Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0409
(722 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 110 2e-25
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 45 1e-05
SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizo... 29 0.67
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 27 3.6
SPAC23G3.05c |||regulator of G-protein signaling |Schizosaccharo... 26 4.7
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 4.7
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 26 4.7
SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 4.7
SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces p... 26 6.3
SPBC1604.01 |mug158|SPBC1677.01c|sulfatase modifying factor 1 re... 26 6.3
SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosa... 26 6.3
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu... 26 6.3
SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch... 26 6.3
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 25 8.3
SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ... 25 8.3
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 110 bits (265), Expect = 2e-25
Identities = 66/167 (39%), Positives = 91/167 (54%), Gaps = 10/167 (5%)
Frame = +1
Query: 151 DKGLKTSEDARFYALSRKF-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKD 327
+ GL ++A +A+S +F +P + K LVVQ+ V E+ ++CGG YLK+ + +
Sbjct: 89 EAGLVMKDEAAHHAISYEFDEPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTHGE 147
Query: 328 MHGETPYEIMFGPDICGPGTKKVHVIFSYKG-------KNHLIKKDIRCKDDVYTHLYTL 486
M Y IMFGPD CG + VH IF +K + HL + T+LYTL
Sbjct: 148 MSNSIDYRIMFGPDKCGVNDR-VHFIFKHKNPLTGEYSEKHLDSRPASLLKPGITNLYTL 206
Query: 487 IVKPDNTYEVLIDNEKVESGDLEADW--DFLPPKKIKDPEAKKPEDW 621
IVKPD T+EV I+ + V G L D+ LPP +I DPE KP DW
Sbjct: 207 IVKPDQTFEVRINGDVVRQGSLFYDFIPPVLPPVEIYDPEDIKPADW 253
Score = 35.1 bits (77), Expect = 0.010
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = +1
Query: 559 DWDFLPPKKIKDPEAKKPEDW 621
DWD P+ I DP+A KPEDW
Sbjct: 269 DWDEDAPRMIPDPDAVKPEDW 289
Score = 31.5 bits (68), Expect = 0.13
Identities = 13/21 (61%), Positives = 14/21 (66%)
Frame = +1
Query: 559 DWDFLPPKKIKDPEAKKPEDW 621
DW P I DPEA+KPEDW
Sbjct: 288 DWLEDEPLYIPDPEAQKPEDW 308
Score = 25.4 bits (53), Expect = 8.3
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = +2
Query: 692 PDPDATKPED 721
PDPDA KPED
Sbjct: 279 PDPDAVKPED 288
>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 44.8 bits (101), Expect = 1e-05
Identities = 52/194 (26%), Positives = 82/194 (42%), Gaps = 8/194 (4%)
Frame = +1
Query: 31 FEEKFPDD---SWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL-S 198
F E+F + +W S W K+ G + L S D+ GL T + + + L S
Sbjct: 33 FSEQFTSEDITTWRSRW--RAPVNKDLGVWDLVEAPG-SHLRDEYGLITLKSNKPHILIS 89
Query: 199 RKFKPFSNEGK--PLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDI 372
P + + P+V+ F VK + CG Y+ + + K++ E P I FG
Sbjct: 90 NLENPTTRQSSSVPIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVKK 148
Query: 373 CGPGTKKVHVIFSYKGK--NHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESG 546
CG I SY GK HL + T +YTL+++ + T + D V +G
Sbjct: 149 CGMFDYISLSIISYDGKVSCHLYDAPPSGLVEGRTSMYTLLLQ-NTTVVIRRDQSVVYTG 207
Query: 547 DLEADWDFLPPKKI 588
D+ ++ P K I
Sbjct: 208 DVGTNFFHSPTKWI 221
>SPBC1773.09c |mug184||meiotically upregulated gene
Mug184|Schizosaccharomyces pombe|chr 2|||Manual
Length = 551
Score = 29.1 bits (62), Expect = 0.67
Identities = 19/71 (26%), Positives = 34/71 (47%), Gaps = 1/71 (1%)
Frame = +1
Query: 409 SYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLE-ADWDFLPPKK 585
S + K H KK +D Y+H+Y + P + + + ++ ES E + D+LP
Sbjct: 137 STRKKEHFNKKPSFPRDTEYSHIYNMKYDPRSGIGIRVKRQEPESLKKENNNSDYLPKSA 196
Query: 586 IKDPEAKKPED 618
+K + P+D
Sbjct: 197 MKQKKG-GPKD 206
>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 736
Score = 26.6 bits (56), Expect = 3.6
Identities = 18/71 (25%), Positives = 32/71 (45%), Gaps = 6/71 (8%)
Frame = +1
Query: 280 GGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGTK----KVHVIFSYKGKNHLIKKDI 447
G +LKV + E M ETP +I++ + G+ +H K + KK++
Sbjct: 614 GKKFLKVDEATREDMLMSAETPDDILWITTLISTGSSMSHFPLHAYLEAKERQEFTKKNL 673
Query: 448 R--CKDDVYTH 474
