BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0374 (746 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom... 38 0.002 SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 31 0.13 SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 29 0.93 SPAC18B11.11 ||SPAC1F5.01|GTPase activating protein |Schizosacch... 29 0.93 SPBC1773.14 |arg7||argininosuccinate lyase |Schizosaccharomyces ... 25 8.7 SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 25 8.7 SPBC3H7.06c |pof9||F-box protein Paf9|Schizosaccharomyces pombe|... 25 8.7 >SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pombe|chr 2|||Manual Length = 396 Score = 37.5 bits (83), Expect = 0.002 Identities = 37/179 (20%), Positives = 80/179 (44%), Gaps = 5/179 (2%) Frame = +2 Query: 101 NAVSNPTKVKMEESPALLNLPAEMIILIIKYLDTPTVYNIYNTCKPLREIITQGVVKNLK 280 N +P + ++ S L L ++ LII YLD + + ++ + + + V Sbjct: 29 NHEEDPIQPVLKNSNLFL-LNRDIWSLIINYLDAFDILRLMHSSRQFYYWLRKSAVDECC 87 Query: 281 FSLNILSTADSLKTDFFKAVAYSLKDLSLSGMHDLTKTKLIQA-----VRHLKSLQTLDV 445 F+ N L+ +T + ++ L G + K +L + + + LQT+ + Sbjct: 88 FNNNFLNLQPYQRTVPVASDLEWATEVDLYGNPPILKLQLRDSFVWSMLAKFQGLQTIAL 147 Query: 446 TYTDISICDLLDIHPLCPTVKNIAINYIFGKLGMTRMTPEFIEACQNLLAKFENIHFVG 622 T ++I + +I PTVK ++I + G ++ + EF++ ++ EN++ +G Sbjct: 148 DGTGVTISSVTNILLNIPTVKTLSIRWCVGVCSLSLI--EFLQNSKSRTFSLENLYVLG 204 >SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pombe|chr 2|||Manual Length = 662 Score = 31.5 bits (68), Expect = 0.13 Identities = 12/36 (33%), Positives = 25/36 (69%) Frame = +2 Query: 149 LLNLPAEMIILIIKYLDTPTVYNIYNTCKPLREIIT 256 +LNLP E++I+I +LD ++ + TCK +++++ Sbjct: 31 VLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLS 66 >SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual Length = 4717 Score = 28.7 bits (61), Expect = 0.93 Identities = 22/74 (29%), Positives = 32/74 (43%), Gaps = 4/74 (5%) Frame = -3 Query: 465 MLISVYVTSKVCRLFKWRTACMSLVFVKSCIPLSDRSFRL*ATALKKSVFKE----SAVD 298 +L S+ +K R FK S + + SC R+F L ++K S KE S+VD Sbjct: 3779 LLSSIETATKNMRTFKELAETSSFIEMSSCFSKVLRAFNLKFQSMKLSSLKEKLRSSSVD 3838 Query: 297 SMFRENFKFFTTPC 256 M + F C Sbjct: 3839 KMSCQLLMLFLPVC 3852 >SPAC18B11.11 ||SPAC1F5.01|GTPase activating protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1294 Score = 28.7 bits (61), Expect = 0.93 Identities = 25/100 (25%), Positives = 46/100 (46%) Frame = +2 Query: 353 KDLSLSGMHDLTKTKLIQAVRHLKSLQTLDVTYTDISICDLLDIHPLCPTVKNIAINYIF 532 +DLS S + K+ + K L +L + ++ ++ V+ I IN+ Sbjct: 648 RDLSFSPVFQKFPLKVNTSATLSKDLMSLPINEWVKNVMYIVTHETDWKVVQYILINFT- 706 Query: 533 GKLGMTRMTPEFIEACQNLLAKFENIHFVGSSINLMYSNF 652 +L T+M + +EA Q LL +I S+N+ +S+F Sbjct: 707 NQLRNTKMFTKTVEALQLLLNSLRDIINGKQSLNINFSDF 746 >SPBC1773.14 |arg7||argininosuccinate lyase |Schizosaccharomyces pombe|chr 2|||Manual Length = 461 Score = 25.4 bits (53), Expect = 8.7 Identities = 29/127 (22%), Positives = 54/127 (42%), Gaps = 7/127 (5%) Frame = +2 Query: 167 EMIILIIKYLDTPTVYN--IYNTCKPLREIITQGVVKNLKFSLNILSTADSLKTDFFKAV 340 +MI L+I TPT YN + +PL + + V +L+ ++ST T +++ Sbjct: 307 DMIGLMITVKGTPTTYNKDLQEDKEPLFDAF-KTVSDSLQILTGVVSTLTINPTKIAESL 365 Query: 341 AYSLKDLSLSGMHDLTKTKLIQAVRHL-----KSLQTLDVTYTDISICDLLDIHPLCPTV 505 L L+ + + K + H+ + + + T +S+ DL +HPL Sbjct: 366 TPDLLATDLA-EYLVRKGLPFRQTHHISGSAVRMAEERNTTLDKLSVSDLQSLHPLFDED 424 Query: 506 KNIAINY 526 + NY Sbjct: 425 VSKVFNY 431 >SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccharomyces pombe|chr 1|||Manual Length = 569 Score = 25.4 bits (53), Expect = 8.7 Identities = 23/90 (25%), Positives = 34/90 (37%), Gaps = 3/90 (3%) Frame = +2 Query: 113 NPTKVKMEESPALLNLPAEMIILIIKYLDTPTVYNIYNTCKPLREIITQG--VVKNLKF- 283 NP + SP + P+ + L + YN T P+ Q N F Sbjct: 301 NPLSISSRPSPCCPSTPSSAVTLSNGFDSQSNAYNNSGTGPPMLYKFPQRSYTAPNTSFN 360 Query: 284 SLNILSTADSLKTDFFKAVAYSLKDLSLSG 373 S +S+ SL T F V + + SL+G Sbjct: 361 SQRRMSSITSLPTASFCPVKHGVSPPSLAG 390 >SPBC3H7.06c |pof9||F-box protein Paf9|Schizosaccharomyces pombe|chr 2|||Manual Length = 467 Score = 25.4 bits (53), Expect = 8.7 Identities = 13/37 (35%), Positives = 21/37 (56%) Frame = +2 Query: 131 MEESPALLNLPAEMIILIIKYLDTPTVYNIYNTCKPL 241 M +SP L L ++++ I YLD + ++ TCK L Sbjct: 1 MAKSP-FLELSYDILLEISTYLDYKDIVHLSETCKSL 36 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,012,305 Number of Sequences: 5004 Number of extensions: 63027 Number of successful extensions: 135 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 130 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 135 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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