Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0405
(685 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q16MK2 Cluster: Eukaryotic translation initiation facto... 83 5e-15
UniRef50_A2I3V2 Cluster: Eukaryotic translation initiation facto... 81 2e-14
UniRef50_A7RSH7 Cluster: Predicted protein; n=1; Nematostella ve... 71 3e-11
UniRef50_Q7K550 Cluster: GH12681p; n=5; Sophophora|Rep: GH12681p... 69 8e-11
UniRef50_O75822 Cluster: Eukaryotic translation initiation facto... 65 2e-09
UniRef50_UPI0000584E77 Cluster: PREDICTED: similar to MGC81283 p... 63 5e-09
UniRef50_A6N001 Cluster: Eukaryotic translation initiation facto... 40 0.056
UniRef50_Q00ZU9 Cluster: Translation initiation factor eIF3, p35... 36 0.92
UniRef50_A5N4G0 Cluster: Predicted exonuclease, sbcC related; n=... 34 3.7
UniRef50_P08799 Cluster: Myosin-2 heavy chain, non muscle; n=5; ... 34 3.7
UniRef50_A2EH37 Cluster: Putative uncharacterized protein; n=1; ... 33 6.5
>UniRef50_Q16MK2 Cluster: Eukaryotic translation initiation factor 3
subunit 1; n=7; Endopterygota|Rep: Eukaryotic
translation initiation factor 3 subunit 1 - Aedes
aegypti (Yellowfever mosquito)
Length = 241
Score = 83.4 bits (197), Expect = 5e-15
Identities = 52/141 (36%), Positives = 67/141 (47%), Gaps = 2/141 (1%)
Frame = +3
Query: 267 MDVSWDADNFEPKLPTTLAAS--NKWEGEDEDDNVXXXXXXXXXXXXXXXXXXAAXXXXX 440
M+ W+ + + K+P NKW+GEDE++ V
Sbjct: 1 MEEDWEQLSEQEKVPIPAKKPDVNKWDGEDEEEEVKDSWEDEDELEEKKDEEKVETPKAK 60
Query: 441 XXXXXIHDKIXXXXXXXXXXXXXLVTEKTAEEMTPEQKLAEKLRQQKLQEESDLRLAMET 620
+ KI EK E+MTPEQKLAEKLR QKLQEESDL+ A++T
Sbjct: 61 PKKT-LQQKIADKEKLKQEEAERRRLEKEEEDMTPEQKLAEKLRLQKLQEESDLKNALDT 119
Query: 621 FGVTEGNIGKLDNFHPTTKEE 683
FGVT +G +D HPT KEE
Sbjct: 120 FGVT-SIVGGIDGMHPTNKEE 139
>UniRef50_A2I3V2 Cluster: Eukaryotic translation initiation factor 3
subunit 1-like protein; n=1; Maconellicoccus
hirsutus|Rep: Eukaryotic translation initiation factor 3
subunit 1-like protein - Maconellicoccus hirsutus
(hibiscus mealybug)
Length = 237
Score = 81.4 bits (192), Expect = 2e-14
Identities = 50/140 (35%), Positives = 68/140 (48%), Gaps = 3/140 (2%)
Frame = +3
Query: 273 VSWDADNFEPKLPTTLAASNKWEGEDEDDNVXXXXXXXXXXXXXXXXXXAAXXXXXXXXX 452
+ WD++NFEPK P + +NKW+GEDEDDN+ +
Sbjct: 1 MDWDSENFEPK-PIASSVNNKWDGEDEDDNIKDNWEDDEDTRKDDSQRRQSAQSKKPKSR 59
Query: 453 X---IHDKIXXXXXXXXXXXXXLVTEKTAEEMTPEQKLAEKLRQQKLQEESDLRLAMETF 623
I ++ L + MTPE+ AEKLR+QKL EE DL A +T
Sbjct: 60 LEEAIEERERKERERKSKLRSELEKDIDTTNMTPEEIQAEKLRRQKLLEEVDLEDARDTL 119
Query: 624 GVTEGNIGKLDNFHPTTKEE 683
G+ EG+ G LD F+PTT+EE
Sbjct: 120 GL-EGDSGFLDGFNPTTEEE 138
>UniRef50_A7RSH7 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 247
Score = 70.5 bits (165), Expect = 3e-11
