BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0351 (695 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 311 7e-86 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 308 4e-85 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 266 3e-72 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 217 2e-57 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 160 2e-40 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 130 2e-31 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 113 2e-26 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 104 1e-23 SPBC215.10 |||haloacid dehalogenase-like hydrolase|Schizosacchar... 29 0.48 SPBC2D10.17 |clr1||cryptic loci regulator Clr1|Schizosaccharomyc... 29 0.84 SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ... 26 4.5 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 5.9 SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 26 5.9 SPAC1142.08 |fhl1|SPAC8C9.01|fork head transcription factor Fhl1... 25 7.8 SPCC320.09 |hem15||ferrochelatase|Schizosaccharomyces pombe|chr ... 25 7.8 SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 25 7.8 SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces p... 25 7.8 SPAC4G9.04c |||cleavage and polyadenylation specificity factor |... 25 7.8 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 311 bits (763), Expect = 7e-86 Identities = 150/193 (77%), Positives = 168/193 (87%) Frame = +2 Query: 2 VLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLA 181 VLIFDLGGGTFDVS+LTIE+GIFEVK+TAGDTHLGGEDFD+R+VNHF+QEFKRK KKD+ Sbjct: 193 VLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDSRLVNHFIQEFKRKNKKDIT 252 Query: 182 TNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTM 361 N RA+RRLRTACERAKRTLSSS QASIEIDSLFEGIDFYTSITRARFEEL ADLFR TM Sbjct: 253 GNARAVRRLRTACERAKRTLSSSAQASIEIDSLFEGIDFYTSITRARFEELCADLFRKTM 312 Query: 362 EPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEXXXXXXX 541 EPVE+ LRD+K+DKA +++IVLVGGSTRIP+VQKL+ DFFNGKE KSINPDE Sbjct: 313 EPVERVLRDSKVDKASVNEIVLVGGSTRIPRVQKLVSDFFNGKEPCKSINPDEAVAYGAA 372 Query: 542 XXXXILHGDKSER 580 +L GD SE+ Sbjct: 373 VQAAVLTGDTSEK 385 Score = 39.5 bits (88), Expect = 4e-04 Identities = 18/20 (90%), Positives = 18/20 (90%) Frame = +1 Query: 622 GIETAGSVMTTLIKRNTTIP 681 GIETAG VMT LIKRNTTIP Sbjct: 400 GIETAGGVMTPLIKRNTTIP 419 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 308 bits (757), Expect = 4e-85 Identities = 149/193 (77%), Positives = 168/193 (87%) Frame = +2 Query: 2 VLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLA 181 VLIFDLGGGTFDVS+LTIE+GIFEVK+TAGDTHLGGEDFD+R+VNHF QEFKRK KKD+ Sbjct: 193 VLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDSRLVNHFAQEFKRKNKKDIT 252 Query: 182 TNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTM 361 N RA+RRLRTACERAKRTLSSS QASIEIDSL+EGIDFYTSITRARFEEL ADLFR+TM Sbjct: 253 GNARAVRRLRTACERAKRTLSSSAQASIEIDSLYEGIDFYTSITRARFEELCADLFRNTM 312 Query: 362 EPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEXXXXXXX 541 EPVEK LRD+K+DK+ +++IVLVGGSTRIP++QKL+ DFFNGKE KSINPDE Sbjct: 313 EPVEKVLRDSKIDKSSVNEIVLVGGSTRIPRIQKLVSDFFNGKEPCKSINPDEAVAYGAA 372 Query: 542 XXXXILHGDKSER 580 IL GD SE+ Sbjct: 373 VQAAILVGDTSEK 385 Score = 39.5 bits (88), Expect = 4e-04 Identities = 18/20 (90%), Positives = 18/20 (90%) Frame = +1 Query: 622 GIETAGSVMTTLIKRNTTIP 681 GIETAG VMT LIKRNTTIP Sbjct: 400 GIETAGGVMTPLIKRNTTIP 419 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 266 bits (651), Expect = 3e-72 Identities = 122/173 (70%), Positives = 150/173 (86%) Frame = +2 Query: 2 VLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLA 181 VLIFDLGGGTFDVS+L I+ G+FEV +TAGDTHLGGEDFDN +V HF+QEFKRK K D++ Sbjct: 197 VLIFDLGGGTFDVSLLKIQGGVFEVLATAGDTHLGGEDFDNALVEHFIQEFKRKQKIDIS 256 Query: 182 TNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTM 361 + RALRRLR+ACERAKR LSS TQ ++E+DSL GIDF +SITRARFE++NA F++T+ Sbjct: 257 DDPRALRRLRSACERAKRALSSVTQTTVEVDSLSNGIDFSSSITRARFEDINATTFKATI 316 Query: 362 EPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDE 520 +PV K L+D+K+ KA +HDIVLVGGSTRIPKVQ+L+ DFF+G+ LNKSINPDE Sbjct: 317 DPVAKVLKDSKVPKADVHDIVLVGGSTRIPKVQRLVSDFFDGRALNKSINPDE 369 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 217 bits (529), Expect = 2e-57 Identities = 98/190 (51%), Positives = 143/190 (75%) Frame = +2 Query: 2 VLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLA 181 ++++DLGGGTFDVS+L+I++G+FEV +T+GDTHLGGEDFDNR++N+ + + RK D+ Sbjct: 225 IVVYDLGGGTFDVSLLSIDNGVFEVLATSGDTHLGGEDFDNRVINYLARTYNRKNNVDVT 284 Query: 182 TNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTM 361 + +A+ +L+ E+AKRTLSS IEI+S F G DF +++RA+FEE+N DLF+ T+ Sbjct: 285 KDLKAMGKLKREVEKAKRTLSSQKSVRIEIESFFNGQDFSETLSRAKFEEINMDLFKKTL 344 Query: 362 EPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEXXXXXXX 541 +PVE+ L+D+ + K++I DIVLVGGSTRIPKVQ+LL+ FF GK+ +K INPDE Sbjct: 345 KPVEQVLKDSNLKKSEIDDIVLVGGSTRIPKVQELLESFF-GKKASKGINPDEAVAYGAA 403 Query: 542 XXXXILHGDK 571 +L G++ Sbjct: 404 VQAGVLSGEE 413 Score = 32.7 bits (71), Expect = 0.052 Identities = 15/20 (75%), Positives = 15/20 (75%) Frame = +1 Query: 622 GIETAGSVMTTLIKRNTTIP 681 GIET G VMT LI RNT IP Sbjct: 429 GIETTGGVMTKLIGRNTPIP 448 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 160 bits (388), Expect = 2e-40 Identities = 80/192 (41%), Positives = 124/192 (64%), Gaps = 4/192 (2%) Frame = +2 Query: 2 VLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLA 181 V +FDLGGGTFD+SIL + +G+FEV+ST GDTHLGGEDFD +V H V+ FK+ DL+ Sbjct: 237 VAVFDLGGGTFDISILELNNGVFEVRSTNGDTHLGGEDFDVALVRHIVETFKKNEGLDLS 296 Query: 182 TNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGI----DFYTSITRARFEELNADLF 349 ++ A++R+R A E+AK LSS ++ I + + I+RA+FE+L L Sbjct: 297 KDRLAVQRIREAAEKAKCELSSLSKTDISLPFITADATGPKHINMEISRAQFEKLVDPLV 356 Query: 350 RSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEXXX 529 R T++P +++L+DA + ++I++++LVGG TR+P+V + ++ F +E KS+NPDE Sbjct: 357 RRTIDPCKRALKDANLQTSEINEVILVGGMTRMPRVVETVKSIFK-REPAKSVNPDEAVA 