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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0357
         (721 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g48980.1 68414.m05491 hypothetical protein                          31   1.0  
At4g28520.2 68417.m04079 12S seed storage protein, putative / cr...    27   9.5  
At4g28520.1 68417.m04080 12S seed storage protein, putative / cr...    27   9.5  

>At1g48980.1 68414.m05491 hypothetical protein
          Length = 264

 Score = 30.7 bits (66), Expect = 1.0
 Identities = 19/71 (26%), Positives = 31/71 (43%)
 Frame = +3

Query: 468 SAAREVAIEPAHYTREHAASIEQRTRSAQAAAQGTQRNSSSLRRVPTAPLRADDTLTSNS 647
           S  +EVA++ +H T E   S E+  + +   A G+   +  L R     +RA +      
Sbjct: 27  SQEQEVAVDFSHMTLEDELSEEEDHKDSSREAFGSSLENHKLSRKQRTHIRAINVKRKRD 86

Query: 648 HHCSHKVGREM 680
             C  KV  E+
Sbjct: 87  FVCLEKVNGEL 97


>At4g28520.2 68417.m04079 12S seed storage protein, putative /
           cruciferin, putative strong similarity to SP|P33525
           Cruciferin CRU1 precursor (11S globulin) (12S storage
           protein) from Brassica napus; contains Pfam profile
           PF00190 Cupin and Prosite 11-S plant seed storage
           proteins signature PS00305 isoform contains
           non-consensus AC acceptor splice site at intron 3
          Length = 394

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 14/43 (32%), Positives = 20/43 (46%)
 Frame = +2

Query: 527 YRAEDAERASSCPGDAAEQQLASPRSHRTAPSR*HADVEQPPL 655
           Y +E+     S  G+  E+ + S RSH        ADV +P L
Sbjct: 319 YESEEWRHPRSPQGNGLEETICSMRSHENIDDPARADVYKPSL 361


>At4g28520.1 68417.m04080 12S seed storage protein, putative /
           cruciferin, putative strong similarity to SP|P33525
           Cruciferin CRU1 precursor (11S globulin) (12S storage
           protein) from Brassica napus; contains Pfam profile
           PF00190 Cupin and Prosite 11-S plant seed storage
           proteins signature PS00305 isoform contains
           non-consensus AC acceptor splice site at intron 3
          Length = 524

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 14/43 (32%), Positives = 20/43 (46%)
 Frame = +2

Query: 527 YRAEDAERASSCPGDAAEQQLASPRSHRTAPSR*HADVEQPPL 655
           Y +E+     S  G+  E+ + S RSH        ADV +P L
Sbjct: 319 YESEEWRHPRSPQGNGLEETICSMRSHENIDDPARADVYKPSL 361


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,779,758
Number of Sequences: 28952
Number of extensions: 251309
Number of successful extensions: 654
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 642
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 654
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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