Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0400
(728 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64870.1 68418.m08160 expressed protein 40 0.001
At5g25260.1 68418.m02994 expressed protein 38 0.009
At5g25250.1 68418.m02993 expressed protein 36 0.021
At5g54100.1 68418.m06736 band 7 family protein similar to stomat... 36 0.028
At4g27585.1 68417.m03962 band 7 family protein similar to stomat... 35 0.064
At4g14760.1 68417.m02271 M protein repeat-containing protein con... 31 0.59
At1g79350.1 68414.m09247 DNA-binding protein, putative contains ... 30 1.4
At3g26400.1 68416.m03292 eukaryotic translation initiation facto... 30 1.8
At3g59290.1 68416.m06609 epsin N-terminal homology (ENTH) domain... 29 2.4
At2g43160.3 68415.m05361 epsin N-terminal homology (ENTH) domain... 29 4.2
At2g43160.2 68415.m05360 epsin N-terminal homology (ENTH) domain... 29 4.2
At2g43160.1 68415.m05359 epsin N-terminal homology (ENTH) domain... 29 4.2
At3g23750.1 68416.m02986 leucine-rich repeat family protein / pr... 28 5.5
At2g17120.1 68415.m01976 peptidoglycan-binding LysM domain-conta... 28 5.5
At1g30360.1 68414.m03712 early-responsive to dehydration stress ... 28 5.5
At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing ... 28 7.3
At5g63280.1 68418.m07942 zinc finger (C2H2 type) family protein ... 27 9.6
At5g58880.1 68418.m07377 hypothetical protein 27 9.6
At3g22790.1 68416.m02873 kinase interacting family protein simil... 27 9.6
At1g72100.1 68414.m08334 late embryogenesis abundant domain-cont... 27 9.6
>At5g64870.1 68418.m08160 expressed protein
Length = 479
Score = 40.3 bits (90), Expect = 0.001
Identities = 47/218 (21%), Positives = 87/218 (39%), Gaps = 9/218 (4%)
Frame = +2
Query: 50 NEALIVSGGCFGPTTKRTIVGGWAWAW--CLVTDVQRISLEVMTLNPMCEYVETAQGVPL 223
++ L ++GG G T + W + W C V DV ++ + M +++ +P
Sbjct: 9 SQYLAITGG--GITDIKLAKKSWVFPWQSCTVFDVSPVNY-TFEVQAM-----SSEKLPF 60
Query: 224 TVTGVAQCKIMNEDE---LLTTACEQFLGKTVKEIKMTVLQTLEGHLRAILGTLTVEEVY 394
+ V +D LL K + V +EG R ++ ++T+EEV+
Sbjct: 61 VIPAVFTIGPRVDDPHALLLYAMLMSQHDKHSNHVNELVQGVIEGETRVLVASMTMEEVF 120
Query: 395 KDRDQFAGLVREVAAPDVGRMGIEILSFTIKDVYD--DVQYLASLGKSQTANVKRDADVG 568
K +F V + ++ + G+ I + +K + D +Y + LG+ A +
Sbjct: 121 KGTKEFKKEVFDKVQLELNQFGLVIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQAKID 180
Query: 569 VALANR--DAGIREAECEKNAMDVKYSMDTKIEDNTRL 676
VA A + G +E K ++KI RL
Sbjct: 181 VAEAKMKGEVGAKERTGLTIQNAAKIDAESKIISTQRL 218
>At5g25260.1 68418.m02994 expressed protein
Length = 463
Score = 37.5 bits (83), Expect = 0.009
Identities = 27/132 (20%), Positives = 59/132 (44%), Gaps = 2/132 (1%)
Frame = +2
Query: 302 KTVKEIKMTVLQTLEGHLRAILGTLTVEEVYKDRDQFAGLVREVAAPDVGRMGIEILSFT 481
K + V +EG R + ++T+EE++K +F V + ++ + G+ I +
Sbjct: 89 KQSNHVNELVEGVIEGETRVLAASMTMEEIFKGTKEFKKEVFDKVQLELDQFGLVIYNAN 148
Query: 482 IKDVYD--DVQYLASLGKSQTANVKRDADVGVALANRDAGIREAECEKNAMDVKYSMDTK 655
+K + D +Y + LG+ A + VA A I E+ + ++ + K
Sbjct: 149 VKQLVDVPGHEYFSYLGQKTQMEAANQARIDVAEAKMKGEI--GAKERTGLTLQNA--AK 204
Query: 656 IEDNTRLFKLQK 691
I+ +++ +Q+
Sbjct: 205 IDAESKIISMQR 216
