Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0401
         (668 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g06990.1 68415.m00800 HUA enhancer 2 (HEN2) / DExH-box RNA he...   136   9e-33
At1g59760.1 68414.m06729 ATP-dependent RNA helicase, putative si...    87   1e-17
At2g31850.1 68415.m03889 expressed protein                             31   0.92 
At2g17090.1 68415.m01973 protein kinase family protein similar t...    29   2.1  
At4g29220.1 68417.m04180 phosphofructokinase family protein simi...    28   6.5  
At3g22430.1 68416.m02834 expressed protein                             28   6.5  
At1g35320.1 68414.m04378 expressed protein                             28   6.5  
At3g45590.1 68416.m04924 tRNA-splicing endonuclease, putative co...    27   8.5  

>At2g06990.1 68415.m00800 HUA enhancer 2 (HEN2) / DExH-box RNA
            helicase, putative nearly identical to HUA enhancer 2
            [Arabidopsis thaliana] GI:16024936
          Length = 995

 Score =  136 bits (330), Expect = 9e-33
 Identities = 76/222 (34%), Positives = 121/222 (54%), Gaps = 2/222 (0%)
 Frame = +3

Query: 6    VASYSTIRTQLDLLGDQFRSFITKPEYLKPFLQPGRLVKVKTEKYEYDWGIIVNFRHKSN 185
            VA Y  ++  +     +  S I +PE +  FL  GRLVK++    ++ WG++VN    S+
Sbjct: 575  VAEYHNLQFDIAKHEKKLMSEIIRPERVLCFLDTGRLVKIREGGTDWGWGVVVNVVKNSS 634

Query: 186  KGKKGQEENPLTAEPAIIVDILLHVKTATGGDGETNVPCPP--GESGDVQVVPVVHTLIY 359
             G      +        IVD LLH  T    +G    PCPP  GE G++ VVPV   LI 
Sbjct: 635  VGTGSASSHG----GGYIVDTLLHCSTGFSENGAKPKPCPPRAGEKGEMHVVPVQLPLIS 690

Query: 360  QISSLRVYYPKDLRPSDNRKSVLKTIGEVKKRFPDGPPLLNPINDMKINDSVFKQCVDRI 539
             +S LR+  P DLRP + R+S+L  + E+  RFP G P L+P+ DM I D+     V +I
Sbjct: 691  ALSRLRISVPSDLRPVEARQSILLALQELSSRFPLGFPKLHPVKDMNIQDTEIVDLVSQI 750

Query: 540  KLLEERLYSHPIHTDKNRSALTAAYDKKQELYEELQNAKAEL 665
            + +E++L +HP+H  ++   +  ++ +K E+  E+Q  K+++
Sbjct: 751  EEVEQKLLAHPMHKSEDDQQI-KSFQRKAEVNYEIQQLKSKM 791


>At1g59760.1 68414.m06729 ATP-dependent RNA helicase, putative similar
            to SP|P47047 ATP-dependent RNA helicase DOB1
            {Saccharomyces cerevisiae}, HUA enhancer 2 [Arabidopsis
            thaliana] GI:16024936; contains Pfam profiles PF00270:
            DEAD/DEAH box helicase, PF00271: Helicase conserved
            C-terminal domain
          Length = 988

 Score = 86.6 bits (205), Expect = 1e-17
 Identities = 66/230 (28%), Positives = 103/230 (44%), Gaps = 12/230 (5%)
 Frame = +3

Query: 3    SVASYSTIRTQLDLLGDQFRSFITKPEYLKPFLQPGRLVKV-------KTEKYEYD---- 149
            S+ +Y  +  Q   L    R  +  P+Y  PFL P R V +       + + +  +    
Sbjct: 556  SLKNYYNLILQYKSLKKDIREIVFTPKYCLPFLLPNRAVCLDCTNDDEEPQSFSIEDQDT 615

Query: 150  WGIIVNFRHKSNKGKKGQEENPLTAEPAIIVDILLHVKTATGGDGETNVPCPP-GESGDV 326
            WG+I+ F    +  +      P  A     VD+L     +  G G+  V   P  E G+ 
Sbjct: 616  WGVIMKFNKVKSLSEDDDSRRPEDAN--YTVDVLTRCMVSKDGVGKKKVKAVPIKERGEP 673

Query: 327  QVVPVVHTLIYQISSLRVYYPKDLRPSDNRKSVLKTIGEVKKRFPDGPPLLNPINDMKIN 506
             VV V  + I  +SS  +  PKDL P + R++ LK + E+  R PDG P L+P  DMKI 
Sbjct: 674  VVVTVPLSQIKSLSSAIMNIPKDLVPLEARENALKKVSELLSRHPDGIP-LDPEVDMKIK 732

