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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0338
         (736 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    55   9e-09
SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pomb...    52   9e-08
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb...    48   2e-06
SPAC3A12.03c |mug145||ubiquitin-protein ligase E3 |Schizosacchar...    47   2e-06
SPAC17G8.10c |dma1||mitotic spindle checkpoint protein Dma1|Schi...    44   3e-05
SPCC126.07c |||human CTD-binding SR-like protein rA9 homolog|Sch...    44   3e-05
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    42   9e-05
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    42   9e-05
SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|ch...    42   9e-05
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    42   9e-05
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce...    40   3e-04
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    39   8e-04
SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    38   0.002
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos...    38   0.002
SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    36   0.008
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    35   0.010
SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    35   0.010
SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb...    34   0.024
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi...    34   0.024
SPAC23H4.18c |rbx1|pip1, pip1|RING-box protein 1|Schizosaccharom...    32   0.098
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom...    31   0.13 
SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3 B...    30   0.39 
SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizo...    29   0.69 
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo...    29   0.69 
SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch...    29   0.91 
SPAC343.03 |apc11||anaphase-promoting complex subunit Apc11|Schi...    28   1.6  
SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosacch...    26   6.4  
SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schiz...    25   8.5  

>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 513

 Score = 55.2 bits (127), Expect = 9e-09
 Identities = 20/43 (46%), Positives = 27/43 (62%)
 Frame = +1

Query: 145 ECSICFEIMLKDQELMALPCTHNFHSQCIMPWLQEKQTCPNCR 273
           EC+IC E+   + +++ LPC H FH  CI PWL+   TC  CR
Sbjct: 395 ECTICMEMFKINDDVIQLPCKHYFHENCIKPWLRVNGTCAICR 437


>SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 372

 Score = 52.0 bits (119), Expect = 9e-08
 Identities = 21/55 (38%), Positives = 30/55 (54%), Gaps = 1/55 (1%)
 Frame = +1

Query: 118 SRKRSWSLEECSICFEIMLKDQELMALPCTHNFHSQCIMPWLQE-KQTCPNCRKD 279
           S +R+    EC IC E   K  +++ALPC H FH  CI  W+ + +  CP C  +
Sbjct: 311 STRRATFGVECVICLESFTKGDKVVALPCKHEFHRPCIAKWIVDYRHACPTCNTE 365


>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 741

 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 19/46 (41%), Positives = 27/46 (58%), Gaps = 2/46 (4%)
 Frame = +1

Query: 148 CSICFEIMLKDQELMA--LPCTHNFHSQCIMPWLQEKQTCPNCRKD 279
           C IC++ M ++ E  A  +PC H F   C+  WL+   TCP CRK+
Sbjct: 107 CPICYDDMNENDEKQATKMPCGHIFGKNCLQKWLENHCTCPLCRKE 152


>SPAC3A12.03c |mug145||ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 309

 Score = 47.2 bits (107), Expect = 2e-06
 Identities = 20/45 (44%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
 Frame = +1

Query: 148 CSICFEIMLKDQELMALPCTHNFHSQCIMPWLQE-KQTCPNCRKD 279
           C IC+     D  L  LPC H FH+QCI  W+   K +CP C +D
Sbjct: 205 CIICYADYAFDDILRVLPCEHVFHTQCIDTWMTTMKASCPLCNED 249


>SPAC17G8.10c |dma1||mitotic spindle checkpoint protein
           Dma1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 267

 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 20/47 (42%), Positives = 26/47 (55%), Gaps = 3/47 (6%)
 Frame = +1

Query: 145 ECSICFEIMLKDQELMALPCTHNFHSQCIMPWLQEKQ---TCPNCRK 276
           EC IC   +L  Q L   PC+H++H +CI P L E     +C  CRK
Sbjct: 191 ECCICLMPVLPCQALFVAPCSHSYHYKCIRPTLNESHPYFSCFICRK 237