CK+D + +
Sbjct: 674 LLFCKEDEFLY 684
>SPAC23G3.05c |||regulator of G-protein signaling
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 343
Score = 26.2 bits (55), Expect = 4.7
Identities = 17/69 (24%), Positives = 33/69 (47%)
Frame = +1
Query: 292 LKVFDCKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYT 471
+K+F + +K + +P+E+M P + P K H + ++R +DV T
Sbjct: 154 VKMFTQIIIEKYFNPASPHEVMLPPQLVQP---------ILDCKEHQRQDELRLFEDVET 204
Query: 472 HLYTLIVKP 498
+L ++KP
Sbjct: 205 YLLNFLLKP 213
>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
Thi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 26.2 bits (55), Expect = 4.7
Identities = 10/32 (31%), Positives = 18/32 (56%)
Frame = -1
Query: 185 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFP 90
N +E F+P + SL N P +N ++ ++ P
Sbjct: 685 NSTPNEAFQPFDQTSSLYNVPGLNKSYVSNQP 716
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 26.2 bits (55), Expect = 4.7
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = +1
Query: 37 EKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDA 180
+ F +D + +W EH K ++ + + FS+ DD+ + SE +
Sbjct: 49 QAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSE-GDDEPFEVSESS 95
>SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 391
Score = 26.2 bits (55), Expect = 4.7
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +1
Query: 538 ESGDLEADWDFLPPKKIKDPEAKKPEDW 621
E L+ LP + K P AKKPED+
Sbjct: 157 EKNTLDLKHCLLPSPEYKAPPAKKPEDY 184
>SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1040
Score = 25.8 bits (54), Expect = 6.3
Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 4/58 (6%)
Frame = -1
Query: 209 LNLRERA*NLASSEV----FKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESS 48
++LR RA L+ E+ + LS+ LK +++ FP SFP +Y + ++SS
Sbjct: 121 VDLRMRAIFLSIDEISMICIRILSAEERLKTGRSIDSGFPFSFPVHLIYDMCILNDSS 178
>SPBC1604.01 |mug158|SPBC1677.01c|sulfatase modifying factor 1
related|Schizosaccharomyces pombe|chr 2|||Manual
Length = 773
Score = 25.8 bits (54), Expect = 6.3
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +1
Query: 4 LSSINCDVFFEEKFPDDSWESNWVYSEHPG 93
L+S+N VF E F ++ WE VY E G
Sbjct: 227 LASVNA-VFGENLFDENDWEYKSVYDEDLG 255
>SPBC215.15 |sec13||COPII-coated vesicle component
Sec13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 25.8 bits (54), Expect = 6.3
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1
Query: 34 EEKFPDDSWESNWVYS 81
EEKFPD +W +W S
Sbjct: 251 EEKFPDIAWRVSWSLS 266
>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1405
Score = 25.8 bits (54), Expect = 6.3
Identities = 16/65 (24%), Positives = 29/65 (44%)
Frame = +1
Query: 421 KNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWDFLPPKKIKDPE 600
KNH ++ K+ V L + DN Y+ ++ + K + L+ D P +K +
Sbjct: 995 KNHALRIGNEFKEKVNERYSKLEIDIDNVYQRMLLHAKKKYAALQLDSQGKPNLDVKGLD 1054
Query: 601 AKKPE 615
K+ E
Sbjct: 1055 MKRRE 1059
>SPCC63.11 |prp28||U5 snRNP-associated protein Prp28
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 25.8 bits (54), Expect = 6.3
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +1
Query: 559 DWDFLPPKKIKDPEAKKPEDW 621
DWD +P ++ K EA KP DW
Sbjct: 209 DWDDVPWRE-KPLEAMKPRDW 228
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 25.4 bits (53), Expect = 8.3
Identities = 18/58 (31%), Positives = 23/58 (39%)
Frame = +1
Query: 163 KTSEDARFYALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHG 336
KT Y +SRK P L + F K DC G L+V D L+ +G
Sbjct: 952 KTIPRDLLYEVSRKINPLHQASTRLAIAFPDKRLLQYDC--GKLQVLDRLLKDLVSNG 1007
>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1364
Score = 25.4 bits (53), Expect = 8.3
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = +1
Query: 121 AGKFFSDPEDDKGLKTSEDARFYALSRKFKPFSNE 225
A F S E KGL +SE Y+L P SN+
Sbjct: 263 ADSFLSHSESIKGLSSSEQGTVYSLKASHDP-SNQ 296
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,174,243
Number of Sequences: 5004
Number of extensions: 71214
Number of successful extensions: 247
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 228
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 243
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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