415 Query: 530 XXXXXXXXILHG 565 +L G Sbjct: 416 IGAAIQGGVLSG 427 Score = 33.5 bits (73), Expect = 0.030 Identities = 15/20 (75%), Positives = 15/20 (75%) Frame = +1 Query: 622 GIETAGSVMTTLIKRNTTIP 681 GIET G V T LI RNTTIP Sbjct: 443 GIETLGGVFTRLINRNTTIP 462 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 130 bits (314), Expect = 2e-31 Identities = 66/173 (38%), Positives = 103/173 (59%) Frame = +2 Query: 2 VLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLA 181 V I D G + VSI+ G F +KST D +LG + D ++++F EFK KYK D+ Sbjct: 201 VAIVDFGHSNYSVSIVEFSRGQFHIKSTVCDRNLGSRNMDKALIDYFAAEFKEKYKIDVL 260 Query: 182 TNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTM 361 +N +A RL TA ER K+ LS++ A + ++ + ID + I R+ FEEL L Sbjct: 261 SNPKATFRLATAVERLKKVLSANANAPLNVEMIMNDIDASSFIKRSDFEELIKPLLERLT 320 Query: 362 EPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDE 520 P+EK+L A + K ++ I +VGG TR+P V++++ ++F GK L+ ++N DE Sbjct: 321 PPIEKALELAGIKKEDLYSIEMVGGCTRVPIVKEVIANYF-GKGLSFTLNQDE 372 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 113 bits (273), Expect = 2e-26 Identities = 54/168 (32%), Positives = 99/168 (58%) Frame = +2 Query: 2 VLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLA 181 V++ + G +VS+++++ G+ + ++ D +LGGE + +VN F +EF++K D Sbjct: 219 VVVANFGATRSEVSVVSVKGGLMTILASVHDENLGGEQLTDVLVNFFAKEFEKKNGIDPR 278 Query: 182 TNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTM 361 N R+L +LR CE KR LS+ T AS +DSL +GIDF++SI R R++ + Sbjct: 279 KNARSLAKLRAQCEITKRVLSNGTTASAAVDSLADGIDFHSSINRLRYDLAASATLNRMA 338 Query: 362 EPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKS 505 + V +++ A M+ I +++L GG++ PK+ L++ F + + +S Sbjct: 339 DLVTEAVEKANMEPFDISEVILAGGASNTPKLTSLMESIFPEQTIIRS 386 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 104 bits (249), Expect = 1e-23 Identities = 56/166 (33%), Positives = 97/166 (58%), Gaps = 2/166 (1%) Frame = +2 Query: 29 TFDVSILTIEDGIFEVKSTA-GDT-HLGGEDFDNRMVNHFVQEFKRKYKKDLATNKRALR 202 T + S L + I +++ A G T L G + + +++ F +K+ DL+ N RAL Sbjct: 236 TVEGSSLGKKQNITRIRALASGFTLKLSGNEINRKLIGFMKNSFYQKHGIDLSHNHRALA 295 Query: 203 RLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSL 382 RL R K LS++++A I+ L +GIDF ITR+ E L D+ + +EP+ K+L Sbjct: 296 RLEKEALRVKHILSANSEAIASIEELADGIDFRLKITRSVLESLCKDMEDAAVEPINKAL 355 Query: 383 RDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDE 520 + A + ++I+ I+L GG++RIP +Q L D+ + +++K++N DE Sbjct: 356 KKANLTFSEINSIILFGGASRIPFIQSTLADYVSSDKISKNVNADE 401 >SPBC215.10 |||haloacid dehalogenase-like hydrolase|Schizosaccharomyces pombe|chr 2|||Manual Length = 302 Score = 29.5 bits (63), Expect = 0.48 Identities = 17/43 (39%), Positives = 23/43 (53%), Gaps = 1/43 (2%) Frame = +3 Query: 162 NTKRTSLPTRELLGVCVLHVRGQR-GPCHRPHKRALR*ILSLR 287 N K+ LP E + + + V G GP H+PH R LR + LR Sbjct: 9 NQKKNVLPKPEDIQLIICDVDGTLLGPDHKPHPRNLRALKYLR 51 >SPBC2D10.17 |clr1||cryptic loci regulator Clr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1238 