Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0417
(657 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g42050.1 68416.m04311 vacuolar ATP synthase subunit H family ... 63 2e-10
At4g31490.1 68417.m04473 coatomer beta subunit, putative / beta-... 37 0.010
At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-... 37 0.010
At5g16780.1 68418.m01965 SART-1 family protein contains Pfam dom... 33 0.13
At1g31730.1 68414.m03893 epsilon-adaptin, putative similar to SP... 33 0.13
At2g17930.1 68415.m02076 FAT domain-containing protein / phospha... 30 1.2
At5g42600.1 68418.m05186 pentacyclic triterpene synthase, putati... 28 6.3
At5g02100.1 68418.m00131 oxysterol-binding family protein simila... 28 6.3
At1g78500.1 68414.m09150 pentacyclic triterpene synthase, putati... 28 6.3
At5g38350.1 68418.m04628 disease resistance protein (NBS-LRR cla... 27 8.3
At1g66730.1 68414.m07585 ATP dependent DNA ligase family protein... 27 8.3
At1g49160.2 68414.m05512 protein kinase family protein contains ... 27 8.3
At1g49160.1 68414.m05511 protein kinase family protein contains ... 27 8.3
>At3g42050.1 68416.m04311 vacuolar ATP synthase subunit H family
protein identical to probable vacuolar ATP synthase
subunit H (EC 3.6.3.14)(V-ATPase H subunit) (Vacuolar
proton pump H subunit) (Vacuolar proton pump subunit
SFD) SP:Q9LX65 from [Arabidopsis thaliana]; contains
Pfam PF03224: V-ATPase subunit H
Length = 441
Score = 62.9 bits (146), Expect = 2e-10
Identities = 29/133 (21%), Positives = 76/133 (57%), Gaps = 4/133 (3%)
Frame = +2
Query: 182 QIRASEIRQTQINWQSYLQSQMITQRDHDFIVNLYQRGQK---DLPDKNPDACAEVFLNL 352
++ ++ + I W++Y+ +++++ + + ++ + L D++ A +F+++
Sbjct: 5 ELSIEQVLKRDIPWETYMNTKLVSAKGLQLLRRYDKKPESARAQLLDEDGPAYVHLFVSI 64
Query: 353 LTHISKDHTIQYILVLIDDILSEDKSRVKIFRETKFSG-NVWQPFLNLLNRQDEFVQHMT 529
L I K+ T++Y+L LI ++LS + +R ++F + + + ++PFL LL + + F+Q +
Sbjct: 65 LRDIFKEETVEYVLALIYEMLSANPTRARLFHDESLANEDTYEPFLRLLWKGNWFIQEKS 124
Query: 530 ARIIAKLACWHPQ 568
+I+A + P+
Sbjct: 125 CKILAWIISARPK 137
>At4g31490.1 68417.m04473 coatomer beta subunit, putative /
beta-coat protein, putative / beta-COP, putative similar
to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
from {Rattus norvegicus} SP|P23514, {Mus musculus}
SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 948
Score = 37.1 bits (82), Expect = 0.010
Identities = 19/80 (23%), Positives = 45/80 (56%), Gaps = 2/80 (2%)
Frame = +2
Query: 317 NPDACAEVFLNLLTHI--SKDHTIQYILVLIDDILSEDKSRVKIFRETKFSGNVWQPFLN 490
N + ++F+ ++ ++ S+DHTIQ +L+L +++ + S+ K+ E + Q N
Sbjct: 47 NGETIPQLFITIIRYVLPSEDHTIQKLLLLYLELIEKTDSKGKVLPEMIL---ICQNLRN 103
Query: 491 LLNRQDEFVQHMTARIIAKL 550
L +E+++ +T R + ++
Sbjct: 104 NLQHPNEYIRGVTLRFLCRM 123
>At4g31480.1 68417.m04472 coatomer beta subunit, putative /
beta-coat protein, putative / beta-COP, putative similar
to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
from {Rattus norvegicus} SP|P23514, {Mus musculus}
SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 971
Score = 37.1 bits (82), Expect = 0.010
Identities = 19/80 (23%), Positives = 45/80 (56%), Gaps = 2/80 (2%)
Frame = +2
Query: 317 NPDACAEVFLNLLTHI--SKDHTIQYILVLIDDILSEDKSRVKIFRETKFSGNVWQPFLN 490
N + ++F+ ++ ++ S+DHTIQ +L+L +++ + S+ K+ E + Q N
Sbjct: 70 NGETIPQLFITIIRYVLPSEDHTIQKLLLLYLELIEKTDSKGKVLPEMIL---ICQNLRN 126
Query: 491 LLNRQDEFVQHMTARIIAKL 550
L +E+++ +T R + ++
Sbjct: 127 NLQHPNEYIRGVTLRFLCRM 146
>At5g16780.1 68418.m01965 SART-1 family protein contains Pfam
domain, PF03343: SART-1 family
Length = 820
Score = 33.5 bits (73), Expect = 0.13
Identities = 16/38 (42%), Positives = 21/38 (55%)
Frame = +1
Query: 94 ANVSEENVSQLIPTLGDDKNRHDCGHQRPTDPGK*DTP 207
A V ++ L P LGD K H G +R ++PG DTP
Sbjct: 777 ATVEKDVPGSLTPMLGDRKVEHFLGIKRKSEPGNSDTP 814
>At1g31730.1 68414.m03893 epsilon-adaptin, putative similar to
SP|Q9UPM8 Adapter-related protein complex 4 epsilon 1
subunit (Epsilon subunit of AP-4) (AP-4 adapter complex