>SPCC126.07c |||human CTD-binding SR-like protein rA9
           homolog|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 571

 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 18/74 (24%), Positives = 26/74 (35%)
 Frame = +1

Query: 112 PNSRKRSWSLEECSICFEIMLKDQELMALPCTHNFHSQCIMPWLQEKQTCPNCRKDA*SK 291
           PN     W            L    +  +PC H FH+ C+  W +   TCP CR +    
Sbjct: 26  PNCPLDQWDSSSVPASISSTLDGLRIAKIPCGHYFHNHCLESWCRVANTCPLCRTEFLKV 85

Query: 292 VIKEVKSADWINVF 333
            + E     W   +
Sbjct: 86  DVLEFVKGPWYRAY 99


>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 676

 Score = 41.9 bits (94), Expect = 9e-05
 Identities = 13/35 (37%), Positives = 21/35 (60%)
 Frame = +1

Query: 169 MLKDQELMALPCTHNFHSQCIMPWLQEKQTCPNCR 273
           M+  +  M  PC H +H QC++ W++ +  CP CR
Sbjct: 636 MMVRRNYMLTPCHHLYHRQCLLQWMETRSICPVCR 670


>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 468

 Score = 41.9 bits (94), Expect = 9e-05
 Identities = 16/44 (36%), Positives = 25/44 (56%)
 Frame = +1

Query: 145 ECSICFEIMLKDQELMALPCTHNFHSQCIMPWLQEKQTCPNCRK 276
           EC IC E +   Q      C H +  +C++ WL+E ++CP CR+
Sbjct: 84  ECPICTEAL---QRPFTTHCGHTYCYECLLNWLKESKSCPTCRQ 124


>SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 677

 Score = 41.9 bits (94), Expect = 9e-05
 Identities = 14/27 (51%), Positives = 18/27 (66%)
 Frame = +1

Query: 196 LPCTHNFHSQCIMPWLQEKQTCPNCRK 276
           LPC H  H  C+  WL+ +QTCP CR+
Sbjct: 326 LPCGHILHFHCLRNWLERQQTCPICRR 352


>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 486

 Score = 41.9 bits (94), Expect = 9e-05
 Identities = 24/63 (38%), Positives = 29/63 (46%)
 Frame = +1

Query: 145 ECSICFEIMLKDQELMALPCTHNFHSQCIMPWLQEKQTCPNCRKDA*SKVIKEVKSADWI 324
           EC ICF  ML D   +  PC H F   C+M  L +   CP CR    S V+ E   +  I
Sbjct: 168 ECQICFG-MLYDP--VVSPCGHTFCGPCLMQALTQSPQCPTCRFGLPSPVVLEHAKSHSI 224

Query: 325 NVF 333
             F
Sbjct: 225 TTF 227


>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1610

 Score = 40.3 bits (90), Expect = 3e-04
 Identities = 16/50 (32%), Positives = 27/50 (54%), Gaps = 5/50 (10%)
 Frame = +1

Query: 139  LEECSICFEIMLKDQEL---MALPCTHNFHSQCIMPWLQEKQT--CPNCR 273
            +EEC+IC+ ++  ++ L       C H FH+ C+  W +   +  CP CR
Sbjct: 1555 VEECAICYSVLSVERTLPNKRCGTCRHKFHASCLYKWFKSSNSSRCPLCR 1604


>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 583

 Score = 38.7 bits (86), Expect = 8e-04
 Identities = 18/46 (39%), Positives = 22/46 (47%), Gaps = 2/46 (4%)
 Frame = +1

Query: 142 EECSICFE-IMLKDQELMALPCTHNFHSQCIMPWLQEKQ-TCPNCR 273
           E C +C     L D+      C H FH +CI  WL   Q +CP CR
Sbjct: 523 ERCLVCLSNFELNDECRRLKQCNHFFHRECIDQWLTSSQNSCPLCR 568


>SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 425

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
 Frame = +1

Query: 148 CSICFEIMLKDQE-LMALPCTHNFHSQCIMPWLQEKQTCPNCRK 276
           C IC E M   +E ++ L C+H  H +C   +++    CP C K
Sbjct: 271 CPICGEYMFNSRERVIFLSCSHPLHQRCHEEYIRTNYRCPTCYK 314


>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 556

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 2/46 (4%)
 Frame = +1

Query: 145 ECSICFEIMLKDQELMA-LPCTHNFHSQCIMPW-LQEKQTCPNCRK 276
           EC+IC     ++  L   LPC H FH  CI P+ L+    CP C++
Sbjct: 497 ECTICLCEYSEESPLYRELPCHHIFHPACIDPYLLKNSDLCPLCKQ 542


>SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 268

 Score = 35.5 bits (78), Expect = 0.008
 Identities = 19/56 (33%), Positives = 27/56 (48%), Gaps = 2/56 (3%)
 Frame = +1

Query: 115 NSRKRSWSLEECSICFEIMLKDQELMAL-PCTHN-FHSQCIMPWLQEKQTCPNCRK 276
           NS    +   +C IC +   KD     L PC H+ F   CI  W+ +  TCP C++
Sbjct: 10  NSSNSRYLRRDCVICLQ---KDGLRAQLSPCGHDQFDYSCICRWMDQSLTCPICKR 62


>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 821

 Score = 35.1 bits (77), Expect = 0.010
 Identities = 18/48 (37%), Positives = 20/48 (41%), Gaps = 2/48 (4%)
 Frame = +1

Query: 136 SLEECSICFEIMLKDQELMALP-CTHNFHSQCIMPWLQE-KQTCPNCR 273
           S + C IC E          L  C H FH  CI  WL     +CP CR
Sbjct: 762 SADSCLICLETYTNGDICRKLQACKHFFHQACIDQWLTTGNNSCPLCR 809


>SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 547

 Score = 35.1 bits (77), Expect = 0.010
 Identities = 16/46 (34%), Positives = 21/46 (45%), Gaps = 1/46 (2%)
 Frame = +1

Query: 139 LEECSICFEIMLKD-QELMALPCTHNFHSQCIMPWLQEKQTCPNCR 273
           L  C +C E M      L+ + C H FH  C+  W     +CP CR
Sbjct: 205 LPTCVVCLERMDSSITGLITIVCQHTFHCPCLQKW--GNSSCPVCR 248


>SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 387

 Score = 33.9 bits (74), Expect = 0.024
 Identities = 16/42 (38%), Positives = 21/42 (50%)
 Frame = +1

Query: 148 CSICFEIMLKDQELMALPCTHNFHSQCIMPWLQEKQTCPNCR 273
           C IC E     +  +   C+H F S CI  +L+E   CP CR
Sbjct: 29  CLICHEYF---RAPLITSCSHTFCSFCIRDYLREHPMCPACR 67


>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 306

 Score = 33.9 bits (74), Expect = 0.024
 Identities = 19/52 (36%), Positives = 25/52 (48%), Gaps = 1/52 (1%)
 Frame = +1

Query: 145 ECSICFEIMLKDQELMALPCTHNFHSQCIMPWLQEKQTCPNCRK-DA*SKVI 297
           +CS+C E +       A  C H F   CI  W  +K  CP CR   + SK+I
Sbjct: 255 KCSLCMEFIHCPA---ATECGHIFCWSCINGWTSKKSECPLCRAFSSPSKII 303


>SPAC23H4.18c |rbx1|pip1, pip1|RING-box protein
           1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 107

 Score = 31.9 bits (69), Expect = 0.098
 Identities = 10/21 (47%), Positives = 11/21 (52%)
 Frame = +1

