BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0423 (710 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC364.06 |nap1||nucleosome assembly protein Nap1 |Schizosaccha... 99 7e-22 SPBC2D10.11c |||nucleosome assembly protein Nap2 |Schizosaccharo... 77 2e-15 SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa... 27 2.6 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 26 4.6 SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 26 6.1 SPAC13G6.03 |gpi7||GPI anchor biosynthesis protein Gpi7 |Schizos... 26 6.1 SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 8.1 >SPCC364.06 |nap1||nucleosome assembly protein Nap1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 393 Score = 98.7 bits (235), Expect = 7e-22 Identities = 63/180 (35%), Positives = 88/180 (48%) Frame = +3 Query: 147 LHAEAMASLPPNVRRRIRALRTLQKEFVDIEAKFYSEVHAXXXXXXXXXXXXXXXRALIV 326 L +E ++ LP V+RRI LR LQK + D+E++F E+ R+ +V Sbjct: 63 LTSEGVSELPEAVQRRISGLRGLQKRYSDLESQFQKELFELEKAYAKKYAPIFKRRSEVV 122 Query: 327 NGTYEPNDDECLNPWRDDTEEEELARAVQNAAITEGEEKKDDKAIEPPMDPNVKGIPDFW 506 G EP ++E ++ E A + T E KK + + KGIP+FW Sbjct: 123 RGADEPTEEEI--------KKGEAADENEKKEPTSSESKKQEGG------DDTKGIPEFW 168 Query: 507 YNIFRNVSMLSEMMQEHDEPILKCLQDIKVQMHEDPISFTLEFYFAPNEYFTNTVLTKEY 686 +NV LSEM+ DE L L DI++ E P F LEF FA N +FTN +LTK Y Sbjct: 169 LTAMKNVLSLSEMITPEDEGALSHLVDIRISYMEKP-GFKLEFEFAENPFFTNKILTKTY 227 >SPBC2D10.11c |||nucleosome assembly protein Nap2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 77.4 bits (182), Expect = 2e-15 Identities = 68/207 (32%), Positives = 93/207 (44%), Gaps = 9/207 (4%) Frame = +3 Query: 102 SGVTRNE--MIAAITNRLHAEA------MASLPPNVRRRIRALRTLQKEFVDIEAKFYSE 257 SG+ RN M++ I RL + + SL P V+ RI AL+ LQK+ I+ +F + Sbjct: 56 SGLLRNNPAMLSMIEGRLSSLVGKSSGYIESLAPAVQNRITALKGLQKDCDAIQYEFRQK 115 Query: 258 VHAXXXXXXXXXXXXXXXRALIVNGTYEPNDDECLNPWRDDTEEEELARAVQNAAITEGE 437 + RA I+ G EP DDE D EEE QN Sbjct: 116 MLDLETKYEKKYQPIFSRRAEIIKGVSEPVDDEL------DHEEE----IFQNNL----- 160 Query: 438 EKKDDKAIEPPMDPNVKGIPDFWYNIFRNVSMLSEMMQEHDEPILKCLQDIK-VQMHEDP 614 P+ KGIP+FW NV ++ EM+ DE +L+ L DI+ + D Sbjct: 161 -------------PDPKGIPEFWLTCLHNVFLVGEMITPEDENVLRSLSDIRFTNLSGDV 207 Query: 615 ISFTLEFYFAPNEYFTNTVLTKEYLMK 695 + LEF F N+YFTN +LTK Y K Sbjct: 208 HGYKLEFEFDSNDYFTNKILTKTYYYK 234 >SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosaccharomyces pombe|chr 1|||Manual Length = 783 Score = 27.1 bits (57), Expect = 2.6 Identities = 18/36 (50%), Positives = 20/36 (55%), Gaps = 4/36 (11%) Frame = -2 Query: 670 TVFV---KYSFGAK*-NSKVKLMGSSCICTLISCKH 575 T+FV KYSF +K SK K SC LISC H Sbjct: 354 TLFVSLRKYSFSSKNVQSKEKSKIMSCFTLLISCAH 389 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 26.2 bits (55), Expect = 4.6 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = +3 Query: 411 QNAAITEGEEKKDDKAIEPPMDPNVKGIP 497 +N+ + E EEK D +AIE + +V G P Sbjct: 554 KNSLVNEAEEKNDLEAIEAAKNFHVNGKP 582 >SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2100 Score = 25.8 bits (54), Expect = 6.1 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +3 Query: 90 HLLKSGVTRNEMIAAITNRLHAEAMASLP 176 HLL++ T +E AA +LH + + S P Sbjct: 1596 HLLRNSATNDETKAAFVYQLHKQGILSEP 1624 >SPAC13G6.03 |gpi7||GPI anchor biosynthesis protein Gpi7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 758 Score = 25.8 bits (54), Expect = 6.1 Identities = 16/55 (29%), Positives = 32/55 (58%), Gaps = 4/55 (7%) Frame = -1 Query: 485 YIGIH-WRLNSLVILLFLTLSDGSI---LYRPS*LFFFSVITPWVETFIIIRFIC 333 Y G+ + + ++ ILLF ++ G++ L++P + SV T W+ + I + F+C Sbjct: 659 YTGLDSYNIFAVGILLFTSVFAGALWWCLHQPKRMMDRSVKTFWIMSSISLTFLC 713 >SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1038 Score = 25.4 bits (53), Expect = 8.1 Identities = 10/36 (27%), Positives = 21/36 (58%) Frame = +3 Query: 387 EEELARAVQNAAITEGEEKKDDKAIEPPMDPNVKGI 494 EEEL+ ++ N + E++KD+ +P P++ + Sbjct: 475 EEELSDSLSNDFGIDAEKEKDENLSKPEHHPSITSV 510 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,883,750 Number of Sequences: 5004 Number of extensions: 58394 Number of successful extensions: 210 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 200 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 207 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 331187010 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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