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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0357
         (721 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccha...    29   0.67 
SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizos...    27   2.7  
SPAC1B3.01c |||uracil phosphoribosyltransferase |Schizosaccharom...    26   6.2  
SPAC343.13 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces...    25   8.2  
SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe...    25   8.2  

>SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score = 29.1 bits (62), Expect = 0.67
 Identities = 14/42 (33%), Positives = 21/42 (50%)
 Frame = +2

Query: 158 SSPYISKFISNSYNNNTVYIYVFKSGEGSLIADELYKRARDR 283
           +SPYI K+    YNN    + +     G+   D +YKR R +
Sbjct: 133 TSPYIVKYYGACYNNAECQLNIAMEYCGAGSLDAIYKRVRSQ 174


>SPBC13G1.03c |pex14||peroxisomal membrane anchor
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 286

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 19/43 (44%), Positives = 24/43 (55%), Gaps = 2/43 (4%)
 Frame = +3

Query: 525 SIEQRTRSAQAAAQGTQRNSSSLRRVPTAPLRADDTL--TSNS 647
           S+EQ  +S + A Q   R  SSLR + T   + DDT   TSNS
Sbjct: 188 SLEQIKKSQEEALQNLSREISSLRCLQT-DSKKDDTFATTSNS 229



 Score = 25.4 bits (53), Expect = 8.2
 Identities = 10/42 (23%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
 Frame = -3

Query: 329 WSCATLPPNY-SPQFSRDHERAYKVRRRSMNLRLI*IHKYIQ 207
           WS  +L   Y +P F    + AY+  + +++ + +  HK ++
Sbjct: 84  WSAYSLVKKYIAPMFRAPSQNAYEADKNALDAKFLEAHKILE 125


>SPAC1B3.01c |||uracil phosphoribosyltransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 219

 Score = 25.8 bits (54), Expect = 6.2
 Identities = 10/32 (31%), Positives = 17/32 (53%)
 Frame = +1

Query: 295 GL*FGGKVAQLQKVRGARKVNRGTARACRSVR 390
           G+ F G++  +  +R    + +G    CRSVR
Sbjct: 72  GVMFDGRICGVSIMRAGESMEQGLRECCRSVR 103


>SPAC343.13 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 526

 Score = 25.4 bits (53), Expect = 8.2
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = +3

Query: 582 SSSLRRVPTAPLRADDTLTSNSHHCSHKVGREM 680
           +S L+ +P  P      LT+ SH  SHK  R +
Sbjct: 321 NSVLKSLPALPDELFQKLTTGSHAISHKEARTL 353


>SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 371

 Score = 25.4 bits (53), Expect = 8.2
 Identities = 13/33 (39%), Positives = 18/33 (54%)
 Frame = +2

Query: 158 SSPYISKFISNSYNNNTVYIYVFKSGEGSLIAD 256
           SSP  SKF   S+     +++  + G GSLI D
Sbjct: 87  SSPPSSKFGGPSFGTPKPFLHTNRLGTGSLIED 119


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,524,993
Number of Sequences: 5004
Number of extensions: 43574
Number of successful extensions: 120
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 120
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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