BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0369 (677 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g80600.1 68414.m09457 acetylornithine aminotransferase, mitoc... 31 0.93 At1g63700.1 68414.m07209 protein kinase, putative contains prote... 31 0.93 At1g68460.1 68414.m07821 adenylate isopentenyltransferase 1 / cy... 30 1.6 At5g46875.1 68418.m05776 hypothetical protein 28 4.9 At5g19560.1 68418.m02329 hypothetical protein contains Pfam prof... 28 4.9 At3g11325.1 68416.m01377 hypothetical protein 27 8.6 >At1g80600.1 68414.m09457 acetylornithine aminotransferase, mitochondrial, putative / acetylornithine transaminase, putative / AOTA, putative / ACOAT, putative similar to SP|O04866 Acetylornithine aminotransferase, mitochondrial precursor (EC 2.6.1.11) (ACOAT) (Acetylornithine transaminase) (AOTA) {Alnus glutinosa}; contains Pfam profile PF00202: aminotransferase, class III Length = 457 Score = 30.7 bits (66), Expect = 0.93 Identities = 13/16 (81%), Positives = 14/16 (87%) Frame = +3 Query: 231 GTYSRAKDVLSSGKGC 278 GTY+RA VLSSGKGC Sbjct: 71 GTYARAPVVLSSGKGC 86 >At1g63700.1 68414.m07209 protein kinase, putative contains protein kinase domain, Pfam:PF00069; similar to MEK kinase (MAP3Ka) [Arabidopsis thaliana] gi|4204912|gb|AAD10848 Length = 883 Score = 30.7 bits (66), Expect = 0.93 Identities = 15/33 (45%), Positives = 20/33 (60%), Gaps = 1/33 (3%) Frame = +3 Query: 462 SEGRSRSTNRKRSHIAG-RWSVGRRTRRSPSPN 557 S GRSR + R+R I R ++ R RSPSP+ Sbjct: 35 SSGRSRKSRRRRDEIVSERGAISRLPSRSPSPS 67 >At1g68460.1 68414.m07821 adenylate isopentenyltransferase 1 / cytokinin synthase (IPT1) identical to adenylate isopentenyltransferase (IPT1) [Arabidopsis thaliana] GI:14279054 Length = 357 Score = 29.9 bits (64), Expect = 1.6 Identities = 22/68 (32%), Positives = 32/68 (47%), Gaps = 8/68 (11%) Frame = +1 Query: 313 GLQIAIPVDEFES--SVIKPEKKFVKWYAA------PAEGEPSRDYWTATQYYVSEESLK 468 G++ AI V EF+ PEKK +KW A A + R+ WT + V + + Sbjct: 246 GIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEML 305 Query: 469 AGAGPQIE 492 AG +IE Sbjct: 306 KDAGWEIE 313 >At5g46875.1 68418.m05776 hypothetical protein Length = 132 Score = 28.3 bits (60), Expect = 4.9 Identities = 16/53 (30%), Positives = 22/53 (41%) Frame = +1 Query: 478 GPQIENGATLQDGGVWVAGPDAHLLRIPTTEAELNSTFFKKQNCIPNMGTHYY 636 GP + G G W G D H+ R AEL F K + +M + +Y Sbjct: 55 GPTMAGGVLQDSKGRWCDGFDVHIGRCSAPLAELWGGFLKTRTDDSHMMSSWY 107 >At5g19560.1 68418.m02329 hypothetical protein contains Pfam profile PF03759: Domain of unknown function (DUF315) Length = 493 Score = 28.3 bits (60), Expect = 4.9 Identities = 12/29 (41%), Positives = 20/29 (68%), Gaps = 1/29 (3%) Frame = +1 Query: 139 DALADIEYFYSIPRTIKDAENDG-WRRTE 222 D D + F+ +PR ++DA+++G WRR E Sbjct: 163 DNFKDQKDFWYVPRDMEDADHNGDWRRDE 191 >At3g11325.1 68416.m01377 hypothetical protein Length = 401 Score = 27.5 bits (58), Expect = 8.6 Identities = 14/42 (33%), Positives = 21/42 (50%) Frame = +1 Query: 199 NDGWRRTEPPPGPILELRMYCPPGRVVCPLYDTSGFVAGLQI 324 NDG P P L + ++ P G V+ P+ SGF+ + I Sbjct: 145 NDGRLVKRPTPATALLILIWIPFGMVLSPIRILSGFILPMWI 186 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.136 0.424 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,315,513 Number of Sequences: 28952 Number of extensions: 387624 Number of successful extensions: 1091 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 1040 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1090 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1428369392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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