Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0365
(722 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 88 6e-18
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 84 8e-17
At4g17060.1 68417.m02572 expressed protein 29 4.1
At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative ... 28 7.2
At5g54650.2 68418.m06805 formin homology 2 domain-containing pro... 27 9.5
At5g54650.1 68418.m06804 formin homology 2 domain-containing pro... 27 9.5
At5g11250.1 68418.m01314 disease resistance protein (TIR-NBS-LRR... 27 9.5
At5g10530.1 68418.m01219 lectin protein kinase, putative similar... 27 9.5
At4g26300.1 68417.m03783 arginyl-tRNA synthetase, putative / arg... 27 9.5
At4g26090.1 68417.m03756 disease resistance protein RPS2 (CC-NBS... 27 9.5
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g... 27 9.5
At4g20730.1 68417.m03013 filament protein-related similar to Cyt... 27 9.5
At4g02560.1 68417.m00350 homeobox protein LUMINIDEPENDENS (LD) i... 27 9.5
At3g10100.1 68416.m01210 filament protein-related similar to YEA... 27 9.5
At2g17010.1 68415.m01961 mechanosensitive ion channel domain-con... 27 9.5
At1g19130.1 68414.m02379 expressed protein 27 9.5
>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 87.8 bits (208), Expect = 6e-18
Identities = 57/157 (36%), Positives = 81/157 (51%)
Frame = +2
Query: 125 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 304
MS K EKP L G RIKTRKR+ DP F DA+VQ GDL+ K ++S S L
Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIES--SDL 57
Query: 305 DYRRYGEVIFDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMR 484
++ RYG++ F+V+ GG PG S +GE + + + + K++R
Sbjct: 58 NFTRYGDIFFEVIFIGGRTQPGTVKSDEGE--RHTYSVIDCEPKREAILPSVVYIQKILR 115
Query: 485 RYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARMTAL 595
R +L K E ++ L L+ F+ +R KLA TAL
Sbjct: 116 RKPFLIKNLENVTRRFLQSLELFEENERKKLAIFTAL 152
>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|P47823
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Oryctolagus
cuniculus}; contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 84.2 bits (199), Expect = 8e-17
Identities = 58/157 (36%), Positives = 80/157 (50%)
Frame = +2
Query: 125 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 304
MS K EKP L G RIKTRKR+ DP F DALVQ GDL+ + L+S S L
Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDALVQIYLDNAGDLELVARSLES--SDL 57
Query: 305 DYRRYGEVIFDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMR 484
++ RYG++ F+V+ GG G S +GE + I + + K++R
Sbjct: 58 NFSRYGDIFFEVVFIGGRTQTGSVKSDEGE--RHPYSIIDCEPKREAILPSVVYIQKILR 115
Query: 485 RYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARMTAL 595
R +L K E ++ L L+ F+ +R KLA TAL
Sbjct: 116 RKAFLIKNLENVTRRFLQSLELFEENERKKLAIFTAL 152
>At4g17060.1 68417.m02572 expressed protein
Length = 310
Score = 28.7 bits (61), Expect = 4.1
Identities = 17/64 (26%), Positives = 30/64 (46%)
Frame = +2
Query: 5 NTKNSCSLLSHSQVNRLSSGETAPLD*VVKGIRLIRTIYCMSQKVEKPVLSGQRIKTRKR 184
N S S H Q+N + E+ P+D VV ++++ ++ +VE + R + R
Sbjct: 215 NGYGSSSRFKHEQMNAAAEVESDPIDEVVSSVKMLTEMFV---RVENSKMEMMREMEKSR 271
