Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0405
         (685 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g66070.1 68414.m07499 translation initiation factor-related s...    42   3e-04
At5g37475.1 68418.m04510 translation initiation factor-related s...    33   0.13 
At1g61840.1 68414.m06978 DC1 domain-containing protein similar t...    30   1.6  
At4g26120.1 68417.m03760 ankyrin repeat family protein / BTB/POZ...    29   2.9  
At4g27850.1 68417.m03999 proline-rich family protein contains pr...    28   5.0  
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329...    28   5.0  
At3g50590.1 68416.m05533 transducin family protein / WD-40 repea...    28   6.6  
At3g47440.1 68416.m05158 major intrinsic family protein / MIP fa...    27   8.8  
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460...    27   8.8  
At1g76770.1 68414.m08934 heat shock protein-related contains sim...    27   8.8  

>At1g66070.1 68414.m07499 translation initiation factor-related
           similar to Eukaryotic translation initiation factor 3
           subunit 1 (eIF-3 alpha) (eIF3 p35) (eIF3j)
           (Swiss-Prot:O75822) [Homo sapiens]
          Length = 226

 Score = 42.3 bits (95), Expect = 3e-04
 Identities = 23/54 (42%), Positives = 32/54 (59%)
 Frame = +3

Query: 522 KTAEEMTPEQKLAEKLRQQKLQEESDLRLAMETFGVTEGNIGKLDNFHPTTKEE 683
           K A +  P   +AEKLRQQ+L EE+D R   E FGV + +   LD F P ++ +
Sbjct: 76  KEAPKEKPLDPIAEKLRQQRLVEEADYRATAELFGVKDDD-KNLDMFIPKSESD 128


>At5g37475.1 68418.m04510 translation initiation factor-related
           similar to Eukaryotic translation initiation factor 3
           subunit 1 (eIF-3 alpha) (eIF3 p35) (eIF3j)
           (Swiss-Prot:O75822) [Homo sapiens]
          Length = 225

 Score = 33.5 bits (73), Expect = 0.13
 Identities = 28/118 (23%), Positives = 46/118 (38%), Gaps = 1/118 (0%)
 Frame = +3

Query: 279 WDADNFEPKLPTTLAASNKWEGEDEDD-NVXXXXXXXXXXXXXXXXXXAAXXXXXXXXXX 455
           W+A++F+P LP+ +   + W+ ED D+ ++                  A+          
Sbjct: 4   WEAEDFQP-LPSKVELKSNWDDEDVDENDIKDSWEEEDVSAPPPIVKPASEKAPKKPAVK 62

Query: 456 IHDKIXXXXXXXXXXXXXLVTEKTAEEMTPEQKLAEKLRQQKLQEESDLRLAMETFGV 629
             +K              +   K      P   +AEKLR Q+L EE+D +   E FGV
Sbjct: 63  AVEK----------KVKTVEAPKGTSREEPLDPIAEKLRMQRLVEEADYQSTAELFGV 110


>At1g61840.1 68414.m06978 DC1 domain-containing protein similar to
           hypothetical protein GI:3184279 from [Arabidopsis
           thaliana]; contains Pfam profile PF03107: DC1 domain
          Length = 814

 Score = 29.9 bits (64), Expect = 1.6
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = +2

Query: 176 SDKSLSIVVRFWCSGEKFSY*FGC*FWSPKN 268
           SD+ LS++  FWC+ ++F+   GC   S  N
Sbjct: 278 SDEDLSVLPLFWCNNKEFNVDGGCDICSGSN 308


>At4g26120.1 68417.m03760 ankyrin repeat family protein / BTB/POZ
           domain-containing protein contains Pfam domain, PF00023:
           Ankyrin repeat and Pfam domain, PF00651: BTB/POZ domain
          Length = 600

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 18/50 (36%), Positives = 26/50 (52%)
 Frame = +3

Query: 525 TAEEMTPEQKLAEKLRQQKLQEESDLRLAMETFGVTEGNIGKLDNFHPTT 674
           + EE TPE++L +K R  +LQE       M+TF   +   GK     PT+
Sbjct: 527 SVEEDTPEKRLQKKQRYMELQE-----TLMKTFSEDKEECGKSSTPKPTS 571


>At4g27850.1 68417.m03999 proline-rich family protein contains
           proline-rich extensin domains, INTERPRO:IPR002965
          Length = 577

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 15/38 (39%), Positives = 21/38 (55%)
 Frame = +2

Query: 515 DREDCRRNDSRAEVG*ETSPAEATGRIRFATSHGNLWC 628
           +RE+   N+S+ E     SP EA   IR   +H NL+C
Sbjct: 28  EREENNNNESQEETRTSRSPNEALDEIRRQQTH-NLYC 64


>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
           Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
          Length = 339

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = -3

Query: 356 ILVFPFPFVGGCQRGREFGLEV 291
           ++VFP  F+GG  RG  FGL V
Sbjct: 53  LVVFPEAFIGGYPRGFRFGLGV 74


>At3g50590.1 68416.m05533 transducin family protein / WD-40 repeat
            family protein contains 3 WD-40 repeats (PF00400); some
            similarity to s-tomosyn isoform (GI:4689231)[Rattus
            norvegicus]; contains non-consensus AT-AC splice sites at
            intron 18
          Length = 1606

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +3

Query: 237  NSAADSGVLKMDVSWDADNFEPKLPTTLAASNKWE 341
            N AA+S V K    W   N+E K PT + +  KWE
Sbjct: 991  NFAAESMVPK-GPEWGGGNWEIKTPTDMKSIPKWE 1024


>At3g47440.1 68416.m05158 major intrinsic family protein / MIP
           family protein contains Pfam profile: MIP PF00230
          Length = 256

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 17/57 (29%), Positives = 28/57 (49%)
 Frame = -1

Query: 682 SSFVVGWKLSSLPMLPSVTPKVSMASRKSDSSCSFCWRSFSANFCSGVISSAVFSVT 512
           SS  + W +S   + P+VT  +++A R S  +  F W S      + V++  V  VT
Sbjct: 73  SSVYISWNVSGGHVNPAVTFAMAVAGRISVPTAMFYWTS---QMIASVMACLVLKVT 126


>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
           Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
          Length = 346

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 11/22 (50%), Positives = 15/22 (68%)
 Frame = -3

Query: 356 ILVFPFPFVGGCQRGREFGLEV 291
           +++FP  F+GG  RG  FGL V
Sbjct: 60  LVLFPEAFIGGYPRGFRFGLAV 81


>At1g76770.1 68414.m08934 heat shock protein-related contains
           similarity to 17.9 kDa heat-shock protein [Helianthus
           annuus] gi|11990130|emb|CAB55634
          Length = 244

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 10/30 (33%), Positives = 21/30 (70%)
 Frame = +3

Query: 510 LVTEKTAEEMTPEQKLAEKLRQQKLQEESD 599
           +V EKT E+  PE+++ E+ + ++  EE++
Sbjct: 150 IVEEKTEEKTEPEEEIKEETKPEEENEEAE 179


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,855,991
Number of Sequences: 28952
Number of extensions: 229833
Number of successful extensions: 684
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 675
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 684
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)