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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0366
         (507 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosa...   154   6e-39
SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Sch...   153   1e-38
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce...    32   0.057
SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyce...    31   0.099
SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p...    27   1.6  
SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub...    25   4.9  
SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch...    25   6.5  
SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha...    25   6.5  
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...    25   8.6  
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch...    25   8.6  
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...    25   8.6  
SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd...    25   8.6  

>SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein
           S12|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 148

 Score =  154 bits (374), Expect = 6e-39
 Identities = 70/113 (61%), Positives = 88/113 (77%)
 Frame = +1

Query: 79  MDVNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNE 258
           + V  +L+EVLK AL+H GL  G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E
Sbjct: 30  LSVEDSLKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAE 89

Query: 259 HQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 417
            Q PLVKV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL  L
Sbjct: 90  SQTPLVKVADPKILGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSVALQTL 142


>SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein
           S12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 145

 Score =  153 bits (372), Expect = 1e-38
 Identities = 69/113 (61%), Positives = 88/113 (77%)
 Frame = +1

Query: 79  MDVNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNE 258
           + V  AL+EVLK AL+H GL  G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E
Sbjct: 27  VSVEDALKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAE 86

Query: 259 HQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 417
            + PL+KV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL  L
Sbjct: 87  SETPLIKVADPKVLGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSAALQKL 139


>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 154

 Score = 31.9 bits (69), Expect = 0.057
 Identities = 17/74 (22%), Positives = 35/74 (47%)
 Frame = +1

Query: 85  VNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQ 264
           +N  + + +K A     ++ G+ E  KA+ K +  L +LA +         +  LC ++ 
Sbjct: 37  LNKKMMKTVKKASKQKHILRGVKEVVKAVRKGEKGLVILAGDISPMDVISHIPVLCEDNN 96

Query: 265 IPLVKVDNNKKLGE 306
           +P +   + + LGE
Sbjct: 97  VPYLYTVSKELLGE 110


>SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 259

 Score = 31.1 bits (67), Expect = 0.099
 Identities = 14/54 (25%), Positives = 27/54 (50%)
 Frame = +1

Query: 142 HGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLG 303
           +GL+     ++ ++A L ++A + D       + ALC +  +P   V N  +LG
Sbjct: 134 YGLNHVVALIEAKKAKLVLIASDVDPIELVVFLPALCKKMGVPYAIVKNKARLG 187


>SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 404

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = -1

Query: 405 RWSFLAEIFDDNTGAADNFPCLAIL 331
           RW +LA +F  N  A  N P + +L
Sbjct: 257 RWEYLASLFTSNFTAVHNIPSVPLL 281


>SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase
           Ubp21|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1129

 Score = 25.4 bits (53), Expect = 4.9
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = +1

Query: 40  VEVPTNPILSGNNMDVNVALQEVLKTALIHGGLVHGLH 153
           +E+   P LS    D + +   VL   L+HGG +HG H
Sbjct: 445 LEIDLEPYLS-ETADKSESHVYVLHGVLVHGGDLHGGH 481


>SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 923

 Score = 25.0 bits (52), Expect = 6.5
 Identities = 10/35 (28%), Positives = 19/35 (54%)
 Frame = +2

Query: 200 WLRTVMKPHTRNLFRLCATNIRFHWSRLTTTKSLE 304
           ++ +V  P    L+RLC++  R  W     ++SL+
Sbjct: 220 FILSVFHPDAARLYRLCSSLKRICWELYDPSRSLD 254


>SPAPJ696.01c |vps17||retromer complex subunit
           Vps17|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 549

 Score = 25.0 bits (52), Expect = 6.5
 Identities = 10/28 (35%), Positives = 20/28 (71%), Gaps = 1/28 (3%)
 Frame = -3

Query: 373 QHRSSRQFSLPCHPCQSCRD-QPILQAF 293
           + ++ +QF LP  PCQ+  + +PI+++F
Sbjct: 226 KRKALKQFPLPPDPCQALANLRPIVKSF 253


>SPCP31B10.07 |eft202||translation elongation factor 2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 15/55 (27%), Positives = 24/55 (43%)
 Frame = -3

Query: 229 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 65
           C+    S   +HIV  A    L    +D  +DH G+ L ++P  V +      P+
Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571


>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1133

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 9/20 (45%), Positives = 14/20 (70%)
 Frame = -3

Query: 136 DHHGLKLSLAPLAVLHSRPC 77
           D  GL  ++  L+++HSRPC
Sbjct: 529 DEMGLGKTIEVLSLIHSRPC 548


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
           elongation factor 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 15/55 (27%), Positives = 24/55 (43%)
 Frame = -3

Query: 229 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 65
           C+    S   +HIV  A    L    +D  +DH G+ L ++P  V +      P+
Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571


>SPBC2G2.08 |ade9||C-1-
           tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr
           ogenase/methylenetetrahydrofolatecyclohydrolase/formylte
           trahydrofolatesynthetase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 969

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 11/20 (55%), Positives = 15/20 (75%)
 Frame = +2

Query: 347 GKLSAAPVLSSKISARKLQR 406
           G  S+A +L+ K+ ARKLQR
Sbjct: 28  GTSSSANILNGKLLARKLQR 47


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,987,601
Number of Sequences: 5004
Number of extensions: 38349
Number of successful extensions: 145
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 202220600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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