BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0357 (721 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccha... 29 0.67 SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizos... 27 2.7 SPAC1B3.01c |||uracil phosphoribosyltransferase |Schizosaccharom... 26 6.2 SPAC343.13 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 25 8.2 SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe... 25 8.2 >SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 29.1 bits (62), Expect = 0.67 Identities = 14/42 (33%), Positives = 21/42 (50%) Frame = +2 Query: 158 SSPYISKFISNSYNNNTVYIYVFKSGEGSLIADELYKRARDR 283 +SPYI K+ YNN + + G+ D +YKR R + Sbjct: 133 TSPYIVKYYGACYNNAECQLNIAMEYCGAGSLDAIYKRVRSQ 174 >SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 286 Score = 27.1 bits (57), Expect = 2.7 Identities = 19/43 (44%), Positives = 24/43 (55%), Gaps = 2/43 (4%) Frame = +3 Query: 525 SIEQRTRSAQAAAQGTQRNSSSLRRVPTAPLRADDTL--TSNS 647 S+EQ +S + A Q R SSLR + T + DDT TSNS Sbjct: 188 SLEQIKKSQEEALQNLSREISSLRCLQT-DSKKDDTFATTSNS 229 Score = 25.4 bits (53), Expect = 8.2 Identities = 10/42 (23%), Positives = 22/42 (52%), Gaps = 1/42 (2%) Frame = -3 Query: 329 WSCATLPPNY-SPQFSRDHERAYKVRRRSMNLRLI*IHKYIQ 207 WS +L Y +P F + AY+ + +++ + + HK ++ Sbjct: 84 WSAYSLVKKYIAPMFRAPSQNAYEADKNALDAKFLEAHKILE 125 >SPAC1B3.01c |||uracil phosphoribosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 219 Score = 25.8 bits (54), Expect = 6.2 Identities = 10/32 (31%), Positives = 17/32 (53%) Frame = +1 Query: 295 GL*FGGKVAQLQKVRGARKVNRGTARACRSVR 390 G+ F G++ + +R + +G CRSVR Sbjct: 72 GVMFDGRICGVSIMRAGESMEQGLRECCRSVR 103 >SPAC343.13 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces pombe|chr 1|||Manual Length = 526 Score = 25.4 bits (53), Expect = 8.2 Identities = 12/33 (36%), Positives = 17/33 (51%) Frame = +3 Query: 582 SSSLRRVPTAPLRADDTLTSNSHHCSHKVGREM 680 +S L+ +P P LT+ SH SHK R + Sbjct: 321 NSVLKSLPALPDELFQKLTTGSHAISHKEARTL 353 >SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe|chr 1|||Manual Length = 371 Score = 25.4 bits (53), Expect = 8.2 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = +2 Query: 158 SSPYISKFISNSYNNNTVYIYVFKSGEGSLIAD 256 SSP SKF S+ +++ + G GSLI D Sbjct: 87 SSPPSSKFGGPSFGTPKPFLHTNRLGTGSLIED 119 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,524,993 Number of Sequences: 5004 Number of extensions: 43574 Number of successful extensions: 120 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 115 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 120 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 337208592 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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