Query: 185 DEKE 196
E E
Sbjct: 272 MEME 275
>At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative
similar to GTL1 [Arabidopsis thaliana] GI:2664198
Length = 669
Score = 27.9 bits (59), Expect = 7.2
Identities = 15/54 (27%), Positives = 25/54 (46%)
Frame = -3
Query: 480 ISFTNTCSKFRMVSISSLAEKMQVLVLGDSPSIDTEPPGSSRPPAMSTSNMTSP 319
+S S R +I SL +K+ + PS+ ++PP +PP T + P
Sbjct: 321 MSQERAASASRDAAIISLIQKITGHTIQLPPSLSSQPPPPYQPPPAVTKRVAEP 374
>At5g54650.2 68418.m06805 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 27.5 bits (58), Expect = 9.5
Identities = 17/40 (42%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
Frame = -3
Query: 399 GDSPSID-TEPPGSSRPPAMSTSNMTSP*RR*SSFDPAES 283
G SPS + PP SR P + SP R SSF P+ S
Sbjct: 143 GSSPSPSPSRPPKRSRGPPRPPTRPKSPPPRKSSFPPSRS 182
>At5g54650.1 68418.m06804 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 27.5 bits (58), Expect = 9.5
Identities = 17/40 (42%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
Frame = -3
Query: 399 GDSPSID-TEPPGSSRPPAMSTSNMTSP*RR*SSFDPAES 283
G SPS + PP SR P + SP R SSF P+ S
Sbjct: 143 GSSPSPSPSRPPKRSRGPPRPPTRPKSPPPRKSSFPPSRS 182
>At5g11250.1 68418.m01314 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1189
Score = 27.5 bits (58), Expect = 9.5
Identities = 21/84 (25%), Positives = 38/84 (45%), Gaps = 6/84 (7%)
Frame = +2
Query: 392 ESPKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLE----KMFEEEMK--KVLVYLKGF 553
E+ K N + ++D +Q+F+ + + K +E + ++ +K KVLV L G
Sbjct: 292 ENIKANYTRPTGSDDYSAKLQLQQMFMSQITKQKDIEIPHLGVAQDRLKDKKVLVVLDGV 351
Query: 554 DPEQRIKLARMTALWIGNGMRASI 625
+ ++ A W G G R I
Sbjct: 352 NQSVQLDAMAKEAWWFGPGSRIII 375
>At5g10530.1 68418.m01219 lectin protein kinase, putative similar to
receptor lectin kinase 3 [Arabidopsis thaliana]
gi|4100060|gb|AAD00733; contains protein kinase domain,
Pfam:PF00069; contains legume lectins alpha and beta
domains, Pfam:PF00138 and Pfam:PF00139
Length = 651
Score = 27.5 bits (58), Expect = 9.5
Identities = 18/62 (29%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = +2
Query: 176 RKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL-DYRRYGEVIFDVLIAG 352
+++ +K+K + ++ + LER G YK L SA + D R+ GE F + G
Sbjct: 293 KRKQQKKKAEETENLTSINEDLERGAGPRKFTYKDLASAANNFADDRKLGEGGFGAVYRG 352
Query: 353 GL 358
L
Sbjct: 353 YL 354
>At4g26300.1 68417.m03783 arginyl-tRNA synthetase, putative /
arginine--tRNA ligase, putative similar to SP|P37880
Arginyl-tRNA synthetase (EC 6.1.1.19) (Arginine--tRNA
ligase) (ArgRS) {Cricetulus longicaudatus}; contains
Pfam profiles PF00750: arginyl-tRNA synthetase, PF03485:
arginyl-tRNA synthetase N-terminal domain
Length = 642
Score = 27.5 bits (58), Expect = 9.5
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +2
Query: 461 QVFVKLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARM 586
QVF K + L++ F+E+ ++ +V L+G DP R A++
Sbjct: 260 QVFYKASKHKFDLDEAFKEKAQQAVVRLQGGDPVYRKAWAKI 301
>At4g26090.1 68417.m03756 disease resistance protein RPS2
(CC-NBS-LRR class), putative domain signature CC-NBS-LRR
exists, suggestive of a disease resistance protein.
