BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0342 (724 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10 |Schizo... 139 3e-34 SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 29 0.89 SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schiz... 27 2.7 SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 27 3.6 SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 25 8.3 SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc... 25 8.3 >SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 395 Score = 139 bits (337), Expect = 3e-34 Identities = 74/184 (40%), Positives = 107/184 (58%) Frame = +2 Query: 107 IFIVGAKRTPFGTFGGVFRNTSATELQTIATVGALKEAGVSPAQVDSMVVGQVISSSQTD 286 ++IV A RTP G+FGG F + AT+L +IA GAL+ + P+ VD + +G V+S++ Sbjct: 6 VYIVSAVRTPMGSFGGSFASLPATKLGSIAIKGALERVNIKPSDVDEVFMGNVVSANL-- 63 Query: 287 GIYTPRHAALKAGIPQEKPVLGINRLCGSGFQSIVNSAQDILTGAAKISVAGGVENMSQA 466 G R AL AG+P+ +N++C SG ++ + AQ I+TG A+I VAGG E+MS A Sbjct: 64 GQNPARQCALGAGLPRSIVCTTVNKVCASGMKATILGAQTIMTGNAEIVVAGGTESMSNA 123 Query: 467 PFAVRNVRFGTALGTNYAFEDTLWAGLSDSYCGLPMGMTAEKLGAQFGITRDEVDNYALQ 646 P+ RFG G + L GLSD+Y GLPMG AE + I R D +A+ Sbjct: 124 PYYAPKNRFGAKYGNVELVDGLLRDGLSDAYDGLPMGNAAELCAEEHSIDRASQDAFAIS 183 Query: 647 SQQK 658 S ++ Sbjct: 184 SYKR 187 >SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces pombe|chr 1|||Manual Length = 949 Score = 28.7 bits (61), Expect = 0.89 Identities = 11/52 (21%), Positives = 27/52 (51%), Gaps = 1/52 (1%) Frame = -1 Query: 430 DLGCAGQNVLSAVHDRLE-TRAAQSVDAEHRLLLRNTSFERRVTWRVDSVSL 278 D + V + ++ T+ + + ++ LLL N+ + +++W +D+V L Sbjct: 177 DFNLDDPQIFDVVSEHIDITQTSDAPNSNRNLLLNNSMLQEKISWYLDTVEL 228 >SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 900 Score = 27.1 bits (57), Expect = 2.7 Identities = 14/50 (28%), Positives = 24/50 (48%) Frame = +3 Query: 240 STAWSWAKSSLHHKLTESTRHVTRRSKLVFLKRSRCSASTDCAALVSSRS 389 +T W + SLH + + TR + LVFL++ + +CA R+ Sbjct: 357 NTYWLYTTDSLHELVVNNE---TREASLVFLEKGDFEKALECANTAKVRN 403 >SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 26.6 bits (56), Expect = 3.6 Identities = 13/40 (32%), Positives = 20/40 (50%) Frame = +2 Query: 134 PFGTFGGVFRNTSATELQTIATVGALKEAGVSPAQVDSMV 253 P G G + + T+ Q I + L V PAQ+DS++ Sbjct: 270 PSGPMGMKTGSVNDTQSQQITLMNVLASQNVPPAQIDSIM 309 >SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 2609 Score = 25.4 bits (53), Expect = 8.3 Identities = 15/53 (28%), Positives = 26/53 (49%) Frame = +2 Query: 377 FQSIVNSAQDILTGAAKISVAGGVENMSQAPFAVRNVRFGTALGTNYAFEDTL 535 F S+ ++ Q L + ++ EN++ +A + F L T Y+FE TL Sbjct: 1088 FPSLFHAIQATLEKVSNVNGKLNSENVAALRYADDTMSFLVTLQTTYSFELTL 1140 >SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1063 Score = 25.4 bits (53), Expect = 8.3 Identities = 12/46 (26%), Positives = 23/46 (50%) Frame = -2 Query: 180 SVADVLRKTPPKVPNGVLLAPTMKIPLTEADMIQNYTYPVNVELKY 43 SV+ +R +PN P P +A+ + N ++PV+ +L + Sbjct: 755 SVSSNVRPINGSIPNAQPSVPGQAQPAAKANSVGNTSFPVDSKLAF 800 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,896,784 Number of Sequences: 5004 Number of extensions: 57365 Number of successful extensions: 183 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 153 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 181 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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