Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0420
         (645 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ667191-1|ABG75743.1|  475|Apis mellifera pH-sensitive chloride...    27   0.16 
DQ026038-1|AAY87897.1|  520|Apis mellifera nicotinic acetylcholi...    24   1.1  
DQ667187-1|ABG75739.1|  428|Apis mellifera histamine-gated chlor...    23   1.9  
AF069739-1|AAC63272.2|  690|Apis mellifera translation initiatio...    23   2.5  

>DQ667191-1|ABG75743.1|  475|Apis mellifera pH-sensitive chloride
           channel variant 3 protein.
          Length = 475

 Score = 27.1 bits (57), Expect = 0.16
 Identities = 16/46 (34%), Positives = 23/46 (50%)
 Frame = +3

Query: 126 HTLRYSSKVSV*SQRLPHPSNRNALLLHGRNRRSGGTYPCGLTRGP 263
           H + Y    +  +QRLP   +R  ++L    +R GG  P G T GP
Sbjct: 363 HNVVYRPGENPVTQRLPAVLSRIGIILASPLKREGGP-PTGATTGP 407


>DQ026038-1|AAY87897.1|  520|Apis mellifera nicotinic acetylcholine
           receptor beta1subunit protein.
          Length = 520

 Score = 24.2 bits (50), Expect = 1.1
 Identities = 10/30 (33%), Positives = 16/30 (53%)
 Frame = +3

Query: 69  PPDVKWLLEPIDIYNANAPHTLRYSSKVSV 158
           PPD  W  + +   NA+  + +RY S V +
Sbjct: 104 PPDKVWKPDIVLFNNADGNYEVRYKSNVLI 133


>DQ667187-1|ABG75739.1|  428|Apis mellifera histamine-gated chloride
           channel protein.
          Length = 428

 Score = 23.4 bits (48), Expect = 1.9
 Identities = 10/34 (29%), Positives = 17/34 (50%)
 Frame = +3

Query: 528 HYFFIYYDSKFAKLRRCYKKVIYSFNFVIL*HTT 629
           HY ++Y D     + +   K+  + NF+I  H T
Sbjct: 141 HYLWLYKDKTILYMVKLTLKLSCAMNFLIYPHDT 174



 Score = 21.4 bits (43), Expect = 7.7
 Identities = 13/47 (27%), Positives = 22/47 (46%)
 Frame = +3

Query: 183 SNRNALLLHGRNRRSGGTYPCGLTRGPTTSNYANYNFAGFTFITRCY 323
           +  NA+LL GR+++S      G   GPT +  A         ++R +
Sbjct: 366 TKENAMLLSGRSQKS----TTGPPPGPTPAQKARMRALNIDRVSRVF 408


>AF069739-1|AAC63272.2|  690|Apis mellifera translation initiation
           factor 2 protein.
          Length = 690

 Score = 23.0 bits (47), Expect = 2.5
 Identities = 9/32 (28%), Positives = 15/32 (46%)
 Frame = +3

Query: 510 CNQSNLHYFFIYYDSKFAKLRRCYKKVIYSFN 605
           C  + +HY   +      +L   +K +IY FN
Sbjct: 507 CQLNIVHYGIGFITQSDIELANTFKAIIYGFN 538


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 186,107
Number of Sequences: 438
Number of extensions: 4239
Number of successful extensions: 10
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 19438227
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)