BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0386 (698 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g03410.2 68417.m00465 peroxisomal membrane protein-related co... 29 2.2 At4g03410.1 68417.m00464 peroxisomal membrane protein-related co... 29 2.2 At1g79730.1 68414.m09300 hydroxyproline-rich glycoprotein family... 29 2.2 At1g69545.1 68414.m07997 leucine-rich repeat family protein cont... 29 3.0 At5g35690.1 68418.m04267 expressed protein 29 3.9 At5g08500.1 68418.m01007 transmembrane CLPTM1 family protein con... 29 3.9 At1g75290.1 68414.m08746 isoflavone reductase, putative similar ... 29 3.9 At5g18840.1 68418.m02239 sugar transporter, putative similar to ... 27 9.0 At4g01830.1 68417.m00240 multidrug resistance P-glycoprotein, pu... 27 9.0 >At4g03410.2 68417.m00465 peroxisomal membrane protein-related contains weak similarity to Swiss-Prot:P42925 22 kDa peroxisomal membrane protein [Mus musculus] Length = 361 Score = 29.5 bits (63), Expect = 2.2 Identities = 22/76 (28%), Positives = 38/76 (50%), Gaps = 3/76 (3%) Frame = +2 Query: 293 IVHDKFEE-TKYEV--FSPEELWKQFLHVRLNTVLPLRCEATISGVKNSLQNKRKKIASG 463 I HD ++ T YE+ ++PE W F + ++ P+ + ISG+ SL + + G Sbjct: 106 IDHDYWQGWTLYEILRYAPEHNW--FAYEQILKTNPVLAKMAISGIVYSLGDWIAQCYEG 163 Query: 464 QVSFHIDSTQVYLFGV 511 + F D T+V G+ Sbjct: 164 KPLFEFDRTRVLRSGL 179 >At4g03410.1 68417.m00464 peroxisomal membrane protein-related contains weak similarity to Swiss-Prot:P42925 22 kDa peroxisomal membrane protein [Mus musculus] Length = 317 Score = 29.5 bits (63), Expect = 2.2 Identities = 22/76 (28%), Positives = 38/76 (50%), Gaps = 3/76 (3%) Frame = +2 Query: 293 IVHDKFEE-TKYEV--FSPEELWKQFLHVRLNTVLPLRCEATISGVKNSLQNKRKKIASG 463 I HD ++ T YE+ ++PE W F + ++ P+ + ISG+ SL + + G Sbjct: 106 IDHDYWQGWTLYEILRYAPEHNW--FAYEQILKTNPVLAKMAISGIVYSLGDWIAQCYEG 163 Query: 464 QVSFHIDSTQVYLFGV 511 + F D T+V G+ Sbjct: 164 KPLFEFDRTRVLRSGL 179 >At1g79730.1 68414.m09300 hydroxyproline-rich glycoprotein family protein contains proline-rich extensin domains, INTERPRO:IPR002965 Length = 589 Score = 29.5 bits (63), Expect = 2.2 Identities = 15/39 (38%), Positives = 22/39 (56%) Frame = +2 Query: 278 MKAHFIVHDKFEETKYEVFSPEELWKQFLHVRLNTVLPL 394 +K I DK + TKY + S E+LWK + V + +PL Sbjct: 211 LKLMTIKRDKDQFTKYTITSLEKLWKPKIFVEPDLGIPL 249 >At1g69545.1 68414.m07997 leucine-rich repeat family protein contains Pfam PF00560: Leucine Rich Repeat domains; similar to disease resistance protein RPP1-WsA (GI:3860163)[Arabidopsis thaliana] Length = 703 Score = 29.1 bits (62), Expect = 3.0 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = -3 Query: 648 IKLTGNISALLILYFFFFDGCSGLIESTSS 559 ++L +I L+ L F+F GCS L+E SS Sbjct: 105 VELPSSIGNLINLEAFYFHGCSSLLELPSS 134 >At5g35690.1 68418.m04267 expressed protein Length = 603 Score = 28.7 bits (61), Expect = 3.9 Identities = 11/25 (44%), Positives = 18/25 (72%) Frame = +2 Query: 131 LLNFPAEIELCGVVKFGETLTTDST 205 +LNFPA +E+ VV F + + ++ST Sbjct: 547 ILNFPAAVEILSVVGFVDEMVSEST 571 >At5g08500.1 68418.m01007 transmembrane CLPTM1 family protein contains Pfam profile PF05602: Cleft lip and palate transmembrane protein 1 (CLPTM1) Length = 590 Score = 28.7 bits (61), Expect = 3.9 Identities = 16/43 (37%), Positives = 24/43 (55%) Frame = +2 Query: 44 LMYDGTLLVVGFSLESFETETEKNSYSQLLLNFPAEIELCGVV 172 L Y LLVVGFS+ S E K+ YS +L + + + + G + Sbjct: 447 LSYVLLLLVVGFSIYSLAYERHKSWYSWILSSLTSCVYMFGFI 489 >At1g75290.1 68414.m08746 isoflavone reductase, putative similar to SP|P52577 Isoflavone reductase homolog P3 (EC 1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile PF02716: Isoflavone reductase Length = 323 Score = 28.7 bits (61), Expect = 3.9 Identities = 20/68 (29%), Positives = 35/68 (51%), Gaps = 1/68 (1%) Frame = +2 Query: 95 ETETEKNSYSQLLLNFPAEIELCGVVKFGETLTTDSTTK-EILQDVDITDNPLIIIINRE 271 + E E Y+ L+ N+ A L +V+ LT+ K +I D ++ I +IN+E Sbjct: 140 DIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIFGDGNVKVEYFIAVINKE 199 Query: 272 KDMKAHFI 295 +D+ A+ I Sbjct: 200 EDIAAYTI 207 >At5g18840.1 68418.m02239 sugar transporter, putative similar to ERD6 protein {Arabidopsis thaliana} GI:3123712, sugar-porter family protein 1 [Arabidopsis thaliana] GI:14585699; contains Pfam profile PF00083: major facilitator superfamily protein Length = 482 Score = 27.5 bits (58), Expect = 9.0 Identities = 16/52 (30%), Positives = 29/52 (55%), Gaps = 1/52 (1%) Frame = +2 Query: 86 ESFETETEKNSYSQLLLNFPAEIELCGVVKFGETLTTDSTTK-EILQDVDIT 238 E E E+E N S L++ F + +CG +FG + + T+ I QD++++ Sbjct: 29 EDDEKESENNE-SYLMVLFSTFVAVCGSFEFGSCVGYSAPTQSSIRQDLNLS 79 >At4g01830.1 68417.m00240 multidrug resistance P-glycoprotein, putative similar to multidrug resistant P-glycoprotein GI:4204793 from [Solanum tuberosum] Length = 1230 Score = 27.5 bits (58), Expect = 9.0 Identities = 19/64 (29%), Positives = 30/64 (46%), Gaps = 4/64 (6%) Frame = +2 Query: 422 KNSLQNKRKKIASGQVSFHIDSTQVYLFGVAS----DVGLTGTSTEATVGELVDSMSPEQ 589 K +L+ K + ++ F DST V L V S G+ GEL+D+M P Q Sbjct: 3 KGNLEANTKTVPFYKLFFFSDSTDVLLMIVGSIGAIANGVCSPLMTLLFGELIDAMGPNQ 62 Query: 590 PSKK 601 +++ Sbjct: 63 NNEE 66 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,427,851 Number of Sequences: 28952 Number of extensions: 287185 Number of successful extensions: 805 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 783 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 805 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1496852856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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