>At5g25250.1 68418.m02993 expressed protein
Length = 470
Score = 36.3 bits (80), Expect = 0.021
Identities = 24/119 (20%), Positives = 56/119 (47%), Gaps = 2/119 (1%)
Frame = +2
Query: 341 LEGHLRAILGTLTVEEVYKDRDQFAGLVREVAAPDVGRMGIEILSFTIKDVYD--DVQYL 514
+EG R + ++T+EE++K +F V + ++ + G+ I + +K + D +Y
Sbjct: 102 IEGETRVLAASMTMEEIFKGTKEFKKEVFDKVQLELNQFGLVIYNANVKQLVDVPGHEYF 161
Query: 515 ASLGKSQTANVKRDADVGVALANRDAGIREAECEKNAMDVKYSMDTKIEDNTRLFKLQK 691
+ LG+ A + V+ A I E+ + ++ + KI+ +++ +Q+
Sbjct: 162 SYLGQKTQMEAANQARIDVSEAKMKGEI--GAKERTGLTLQNA--AKIDAESKIISMQR 216
>At5g54100.1 68418.m06736 band 7 family protein similar to
stomatin-like protein [Zea mays] GI:7716464; contains
Pfam profile PF01145: SPFH domain / Band 7 family
Length = 401
Score = 35.9 bits (79), Expect = 0.028
Identities = 21/100 (21%), Positives = 48/100 (48%)
Frame = +2
Query: 329 VLQTLEGHLRAILGTLTVEEVYKDRDQFAGLVREVAAPDVGRMGIEILSFTIKDVYDDVQ 508
V+Q + +R+ LG +T+++ +++RD + E G++ L + I+D+
Sbjct: 194 VMQLAQTTMRSELGKITLDKTFEERDTLNEKIVEAINVAAKDWGLQCLRYEIRDIMPPNG 253
Query: 509 YLASLGKSQTANVKRDADVGVALANRDAGIREAECEKNAM 628
++ A K+ A + + R A I A+ +K+++
Sbjct: 254 VRVAMEMQAEAERKKRAQILESEGERQAHINRADGKKSSV 293
>At4g27585.1 68417.m03962 band 7 family protein similar to
stomatin-like protein [Zea mays] GI:7716464; contains
Pfam profile PF01145: SPFH domain / Band 7 family
Length = 411
Score = 34.7 bits (76), Expect = 0.064
Identities = 21/100 (21%), Positives = 49/100 (49%)
Frame = +2
Query: 329 VLQTLEGHLRAILGTLTVEEVYKDRDQFAGLVREVAAPDVGRMGIEILSFTIKDVYDDVQ 508
V+Q + +R+ LG +T+++ +++RD + E G++ L + I+D+
Sbjct: 151 VVQLAQTTMRSELGKITLDKTFEERDTLNEKIVEAINVAAKDWGLQCLRYEIRDIMPPHG 210
Query: 509 YLASLGKSQTANVKRDADVGVALANRDAGIREAECEKNAM 628
A++ A K+ A + + R + I A+ +K+++
Sbjct: 211 VRAAMEMQAEAERKKRAQILESEGERQSHINIADGKKSSV 250
>At4g14760.1 68417.m02271 M protein repeat-containing protein
contains Pfam profile: PF02370 M protein repeat
Length = 1676
Score = 31.5 bits (68), Expect = 0.59
Identities = 16/71 (22%), Positives = 31/71 (43%)
Frame = +2
Query: 497 DDVQYLASLGKSQTANVKRDADVGVALANRDAGIREAECEKNAMDVKYSMDTKIEDNTRL 676
+++Q + K ++AN KR + +A ++ EC + + +D + +
Sbjct: 731 EELQVSLAAEKQESANYKRSTESRLADLQKNVSFLREECRSRKREYEDELDRVVNKQVEI 790
Query: 677 FKLQKAQFDQE 709
F LQK D E
Sbjct: 791 FILQKLIEDLE 801
>At1g79350.1 68414.m09247 DNA-binding protein, putative contains
Pfam PF00628: PHD-finger domain; contains TIGRFAMS
TIGR01053: zinc finger domain, LSD1 subclass; contains
Pfam PF00271: Helicase conserved C-terminal domain;
similar to WSSV086 (GI:19481678)[shrimp white spot
syndrome virus]; similar to nuclear protein Np95
(GI:17939938) [Mus musculus]
Length = 1299
Score = 30.3 bits (65), Expect = 1.4
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -3
Query: 144 SVTRHQAHAHPPTMVRFVVGPKQPPETIS 58
S+T Q+H++PPT V P PPE ++
Sbjct: 152 SLTASQSHSNPPTPAAPTVPPPPPPEEVN 180
>At3g26400.1 68416.m03292 eukaryotic translation initiation factor
4B, putative/ eIF-4B, putative similar to eukaryotic
initiation factor 4B [Arabidopsis thaliana] GI:6739518
Length = 532
Score = 29.9 bits (64), Expect = 1.8