Identities = 46/143 (32%), Positives = 65/143 (45%), Gaps = 8/143 (5%)
Frame = +3
Query: 279 WDADNFEPKLPTTLAASNKWEGEDEDDNVXXXXXXXXXXXXXXXXXXAAXXXXXXXXXXI 458
WD + FEP ++KWEGEDEDD++ +
Sbjct: 4 WDDEKFEPGEVPADGVTDKWEGEDEDDDIKESWDDDDEDEKKEDEAKNTEAAAPKKKKTL 63
Query: 459 HDKIXXXXXXXXXXXXXLVTEKTA-----EEMTPEQKLAEKLRQQKLQEESDLRLAMETF 623
+ EK +E+TPE+++AEKLR+QK+ EESDL +AM+TF
Sbjct: 64 KQILKEKEEQKLLEEKRKAEEKQKLEEEDKELTPEEQMAEKLRRQKIVEESDLLVAMDTF 123
Query: 624 GV---TEGNIGKLDNFHPTTKEE 683
GV E + LD+ P+TKEE
Sbjct: 124 GVGTQEEASRTGLDSMIPSTKEE 146
>UniRef50_Q7K550 Cluster: GH12681p; n=5; Sophophora|Rep: GH12681p -
Drosophila melanogaster (Fruit fly)
Length = 236
Score = 69.3 bits (162), Expect = 8e-11
Identities = 44/125 (35%), Positives = 56/125 (44%)
Frame = +3
Query: 309 PTTLAASNKWEGEDEDDNVXXXXXXXXXXXXXXXXXXAAXXXXXXXXXXIHDKIXXXXXX 488
PT A+ NKWEGEDED+++ + K+
Sbjct: 17 PTAAASVNKWEGEDEDEDIKDSWEDEEEKKDEEKPTKTEAPAKPKPNKALKAKLEQQALL 76
Query: 489 XXXXXXXLVTEKTAEEMTPEQKLAEKLRQQKLQEESDLRLAMETFGVTEGNIGKLDNFHP 668
K ++P +KLAEKLR QK+QE SDL+ A E FGVT G LD F+P
Sbjct: 77 EEE-----AEAKRLANLSPAEKLAEKLRLQKIQEASDLKHAQEAFGVT-STCGGLDAFNP 130
Query: 669 TTKEE 683
TKEE
Sbjct: 131 ETKEE 135
>UniRef50_O75822 Cluster: Eukaryotic translation initiation factor 3
subunit 1; n=36; Euteleostomi|Rep: Eukaryotic
translation initiation factor 3 subunit 1 - Homo sapiens
(Human)
Length = 258
Score = 64.9 bits (151), Expect = 2e-09
Identities = 45/144 (31%), Positives = 65/144 (45%), Gaps = 8/144 (5%)
Frame = +3
Query: 276 SWDADNFEPKLPTT------LAASNKWEGEDEDDNVXXXXXXXXXXXXXXXXXX--AAXX 431
SWDAD F + P A ++WEGEDED++V
Sbjct: 13 SWDADAFSVEDPVRKVGGGGTAGGDRWEGEDEDEDVKDNWDDDDDEKKEEAEVKPEVKIS 72
Query: 432 XXXXXXXXIHDKIXXXXXXXXXXXXXLVTEKTAEEMTPEQKLAEKLRQQKLQEESDLRLA 611
I +K L + + +TPE++LA+KLR +KLQEESDL LA
Sbjct: 73 EKKKIAEKIKEKERQQKKRQEEIKKRLEEPEEPKVLTPEEQLADKLRLKKLQEESDLELA 132
Query: 612 METFGVTEGNIGKLDNFHPTTKEE 683
ETFGV G +D +P+++++
Sbjct: 133 KETFGVNNAVYG-IDAMNPSSRDD 155
>UniRef50_UPI0000584E77 Cluster: PREDICTED: similar to MGC81283
protein; n=1; Strongylocentrotus purpuratus|Rep:
PREDICTED: similar to MGC81283 protein -
Strongylocentrotus purpuratus
Length = 241
Score = 63.3 bits (147), Expect = 5e-09
Identities = 42/139 (30%), Positives = 62/139 (44%), Gaps = 4/139 (2%)
Frame = +3
Query: 279 WDADNFEPKLPTT---LAASNKWEGEDEDDNVXXXXXXXXXXXXXXXXXXAAXXXXXXXX 449
W+ ++F+P + AA +KWEGEDE++NV
Sbjct: 4 WEDEDFDPDAGFSKAGAAAGDKWEGEDEEENVKDDWAASDDEEKKDESSEQKSTSGDGAF 63
Query: 450 XXIHDKIXXXXXXXXXXXXXL-VTEKTAEEMTPEQKLAEKLRQQKLQEESDLRLAMETFG 626
K EK ++TPE+++ EKL QQK+ EE+DL LA E FG
Sbjct: 64 QVKKKKNTLEKALQRKAEKRKDKVEKEKIKLTPEEEMDEKLLQQKMVEEADLELAKEAFG 123
Query: 627 VTEGNIGKLDNFHPTTKEE 683
V G+ +D +PTT+E+
Sbjct: 124 VGAGDGKSIDAMNPTTEED 142
