Bombyx BLAST Result against nematostella

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0409
         (722 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)             142   3e-34
SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)                94   1e-19
SB_29195| Best HMM Match : No HMM Matches (HMM E-Value=.)              38   0.006
SB_50300| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   0.95 
SB_5548| Best HMM Match : PT (HMM E-Value=3.1)                         31   0.95 
SB_39120| Best HMM Match : SH3_1 (HMM E-Value=2e-20)                   30   2.2  
SB_48987| Best HMM Match : Ank (HMM E-Value=2.7e-36)                   29   2.9  
SB_10791| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   3.8  
SB_27442| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   5.0  
SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   5.0  
SB_5367| Best HMM Match : wnt (HMM E-Value=0)                          29   5.0  
SB_45399| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.7  

>SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1137

 Score =  142 bits (343), Expect = 3e-34
 Identities = 85/200 (42%), Positives = 113/200 (56%), Gaps = 1/200 (0%)
 Frame = +1

Query: 25   VFFEEKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRK 204
            V F EKF D SWE  WV S   G + GKFK TAGKF+ D E DKG++TSEDA+FY +S K
Sbjct: 756  VHFLEKFEDKSWEDRWVSSTSKGAQQGKFKWTAGKFYGDAEADKGIQTSEDAKFYGISAK 815

Query: 205  F-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGP 381
            F KPF+NEGK LV+QF+VKHEQ+IDCGGGY K      + +  H    Y ++  PD    
Sbjct: 816  FEKPFTNEGKTLVIQFSVKHEQNIDCGGGYAK------DDEMTH---LYTLIVRPD---- 862

Query: 382  GTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEAD 561
             T +V +       N  ++     KD  +    T+        E   D + ++  + +  
Sbjct: 863  NTYEVKI------DNEKVESGELEKDWDFLPPKTIKDPEAKKPEDWDDKKMIDDPEDKKP 916

Query: 562  WDFLPPKKIKDPEAKKPEDW 621
             D+  P+ I DP+AKKPEDW
Sbjct: 917  EDWDKPQNIPDPDAKKPEDW 936


>SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)
          Length = 1086

 Score = 93.9 bits (223), Expect = 1e-19
 Identities = 53/117 (45%), Positives = 68/117 (58%), Gaps = 12/117 (10%)
 Frame = +1

Query: 307 CKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKG-KNHLIKKDIRCK--------- 456
           C L Q+    +TPY IMFGPD CG   +K+H IF +K  KN  I++    K         
Sbjct: 28  CHLFQESFGDKTPYTIMFGPDKCGED-RKLHFIFRHKNPKNGTIEEKHAKKPTGNYNSVF 86

Query: 457 DDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADW--DFLPPKKIKDPEAKKPEDW 621
           D   THL+TL+V+PDNT+EV ID E V  G L  D      PP +I+DP+ K+PEDW
Sbjct: 87  DGKKTHLFTLVVRPDNTFEVFIDQESVNKGSLLEDMTPPVNPPAEIEDPDDKRPEDW 143



 Score = 35.1 bits (77), Expect = 0.058
 Identities = 17/35 (48%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
 Frame = +1

Query: 523 DNEKVESGDLEA--DWDFLPPKKIKDPEAKKPEDW 621
           + EK+   D E   DWD   P KI D  AKKPE W
Sbjct: 145 EREKIPDPDAEKPDDWDEDAPVKIPDTNAKKPEGW 179


>SB_29195| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 539

 Score = 38.3 bits (85), Expect = 0.006
 Identities = 29/96 (30%), Positives = 43/96 (44%), Gaps = 3/96 (3%)
 Frame = -1

Query: 284 PPQSMSCSCLTVNWTTKGLPSLLNG---LNLRERA*NLASSEVFKPLSSSGSLKNFPAVN 114
           P +  SC CL V W T GLP L++G   L    R   L+   +  P   S SL +   V 
Sbjct: 429 PSEQNSCQCLFVGWQTVGLPQLIHGTVALISFRRKRTLSGGSL--PRGRSRSLSS-DRVG 485

Query: 113 LNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIED 6
              P++  G +    +    S+G+  S+ +SQ   D
Sbjct: 486 TRLPSARKGSASKIGIPRTSSTGSLGSRRSSQSSTD 521


>SB_50300| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 3669

 Score = 31.1 bits (67), Expect = 0.95
 Identities = 15/40 (37%), Positives = 23/40 (57%)
 Frame = -1

Query: 245  WTTKGLPSLLNGLNLRERA*NLASSEVFKPLSSSGSLKNF 126
            +TT+GLPS ++ +NL E   NL S    KP   S  + ++
Sbjct: 959  YTTQGLPSKISSVNLTEALSNLISISWSKPSDGSSLITDY 998


>SB_5548| Best HMM Match : PT (HMM E-Value=3.1)
          Length = 157

 Score = 31.1 bits (67), Expect = 0.95
 Identities = 25/75 (33%), Positives = 33/75 (44%), Gaps = 1/75 (1%)
 Frame = +2