Query: 202 CTHNFHSQCIMPWLQEKQTCP 264
           C H FH  CI  WL  +  CP
Sbjct: 74  CNHAFHFHCISRWLNTRNVCP 94


>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1375

 Score = 31.5 bits (68), Expect = 0.13
 Identities = 13/42 (30%), Positives = 22/42 (52%)
 Frame = +1

Query: 148  CSICFEIMLKDQELMALPCTHNFHSQCIMPWLQEKQTCPNCR 273
            C IC +I+   ++     C H + S C+  WL+   +CP C+
Sbjct: 1092 CIICRDII---KQGFITTCGHLYCSFCLEAWLKHSSSCPMCK 1130


>SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3
           Brl1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 692

 Score = 29.9 bits (64), Expect = 0.39
 Identities = 15/43 (34%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
 Frame = +1

Query: 145 ECSICFEIMLKDQELMALPCTHNFHSQCIMPWLQEK-QTCPNC 270
           +CS+C     K +  +   C H F S C+ P+ + K  TCP C
Sbjct: 638 KCSVCNFSNWKSK--LIPNCGHAFCSNCMEPFYEHKTSTCPQC 678


>SPBC16D10.07c |sir2||Sir2 family histone deacetylase
           Sir2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 475

 Score = 29.1 bits (62), Expect = 0.69
 Identities = 15/27 (55%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
 Frame = +1

Query: 622 KRKLLKIFTQ-ISNAERLFGLSDNSLI 699
           K KL  +FTQ I N E+  GLSDN +I
Sbjct: 237 KNKLSTLFTQNIDNLEKKTGLSDNKII 263


>SPCC18.03 |||shuttle craft like transcriptional
           regulator|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1077

 Score = 29.1 bits (62), Expect = 0.69
 Identities = 12/35 (34%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
 Frame = +1

Query: 139 LEECSICFEIMLKDQELMAL-PCTHNFHSQCIMPW 240
           L ECS+C + +     + +   C H FH  CI  W
Sbjct: 194 LYECSVCTDTINPSTSIWSCGTCYHVFHLSCIRKW 228


>SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 269

 Score = 28.7 bits (61), Expect = 0.91
 Identities = 16/47 (34%), Positives = 22/47 (46%), Gaps = 3/47 (6%)
 Frame = +1

Query: 145 ECSICFEIMLKDQELMALPCTHNFHSQCIMPWL---QEKQTCPNCRK 276
           +C IC +     + L   PC H F + CI+  L      Q CP CR+
Sbjct: 205 KCVICLD---SPENLSCTPCGHIFCNFCILSALGTTAATQKCPVCRR 248


>SPAC343.03 |apc11||anaphase-promoting complex subunit
           Apc11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 94

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 11/34 (32%), Positives = 16/34 (47%)
 Frame = +1

Query: 202 CTHNFHSQCIMPWLQEKQTCPNCRKDA*SKVIKE 303
           C H FH+ CI  WL    +   C  D  + V+ +
Sbjct: 52  CKHIFHAHCIQNWLATSGSQGQCPMDRQTFVVAD 85


>SPBC13E7.02 |cwf24||GCN5-related N
           acetyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 533

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 10/30 (33%), Positives = 15/30 (50%)
 Frame = +1

Query: 190 MALPCTHNFHSQCIMPWLQEKQTCPNCRKD 279
           +A  C H+F  QC +   ++  TC  C  D
Sbjct: 265 IATTCGHHFCEQCAITRYRKTPTCIQCGAD 294


>SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 900

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 13/42 (30%), Positives = 18/42 (42%), Gaps = 2/42 (4%)
 Frame = +1

Query: 115 NSRKRSWSLEECSICFEIM--LKDQELMALPCTHNFHSQCIM 234
           N R R   LE    C+     L  +  +  PC H FH  C++
Sbjct: 823 NMRNRYIVLEPNESCWHCNQPLFSEPFVLFPCQHAFHRSCML 864


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,706,824
Number of Sequences: 5004
Number of extensions: 49975
Number of successful extensions: 124
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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