BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0401 (668 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 128 7e-31 SPAC17H9.02 |||ATP-dependent RNA helicase Mtr4-like |Schizosacch... 101 9e-23 SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 29 0.46 SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharo... 29 0.80 SPAC13A11.01c |rga8|SPAC2F7.18c|GTPase activating protein Rga8 |... 27 2.4 SPCC330.08 |alg11|gmd3|alpha-1,2-mannosyltransferase Alg11|Schiz... 27 3.2 SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 4.3 SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 26 5.6 SPAC11E3.13c |||1,3-beta-glucanosyltransferase |Schizosaccharomy... 26 5.6 SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual 26 5.6 SPAC23G3.11 |rpn6||19S proteasome regulatory subunit Rpn6|Schizo... 25 9.9 SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 25 9.9 SPAC6B12.12 |tom70||mitochondrial TOM complex subunit Tom70|Schi... 25 9.9 >SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1117 Score = 128 bits (309), Expect = 7e-31 Identities = 75/223 (33%), Positives = 120/223 (53%), Gaps = 6/223 (2%) Frame = +3 Query: 15 YSTIRTQLDLLGDQFRSFITKPEYLKPFLQPGRLVKVKTEKYEYDWGIIVNFRHKSNKGK 194 Y T++TQL+ R+ + P + FLQ GRLV+VK ++DWG++VN + Sbjct: 694 YHTLKTQLERYRTDVRTVVNHPNFCLSFLQGGRLVRVKVGNEDFDWGVVVNVSKRPLP-- 751 Query: 195 KGQEENPLTAEPAIIVDILLHVKTATGG----DGETNVPCPPG--ESGDVQVVPVVHTLI 356 KGQ L E + IV L+ V + TG G PP + G +VVP + + + Sbjct: 752 KGQSNEYLPQE-SYIVHTLVMVASDTGPLRIRSGHLPEVHPPAAEDKGKFEVVPFLLSSL 810 Query: 357 YQISSLRVYYPKDLRPSDNRKSVLKTIGEVKKRFPDGPPLLNPINDMKINDSVFKQCVDR 536 I+ +RV+ P DL+ + +V K + EVK+RFP+G LL+P+ +M I + F + + + Sbjct: 811 DGIAHIRVFLPNDLKSQGQKLTVGKALSEVKRRFPEGITLLDPVENMNIKEPTFIKLMKK 870 Query: 537 IKLLEERLYSHPIHTDKNRSALTAAYDKKQELYEELQNAKAEL 665 + +LE RL S+P+H A Y +K L EE+++ K +L Sbjct: 871 VNILESRLLSNPLHNFSELEEKYAEYLRKLALLEEVKDLKKKL 913 >SPAC17H9.02 |||ATP-dependent RNA helicase Mtr4-like |Schizosaccharomyces pombe|chr 1|||Manual Length = 1030 Score = 101 bits (242), Expect = 9e-23 Identities = 54/225 (24%), Positives = 116/225 (51%), Gaps = 4/225 (1%) Frame = +3 Query: 3 SVASYSTIRTQLDLLGDQFRSFITKPEYLKPFLQPGRLVKVKTEKYEYDWGIIVNFRHKS 182 +V + ++ QL+ G++ + +T P+ P+LQ GRL+++K + WG++VN + Sbjct: 606 AVKEFHDLKLQLEKYGEEIQKVMTHPDNCLPYLQSGRLIQIKLGGIIFPWGVLVNVIKRE 665 Query: 183 NKGKKGQEENPLTAEPAIIVDILLHVKTATGGDGETN----VPCPPGESGDVQVVPVVHT 350 ++ P ++D+LL + + + + + N VP P E+ ++V V+ T Sbjct: 666 FDPNTREQVAP---HETYVLDVLLPISSNSMSNHKVNPSILVPPRPNETPLYEIVSVLLT 722 Query: 351 LIYQISSLRVYYPKDLRPSDNRKSVLKTIGEVKKRFPDGPPLLNPINDMKINDSVFKQCV 530 + ISS+R+Y P++L ++++ + + EV + F + P L+P+ M I S + Sbjct: 723 AVCNISSIRIYMPRELNSNESKLRAYRRVNEVIEEFKE-IPYLDPLEHMHIESSTLSLSL 