>UniRef50_A6N001 Cluster: Eukaryotic translation initiation factor
3, subunit 1 alpha; n=8; Magnoliophyta|Rep: Eukaryotic
translation initiation factor 3, subunit 1 alpha - Oryza
sativa subsp. indica (Rice)
Length = 151
Score = 39.9 bits (89), Expect = 0.056
Identities = 18/41 (43%), Positives = 26/41 (63%)
Frame = +3
Query: 561 EKLRQQKLQEESDLRLAMETFGVTEGNIGKLDNFHPTTKEE 683
EKLRQQ+L EE+D + E FG +G+ LD F P ++ +
Sbjct: 10 EKLRQQRLVEEADFKSTTELFGKKDGSEKSLDTFIPKSESD 50
>UniRef50_Q00ZU9 Cluster: Translation initiation factor eIF3, p35
subunit; n=2; Ostreococcus|Rep: Translation initiation
factor eIF3, p35 subunit - Ostreococcus tauri
Length = 236
Score = 35.9 bits (79), Expect = 0.92
Identities = 20/40 (50%), Positives = 25/40 (62%)
Frame = +3
Query: 558 AEKLRQQKLQEESDLRLAMETFGVTEGNIGKLDNFHPTTK 677
AEK R+Q+L EE+DLR A E FG G +L+ F P K
Sbjct: 88 AEKARRQRLVEEADLRAARELFG---GGDVELETFEPKGK 124
>UniRef50_A5N4G0 Cluster: Predicted exonuclease, sbcC related; n=1;
Clostridium kluyveri DSM 555|Rep: Predicted exonuclease,
sbcC related - Clostridium kluyveri DSM 555
Length = 1164
Score = 33.9 bits (74), Expect = 3.7
Identities = 17/50 (34%), Positives = 27/50 (54%)
Frame = +3
Query: 513 VTEKTAEEMTPEQKLAEKLRQQKLQEESDLRLAMETFGVTEGNIGKLDNF 662
V E+ ++ EQKL EKLRQ+K+ E+D+ E F + +N+
Sbjct: 346 VKEQKIKDAIEEQKLLEKLRQEKILLENDIESLKEKFNNKNDQVITNENY 395
>UniRef50_P08799 Cluster: Myosin-2 heavy chain, non muscle; n=5;
Dictyostelium discoideum|Rep: Myosin-2 heavy chain, non
muscle - Dictyostelium discoideum (Slime mold)
Length = 2116
Score = 33.9 bits (74), Expect = 3.7
Identities = 20/58 (34%), Positives = 28/58 (48%)
Frame = +3
Query: 510 LVTEKTAEEMTPEQKLAEKLRQQKLQEESDLRLAMETFGVTEGNIGKLDNFHPTTKEE 683
LV EK + +K A +QQ+L E+DLR + + + I KL H TK E
Sbjct: 2035 LVVEKLETDYKRAKKEAADEQQQRLTVENDLRKHLSEISLLKDAIDKLQRDHDKTKRE 2092
>UniRef50_A2EH37 Cluster: Putative uncharacterized protein; n=1;
Trichomonas vaginalis G3|Rep: Putative uncharacterized
protein - Trichomonas vaginalis G3
Length = 960
Score = 33.1 bits (72), Expect = 6.5
Identities = 20/48 (41%), Positives = 29/48 (60%)
Frame = +3
Query: 222 KSFHINSAADSGVLKMDVSWDADNFEPKLPTTLAASNKWEGEDEDDNV 365
KSFH +S +DS +L + V AD FEP+ + L A K E ++ D +V
Sbjct: 512 KSFH-DSDSDSDLLPLFVPPVADEFEPQPISVLLARAKLESDESDIDV 558
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 575,496,290
Number of Sequences: 1657284
Number of extensions: 9864198
Number of successful extensions: 30679
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 29414
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30656
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 53305790091
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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