Frame = +1
Query: 115 NISAAKGIQDVMKTNLGPKGTMKMLVSGAGDIKITKDGNVLLHEMQIQHPTASLIARXXX 294
N+ A I +V+K++LGP G KMLV GD+ +T DG +L + ++HP ++
Sbjct: 26 NVLATTAIANVVKSSLGPVGLDKMLVDDIGDVTVTNDGATILSLLDVEHPAGKVLVELAQ 85
Query: 295 XXXXXXXXXXXXXVLLIGELLKQADIFISEGLHPRIITEGFDIARNKSLEVL-ESMKIPI 471
V++ ELL++A+ + +HP I G+ +A ++++ + + + +
Sbjct: 86 QQDKEVGDGTTSVVIIAAELLRRANELVKNKIHPTTIITGYRLAIREAVKFMTDVLSCSV 145
Query: 472 E-IARENLVDVARTSLKTKVHPSLADVLTDACVDAVLTIRTPGKPVD----LHMVEIMEM 636
+ + +E+L++VA+TS+ +K+ + +D + VDA+L+++T + + V I++
Sbjct: 146 DSLGKESLINVAKTSMSSKIIGNDSDFFSTMAVDAMLSVKTSNSKGETRYPVKAVNILKA 205
Query: 637 KHKTATETVLVKGLVMD 687
K++ E+VLVKG ++
Sbjct: 206 HGKSSRESVLVKGYALN 222
>SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit
Cct3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 528
Score = 91.1 bits (216), Expect = 1e-19
Identities = 48/207 (23%), Positives = 100/207 (48%), Gaps = 5/207 (2%)
Frame = +1
Query: 115 NISAAKGIQDVMKTNLGPKGTMKMLVSGAGDIKITKDGNVLLHEMQIQHPTASLIARXXX 294
NI AAK + DV++T LGP+ +KML+ G + +T DG+ +L E+++ HP A +
Sbjct: 24 NIQAAKAVADVIRTCLGPRAMLKMLLDPVGSVLLTNDGHAILREIEVAHPAAKSMIELAR 83
Query: 295 XXXXXXXXXXXXXVLLIGELLKQADIFISEGLHPRIITEGFDIARNKSLEVLESMKIPIE 474
++L GE+L A + +HP ++ F A +L +++ + +P+
Sbjct: 84 TQDEEVGDGTTSVIILAGEILAAASPLLDRKIHPVVMIRSFKQALEDALSIIDEITLPVN 143
Query: 475 I-ARENLVDVARTSLKTKVHPSLADVLTDACVDAVLTIRTPGK---PVDL-HMVEIMEMK 639
+ + + RT + TK+ +D++ + AV T+ + +D+ + ++
Sbjct: 144 VDDNAEMFRLIRTCIGTKLVARWSDLMCHLALRAVRTVASTSNGRMEIDIKRYARVEKVP 203
Query: 640 HKTATETVLVKGLVMDHGARHPDMPKR 720
+ ++ G++++ HP M +R
Sbjct: 204 GGEIESSCVLDGVMLNKDVTHPKMRRR 230
>SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit
Cct7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 558
Score = 86.2 bits (204), Expect = 4e-18
Identities = 52/196 (26%), Positives = 100/196 (51%), Gaps = 5/196 (2%)
Frame = +1
Query: 106 LAVNISAAKGIQDVMKTNLGPKGTMKMLVSGAGDIKITKDGNVLLHEMQIQHPTASLIAR 285
L NI+A +QD ++T LGP G K++V G++ I+ DG ++ + I HP A +
Sbjct: 26 LLSNINACVAVQDTIRTTLGPLGADKLMVDDRGEVVISNDGATIMKLLDIVHPAAKTLVD 85
Query: 286 XXXXXXXXXXXXXXXXVLLIGELLKQADIFISEGLHPRIITEGFDIARNKSLEVLESMKI 465
V+ GELL++A F+ +G+ +I G+ A ++ ++ + I
Sbjct: 86 IARAQDAEVGDGTTSVVVFAGELLREARTFVEDGVSSHLIIRGYRKAAQLAVNKIKEIAI 145
Query: 466 PIEIA-----RENLVDVARTSLKTKVHPSLADVLTDACVDAVLTIRTPGKPVDLHMVEIM 630