epsilon subunit) {Homo sapiens}; contains Pfam profile:
PF01602 Adaptin N terminal region
Length = 938
Score = 33.5 bits (73), Expect = 0.13
Identities = 21/71 (29%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
Frame = +2
Query: 230 YLQSQMITQRDHDFIVNLYQRGQKDL-PDKNPDACAEVFLNLLTHISKDHTIQYILVLID 406
YL + DHD I+ + QKDL D CA LN + + + TI +L +
Sbjct: 106 YLAVTLFLNEDHDLIILIVNTIQKDLRSDNYLVVCAA--LNAICRLINEETIPAVLPQVV 163
Query: 407 DILSEDKSRVK 439
++L+ K V+
Sbjct: 164 ELLNHQKEAVR 174
>At2g17930.1 68415.m02076 FAT domain-containing protein /
phosphatidylinositol 3- and 4-kinase family protein
contains Pfam profiles PF02259 FAT domain, PF00454
Phosphatidylinositol 3- and 4-kinase, PF02260: FATC
domain
Length = 3795
Score = 30.3 bits (65), Expect = 1.2
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = -3
Query: 415 KNVINEHKNVLNGVVLADMRQEVEKDFGTGIGILVRQ 305
K + N HKN ++ VL + Q + +D G+ +G RQ
Sbjct: 2192 KTLANVHKNFVDSYVLVRILQRLARDLGSAVGSHPRQ 2228
>At5g42600.1 68418.m05186 pentacyclic triterpene synthase, putative
similar to pentacyclic triterpene synthase [gi:6650207]
[PMID: 11247608]
Length = 761
Score = 27.9 bits (59), Expect = 6.3
Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 4/35 (11%)
Frame = +2
Query: 485 LNLLNRQDEFVQHMTARIIAK----LACWHPQPDG 577
+ L++ DE ++T +AK LACW PDG
Sbjct: 355 MELVHYHDETTHYITGACVAKPFHMLACWVEDPDG 389
>At5g02100.1 68418.m00131 oxysterol-binding family protein similar
to SWH1 [Saccharomyces cerevisiae] GI:402658; contains
Pfam profile PF01237: Oxysterol-binding protein
Length = 453
Score = 27.9 bits (59), Expect = 6.3
Identities = 11/31 (35%), Positives = 14/31 (45%)
Frame = +2
Query: 473 WQPFLNLLNRQDEFVQHMTARIIAKLACWHP 565
W+PF +L E H IA+ C HP
Sbjct: 142 WKPFNPILGETYEMTNHNGINFIAEQVCHHP 172
>At1g78500.1 68414.m09150 pentacyclic triterpene synthase, putative
similar to pentacyclic triterpene synthase (04C11)
[gi:6650208] [PMID:11247608]; similar to beta-Amyrin
Synthase GI:3688600 from [Panax ginseng]
Length = 767
Score = 27.9 bits (59), Expect = 6.3
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 4/35 (11%)
Frame = +2
Query: 485 LNLLNRQDEFVQHMTARIIAK----LACWHPQPDG 577
+ LL+ QDE ++ T + K LACW PDG
Sbjct: 356 MELLHYQDEASRYFTGGCVPKPFHMLACWVEDPDG 390
>At5g38350.1 68418.m04628 disease resistance protein (NBS-LRR
class), putative domain signature NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 833
Score = 27.5 bits (58), Expect = 8.3
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +1
Query: 97 NVSEENVSQLIPTLGDDKNRHDCGHQRPTDPGK 195
N + + L+ LG D RH GHQ +PGK
Sbjct: 324 NCTRIEMHNLLVQLGKDIVRHKPGHQSICEPGK 356
>At1g66730.1 68414.m07585 ATP dependent DNA ligase family protein
contains Pfam profile: PF01068 ATP dependent DNA ligase
domain
Length = 1417
Score = 27.5 bits (58), Expect = 8.3
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = -2
Query: 536 CEQSCAGQIHPDGSANSGRAAKRCQRISSHE 444
C ++C+ Q S NSG A C + S+ E
Sbjct: 707 CSEACSSQEDKKASENSGHAVNICVQTSAEE 737
>At1g49160.2 68414.m05512 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 557
Score = 27.5 bits (58), Expect = 8.3
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +3
Query: 561 IRSLMDKSDLHFYLSWLKDQLKTNN 635
++SL K+ + FY SW+ D+ KT N
Sbjct: 81 LKSLKHKNIIRFYNSWIDDKNKTVN 105
>At1g49160.1 68414.m05511 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 539
Score = 27.5 bits (58), Expect = 8.3
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +3
Query: 561 IRSLMDKSDLHFYLSWLKDQLKTNN 635
++SL K+ + FY SW+ D+ KT N
Sbjct: 63 LKSLKHKNIIRFYNSWIDDKNKTVN 87
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,818,990
Number of Sequences: 28952
Number of extensions: 267008
Number of successful extensions: 724
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 707
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 724
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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