Score = 28.7 bits (61), Expect = 0.84 Identities = 16/37 (43%), Positives = 21/37 (56%) Frame = -3 Query: 120 SKSSPPKWVSPAVDFTSKIPSSMVRMDTSKVPPPRSK 10 +KSSP K VSP V SK +S V + S +P +K Sbjct: 203 NKSSPVKHVSPNVKNNSKKTASSVNSNHSSIPSSITK 239 >SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 932 Score = 26.2 bits (55), Expect = 4.5 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = -1 Query: 416 RGFEPYPSWHHGETSPLAP 360 + +P P+ HG+TSPL+P Sbjct: 420 KAIKPMPTSIHGQTSPLSP 438 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = -1 Query: 605 TSSNSRSCTSQTCHRAR*QPGQLHHKLRP 519 T S +R C S +C R Q +LH K++P Sbjct: 381 TCSRNRLCYSISCPRRLEQQARLHLKVQP 409 >SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 538 Score = 25.8 bits (54), Expect = 5.9 Identities = 17/53 (32%), Positives = 27/53 (50%) Frame = +2 Query: 275 SLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVG 433 SL G+D+YT I E +A +S+ E +K D + D++ D + VG Sbjct: 331 SLARGLDYYTGIIYEAVTEASAPKIKSSAE--KKKSADPEADRSN-DDSIGVG 380 >SPAC1142.08 |fhl1|SPAC8C9.01|fork head transcription factor Fhl1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 743 Score = 25.4 bits (53), Expect = 7.8 Identities = 11/26 (42%), Positives = 14/26 (53%) Frame = +1 Query: 121 QSHGEPLCPGVQEEIQKGPRYQQESS 198 QS G+PL PG + GP + SS Sbjct: 599 QSIGDPLPPGAMANVSAGPSSVRSSS 624 >SPCC320.09 |hem15||ferrochelatase|Schizosaccharomyces pombe|chr 3|||Manual Length = 384 Score = 25.4 bits (53), Expect = 7.8 Identities = 12/27 (44%), Positives = 14/27 (51%) Frame = -3 Query: 156 LNSWTKWFTMRLSKSSPPKWVSPAVDF 76 LN K+ SK P W+SPA DF Sbjct: 256 LNYKNKFVNAWQSKVGPLPWMSPATDF 282 >SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1628 Score = 25.4 bits (53), Expect = 7.8 Identities = 15/46 (32%), Positives = 23/46 (50%) Frame = +2 Query: 128 MVNHFVQEFKRKYKKDLATNKRALRRLRTACERAKRTLSSSTQASI 265 +++ F EFK++ K D + L+ L + C R R SS SI Sbjct: 711 ILSSFSNEFKKRLKSDGTSIAHWLQGLASFCGRVFRRYSSLDCTSI 756 >SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 591 Score = 25.4 bits (53), Expect = 7.8 Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 4/34 (11%) Frame = +3 Query: 342 ICSGLPWSQ----WRSLSVMPRWIRLKSTILYWW 431 ICS LP + W + S PR+ R S ++ WW Sbjct: 133 ICSALPAAGSIYLWAAESAGPRFGRFVSFLVAWW 166 >SPAC4G9.04c |||cleavage and polyadenylation specificity factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 638 Score = 25.4 bits (53), Expect = 7.8 Identities = 10/27 (37%), Positives = 18/27 (66%) Frame = +3 Query: 24 AVPSTCPSLPSRMVSSR*NPPPATPTW 104 ++PST P++PS +S + PP T ++ Sbjct: 299 SIPSTIPTIPSAYSASVSSQPPLTHSY 325 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,997,568 Number of Sequences: 5004 Number of extensions: 63122 Number of successful extensions: 244 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 223 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 242 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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