781 Query: 531 DRIKLLEERLYSHPIHTDKNRSALTAAYDKKQELYEELQNAKAEL 665 ++++LE +L+ P + D A + KK L ++++ ++ Sbjct: 782 RKLEILEPKLFDSPYYKDSKHRAEYHEFRKKLNLRAQIKDISTKI 826 >SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosaccharomyces pombe|chr 2|||Manual Length = 2358 Score = 29.5 bits (63), Expect = 0.46 Identities = 34/109 (31%), Positives = 53/109 (48%), Gaps = 5/109 (4%) Frame = +3 Query: 321 DVQVVPVVHTLIYQISSLRVYYPKDLRPSD----NRKSVLKTIGEVKKRFPDGPPLLNPI 488 D+QV ++ ++SS V +DLR SD N SVL+TI V R P + N + Sbjct: 1728 DLQVSSDINVRNDRLSSF-VMSSEDLRSSDGHPENSDSVLETISSVHHRSPINQVVRN-L 1785 Query: 489 NDMKIN-DSVFKQCVDRIKLLEERLYSHPIHTDKNRSALTAAYDKKQEL 632 N+ +++ DSV +++ L + + TD N AL K +EL Sbjct: 1786 NESQLSLDSVISMNLNKEFALTK---TENDFTDDNGRALNYFSQKLEEL 1831 >SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharomyces pombe|chr 2|||Manual Length = 209 Score = 28.7 bits (61), Expect = 0.80 Identities = 18/88 (20%), Positives = 37/88 (42%) Frame = +3 Query: 357 YQISSLRVYYPKDLRPSDNRKSVLKTIGEVKKRFPDGPPLLNPINDMKINDSVFKQCVDR 536 Y + YP R +++R+ + + E+KK P G ++ +D I + + + Sbjct: 61 YVTGGVEKLYPDPKRENEDRELTSEELDELKKSLPPGQAIVRRDDDGNIIEIIHGEAKTL 120 Query: 537 IKLLEERLYSHPIHTDKNRSALTAAYDK 620 +L++ + P T+ R A K Sbjct: 121 DDVLDKEISIAPAKTEVVRQLEEEAVKK 148 >SPAC13A11.01c |rga8|SPAC2F7.18c|GTPase activating protein Rga8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 777 Score = 27.1 bits (57), Expect = 2.4 Identities = 16/43 (37%), Positives = 23/43 (53%) Frame = +1 Query: 532 IESSYWKKDYTHIRSIQTKTEAP*QLLTTRNKSYMKSYRTLKR 660 I + + K H + +QT Q+LT + KSY K Y TLK+ Sbjct: 104 IIAPFSKMSIDHSQKLQTSQ----QVLTNQIKSYEKKYYTLKK 142 >SPCC330.08 |alg11|gmd3|alpha-1,2-mannosyltransferase Alg11|Schizosaccharomyces pombe|chr 3|||Manual Length = 471 Score = 26.6 bits (56), Expect = 3.2 Identities = 17/75 (22%), Positives = 34/75 (45%) Frame = +3 Query: 255 HVKTATGGDGETNVPCPPGESGDVQVVPVVHTLIYQISSLRVYYPKDLRPSDNRKSVLKT 434 H+ + G D + +V PP + +++ + I + + Y RP N ++VL++ Sbjct: 243 HIASLWGKDIQLSVVFPPCNTSELEKID-----INRKREPTLLYLAQYRPEKNHENVLRS 297 Query: 435 IGEVKKRFPDGPPLL 479 ++ PD P L Sbjct: 298 FALYFEQHPDSPAKL 312 >SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 536 Score = 26.2 bits (55), Expect = 4.3 Identities = 30/121 (24%), Positives = 47/121 (38%) Frame = -2 Query: 559 SLSSNNLILSTHCLNTESLIFISLMGFNSGGPSGNLFFTSPIVFNTDFRLSEGLKSLG*Y 380 SLSS+++I S+ S ++ +S P + FTSP + L Sbjct: 34 SLSSSSVIPSSSSSMLSSSSATAISSSSSSSPLSSSSFTSPASSSFITSLVSSSSQQSSS 93 Query: 379 TRRELIWYISVCTTGTTCTSPDSPGGHGTLVSPSPPVAVFTCSRMSTIMAGSAVKGFSSC 200 + L S T ++ SP S L S S + S S+ M+ S++ SS Sbjct: 94 SSASLTSSSSATLTSSSSASPTSSSSSHALSSSS---SSLVASSSSSGMSSSSLSHSSSV 150 Query: 199 P 197 P Sbjct: 151 P 151 >SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2310 Score = 25.8 bits (54), Expect = 5.6 Identities = 18/85 (21%), Positives = 37/85 (43%) Frame = +3 Query: 294 VPCPPGESGDVQVVPVVHTLIYQISSLRVYYPKDLRPSDNRKSVLKTIGEVKKRFPDGPP 473 + C S +++ P++ T+I + + D L G V+K PP Sbjct: 1439 IKCGQLLSSKLRLGPLLTTVIKLVIEYSQAKHAAIILKDASNYTLAAHGNVEKAESFEPP 1498 Query: 474 LLNPINDMKINDSVFKQCVDRIKLL 548 ++ +D+KI DS+ + D +++ Sbjct: 1499 VILSQSDVKIPDSLLSEVFDHCRIV 1523 >SPAC11E3.13c |||1,3-beta-glucanosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 510 Score = 25.8 bits (54), Expect = 5.6 Identities = 12/26 (46%), Positives = 15/26 (57%) Frame = -2 Query: 154 PQSYSYFSVFTLTSLPGCKKGFKYSG 77 P SYF+ F L ++P KGF SG Sbjct: 354 PADDSYFTSFPLPTMPSEAKGFIESG 379 >SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual Length = 935 Score = 25.8 bits (54), Expect = 5.6 Identities = 19/60 (31%), Positives = 31/60 (51%), Gaps = 3/60 (5%) Frame = -1 Query: 227 LGRQRILFLSFLAFVRFMSEVHYDA---PVVFVFLCFHLDKSSRLQKRLQVLRLSYERPK 57 L R ILFL+F + +S + ++A V+F+ L ++ + LQK + L Y R K Sbjct: 722 LDRLAILFLTFSPTMCMLS-ISFEALFYVVLFITLGLWMELETELQKYTEQLHPEYSRKK 780 >SPAC23G3.11 |rpn6||19S proteasome regulatory subunit Rpn6|Schizosaccharomyces pombe|chr 1|||Manual Length = 421 Score = 25.0 bits (52), Expect = 9.9 Identities = 17/61 (27%), Positives = 31/61 (50%), Gaps = 1/61 (1%) Frame = +3 Query: 456 FPDGPPLLNPI-NDMKINDSVFKQCVDRIKLLEERLYSHPIHTDKNRSALTAAYDKKQEL 632 + D L+N + +++K D K + + LLE ++Y + K R++LTAA + Sbjct: 141 YTDAINLINTLLSELKRMDD--KMLLTEVHLLESKVYHAIRNIPKARASLTAAKTSANAV 198 Query: 633 Y 635 Y Sbjct: 199 Y 199 >SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1190 Score = 25.0 bits (52), Expect = 9.9 Identities = 14/47 (29%), Positives = 24/47 (51%) Frame = +3 Query: 393 DLRPSDNRKSVLKTIGEVKKRFPDGPPLLNPINDMKINDSVFKQCVD 533 D++ + + SV ++I EV DGPP+L + + V + VD Sbjct: 959 DMQFTSEKPSVDESITEVDDAIKDGPPVLAETLTNSLMEDVGPENVD 1005 >SPAC6B12.12 |tom70||mitochondrial TOM complex subunit Tom70|Schizosaccharomyces pombe|chr 1|||Manual Length = 625 Score = 25.0 bits (52), Expect = 9.9 Identities = 10/35 (28%), Positives = 18/35 (51%) Frame = +3 Query: 426 LKTIGEVKKRFPDGPPLLNPINDMKINDSVFKQCV 530 +KT + KKRFP+ + N ++ ++ F V Sbjct: 469 MKTFEDCKKRFPNSSEVYNYFGEILLDQQKFDDAV 503 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,642,783 Number of Sequences: 5004 Number of extensions: 56419 Number of successful extensions: 169 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 164 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 167 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 305854096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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