BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0366 (507 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosa... 154 6e-39 SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Sch... 153 1e-38 SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 32 0.057 SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyce... 31 0.099 SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p... 27 1.6 SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub... 25 4.9 SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 25 6.5 SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 25 6.5 SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 25 8.6 SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 25 8.6 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 25 8.6 SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd... 25 8.6 >SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosaccharomyces pombe|chr 2|||Manual Length = 148 Score = 154 bits (374), Expect = 6e-39 Identities = 70/113 (61%), Positives = 88/113 (77%) Frame = +1 Query: 79 MDVNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNE 258 + V +L+EVLK AL+H GL G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E Sbjct: 30 LSVEDSLKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAE 89 Query: 259 HQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 417 Q PLVKV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL L Sbjct: 90 SQTPLVKVADPKILGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSVALQTL 142 >SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Schizosaccharomyces pombe|chr 3|||Manual Length = 145 Score = 153 bits (372), Expect = 1e-38 Identities = 69/113 (61%), Positives = 88/113 (77%) Frame = +1 Query: 79 MDVNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNE 258 + V AL+EVLK AL+H GL G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E Sbjct: 27 VSVEDALKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAE 86 Query: 259 HQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 417 + PL+KV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL L Sbjct: 87 SETPLIKVADPKVLGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSAALQKL 139 >SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 154 Score = 31.9 bits (69), Expect = 0.057 Identities = 17/74 (22%), Positives = 35/74 (47%) Frame = +1 Query: 85 VNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQ 264 +N + + +K A ++ G+ E KA+ K + L +LA + + LC ++ Sbjct: 37 LNKKMMKTVKKASKQKHILRGVKEVVKAVRKGEKGLVILAGDISPMDVISHIPVLCEDNN 96 Query: 265 IPLVKVDNNKKLGE 306 +P + + + LGE Sbjct: 97 VPYLYTVSKELLGE 110 >SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 31.1 bits (67), Expect = 0.099 Identities = 14/54 (25%), Positives = 27/54 (50%) Frame = +1 Query: 142 HGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLG 303 +GL+ ++ ++A L ++A + D + ALC + +P V N +LG Sbjct: 134 YGLNHVVALIEAKKAKLVLIASDVDPIELVVFLPALCKKMGVPYAIVKNKARLG 187 >SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 404 Score = 27.1 bits (57), Expect = 1.6 Identities = 10/25 (40%), Positives = 14/25 (56%) Frame = -1 Query: 405 RWSFLAEIFDDNTGAADNFPCLAIL 331 RW +LA +F N A N P + +L Sbjct: 257 RWEYLASLFTSNFTAVHNIPSVPLL 281 >SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ubp21|Schizosaccharomyces pombe|chr 2|||Manual Length = 1129 Score = 25.4 bits (53), Expect = 4.9 Identities = 14/38 (36%), Positives = 20/38 (52%) Frame = +1 Query: 40 VEVPTNPILSGNNMDVNVALQEVLKTALIHGGLVHGLH 153 +E+ P LS D + + VL L+HGG +HG H Sbjct: 445 LEIDLEPYLS-ETADKSESHVYVLHGVLVHGGDLHGGH 481 >SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr 3|||Manual Length = 923 Score = 25.0 bits (52), Expect = 6.5 Identities = 10/35 (28%), Positives = 19/35 (54%) Frame = +2 Query: 200 WLRTVMKPHTRNLFRLCATNIRFHWSRLTTTKSLE 304 ++ +V P L+RLC++ R W ++SL+ Sbjct: 220 FILSVFHPDAARLYRLCSSLKRICWELYDPSRSLD 254 >SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccharomyces pombe|chr 1|||Manual Length = 549 Score = 25.0 bits (52), Expect = 6.5 Identities = 10/28 (35%), Positives = 20/28 (71%), Gaps = 1/28 (3%) Frame = -3 Query: 373 QHRSSRQFSLPCHPCQSCRD-QPILQAF 293 + ++ +QF LP PCQ+ + +PI+++F Sbjct: 226 KRKALKQFPLPPDPCQALANLRPIVKSF 253 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 24.6 bits (51), Expect = 8.6 Identities = 15/55 (27%), Positives = 24/55 (43%) Frame = -3 Query: 229 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 65 C+ S +HIV A L +D +DH G+ L ++P V + P+ Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571 >SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1133 Score = 24.6 bits (51), Expect = 8.6 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = -3 Query: 136 DHHGLKLSLAPLAVLHSRPC 77 D GL ++ L+++HSRPC Sbjct: 529 DEMGLGKTIEVLSLIHSRPC 548 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 24.6 bits (51), Expect = 8.6 Identities = 15/55 (27%), Positives = 24/55 (43%) Frame = -3 Query: 229 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 65 C+ S +HIV A L +D +DH G+ L ++P V + P+ Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571 >SPBC2G2.08 |ade9||C-1- tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr ogenase/methylenetetrahydrofolatecyclohydrolase/formylte trahydrofolatesynthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 969 Score = 24.6 bits (51), Expect = 8.6 Identities = 11/20 (55%), Positives = 15/20 (75%) Frame = +2 Query: 347 GKLSAAPVLSSKISARKLQR 406 G S+A +L+ K+ ARKLQR Sbjct: 28 GTSSSANILNGKLLARKLQR 47 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,987,601 Number of Sequences: 5004 Number of extensions: 38349 Number of successful extensions: 145 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 143 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 145 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 202220600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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