identical to RPS2 (gi:13661831)
Length = 909
Score = 27.5 bits (58), Expect = 9.5
Identities = 15/59 (25%), Positives = 25/59 (42%)
Frame = +2
Query: 446 MRNFEQVFVKLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARMTALWIGNGMRAS 622
M+ VF L Y LE + ++ +Y F E I++ ++ W+G G S
Sbjct: 382 MKGMNYVFALLKFSYDNLES---DLLRSCFLYCALFPEEHSIEIEQLVEYWVGEGFLTS 437
>At4g25000.1 68417.m03587 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative similar to
alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
GI:21670851; contains Pfam profile PF00128: Alpha
amylase, catalytic domain
Length = 423
Score = 27.5 bits (58), Expect = 9.5
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +2
Query: 185 DEKEKYDPNGFRDALVQGLERAGGDLDAAYKY 280
D K YD N R L Q +E AGG + A+ +
Sbjct: 238 DGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDF 269
>At4g20730.1 68417.m03013 filament protein-related similar to
Cytadherence high molecular weight protein 2 (SP:P47460)
[Mycoplasma genitalium]; similar to YEAST NUF1
protein (Spindle poly body spacer protein SPC110)
(SP:P32380) {Saccharomyces cerevisiae}; also
SP|Q9UKX2|MYH2_HUMAN Myosin heavy chain, skeletal
muscle, SP|P31732|OV71_ONCVO Muscle cell intermediate
filament protein SP|P12882|MYH1_HUMAN Myosin heavy
chain, skeletal muscle,. SP|Q17107|AV71_ACAVI Muscle
cell intermediate filament protein
Length = 800
Score = 27.5 bits (58), Expect = 9.5
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +1
Query: 472 ETDASVQVLGKNVRRGNEKGSGLLERLRSRTAHQAG 579
+ D +V LG + R EKG G+ + + R+A +AG
Sbjct: 455 DKDETVAELGISRRSREEKGKGVATQSKKRSASEAG 490
>At4g02560.1 68417.m00350 homeobox protein LUMINIDEPENDENS (LD)
identical to Homeobox protein LUMINIDEPENDENS
(Swiss-Prot:Q38796) [Arabidopsis thaliana]
Length = 953
Score = 27.5 bits (58), Expect = 9.5
Identities = 21/61 (34%), Positives = 30/61 (49%), Gaps = 2/61 (3%)
Frame = +3
Query: 399 PRPTPASSPPMRIWTPCEISNRYS*N*CVGTSTWKKCSK-RK*KRFW-ST*KASIPNSAS 572
P PTP+++P M W I N +S VGT+ + ++ R W T + SIP A
Sbjct: 753 PSPTPSTNPGMSGWGQEGIRNPFSRQNQVGTAVARSGTQLRVGSMQWHQTNEQSIPRHAP 812
Query: 573 S 575
S
Sbjct: 813 S 813
>At3g10100.1 68416.m01210 filament protein-related similar to YEAST
NUF1 protein (Spindle poly body spacer protein SPC110)
(SP:P32380) {Saccharomyces cerevisiae}; similar to
Myosin heavy chain, smooth muscle isoform (SMMHC)
(SP:P35749) {Homo sapiens}
Length = 1004
Score = 27.5 bits (58), Expect = 9.5
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +1
Query: 487 VQVLGKNVRRGNEKGSGLLERLRSRTAHQAGAHDCTVDR 603
V LG + R EKG GL + + R+A +AG + +
Sbjct: 459 VAELGASRRSREEKGKGLASQSKKRSASEAGLDEAAAPK 497
>At2g17010.1 68415.m01961 mechanosensitive ion channel
domain-containing protein / MS ion channel
domain-containing protein contains Pfam profile PF00924:
Mechanosensitive ion channel
Length = 779
Score = 27.5 bits (58), Expect = 9.5
Identities = 16/60 (26%), Positives = 26/60 (43%)
Frame = +2
Query: 287 SAGSKLDYRRYGEVIFDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQV 466
+A + D R V F+V AGG + G S S +++ N+D ++ E V
Sbjct: 165 TARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQPQLQEEEVV 224
>At1g19130.1 68414.m02379 expressed protein
Length = 187
Score = 27.5 bits (58), Expect = 9.5
Identities = 15/51 (29%), Positives = 24/51 (47%)
Frame = +2
Query: 359 LLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMF 511
LLP GSVS P T F E + +R+ ++ ++L++MF
Sbjct: 59 LLPSGSVSRLHRIPMAETWHFYLGEPLTVIRSLSTRSLQTSGLVRFLQRMF 109
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,537,816
Number of Sequences: 28952
Number of extensions: 375818
Number of successful extensions: 1321
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1228
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1316
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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