Query: 500 TTPMKSSLTMRKLNLAT*RQTGTSFRLRKSRTLKPRNQKTGMTSPLFQTPKTRSLRIGTS 679
           T+P  S  TMR  +L T  +T    R +  R   P+   +  TSP   +PKT   + G  
Sbjct: 9   TSPKTSQKTMRIKSLKTTPKTSPKTRRKTGRKTSPKT--SPKTSPK-TSPKTSQKQAGRQ 65

Query: 680 LNTFP-DPDATKPED 721
               P D    KPED
Sbjct: 66  AGNKPEDRTEYKPED 80


>SB_39120| Best HMM Match : SH3_1 (HMM E-Value=2e-20)
          Length = 524

 Score = 29.9 bits (64), Expect = 2.2
 Identities = 20/70 (28%), Positives = 34/70 (48%), Gaps = 1/70 (1%)
 Frame = +2

Query: 497 LTTPMKSSLTMRKLNLAT*RQTGT-SFRLRKSRTLKPRNQKTGMTSPLFQTPKTRSLRIG 673
           L + +++  T R ++    R  G  SF+     T+  +  +    +PLFQTP  ++L+  
Sbjct: 140 LDSVLETPGTARLMDTGMLRTPGNASFQTPGVNTMYQQQIQRTPETPLFQTPPKQTLQAH 199

Query: 674 TSLNTFPDPD 703
           T   TF  PD
Sbjct: 200 TGRVTFQSPD 209


>SB_48987| Best HMM Match : Ank (HMM E-Value=2.7e-36)
          Length = 551

 Score = 29.5 bits (63), Expect = 2.9
 Identities = 16/42 (38%), Positives = 21/42 (50%)
 Frame = +1

Query: 442 DIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWD 567
           D+ CKDD         V  D T E L+D+ +V+S  L  D D
Sbjct: 485 DVSCKDDKNRTALHWAVGQDKTIEGLLDDPRVQSLQLVNDQD 526


>SB_10791| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 510

 Score = 29.1 bits (62), Expect = 3.8
 Identities = 12/31 (38%), Positives = 18/31 (58%)
 Frame = +1

Query: 523 DNEKVESGDLEADWDFLPPKKIKDPEAKKPE 615
           D++  + GD E +  FLPP    + E+K PE
Sbjct: 106 DSDNGDKGDSEDEESFLPPSIFDESESKGPE 136


>SB_27442| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 165

 Score = 28.7 bits (61), Expect = 5.0
 Identities = 13/32 (40%), Positives = 22/32 (68%), Gaps = 1/32 (3%)
 Frame = +1

Query: 529 EKVESGDLEADWD-FLPPKKIKDPEAKKPEDW 621
           EKV +G++  ++D  LPP++ K PE+  PE +
Sbjct: 48  EKVWTGEVWREFDPLLPPRRQKGPESLDPETY 79


>SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 125

 Score = 28.7 bits (61), Expect = 5.0
 Identities = 10/22 (45%), Positives = 17/22 (77%)
 Frame = -3

Query: 87  VLTVHPIAFPRIIRKLLLKEYI 22
           +LT H +  P++IRK+ LK+Y+
Sbjct: 73  LLTAHLVVAPKLIRKMPLKDYV 94


>SB_5367| Best HMM Match : wnt (HMM E-Value=0)
          Length = 367

 Score = 28.7 bits (61), Expect = 5.0
 Identities = 9/33 (27%), Positives = 17/33 (51%)
 Frame = -2

Query: 547 RQIQLSHCQ*GLHRCCQVSQSECTNVCRHHLCS 449
           +++Q + C+   H CC+V    C      H+C+
Sbjct: 335 KEVQATRCRCKFHWCCKVKCKTCIKNVTTHICN 367


>SB_45399| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 573

 Score = 28.3 bits (60), Expect = 6.7
 Identities = 22/97 (22%), Positives = 35/97 (36%), Gaps = 2/97 (2%)
 Frame = -3

Query: 531 LIVNEDFIGVVRFHNQSVQMCVDIIFAADIFFDEMVLTL--VTEDYVYLLGSRTTNVRAE 358
           + + E F   V  HN S Q   DI+   D+      LTL       V  L  + +  +  
Sbjct: 50  IFLGETFASYVSVHNDSNQSVKDIVIKTDLQTSSQRLTLSGAANMPVAKLDPQKSYDQVI 109

Query: 357 HNLIWSLSVHVLLLQFAVKDLEVSASTVNVLFMFDSE 247
           H+ +  L  H+L+   +   L          F F  +
Sbjct: 110 HHEVKELGTHILVCAVSYSSLAGEKMYFRKFFKFQDD 146


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,909,194
Number of Sequences: 59808
Number of extensions: 557762
Number of successful extensions: 1475
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1360
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1471
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1925890720
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)