Identities = 26/90 (28%), Positives = 36/90 (40%), Gaps = 3/90 (3%)
Frame = +1
Query: 292 VPGQDRQRD*DDC-PANVGGSFASYSRNSYSRRGVQRQGPIRGLGTRSGCAGRRKNGN-- 462
+P RQR ++ P +GG F+SY S R G R G GRR G
Sbjct: 89 LPTGPRQRSEEEMQPGRLGGGFSSYGGRSGGRIGRDRDDSDGSWSGGGGGGGRRPYGGGF 148
Query: 463 RDSVLHD*GRLRRCPVSGQLGEVADGQREE 552
D + R+ P + EV D +E+
Sbjct: 149 DDDRRGNQSRVSDFPQPSRADEVDDWGKEK 178
>At3g59290.1 68416.m06609 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain;
similar to Af10-protein - Avena fatua, EMBL:U80041
Length = 1024
Score = 29.5 bits (63), Expect = 2.4
Identities = 18/45 (40%), Positives = 23/45 (51%)
Frame = +2
Query: 506 QYLASLGKSQTANVKRDADVGVALANRDAGIREAECEKNAMDVKY 640
QY+ S GK Q +NV++ A VAL N I E + A KY
Sbjct: 116 QYIDSSGKDQGSNVRKKAQSLVALVNDKERITEVREKAAANRDKY 160
>At2g43160.3 68415.m05361 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Af10-protein (GI:1724114) [Avena fatua]
Length = 895
Score = 28.7 bits (61), Expect = 4.2
Identities = 17/47 (36%), Positives = 24/47 (51%)
Frame = +2
Query: 500 DVQYLASLGKSQTANVKRDADVGVALANRDAGIREAECEKNAMDVKY 640
D QY+ S G+ Q +NV++ + VAL N I E + A KY
Sbjct: 114 DFQYIDSGGRDQGSNVRKKSQSLVALVNDKERIAEVRQKAAANRDKY 160
>At2g43160.2 68415.m05360 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Af10-protein (GI:1724114) [Avena fatua]
Length = 895
Score = 28.7 bits (61), Expect = 4.2
Identities = 17/47 (36%), Positives = 24/47 (51%)
Frame = +2
Query: 500 DVQYLASLGKSQTANVKRDADVGVALANRDAGIREAECEKNAMDVKY 640
D QY+ S G+ Q +NV++ + VAL N I E + A KY
Sbjct: 114 DFQYIDSGGRDQGSNVRKKSQSLVALVNDKERIAEVRQKAAANRDKY 160
>At2g43160.1 68415.m05359 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Af10-protein (GI:1724114) [Avena fatua]
Length = 895
Score = 28.7 bits (61), Expect = 4.2
Identities = 17/47 (36%), Positives = 24/47 (51%)
Frame = +2
Query: 500 DVQYLASLGKSQTANVKRDADVGVALANRDAGIREAECEKNAMDVKY 640
D QY+ S G+ Q +NV++ + VAL N I E + A KY
Sbjct: 114 DFQYIDSGGRDQGSNVRKKSQSLVALVNDKERIAEVRQKAAANRDKY 160
>At3g23750.1 68416.m02986 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 928
Score = 28.3 bits (60), Expect = 5.5
Identities = 21/59 (35%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = +2
Query: 488 DVYDDVQYLASLGKSQTANVKRDADVGVALANRDAGIREAE--CEKNAMDVKYSMDTKI 658
DV V+YL SL +Q + + RD L D + A+ KNA D KYS++T++
Sbjct: 687 DVARGVEYLHSL--AQQSFIHRDLKPSNILLGDDMRAKVADFGLVKNAPDGKYSVETRL 743
>At2g17120.1 68415.m01976 peptidoglycan-binding LysM
domain-containing protein contains Pfam profile PF01476:
LysM domain; supporting cDNA
gi|16226688|gb|AF428464.1|AF428464
Length = 350
Score = 28.3 bits (60), Expect = 5.5
Identities = 19/81 (23%), Positives = 34/81 (41%)
Frame = -3
Query: 411 NWSLSLYTSSTVRVPRIARK*PSNVCRTVILISLTVLPRNCSQAVVRSSSSFMILHWATP 232
NW+LS +SS ++ P + C +L + PR+C A + + F A P
Sbjct: 258 NWTLSCQSSSEIKPSNWQTCPPFSQCDGALLNASCRQPRDCVYAGYSNQTIFTTASPACP 317
Query: 231 VTVSGTPWAVSTYSHIGFNVI 169
+ +A + S F ++
Sbjct: 318 DSAGPDNYASTLSSSFNFVIV 338
>At1g30360.1 68414.m03712 early-responsive to dehydration stress
protein (ERD4) nearly identical to ERD4 protein
(early-responsive to dehydration stress) [Arabidopsis