++++ R+ L A T++ +K+ S + T VDAVLT+ + ++ +M+ I
Sbjct: 146 HLDLSDEGKLRDLLTKCASTAMNSKLIRSNSTFFTKMVVDAVLTL--DQEDLNENMIGIK 203
Query: 631 EMKHKTATETVLVKGL 678
++ +++LVKG+
Sbjct: 204 KVPGGAMEDSLLVKGV 219
>SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit
Cct8 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 546
Score = 84.2 bits (199), Expect = 2e-17
Identities = 52/198 (26%), Positives = 103/198 (52%), Gaps = 5/198 (2%)
Frame = +1
Query: 103 ALAVNISAAKGIQDVMKTNLGPKGTMKMLVSGAGDIKITKDGNVLLHEMQIQHPTASLIA 282
A+ N +A + + ++ +T+LGP G K++V+ +T D ++ E+++ HP A L+
Sbjct: 27 AVIRNCNAIRELSEITRTSLGPNGKNKIVVNHLQQTFLTNDAATIIRELEVIHPAAKLVV 86
Query: 283 RXXXXXXXXXXXXXXXXVLLIGELLKQADIFISEGLHPRIITEGFDIARNKSLEVLESM- 459
V+ GELL +A+ I GL P I +G+++A + ++EVLE +
Sbjct: 87 DATQQQENELGDAANFVVVFTGELLAKAENMIRMGLTPLEIAKGYEMALSHTMEVLEEIC 146
Query: 460 --KIPIEIARENLVDVARTSLKTKVHPSLADVLTDACVDAVLTI--RTPGKPVDLHMVEI 627
KI + + L+ RT + +K + + D L+D A+LT+ + P K ++ + +
Sbjct: 147 ADKIETVESEKELIKAIRTCISSKQYGN-EDFLSDLVAKAILTVLPKDPSK-FNVDNIRV 204
Query: 628 MEMKHKTATETVLVKGLV 681
+++ + + +VKG+V
Sbjct: 205 VKIMGSSLYNSQVVKGMV 222
>SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit
Cct2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 80.6 bits (190), Expect = 2e-16
Identities = 49/186 (26%), Positives = 93/186 (50%), Gaps = 6/186 (3%)
Frame = +1
Query: 91 RAAQALAVNISAAKGIQDVMKTNLGPKGTMKMLVSGA-GDIKITKDGNVLLHEMQIQHPT 267
R A + A + D++K+ LGPKG K+L S + GDI +T DG +L + + +
Sbjct: 17 RGENARLSSFVGAIAVGDLVKSTLGPKGMDKILQSNSSGDIVVTNDGATILKSIALDNAA 76
Query: 268 ASLIARXXXXXXXXXXXXXXXXVLLIGELLKQADIFISEGLHPRIITEGFDIARNKSLEV 447
A ++ + ELL+QA+I ++ +HP++I +G+ IA +++
Sbjct: 77 AKVLVNISKVQDDEVGDGTTSVCVFAAELLRQAEIMVNAKIHPQVIIDGYRIATKTAIDA 136
Query: 448 LESMKI-----PIEIARENLVDVARTSLKTKVHPSLADVLTDACVDAVLTIRTPGKPVDL 612
L + I P + R +L ++ART+L +K+ + VDAVL ++ ++
Sbjct: 137 LRASSIDNSSDPAKF-RSDLENIARTTLSSKILSQNKNHFAQLAVDAVLRLKGSTNLDNI 195
Query: 613 HMVEIM 630
+++I+
Sbjct: 196 QIIKIL 201
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 32.3 bits (70), Expect = 0.072
Identities = 33/153 (21%), Positives = 61/153 (39%), Gaps = 6/153 (3%)
Frame = +1
Query: 148 MKTNLGPKGTMKMLVSGAGDIKITKDGNVLLHEMQIQHPTASLIAR----XXXXXXXXXX 315
+ LGPKG ++ G KITKDG + + ++ +L AR
Sbjct: 58 VSVTLGPKGRNVLIDQPFGSPKITKDGVTVARSVSLKDKFENLGARLVQDVASKTNEVAG 117
Query: 316 XXXXXXVLLIGELLKQADIFISEGLHPRIITEGFDIARNKSLEVLESMKIPIEIARENLV 495
+L + + ++ G +P + G +A + +E L++ K I + E +
Sbjct: 118 DGTTTATVLTRAIFSETVRNVAAGCNPMDLRRGIQLAVDNVVEFLQANKRDITTS-EEIS 176
Query: 496 DVARTSLK--TKVHPSLADVLTDACVDAVLTIR 588
VA S T + LA + + V+T++
Sbjct: 177 QVATISANGDTHIGELLAKAMERVGKEGVITVK 209
>SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex
subunit |Schizosaccharomyces pombe|chr 2|||Manual
Length = 509
Score = 28.3 bits (60), Expect = 1.2
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = +1
Query: 370 IFISEGLHPRIITEGFDIARNKSLEVLESMKI 465
+F+ E + I EGF IAR K +E LE +K+
Sbjct: 437 VFLLEEIVELCIAEGFLIARAKRVESLERVKV 468
>SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 537
Score = 27.9 bits (59), Expect = 1.6
Identities = 18/59 (30%), Positives = 30/59 (50%)
Frame = +1
Query: 469 IEIARENLVDVARTSLKTKVHPSLADVLTDACVDAVLTIRTPGKPVDLHMVEIMEMKHK 645
I I L D++ T K VHPS++ L A + L +++ G + ++VE +HK
Sbjct: 7 ILITPSPLSDISTTINKPNVHPSMS--LLKALPECRLVVKSKGDKNNANVVEFPLDRHK 63
>SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 690
Score = 27.5 bits (58), Expect = 2.1
Identities = 12/23 (52%), Positives = 13/23 (56%)
Frame = -2
Query: 574 PHQRKHQLAHLPSLGGP*F*VKY 506
PHQR H A L GGP F + Y
Sbjct: 63 PHQRAHNAAALARSGGPGFSMNY 85
>SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 997
Score = 25.4 bits (53), Expect = 8.3
Identities = 8/13 (61%), Positives = 9/13 (69%)
Frame = +3
Query: 660 CTCKRSSNGSWCT 698
C +R SNG WCT
Sbjct: 973 CIIERDSNGRWCT 985
>SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1072
Score = 25.4 bits (53), Expect = 8.3
Identities = 11/43 (25%), Positives = 27/43 (62%)
Frame = +1
Query: 430 NKSLEVLESMKIPIEIARENLVDVARTSLKTKVHPSLADVLTD 558
++SL++L+ + ++ ++ VD+ART ++ S ++V+ D
Sbjct: 270 SESLDLLDDFNYQVTVSNKSFVDIARTEYFITIY-SDSNVILD 311
>SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 764
Score = 25.4 bits (53), Expect = 8.3
Identities = 9/32 (28%), Positives = 17/32 (53%)
Frame = -3
Query: 219 GDLYVPSTRNQHFHRAFRTKVSFHYILNSFSC 124
G + S RN+ FHR F+ ++++ + C
Sbjct: 187 GYAFANSKRNRDFHRIFKVLPPEDHLIDDYGC 218
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,860,323
Number of Sequences: 5004
Number of extensions: 54988
Number of successful extensions: 145
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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