Query: 507  DSVFKQCVDRIKLLEERLYSHPIHTDKNRSALTAAYDKKQELYEELQNAK 656
             S +K+ V R++ LE     H I      +        K+EL  ++++ K
Sbjct: 733  SSSYKKTVRRLEALENLFEKHKIAKSPLITEKLKVLQMKEELIAKIKSLK 782


>At2g31850.1 68415.m03889 expressed protein
          Length = 113

 Score = 30.7 bits (66), Expect = 0.92
 Identities = 20/48 (41%), Positives = 28/48 (58%), Gaps = 2/48 (4%)
 Frame = -2

Query: 355 ISVCTTGTTCTSPDSPGGHGTLVS--PSPPVAVFTCSRMSTIMAGSAV 218
           +S  +TG+ C+SPDS  G  ++ +  PSP  A   C   ST  +GSAV
Sbjct: 67  LSGSSTGS-CSSPDSSTGSNSVPAPVPSPAPAGSCCCCSSTADSGSAV 113


>At2g17090.1 68415.m01973 protein kinase family protein similar to
           Arabidopsis thaliana APK1A [SP|Q06548], APK1B
           [SP|P46573]; contains Pfam profile: PF00069 Protein
           kinase domain
          Length = 465

 Score = 29.5 bits (63), Expect = 2.1
 Identities = 14/34 (41%), Positives = 20/34 (58%)
 Frame = +1

Query: 499 KLMTQCLNNASIESSYWKKDYTHIRSIQTKTEAP 600
           KL +QCL     ES   K+    + ++QT+TEAP
Sbjct: 265 KLASQCLKYEGQESPNTKEIVATLETLQTRTEAP 298


>At4g29220.1 68417.m04180 phosphofructokinase family protein similar
           to phosphofructokinase [Amycolatopsis methanolica]
           GI:17432243; contains Pfam profile PF00365:
           Phosphofructokinase
          Length = 473

 Score = 27.9 bits (59), Expect = 6.5
 Identities = 19/79 (24%), Positives = 31/79 (39%)
 Frame = +3

Query: 423 VLKTIGEVKKRFPDGPPLLNPINDMKINDSVFKQCVDRIKLLEERLYSHPIHTDKNRSAL 602
           +L+ +      FPD P   NP+ D      V +  VD    + +++  HP   D  R   
Sbjct: 20  ILEDVPHFSDDFPDHPTYPNPLQDNAAYSVVKQYFVDEDDTVPQKIVVHP---DSPRGTH 76

Query: 603 TAAYDKKQELYEELQNAKA 659
                 +Q +Y E  +  A
Sbjct: 77  FRRAGPRQRVYFESDDVLA 95


>At3g22430.1 68416.m02834 expressed protein
          Length = 342

 Score = 27.9 bits (59), Expect = 6.5
 Identities = 18/50 (36%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
 Frame = -1

Query: 314 LAGRTRHVGLAVAAGRRLHVQ*DVDNNGGLGRQRI-LFLSFLAFVRFMSE 168
           +AG +R VG  V A + + V     +NGG    ++ L  SFL FV+ + E
Sbjct: 217 MAGTSRDVGEDVRAAKHMRVGSSRHDNGGFQVDQVALKKSFLGFVKRVFE 266


>At1g35320.1 68414.m04378 expressed protein
          Length = 199

 Score = 27.9 bits (59), Expect = 6.5
 Identities = 11/33 (33%), Positives = 21/33 (63%)
 Frame = +3

Query: 357 YQISSLRVYYPKDLRPSDNRKSVLKTIGEVKKR 455
           +Q+  L++Y+P DL+ SD    V   +G++ +R
Sbjct: 147 FQVEVLKIYFPTDLKESDREFGV---VGKILRR 176


>At3g45590.1 68416.m04924 tRNA-splicing endonuclease, putative
           contains similarity to Swiss-Prot:P16658 tRNA-splicing
           endonuclease subunit SEN2 (tRNA-intron endonuclease)
           [Saccharomyces cerevisiae]; contains Pfam domain
           PF01974: tRNA intron endonuclease, catalytic C-terminal
           domain
          Length = 237

 Score = 27.5 bits (58), Expect = 8.5
 Identities = 9/18 (50%), Positives = 13/18 (72%)
 Frame = +2

Query: 419 ICIEDYRRGEKEVPRWSP 472
           +C+EDY   E+ + RWSP
Sbjct: 210 LCLEDYTVEEQTIRRWSP 227


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,970,386
Number of Sequences: 28952
Number of extensions: 301275
Number of successful extensions: 1013
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 989
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1008
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1412971776
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)