thaliana] GI:15375406; contains Pfam profile PF02714:
Domain of unknown function DUF221
Length = 724
Score = 28.3 bits (60), Expect = 5.5
Identities = 15/50 (30%), Positives = 28/50 (56%)
Frame = +2
Query: 404 DQFAGLVREVAAPDVGRMGIEILSFTIKDVYDDVQYLASLGKSQTANVKR 553
+QFA LVR++ AP G+ E + +++Y + Y SL ++ + V +
Sbjct: 190 EQFAILVRDMPAPPDGQTQKEFIDSYFREIYPETFY-RSLVATENSKVNK 238
>At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing
protein low similarity to nucleolar phosphoprotein
(Nopp52), Tetrahymena thermophila, EMBL:TT51555;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 597
Score = 27.9 bits (59), Expect = 7.3
Identities = 14/51 (27%), Positives = 24/51 (47%)
Frame = -3
Query: 480 VKDRISIPILPTSGAATSRTKPANWSLSLYTSSTVRVPRIARK*PSNVCRT 328
+K RI + + + S T P + SL L S+ PR++++ C T
Sbjct: 8 LKQRIRQDLAESLSVSVSETNPQSQSLKLLLDSSSHKPRLSKREKRRNCET 58
>At5g63280.1 68418.m07942 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 271
Score = 27.5 bits (58), Expect = 9.6
Identities = 25/98 (25%), Positives = 40/98 (40%), Gaps = 6/98 (6%)
Frame = -2
Query: 550 LHVGRLRLPQAGQIL-DIVVDVLNREGQNLDSHSSDVRRSHFAYQARELVPVFVHLF--- 383
+H R R A QI+ D + + RE + + + + Y+ +E V V +F
Sbjct: 48 IHCSRERSRAAWQIIQDYLTPFVERERYEIPKNCR-LHPDNDLYRDQEHHKVHVDVFEWK 106
Query: 382 --YCKSSENSSQMTLQRLQDSHLNLFDGLAQELLAGSC 275
YCK S N + + H NL + + LA C
Sbjct: 107 CGYCKKSFNDEKFLDKHFSTRHYNLLNTTDTKCLADLC 144
>At5g58880.1 68418.m07377 hypothetical protein
Length = 1088
Score = 27.5 bits (58), Expect = 9.6
Identities = 15/47 (31%), Positives = 26/47 (55%)
Frame = -2
Query: 373 SSENSSQMTLQRLQDSHLNLFDGLAQELLAGSCEKFVFVHDLALGDS 233
++ENSS + L ++QDSH + D + ++ EK + D G+S
Sbjct: 832 NAENSSDVILLQVQDSHKSPLDESVDQEISKEVEKTELLKDFC-GES 877
>At3g22790.1 68416.m02873 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 1694
Score = 27.5 bits (58), Expect = 9.6
Identities = 18/62 (29%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
Frame = +2
Query: 527 KSQTANVKRDADVGVA-LANRDAGIREAECEKNAMDVKYSMDTKIEDNTRLFKLQKAQFD 703
K + A+ +R D +A L N + +RE EC + + +D + +F LQK D
Sbjct: 778 KQERASYERSTDTRLADLQNNVSFLRE-ECRSRKKEFEEELDRAVNAQVEIFILQKFIED 836
Query: 704 QE 709
E
Sbjct: 837 LE 838
>At1g72100.1 68414.m08334 late embryogenesis abundant
domain-containing protein / LEA domain-containing
protein low similarity to embryogenic gene [Betula
pendula] GI:4539485; contains Pfam profile PF02987: Late
embryogenesis abundant protein
Length = 480
Score = 27.5 bits (58), Expect = 9.6
Identities = 26/81 (32%), Positives = 35/81 (43%)
Frame = +2
Query: 422 VREVAAPDVGRMGIEILSFTIKDVYDDVQYLASLGKSQTANVKRDADVGVALANRDAGIR 601
VRE AA + E ++ +DV D V A K + D GVA DA +
Sbjct: 160 VREAAAHKAHDVK-ETVTHAARDVEDTVADQAQYAKGRVTEKAHDPKEGVAHKAHDA--K 216
Query: 602 EAECEKNAMDVKYSMDTKIED 664
E+ +K A D K S+ K D
Sbjct: 217 ESVADK-AHDAKESVAQKAHD 236
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,110,420
Number of Sequences: 28952
Number of extensions: 393035
Number of successful extensions: 1142
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1142
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -